(Circular) (Six-base) MAPSORT of: pFN21AB3224.seq Check: 2568 from: 1 to: 6946 pFN21AB3224 6946 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4756 278 Size: 53 83 186 936 3220 2468 Fragments arranged by size: 3220 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2207 4289 1937 Size: 270 2082 4594 Fragments arranged by size: 4594 2082 270 Acc65I G'GTAC_C Cuts at: 4267 4267 Size: 6946 AclI AA'CG_TT Cuts at: 1563 5073 5446 1563 Size: 3510 373 3063 Fragments arranged by size: 3510 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2438 2499 2528 2706 2853 3045 3876 4143 Size: 806 61 29 178 147 192 831 267 Cuts at: 4143 5009 6393 6661 1632 Size: 866 1384 268 1917 Fragments arranged by size: 1917 1384 866 831 806 268 267 192 178 147 61 29 AflII C'TTAA_G Cuts at: 820 1017 3134 820 Size: 197 2117 4632 Fragments arranged by size: 4632 2117 197 AflIII A'CryG_T Cuts at: 1203 3059 5845 6822 1203 Size: 1856 2786 977 1327 Fragments arranged by size: 2786 1856 1327 977 AgeI A'CCGG_T Cuts at: 5777 5777 Size: 6946 AhdI GACnn_n'nnGTC Cuts at: 3220 5675 3220 Size: 2455 4491 AleI CACnn'nnGTG Cuts at: 1166 2078 1166 Size: 912 6034 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3009 1184 Size: 1825 5121 AlwNI CAG_nnn'CTG Cuts at: 2103 2336 3002 4689 6261 2103 Size: 233 666 1687 1572 2788 Fragments arranged by size: 2788 1687 1572 666 233 ApaLI G'TGCA_C Cuts at: 5003 6159 5003 Size: 1156 5790 ApoI r'AATT_y Cuts at: 1493 4255 4481 4633 6742 1493 Size: 2762 226 152 2109 1697 Fragments arranged by size: 2762 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5500 160 Size: 872 4468 1606 Fragments arranged by size: 4468 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 AvaI C'yCGr_G Cuts at: 1870 1943 4271 1870 Size: 73 2328 4545 Fragments arranged by size: 4545 2328 73 BamHI G'GATC_C Cuts at: 4012 4276 4643 4012 Size: 264 367 6315 Fragments arranged by size: 6315 367 264 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4267 5722 618 Size: 325 217 627 2480 1455 1842 Fragments arranged by size: 2480 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2861 4265 1391 Size: 1470 1404 4072 Fragments arranged by size: 4072 1470 1404 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5975 BbvCI CC'TCA_GC Cuts at: 3267 3267 Size: 6946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2703 2737 5137 5171 1903 Size: 34 50 34 682 34 2400 34 3678 Fragments arranged by size: 3678 2400 682 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3049 3671 4838 6048 3049 Size: 622 1167 1210 3947 Fragments arranged by size: 3947 1210 1167 622 BclI T'GATC_A Cuts at: 1548 2170 2953 1548 Size: 622 783 5541 Fragments arranged by size: 5541 783 622 BfrBI ATG'CAT Cuts at: 2843 2843 Size: 6946 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2142 5557 136 Size: 107 122 71 631 605 470 3415 1525 Fragments arranged by size: 3415 1525 631 605 470 122 107 71 BglII A'GATC_T Cuts at: 6941 6941 Size: 6946 BlpI GC'TnA_GC Cuts at: 3522 4363 3522 Size: 841 6105 Bme1580I G_kGCm'C Cuts at: 1790 5007 6163 1790 Size: 3217 1156 2573 Fragments arranged by size: 3217 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6946 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2232 2343 2449 Size: 416 53 83 399 24 815 111 106 Cuts at: 2449 3761 4677 5635 442 Size: 1312 916 958 1753 Fragments arranged by size: 1753 1312 958 916 815 416 399 111 106 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6946 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2628 2813 3528 4022 5606 1239 Size: 842 547 185 715 494 1584 2579 Fragments arranged by size: 2579 1584 842 715 547 494 185 Bpu10I CC'TnA_GC Cuts at: 3267 3267 Size: 6946 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3941 5071 5936 6234 3941 Size: 1130 865 298 4653 Fragments arranged by size: 4653 1130 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2557 3988 5609 882 Size: 617 1058 1431 1621 2219 Fragments arranged by size: 2219 1621 1431 1058 617 BsaAI yAC'GTr Cuts at: 493 1204 4737 493 Size: 711 3533 2702 Fragments arranged by size: 3533 2702 711 BsaBI GATnn'nnATC Cuts at: 6940 6940 Size: 6946 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4753 5135 5770 275 Size: 53 83 186 936 3220 382 635 1451 Fragments arranged by size: 3220 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5379 5777 6051 6198 6609 5379 Size: 398 274 147 411 5716 Fragments arranged by size: 5716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2554 2584 1178 Size: 30 165 30 1151 30 5540 Fragments arranged by size: 5540 1151 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2493 2575 1186 Size: 1307 82 5557 Fragments arranged by size: 5557 1307 82 BseYI C'CCAG_C Cuts at: 2261 3139 4191 6149 6645 2261 Size: 878 1052 1958 496 2562 Fragments arranged by size: 2562 1958 1052 878 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6373 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4396 5157 6185 1540 Size: 275 186 2395 761 1028 2301 Fragments arranged by size: 2395 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5007 5092 6163 5007 Size: 85 1071 5790 Fragments arranged by size: 5790 1071 85 BsmI GAATG_Cn' Cuts at: 2726 4457 4550 2726 Size: 1731 93 5122 Fragments arranged by size: 5122 1731 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2861 4265 5007 5092 6163 1391 Size: 399 1071 1404 742 85 1071 2174 Fragments arranged by size: 2174 1404 1071 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6609 6609 Size: 6946 BspHI T'CATG_A Cuts at: 3313 4835 6565 3313 Size: 1522 1730 3694 Fragments arranged by size: 3694 1730 1522 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3517 4301 844 Size: 2673 784 3489 Fragments arranged by size: 3489 2673 784 BsrBI CCG'CTC Cuts at: 1394 1823 4833 1394 Size: 429 3010 3507 Fragments arranged by size: 3507 3010 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2861 3333 3943 5441 5615 65 Size: 1183 1613 472 610 1498 174 1396 Fragments arranged by size: 1613 1498 1396 1183 610 472 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2965 5590 5777 1700 Size: 110 1155 2625 187 2869 Fragments arranged by size: 2869 2625 1155 187 110 BsrGI T'GTAC_A Cuts at: 96 2462 3569 96 Size: 2366 1107 3473 Fragments arranged by size: 3473 2366 1107 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6946 BssSI C'ACGA_G Cuts at: 5006 6018 5006 Size: 1012 5934 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6946 BstBI TT'CG_AA Cuts at: 5750 5750 Size: 6946 BstEII G'GTnAC_C Cuts at: 1184 2080 1184 Size: 896 6050 BstXI CCAn_nnnn'nTGG Cuts at: 3883 3883 Size: 6946 BstYI r'GATC_y Cuts at: 1972 2761 4012 4276 4643 5028 5045 6497 6583 Size: 789 1251 264 367 385 17 1452 86 Cuts at: 6583 6595 6941 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1251 789 385 367 346 264 86 17 12 BstZ17I GTA'TAC Cuts at: 2208 2208 Size: 6946 BtgI C'CryG_G Cuts at: 513 1065 2137 2787 513 Size: 552 1072 650 4672 Fragments arranged by size: 4672 1072 650 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6207 Fragments arranged by size: 6207 683 56 BtsI GCAGTG_nn' Cuts at: 2152 3981 4462 5256 5276 2152 Size: 1829 481 794 20 3822 Fragments arranged by size: 3822 1829 794 481 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6946 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3936 3971 529 Size: 35 3372 35 3504 Fragments arranged by size: 3504 3372 35 35 DraI TTT'AAA Cuts at: 2250 4250 2250 Size: 2000 4946 DraIII CAC_nnn'GTG Cuts at: 2226 2351 2226 Size: 125 6821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5953 809 Size: 507 4637 1802 Fragments arranged by size: 4637 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2737 4393 5282 Size: 54 1425 50 275 18 907 1656 889 Cuts at: 5282 6911 8 Size: 1629 43 Fragments arranged by size: 1656 1629 1425 907 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4393 1537 Size: 275 2581 4090 Fragments arranged by size: 4090 2581 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2976 3681 4876 1353 Size: 1623 705 1195 3423 Fragments arranged by size: 3423 1623 1195 705 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5533 5907 6053 387 Size: 1064 138 3944 374 146 1280 Fragments arranged by size: 3944 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2438 2499 2528 2628 2706 2813 Size: 393 449 357 61 29 100 78 107 Cuts at: 2813 2853 3045 3528 3876 4022 4143 5009 5606 Size: 40 192 483 348 146 121 866 597 Cuts at: 5606 6393 6661 1239 Size: 787 268 1524 Fragments arranged by size: 1524 866 787 597 483 449 393 357 348 268 192 146 121 107 100 78 61 40 29 EcoRI G'AATT_C Cuts at: 4255 4633 4255 Size: 378 6568 EcoRV GAT'ATC Cuts at: 2904 2904 Size: 6946 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3211 3680 3211 Size: 469 6477 FspI TGC'GCA Cuts at: 3597 5452 3597 Size: 1855 5091 FspAI rTGC'GCAy Cuts at: 3597 3597 Size: 6946 HaeII r_GCGC'y Cuts at: 1678 6093 1678 Size: 4415 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2100 2132 2554 2586 3206 Size: 32 135 32 528 32 422 32 620 Cuts at: 3206 3238 3395 3427 3645 3677 5587 5619 5661 Size: 32 157 32 218 32 1910 32 42 Cuts at: 5661 5693 1373 Size: 32 2626 Fragments arranged by size: 2626 1910 620 528 422 218 157 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3772 4290 4536 669 Size: 988 281 1834 518 246 3079 Fragments arranged by size: 3079 1834 988 518 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6946 HpaI GTT'AAC Cuts at: 4536 4536 Size: 6946 KpnI G_GTAC'C Cuts at: 4271 4271 Size: 6946 MfeI C'AATT_G Cuts at: 4545 4545 Size: 6946 MluI A'CGCG_T Cuts at: 6822 6822 Size: 6946 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2250 2578 3631 3654 4250 6060 6244 1305 Size: 945 328 1053 23 596 1810 184 2007 Fragments arranged by size: 2007 1810 1053 945 596 328 184 23 MscI TGG'CCA Cuts at: 10 64 1489 1832 2739 10 Size: 54 1425 343 907 4217 Fragments arranged by size: 4217 1425 907 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 4904 5263 5422 518 Size: 648 912 2826 359 159 2042 Fragments arranged by size: 2826 2042 912 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2078 2103 2139 3266 3854 4362 5039 6187 Size: 472 25 36 1127 588 508 677 1148 Cuts at: 6187 6432 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1127 677 588 508 472 245 36 25 NcoI C'CATG_G Cuts at: 513 1065 2787 513 Size: 552 1722 4672 Fragments arranged by size: 4672 1722 552 NdeI CA'TA_TG Cuts at: 387 3715 387 Size: 3328 3618 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6946 NotI GC'GGCC_GC Cuts at: 4393 4393 Size: 6946 NsiI A_TGCA'T Cuts at: 2845 2845 Size: 6946 NspI r_CATG'y Cuts at: 1132 1229 2843 3063 3602 3631 4304 5849 1132 Size: 97 1614 220 539 29 673 1545 2229 Fragments arranged by size: 2229 1614 1545 673 539 220 97 29 PasI CC'CwG_GG Cuts at: 1348 3439 1348 Size: 2091 4855 PciI A'CATG_T Cuts at: 3059 5845 3059 Size: 2786 4160 PflMI CCAn_nnn'nTGG Cuts at: 1108 2656 3827 1108 Size: 1548 1171 4227 Fragments arranged by size: 4227 1548 1171 PfoI T'CCnGG_A Cuts at: 3383 3383 Size: 6946 PmeI GTTT'AAAC Cuts at: 4250 4250 Size: 6946 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5015 5047 6528 6560 5015 Size: 32 1481 32 5401 Fragments arranged by size: 5401 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6946 PsiI TTA'TAA Cuts at: 4516 4516 Size: 6946 PstI C_TGCA'G Cuts at: 830 4298 830 Size: 3468 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6946 PvuII CAG'CTG Cuts at: 2103 3266 3854 2103 Size: 1163 588 5195 Fragments arranged by size: 5195 1163 588 SacII CC_GC'GG Cuts at: 2140 2140 Size: 6946 SalI G'TCGA_C Cuts at: 1936 4288 1936 Size: 2352 4594 SapI GCTCTTCn'nnn_ Cuts at: 2976 2976 Size: 6946 SbfI CC_TGCA'GG Cuts at: 4298 4298 Size: 6946 ScaI AGT'ACT Cuts at: 2276 3296 5194 6906 2276 Size: 1020 1898 1712 2316 Fragments arranged by size: 2316 1898 1712 1020 SfcI C'TryA_G Cuts at: 826 1045 2415 3090 4294 5429 6110 6301 826 Size: 219 1370 675 1204 1135 681 191 1471 Fragments arranged by size: 1471 1370 1204 1135 681 675 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6946 SmaI CCC'GGG Cuts at: 1872 4273 1872 Size: 2401 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3134 3920 5050 5951 6213 820 Size: 197 926 1191 786 1130 901 262 1553 Fragments arranged by size: 1553 1191 1130 926 901 786 262 197 SnaBI TAC'GTA Cuts at: 493 4737 493 Size: 4244 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6946 SphI G_CATG'C Cuts at: 1132 2843 4304 1132 Size: 1711 1461 3774 Fragments arranged by size: 3774 1711 1461 SspI AAT'ATT Cuts at: 5 52 4169 4870 5 Size: 47 4117 701 2081 Fragments arranged by size: 4117 2081 701 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6946 StyI C'CwwG_G Cuts at: 513 1065 2751 2787 3740 4154 4385 513 Size: 552 1686 36 953 414 231 3074 Fragments arranged by size: 3074 1686 953 552 414 231 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2214 2362 3452 3711 3743 4973 4990 Size: 1255 634 148 1090 259 32 1230 17 Cuts at: 4990 5143 5328 5773 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1230 1090 634 445 259 185 153 148 32 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 2232 2274 Size: 274 80 33 51 1443 186 69 42 Cuts at: 2274 2462 3294 3569 3690 5192 6904 96 Size: 188 832 275 121 1502 1712 138 Fragments arranged by size: 1712 1502 1443 832 275 274 188 186 138 121 80 69 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3571 3603 6906 6938 3571 Size: 32 3303 32 3579 Fragments arranged by size: 3579 3303 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2568 1316 Size: 1252 5694 XbaI T'CTAG_A Cuts at: 2294 4282 2294 Size: 1988 4958 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2924 2924 Size: 6946 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6946 XmaI C'CCGG_G Cuts at: 1870 4271 1870 Size: 2401 4545 XmnI GAAnn'nnTTC Cuts at: 2724 3372 5075 2724 Size: 648 1703 4595 Fragments arranged by size: 4595 1703 648 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4754 276 Size: 53 83 186 936 3220 2468 Fragments arranged by size: 3220 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoO109I FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI