Restriction map for
pFN21AB3232
(Linear) (Six-base) MAPSORT of: KIBB3232.seq Check: 5366 from: 1 to: 2139
KIBB3232.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1007 1407 2139
Size: 1007 400 732
Fragments arranged by size:
1007 732 400
AccI GT'mk_AC
Cuts at: 0 121 2139
Size: 121 2018
Acc65I G'GTAC_C
Cuts at: 0 66 1418 2139
Size: 66 1352 721
Fragments arranged by size:
1352 721 66
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1747 2139
Size: 1747 392
AflII C'TTAA_G
Cuts at: 0 859 2139
Size: 859 1280
AleI CACnn'nnGTG
Cuts at: 0 1489 2139
Size: 1489 650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 517 688 1342 2139
Size: 517 171 654 797
Fragments arranged by size:
797 654 517 171
AlwNI CAG_nnn'CTG
Cuts at: 0 219 357 548 1503 1584 2139
Size: 219 138 191 955 81 555
Fragments arranged by size:
955 555 219 191 138 81
ApoI r'AATT_y
Cuts at: 0 2133 2139
Size: 2133 6
AvaI C'yCGr_G
Cuts at: 0 323 749 781 928 1357 1432 1727 2139
Size: 323 426 32 147 429 75 295 412
Fragments arranged by size:
429 426 412 323 295 147 75 32
BanI G'GyrC_C
Cuts at: 0 43 66 753 777 1150 1230 1418 2139
Size: 43 23 687 24 373 80 188 721
Fragments arranged by size:
721 687 373 188 80 43 24 23
BanII G_rGCy'C
Cuts at: 0 788 1592 1892 2139
Size: 788 804 300 247
Fragments arranged by size:
804 788 300 247
BbvCI CC'TCA_GC
Cuts at: 0 490 2139
Size: 490 1649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 892 926 2139
Size: 892 34 1213
Fragments arranged by size:
1213 892 34
BglI GCCn_nnn'nGGC
Cuts at: 0 475 1903 2139
Size: 475 1428 236
Fragments arranged by size:
1428 475 236
BglII A'GATC_T
Cuts at: 0 680 1361 2139
Size: 680 681 778
Fragments arranged by size:
778 681 680
BlpI GC'TnA_GC
Cuts at: 0 358 2139
Size: 358 1781
Bme1580I G_kGCm'C
Cuts at: 0 62 758 768 782 1153 1235 1341 2028
Size: 62 696 10 14 371 82 106 687
Cuts at: 2028 2139
Size: 111
Fragments arranged by size:
696 687 371 111 106 82 62 14
10
BmrI ACTGGGnnnn_n'
Cuts at: 0 1377 1788 1812 2139
Size: 1377 411 24 327
Fragments arranged by size:
1377 411 327 24
BmtI G_CTAG'C
Cuts at: 0 31 661 1939 2139
Size: 31 630 1278 200
Fragments arranged by size:
1278 630 200 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 741 912 2006 2139
Size: 741 171 1094 133
Fragments arranged by size:
1094 741 171 133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 718 736 802 1192 1282 1361 1465
Size: 415 303 18 66 390 90 79 104
Cuts at: 1465 1540 1606 1982 2040 2139
Size: 75 66 376 58 99
Fragments arranged by size:
415 390 376 303 104 99 90 79
75 66 66 58 18
Bpu10I CC'TnA_GC
Cuts at: 0 490 2139
Size: 490 1649
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 2139
Size: 772 1367
BsaI GGTCTCn'nnnn_
Cuts at: 0 900 1006 1099 1597 2139
Size: 900 106 93 498 542
Fragments arranged by size:
900 542 498 106 93
BsaWI w'CCGG_w
Cuts at: 0 1200 2139
Size: 1200 939
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 2098 2128 2139
Size: 502 30 1566 30 11
Fragments arranged by size:
1566 502 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 901 1543 1674 2139
Size: 388 513 642 131 465
Fragments arranged by size:
642 513 465 388 131
BseYI C'CCAG_C
Cuts at: 0 264 350 867 1133 1381 1930 2139
Size: 264 86 517 266 248 549 209
Fragments arranged by size:
549 517 266 264 248 209 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 493 838 1618 2139
Size: 349 144 345 780 521
Fragments arranged by size:
780 521 349 345 144
BsiHKAI G_wGCw'C
Cuts at: 0 1551 1592 2139
Size: 1551 41 547
Fragments arranged by size:
1551 547 41
Bsp1286I G_dGCh'C
Cuts at: 0 62 758 768 782 788 1153 1235 1341
Size: 62 696 10 14 6 365 82 106
Cuts at: 1341 1551 1592 1892 2028 2139
Size: 210 41 300 136 111
Fragments arranged by size:
696 365 300 210 136 111 106 82
62 41 14 10 6
BspEI T'CCGG_A
Cuts at: 0 1200 2139
Size: 1200 939
BspHI T'CATG_A
Cuts at: 0 581 2139
Size: 581 1558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 650 680 1007 1088 1407 2139
Size: 650 30 327 81 319 732
Fragments arranged by size:
732 650 327 319 81 30
BsrBI CCG'CTC
Cuts at: 0 131 816 2139
Size: 131 685 1323
Fragments arranged by size:
1323 685 131
BsrDI GCAATG_nn'
Cuts at: 0 171 1329 1892 2139
Size: 171 1158 563 247
Fragments arranged by size:
1158 563 247 171
BssSI C'ACGA_G
Cuts at: 0 10 2139
Size: 10 2129
BstEII G'GTnAC_C
Cuts at: 0 1635 2139
Size: 1635 504
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1713 2139
Size: 1713 426
BstYI r'GATC_y
Cuts at: 0 107 680 1361 2092 2139
Size: 107 573 681 731 47
Fragments arranged by size:
731 681 573 107 47
BstZ17I GTA'TAC
Cuts at: 0 122 2139
Size: 122 2017
Bsu36I CC'TnA_GG
Cuts at: 0 529 1872 2139
Size: 529 1343 267
Fragments arranged by size:
1343 529 267
BtgI C'CryG_G
Cuts at: 0 62 961 1098 1145 1769 2139
Size: 62 899 137 47 624 370
Fragments arranged by size:
899 624 370 137 62 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 450 2139
Size: 450 1689
BtsI GCAGTG_nn'
Cuts at: 0 47 2139
Size: 47 2092
DraIII CAC_nnn'GTG
Cuts at: 0 1187 2139
Size: 1187 952
DrdI GACnn_nn'nnGTC
Cuts at: 0 1065 2139
Size: 1065 1074
EaeI y'GGCC_r
Cuts at: 0 275 419 635 1343 2139
Size: 275 144 216 708 796
Fragments arranged by size:
796 708 275 216 144
EarI CTCTTCn'nnn_
Cuts at: 0 1463 2139
Size: 1463 676
EcoICRI GAG'CTC
Cuts at: 0 1590 2139
Size: 1590 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 718 736 802 1192 1282 1361 1465
Size: 415 303 18 66 390 90 79 104
Cuts at: 1465 1540 1606 1747 1982 2040 2139
Size: 75 66 141 235 58 99
Fragments arranged by size:
415 390 303 235 141 104 99 90
79 75 66 66 58 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1084 1762 2139
Size: 1084 678 377
Fragments arranged by size:
1084 678 377
EcoO109I rG'GnC_Cy
Cuts at: 0 476 1439 2139
Size: 476 963 700
Fragments arranged by size:
963 700 476
HaeII r_GCGC'y
Cuts at: 0 1215 2139
Size: 1215 924
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1162 1194 2139
Size: 1162 32 945
Fragments arranged by size:
1162 945 32
HindIII A'AGCT_T
Cuts at: 0 1281 2139
Size: 1281 858
KpnI G_GTAC'C
Cuts at: 0 70 1422 2139
Size: 70 1352 717
Fragments arranged by size:
1352 717 70
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 710 2139
Size: 545 165 1429
Fragments arranged by size:
1429 545 165
MscI TGG'CCA
Cuts at: 0 277 421 637 2139
Size: 277 144 216 1502
Fragments arranged by size:
1502 277 216 144
MslI CAynn'nnrTG
Cuts at: 0 1489 2139
Size: 1489 650
MspA1I CmG'CkG
Cuts at: 0 354 384 548 744 822 1115 1133 1381
Size: 354 30 164 196 78 293 18 248
Cuts at: 1381 1930 2139
Size: 549 209
Fragments arranged by size:
549 354 293 248 209 196 164 78
30 18
NheI G'CTAG_C
Cuts at: 0 27 657 1935 2139
Size: 27 630 1278 204
Fragments arranged by size:
1278 630 204 27
NspI r_CATG'y
Cuts at: 0 79 852 2139
Size: 79 773 1287
Fragments arranged by size:
1287 773 79
PasI CC'CwG_GG
Cuts at: 0 473 2139
Size: 473 1666
PflMI CCAn_nnn'nTGG
Cuts at: 0 1503 2139
Size: 1503 636
PfoI T'CCnGG_A
Cuts at: 0 611 1238 2139
Size: 611 627 901
Fragments arranged by size:
901 627 611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 425 457 2139
Size: 425 32 1682
Fragments arranged by size:
1682 425 32
PpuMI rG'GwC_Cy
Cuts at: 0 1439 2139
Size: 1439 700
PshAI GACnn'nnGTC
Cuts at: 0 184 2139
Size: 184 1955
PstI C_TGCA'G
Cuts at: 0 230 1085 2139
Size: 230 855 1054
Fragments arranged by size:
1054 855 230
PvuII CAG'CTG
Cuts at: 0 354 384 744 1381 1930 2139
Size: 354 30 360 637 549 209
Fragments arranged by size:
637 549 360 354 209 30
RsrII CG'GwC_CG
Cuts at: 0 1221 2139
Size: 1221 918
SacI G_AGCT'C
Cuts at: 0 1592 2139
Size: 1592 547
SanDI GG'GwC_CC
Cuts at: 0 1439 2139
Size: 1439 700
SbfI CC_TGCA'GG
Cuts at: 0 1085 2139
Size: 1085 1054
ScaI AGT'ACT
Cuts at: 0 1777 2139
Size: 1777 362
SexAI A'CCwGG_T
Cuts at: 0 1502 1669 2139
Size: 1502 167 470
Fragments arranged by size:
1502 470 167
SfcI C'TryA_G
Cuts at: 0 226 427 1081 2139
Size: 226 201 654 1058
Fragments arranged by size:
1058 654 226 201
SmaI CCC'GGG
Cuts at: 0 751 783 2139
Size: 751 32 1356
Fragments arranged by size:
1356 751 32
SmlI C'TyrA_G
Cuts at: 0 787 859 2139
Size: 787 72 1280
Fragments arranged by size:
1280 787 72
SrfI GCCC'GGGC
Cuts at: 0 783 2139
Size: 783 1356
StyI C'CwwG_G
Cuts at: 0 1947 2139
Size: 1947 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 1659 1831 1966 2139
Size: 24 1635 172 135 173
Fragments arranged by size:
1635 173 172 135 24
TatI w'GTAC_w
Cuts at: 0 1775 2139
Size: 1775 364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 502 534 1268 1300 2139
Size: 502 32 734 32 839
Fragments arranged by size:
839 734 502 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1123 2139
Size: 1123 1016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 765 1306 1971 2139
Size: 60 705 541 665 168
Fragments arranged by size:
705 665 541 168 60
XmaI C'CCGG_G
Cuts at: 0 749 781 2139
Size: 749 32 1358
Fragments arranged by size:
1358 749 32
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AleI AlfI AlwNI
ApoI AvaI BanI BanII BbvCI BcgI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BssSI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HindIII KpnI MmeI MscI MslI MspA1I NheI NspI
PasI PflMI PfoI PpiI PpuMI PshAI PstI PvuII
RsrII SacI SanDI SbfI ScaI SexAI SfcI SmaI
SmlI SrfI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BciVI BclI BfrBI BmgBI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmI BsmBI BsrFI BsrGI BssHII BstAPI
BstBI ClaI CspCI DraI EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PciI PmeI PmlI PsiI PspOMI PspXI
PsrI PvuI SacII SalI SapI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI XbaI
XhoI XmnI ZraI