(Circular) (Six-base) MAPSORT of: pFN21AB3092.seq Check: 3277 from: 1 to: 6853 pFN21AB3092 6853 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2485 4663 278 Size: 53 83 186 936 949 2178 2468 Fragments arranged by size: 2468 2178 949 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2492 4196 1937 Size: 555 1704 4594 Fragments arranged by size: 4594 1704 555 Acc65I G'GTAC_C Cuts at: 4174 4174 Size: 6853 AclI AA'CG_TT Cuts at: 1563 4980 5353 1563 Size: 3417 373 3063 Fragments arranged by size: 3417 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2405 2637 4916 6300 6568 1632 Size: 773 232 2279 1384 268 1917 Fragments arranged by size: 2279 1917 1384 773 268 232 AflII C'TTAA_G Cuts at: 820 1017 2185 820 Size: 197 1168 5488 Fragments arranged by size: 5488 1168 197 AflIII A'CryG_T Cuts at: 1203 2851 3721 5752 6729 1203 Size: 1648 870 2031 977 1327 Fragments arranged by size: 2031 1648 1327 977 870 AgeI A'CCGG_T Cuts at: 5684 5684 Size: 6853 AhdI GACnn_n'nnGTC Cuts at: 2481 5582 2481 Size: 3101 3752 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6853 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6853 AlwNI CAG_nnn'CTG Cuts at: 2981 4596 6168 2981 Size: 1615 1572 3666 Fragments arranged by size: 3666 1615 1572 ApaLI G'TGCA_C Cuts at: 4910 6066 4910 Size: 1156 5697 ApoI r'AATT_y Cuts at: 1493 2900 2921 2955 3053 3375 3561 3905 4162 Size: 1407 21 34 98 322 186 344 257 Cuts at: 4162 4388 4540 6649 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1407 344 322 257 226 186 152 98 34 21 AseI AT'TA_AT Cuts at: 160 1032 5407 160 Size: 872 4375 1606 Fragments arranged by size: 4375 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 AvaI C'yCGr_G Cuts at: 1870 1943 2448 3000 3438 4178 1870 Size: 73 505 552 438 740 4545 Fragments arranged by size: 4545 740 552 505 438 73 BamHI G'GATC_C Cuts at: 3047 4183 4550 3047 Size: 1136 367 5350 Fragments arranged by size: 5350 1136 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4174 5629 618 Size: 325 217 627 2387 1455 1842 Fragments arranged by size: 2387 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2398 3769 4172 1391 Size: 1007 1371 403 4072 Fragments arranged by size: 4072 1371 1007 403 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5882 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2008 2042 5044 5078 1903 Size: 34 71 34 3002 34 3678 Fragments arranged by size: 3678 3002 71 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4745 5955 4745 Size: 1210 5643 BclI T'GATC_A Cuts at: 1548 2227 3409 1548 Size: 679 1182 4992 Fragments arranged by size: 4992 1182 679 BfrBI ATG'CAT Cuts at: 2633 3190 2633 Size: 557 6296 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3706 5464 136 Size: 107 122 71 631 605 2034 1758 1525 Fragments arranged by size: 2034 1758 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2416 6848 2416 Size: 4432 2421 BlpI GC'TnA_GC Cuts at: 4270 4270 Size: 6853 Bme1580I G_kGCm'C Cuts at: 1790 4914 6070 1790 Size: 3124 1156 2573 Fragments arranged by size: 3124 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6853 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4584 5542 442 Size: 416 53 83 399 24 3167 958 1753 Fragments arranged by size: 3167 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6853 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2471 3563 5513 1239 Size: 1232 1092 1950 2579 Fragments arranged by size: 2579 1950 1232 1092 Bpu10I CC'TnA_GC Cuts at: 2148 2148 Size: 6853 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2655 4148 4978 5843 6141 2655 Size: 1493 830 865 298 3367 Fragments arranged by size: 3367 1493 865 830 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5516 882 Size: 617 4017 2219 Fragments arranged by size: 4017 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4644 493 Size: 711 3440 2702 Fragments arranged by size: 3440 2702 711 BsaBI GATnn'nnATC Cuts at: 6847 6847 Size: 6853 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2482 4660 5042 5677 Size: 53 83 186 936 949 2178 382 635 Cuts at: 5677 275 Size: 1451 Fragments arranged by size: 2178 1451 949 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2787 3458 5286 5684 5958 6105 6516 2787 Size: 671 1828 398 274 147 411 3124 Fragments arranged by size: 3124 1828 671 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2100 2130 2469 2499 3530 Size: 30 165 30 697 30 339 30 1031 Cuts at: 3530 3560 3792 3822 1178 Size: 30 232 30 4209 Fragments arranged by size: 4209 1031 697 339 232 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3830 1186 Size: 2644 4209 BseYI C'CCAG_C Cuts at: 2162 3768 6056 6552 2162 Size: 1606 2288 496 2463 Fragments arranged by size: 2463 2288 1606 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2292 1163 Size: 573 556 5724 Fragments arranged by size: 5724 573 556 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4303 5064 6092 1540 Size: 275 186 2302 761 1028 2301 Fragments arranged by size: 2302 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 2398 4914 4999 6070 2024 Size: 374 2516 85 1071 2807 Fragments arranged by size: 2807 2516 1071 374 85 BsmI GAATG_Cn' Cuts at: 3127 4364 4457 3127 Size: 1237 93 5523 Fragments arranged by size: 5523 1237 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2489 2489 Size: 6853 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2398 3769 4172 4914 4999 6070 Size: 399 234 374 1371 403 742 85 1071 Cuts at: 6070 1391 Size: 2174 Fragments arranged by size: 2174 1371 1071 742 403 399 374 234 85 BspEI T'CCGG_A Cuts at: 2787 3458 6516 2787 Size: 671 3058 3124 Fragments arranged by size: 3124 3058 671 BspHI T'CATG_A Cuts at: 2595 3628 4742 6472 2595 Size: 1033 1114 1730 2976 Fragments arranged by size: 2976 1730 1114 1033 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4208 844 Size: 3364 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4740 1394 Size: 429 2917 3507 Fragments arranged by size: 3507 2917 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3678 5348 5522 65 Size: 1183 2430 1670 174 1396 Fragments arranged by size: 2430 1670 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5497 5684 1700 Size: 110 3687 187 2869 Fragments arranged by size: 3687 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6853 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6853 BssSI C'ACGA_G Cuts at: 4913 5925 4913 Size: 1012 5841 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6853 BstBI TT'CG_AA Cuts at: 5657 5657 Size: 6853 BstEII G'GTnAC_C Cuts at: 1184 3526 3898 1184 Size: 2342 372 4139 Fragments arranged by size: 4139 2342 372 BstYI r'GATC_y Cuts at: 1972 2416 2818 3047 4183 4550 4935 4952 6404 Size: 444 402 229 1136 367 385 17 1452 Cuts at: 6404 6490 6502 6848 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1136 444 402 385 367 346 229 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2702 2702 Size: 6853 BtgI C'CryG_G Cuts at: 513 1065 3701 513 Size: 552 2636 3665 Fragments arranged by size: 3665 2636 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6114 Fragments arranged by size: 6114 683 56 BtsI GCAGTG_nn' Cuts at: 2173 4369 5163 5183 2173 Size: 2196 794 20 3843 Fragments arranged by size: 3843 2196 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6853 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6818 DraI TTT'AAA Cuts at: 2458 2638 3244 4157 2458 Size: 180 606 913 5154 Fragments arranged by size: 5154 913 606 180 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5860 809 Size: 507 4544 1802 Fragments arranged by size: 4544 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2429 4300 5189 Size: 54 1425 50 275 18 599 1871 889 Cuts at: 5189 6818 8 Size: 1629 43 Fragments arranged by size: 1871 1629 1425 889 599 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4300 1537 Size: 275 2488 4090 Fragments arranged by size: 4090 2488 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2403 2729 3164 3799 4783 1353 Size: 1050 326 435 635 984 3423 Fragments arranged by size: 3423 1050 984 635 435 326 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5440 5814 5960 387 Size: 1064 138 3851 374 146 1280 Fragments arranged by size: 3851 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2396 2396 Size: 6853 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2405 2471 2637 3563 4916 5513 6300 Size: 393 773 66 166 926 1353 597 787 Cuts at: 6300 6568 1239 Size: 268 1524 Fragments arranged by size: 1524 1353 926 787 773 597 393 268 166 66 EcoO109I rG'GnC_Cy Cuts at: 2191 3572 2191 Size: 1381 5472 EcoRI G'AATT_C Cuts at: 2900 2955 4162 4540 2900 Size: 55 1207 378 5213 Fragments arranged by size: 5213 1207 378 55 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2975 2975 Size: 6853 FspI TGC'GCA Cuts at: 5359 5359 Size: 6853 HaeII r_GCGC'y Cuts at: 1678 2531 6000 1678 Size: 853 3469 2531 Fragments arranged by size: 3469 2531 853 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2467 2499 2552 2584 3199 Size: 32 135 32 895 32 53 32 615 Cuts at: 3199 3231 5494 5526 5568 5600 1373 Size: 32 2263 32 42 32 2626 Fragments arranged by size: 2626 2263 895 615 135 53 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4197 4443 669 Size: 988 281 2259 246 3079 Fragments arranged by size: 3079 2259 988 281 246 HindIII A'AGCT_T Cuts at: 748 3923 4077 748 Size: 3175 154 3524 Fragments arranged by size: 3524 3175 154 HpaI GTT'AAC Cuts at: 4443 4443 Size: 6853 KpnI G_GTAC'C Cuts at: 4178 4178 Size: 6853 MfeI C'AATT_G Cuts at: 4452 4452 Size: 6853 MluI A'CGCG_T Cuts at: 6729 6729 Size: 6853 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2947 3450 4122 5967 6151 1305 Size: 1642 503 672 1845 184 2007 Fragments arranged by size: 2007 1845 1642 672 503 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2431 10 Size: 54 1425 343 599 4432 Fragments arranged by size: 4432 1425 599 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2136 2628 4811 5170 5329 518 Size: 648 970 492 2183 359 159 2042 Fragments arranged by size: 2183 2042 970 648 492 359 159 MspA1I CmG'CkG Cuts at: 1606 2233 3506 3772 4269 4946 6094 6339 1606 Size: 627 1273 266 497 677 1148 245 2120 Fragments arranged by size: 2120 1273 1148 677 627 497 266 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6301 NdeI CA'TA_TG Cuts at: 387 4018 387 Size: 3631 3222 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6853 NotI GC'GGCC_GC Cuts at: 4300 4300 Size: 6853 NsiI A_TGCA'T Cuts at: 2635 3192 2635 Size: 557 6296 NspI r_CATG'y Cuts at: 1132 1229 2855 3334 3725 4211 5756 1132 Size: 97 1626 479 391 486 1545 2229 Fragments arranged by size: 2229 1626 1545 486 479 391 97 PasI CC'CwG_GG Cuts at: 1348 2975 1348 Size: 1627 5226 PciI A'CATG_T Cuts at: 2851 3721 5752 2851 Size: 870 2031 3952 Fragments arranged by size: 3952 2031 870 PflMI CCAn_nnn'nTGG Cuts at: 1108 2981 3716 1108 Size: 1873 735 4245 Fragments arranged by size: 4245 1873 735 PfoI T'CCnGG_A Cuts at: 2251 2251 Size: 6853 PmeI GTTT'AAAC Cuts at: 4157 4157 Size: 6853 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2127 2159 4922 4954 6435 6467 2127 Size: 32 2763 32 1481 32 2513 Fragments arranged by size: 2763 2513 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2191 2191 Size: 6853 PshAI GACnn'nnGTC Cuts at: 1613 3724 1613 Size: 2111 4742 PsiI TTA'TAA Cuts at: 4423 4423 Size: 6853 PspXI vC'TCGA_Gb Cuts at: 3438 3438 Size: 6853 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2347 2379 2347 Size: 32 6821 PstI C_TGCA'G Cuts at: 830 2815 4205 830 Size: 1985 1390 3478 Fragments arranged by size: 3478 1985 1390 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6853 PvuII CAG'CTG Cuts at: 2233 3506 3772 2233 Size: 1273 266 5314 Fragments arranged by size: 5314 1273 266 SacI G_AGCT'C Cuts at: 2398 2398 Size: 6853 SalI G'TCGA_C Cuts at: 1936 4195 1936 Size: 2259 4594 SapI GCTCTTCn'nnn_ Cuts at: 2403 2403 Size: 6853 SbfI CC_TGCA'GG Cuts at: 4205 4205 Size: 6853 ScaI AGT'ACT Cuts at: 2426 5101 6813 2426 Size: 2675 1712 2466 Fragments arranged by size: 2675 2466 1712 SfcI C'TryA_G Cuts at: 826 1045 2811 3940 4201 5336 6017 6208 826 Size: 219 1766 1129 261 1135 681 191 1471 Fragments arranged by size: 1766 1471 1135 1129 681 261 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6853 SmaI CCC'GGG Cuts at: 1872 4180 1872 Size: 2308 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2185 2670 3438 4127 4957 5858 Size: 197 926 242 485 768 689 830 901 Cuts at: 5858 6120 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 830 768 689 485 262 242 197 SnaBI TAC'GTA Cuts at: 493 4644 493 Size: 4151 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6853 SphI G_CATG'C Cuts at: 1132 4211 1132 Size: 3079 3774 SspI AAT'ATT Cuts at: 5 52 4777 5 Size: 47 4725 2081 Fragments arranged by size: 4725 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6853 StyI C'CwwG_G Cuts at: 513 1065 2194 3347 4292 513 Size: 552 1129 1153 945 3074 Fragments arranged by size: 3074 1153 1129 945 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3062 4880 4897 5050 5235 5680 325 Size: 1255 1482 1818 17 153 185 445 1498 Fragments arranged by size: 1818 1498 1482 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2354 2424 5099 Size: 274 80 33 51 1443 377 70 2675 Cuts at: 5099 6811 96 Size: 1712 138 Fragments arranged by size: 2675 1712 1443 377 274 138 80 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2469 2501 3792 3824 6813 6845 2469 Size: 32 1291 32 2989 32 2477 Fragments arranged by size: 2989 2477 1291 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6853 XbaI T'CTAG_A Cuts at: 4189 4189 Size: 6853 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3538 3538 Size: 6853 XhoI C'TCGA_G Cuts at: 1943 3438 1943 Size: 1495 5358 XmaI C'CCGG_G Cuts at: 1870 4178 1870 Size: 2308 4545 XmnI GAAnn'nnTTC Cuts at: 2658 4982 2658 Size: 2324 4529 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2483 4661 276 Size: 53 83 186 936 949 2178 2468 Fragments arranged by size: 2468 2178 949 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI