(Circular) (Six-base) MAPSORT of: pFN21AB3046.seq Check: 8700 from: 1 to: 9226 pFN21AB3046 9226 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5612 5612 Size: 9226 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7036 278 Size: 53 83 186 936 5500 2468 Fragments arranged by size: 5500 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2694 3471 6569 1937 Size: 757 777 3098 4594 Fragments arranged by size: 4594 3098 777 757 Acc65I G'GTAC_C Cuts at: 2262 2555 6547 2262 Size: 293 3992 4941 Fragments arranged by size: 4941 3992 293 AclI AA'CG_TT Cuts at: 1563 7353 7726 1563 Size: 5790 373 3063 Fragments arranged by size: 5790 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2766 3618 3767 5342 6468 7289 8673 Size: 455 679 852 149 1575 1126 821 1384 Cuts at: 8673 8941 1632 Size: 268 1917 Fragments arranged by size: 1917 1575 1384 1126 852 821 679 455 268 149 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9029 AflIII A'CryG_T Cuts at: 1203 4571 4608 5608 8125 9102 1203 Size: 3368 37 1000 2517 977 1327 Fragments arranged by size: 3368 2517 1327 1000 977 37 AgeI A'CCGG_T Cuts at: 8057 8057 Size: 9226 AhdI GACnn_n'nnGTC Cuts at: 4609 7955 4609 Size: 3346 5880 AleI CACnn'nnGTG Cuts at: 1166 2681 4005 5867 6245 1166 Size: 1515 1324 1862 378 4147 Fragments arranged by size: 4147 1862 1515 1324 378 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6525 1184 Size: 5341 3885 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4271 4303 4742 4774 4271 Size: 32 439 32 8723 Fragments arranged by size: 8723 439 32 32 AlwNI CAG_nnn'CTG Cuts at: 6969 8541 6969 Size: 1572 7654 ApaI G_GGCC'C Cuts at: 5545 5545 Size: 9226 ApaLI G'TGCA_C Cuts at: 2750 7283 8439 2750 Size: 4533 1156 3537 Fragments arranged by size: 4533 3537 1156 ApoI r'AATT_y Cuts at: 1493 2114 2576 2620 3344 3882 4997 5375 5474 Size: 621 462 44 724 538 1115 378 99 Cuts at: 5474 5528 6356 6535 6761 6913 9022 1493 Size: 54 828 179 226 152 2109 1697 Fragments arranged by size: 2109 1697 1115 828 724 621 538 462 378 226 179 152 99 54 44 AseI AT'TA_AT Cuts at: 160 1032 7780 160 Size: 872 6748 1606 Fragments arranged by size: 6748 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9226 AvaI C'yCGr_G Cuts at: 1870 1943 3169 3279 5544 5632 6551 1870 Size: 73 1226 110 2265 88 919 4545 Fragments arranged by size: 4545 2265 1226 919 110 88 73 AvrII C'CTAG_G Cuts at: 4252 4252 Size: 9226 BamHI G'GATC_C Cuts at: 2605 6556 6923 2605 Size: 3951 367 4908 Fragments arranged by size: 4908 3951 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2262 2555 2939 5335 6547 Size: 325 217 627 475 293 384 2396 1212 Cuts at: 6547 8002 618 Size: 1455 1842 Fragments arranged by size: 2396 1842 1455 1212 627 475 384 325 293 217 BanII G_rGCy'C Cuts at: 1391 2344 4707 4794 5545 6545 1391 Size: 953 2363 87 751 1000 4072 Fragments arranged by size: 4072 2363 1000 953 751 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2075 3666 4301 5057 928 Size: 971 176 1591 635 756 5097 Fragments arranged by size: 5097 1591 971 756 635 176 BbvCI CC'TCA_GC Cuts at: 2631 3851 4817 5283 2631 Size: 1220 966 466 6574 Fragments arranged by size: 6574 1220 966 466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3711 3745 6446 6480 7417 Size: 34 50 34 1690 34 2701 34 937 Cuts at: 7417 7451 1903 Size: 34 3678 Fragments arranged by size: 3678 2701 1690 937 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3457 4297 5033 7118 8328 3457 Size: 840 736 2085 1210 4355 Fragments arranged by size: 4355 2085 1210 840 736 BclI T'GATC_A Cuts at: 1548 1548 Size: 9226 BfrBI ATG'CAT Cuts at: 3121 3121 Size: 9226 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4098 7837 136 Size: 107 122 71 631 605 2426 3739 1525 Fragments arranged by size: 3739 2426 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4537 9221 4537 Size: 4684 4542 BlpI GC'TnA_GC Cuts at: 6643 6643 Size: 9226 Bme1580I G_kGCm'C Cuts at: 1790 2754 5258 5545 7287 8443 1790 Size: 964 2504 287 1742 1156 2573 Fragments arranged by size: 2573 2504 1742 1156 964 287 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9226 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2378 3767 3809 Size: 416 53 83 399 24 961 1389 42 Cuts at: 3809 5507 6239 6957 7915 442 Size: 1698 732 718 958 1753 Fragments arranged by size: 1753 1698 1389 961 958 732 718 416 399 83 53 42 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9226 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 5308 5823 3617 Size: 1691 515 7020 Fragments arranged by size: 7020 1691 515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3360 6042 7886 1239 Size: 2121 2682 1844 2579 Fragments arranged by size: 2682 2579 2121 1844 Bpu10I CC'TnA_GC Cuts at: 2631 3814 3851 4817 5283 5331 2631 Size: 1183 37 966 466 48 6526 Fragments arranged by size: 6526 1183 966 466 48 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3348 3587 5818 6246 7351 8216 8514 3348 Size: 239 2231 428 1105 865 298 4060 Fragments arranged by size: 4060 2231 1105 865 428 298 239 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7889 882 Size: 617 6390 2219 Fragments arranged by size: 6390 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 7017 493 Size: 711 5813 2702 Fragments arranged by size: 5813 2702 711 BsaBI GATnn'nnATC Cuts at: 2804 6119 9220 2804 Size: 3315 3101 2810 Fragments arranged by size: 3315 3101 2810 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7033 7415 8050 275 Size: 53 83 186 936 5500 382 635 1451 Fragments arranged by size: 5500 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7659 8057 8331 8478 8889 7659 Size: 398 274 147 411 7996 Fragments arranged by size: 7996 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2733 2763 2879 2909 4742 Size: 30 165 30 1330 30 116 30 1833 Cuts at: 4742 4772 4805 4835 5871 5901 1178 Size: 30 33 30 1036 30 4503 Fragments arranged by size: 4503 1833 1330 1036 165 116 33 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2754 4457 6404 1186 Size: 1568 1703 1947 4008 Fragments arranged by size: 4008 1947 1703 1568 BseYI C'CCAG_C Cuts at: 2827 4350 6278 8429 8925 2827 Size: 1523 1928 2151 496 3128 Fragments arranged by size: 3128 2151 1928 1523 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2082 3167 6476 1163 Size: 573 346 1085 3309 3913 Fragments arranged by size: 3913 3309 1085 573 346 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6676 7437 8465 1540 Size: 275 186 4675 761 1028 2301 Fragments arranged by size: 4675 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2344 2754 3603 5090 5511 7287 7372 8443 2344 Size: 410 849 1487 421 1776 85 1071 3127 Fragments arranged by size: 3127 1776 1487 1071 849 421 410 85 BsmI GAATG_Cn' Cuts at: 2532 4118 4177 4229 6737 6830 2532 Size: 1586 59 52 2508 93 4928 Fragments arranged by size: 4928 2508 1586 93 59 52 BsmBI CGTCTCn'nnnn_ Cuts at: 4060 4508 6264 4060 Size: 448 1756 7022 Fragments arranged by size: 7022 1756 448 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2344 2754 3603 4707 4794 5090 5258 Size: 399 554 410 849 1104 87 296 168 Cuts at: 5258 5511 5545 6545 7287 7372 8443 1391 Size: 253 34 1000 742 85 1071 2174 Fragments arranged by size: 2174 1104 1071 1000 849 742 554 410 399 296 253 168 87 85 34 BspEI T'CCGG_A Cuts at: 8889 8889 Size: 9226 BspHI T'CATG_A Cuts at: 7115 8845 7115 Size: 1730 7496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5612 6581 844 Size: 4768 969 3489 Fragments arranged by size: 4768 3489 969 BsrBI CCG'CTC Cuts at: 1394 1823 7113 1394 Size: 429 5290 3507 Fragments arranged by size: 5290 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2483 3420 4233 4584 6078 7721 7895 Size: 1183 1235 937 813 351 1494 1643 174 Cuts at: 7895 65 Size: 1396 Fragments arranged by size: 1643 1494 1396 1235 1183 937 813 351 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7870 8057 1700 Size: 110 6060 187 2869 Fragments arranged by size: 6060 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9226 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9226 BssSI C'ACGA_G Cuts at: 7286 8298 7286 Size: 1012 8214 BstAPI GCAn_nnn'nTGC Cuts at: 794 3272 4235 4247 794 Size: 2478 963 12 5773 Fragments arranged by size: 5773 2478 963 12 BstBI TT'CG_AA Cuts at: 8030 8030 Size: 9226 BstEII G'GTnAC_C Cuts at: 1184 3163 4333 6430 1184 Size: 1979 1170 2097 3980 Fragments arranged by size: 3980 2097 1979 1170 BstXI CCAn_nnnn'nTGG Cuts at: 2683 3445 6247 6252 2683 Size: 762 2802 5 5657 Fragments arranged by size: 5657 2802 762 5 BstYI r'GATC_y Cuts at: 1972 2605 3359 4354 4537 6556 6923 7308 7325 Size: 633 754 995 183 2019 367 385 17 Cuts at: 7325 8777 8863 8875 9221 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2019 1977 1452 995 754 633 385 367 346 183 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2961 3990 4945 6195 513 Size: 552 1896 1029 955 1250 3544 Fragments arranged by size: 3544 1896 1250 1029 955 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8487 Fragments arranged by size: 8487 683 56 BtsI GCAGTG_nn' Cuts at: 3729 4996 5030 6742 7536 7556 3729 Size: 1267 34 1712 794 20 5399 Fragments arranged by size: 5399 1712 1267 794 34 20 ClaI AT'CG_AT Cuts at: 1554 3731 1554 Size: 2177 7049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5370 5405 529 Size: 35 4806 35 4350 Fragments arranged by size: 4806 4350 35 35 DraI TTT'AAA Cuts at: 4680 6530 4680 Size: 1850 7376 DraIII CAC_nnn'GTG Cuts at: 3803 5515 3803 Size: 1712 7514 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3956 8233 809 Size: 507 2640 4277 1802 Fragments arranged by size: 4277 2640 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 2313 2964 Size: 54 1425 50 275 18 379 104 651 Cuts at: 2964 3553 4523 5859 6137 6254 6673 7562 9191 Size: 589 970 1336 278 117 419 889 1629 Cuts at: 9191 8 Size: 43 Fragments arranged by size: 1629 1425 1336 970 889 651 589 419 379 278 275 117 104 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6673 1537 Size: 275 4861 4090 Fragments arranged by size: 4861 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5118 5478 6292 7156 1353 Size: 3765 360 814 864 3423 Fragments arranged by size: 3765 3423 864 814 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2711 3778 7813 8187 8333 387 Size: 1064 138 1122 1067 4035 374 146 1280 Fragments arranged by size: 4035 1280 1122 1067 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2342 2342 Size: 9226 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2766 3360 3618 3767 5342 6042 Size: 393 455 679 594 258 149 1575 700 Cuts at: 6042 6468 7289 7886 8673 8941 1239 Size: 426 821 597 787 268 1524 Fragments arranged by size: 1575 1524 821 787 700 679 597 594 455 426 393 268 258 149 EcoNI CCTnn'n_nnAGG Cuts at: 5483 5483 Size: 9226 EcoO109I rG'GnC_Cy Cuts at: 5541 5541 Size: 9226 EcoRI G'AATT_C Cuts at: 6535 6913 6535 Size: 378 8848 FspI TGC'GCA Cuts at: 7732 7732 Size: 9226 HaeII r_GCGC'y Cuts at: 1678 8373 1678 Size: 6695 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 2289 2321 4595 Size: 32 135 32 543 32 142 32 2274 Cuts at: 4595 4627 7867 7899 7941 7973 1373 Size: 32 3240 32 42 32 2626 Fragments arranged by size: 3240 2626 2274 543 142 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3094 5039 5845 6570 6816 669 Size: 988 281 1156 1945 806 725 246 3079 Fragments arranged by size: 3079 1945 1156 988 806 725 281 246 HindIII A'AGCT_T Cuts at: 748 4458 748 Size: 3710 5516 HpaI GTT'AAC Cuts at: 6816 6816 Size: 9226 KpnI G_GTAC'C Cuts at: 2266 2559 6551 2266 Size: 293 3992 4941 Fragments arranged by size: 4941 3992 293 MfeI C'AATT_G Cuts at: 3952 6825 3952 Size: 2873 6353 MluI A'CGCG_T Cuts at: 4571 9102 4571 Size: 4531 4695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3890 5173 5930 8340 8524 1305 Size: 2585 1283 757 2410 184 2007 Fragments arranged by size: 2585 2410 2007 1283 757 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2211 2315 2966 3555 4525 Size: 54 1425 343 379 104 651 589 970 Cuts at: 4525 5861 10 Size: 1336 3375 Fragments arranged by size: 3375 1425 1336 970 651 589 379 343 104 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 2681 2783 3039 4005 4166 5648 Size: 648 1359 156 102 256 966 161 1482 Cuts at: 5648 5855 5867 6245 7184 7543 7702 518 Size: 207 12 378 939 359 159 2042 Fragments arranged by size: 2042 1482 1359 966 939 648 378 359 256 207 161 159 156 102 12 MspA1I CmG'CkG Cuts at: 1606 3670 4350 5306 6392 6642 7319 8467 8712 Size: 2064 680 956 1086 250 677 1148 245 Cuts at: 8712 1606 Size: 2120 Fragments arranged by size: 2120 2064 1148 1086 956 680 677 250 245 NcoI C'CATG_G Cuts at: 513 1065 2961 3990 4945 513 Size: 552 1896 1029 955 4794 Fragments arranged by size: 4794 1896 1029 955 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9226 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9226 NotI GC'GGCC_GC Cuts at: 6673 6673 Size: 9226 NsiI A_TGCA'T Cuts at: 3123 3123 Size: 9226 NspI r_CATG'y Cuts at: 1132 1229 4612 5172 5612 6584 8129 1132 Size: 97 3383 560 440 972 1545 2229 Fragments arranged by size: 3383 2229 1545 972 560 440 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9226 PciI A'CATG_T Cuts at: 4608 5608 8125 4608 Size: 1000 2517 5709 Fragments arranged by size: 5709 2517 1000 PflMI CCAn_nnn'nTGG Cuts at: 1108 2455 2897 3886 1108 Size: 1347 442 989 6448 Fragments arranged by size: 6448 1347 989 442 PfoI T'CCnGG_A Cuts at: 2172 4289 2172 Size: 2117 7109 PmeI GTTT'AAAC Cuts at: 6530 6530 Size: 9226 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4742 4774 7295 7327 8808 8840 4742 Size: 32 2521 32 1481 32 5128 Fragments arranged by size: 5128 2521 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9226 PsiI TTA'TAA Cuts at: 6796 6796 Size: 9226 PspOMI G'GGCC_C Cuts at: 5541 5541 Size: 9226 PstI C_TGCA'G Cuts at: 830 4873 5271 6578 830 Size: 4043 398 1307 3478 Fragments arranged by size: 4043 3478 1307 398 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9226 PvuII CAG'CTG Cuts at: 3670 5306 6392 3670 Size: 1636 1086 6504 Fragments arranged by size: 6504 1636 1086 SacI G_AGCT'C Cuts at: 2344 2344 Size: 9226 SalI G'TCGA_C Cuts at: 1936 6568 1936 Size: 4632 4594 SbfI CC_TGCA'GG Cuts at: 6578 6578 Size: 9226 ScaI AGT'ACT Cuts at: 7474 9186 7474 Size: 1712 7514 SfcI C'TryA_G Cuts at: 826 1045 2483 2691 4482 4869 5267 6496 6574 Size: 219 1438 208 1791 387 398 1229 78 Cuts at: 6574 7709 8390 8581 826 Size: 1135 681 191 1471 Fragments arranged by size: 1791 1471 1438 1229 1135 681 398 387 219 208 191 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9226 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9226 SmaI CCC'GGG Cuts at: 1872 3171 6553 1872 Size: 1299 3382 4545 Fragments arranged by size: 4545 3382 1299 SmlI C'TyrA_G Cuts at: 820 1017 1943 3363 3602 5797 6261 7330 8231 Size: 197 926 1420 239 2195 464 1069 901 Cuts at: 8231 8493 820 Size: 262 1553 Fragments arranged by size: 2195 1553 1420 1069 926 901 464 262 239 197 SnaBI TAC'GTA Cuts at: 493 7017 493 Size: 6524 2702 SpeI A'CTAG_T Cuts at: 152 3268 152 Size: 3116 6110 SphI G_CATG'C Cuts at: 1132 6584 1132 Size: 5452 3774 SspI AAT'ATT Cuts at: 5 52 2384 7150 5 Size: 47 2332 4766 2081 Fragments arranged by size: 4766 2332 2081 47 StuI AGG'CCT Cuts at: 1517 3863 5413 1517 Size: 2346 1550 5330 Fragments arranged by size: 5330 2346 1550 StyI C'CwwG_G Cuts at: 513 1065 2643 2961 3045 3990 4252 4590 4786 Size: 552 1578 318 84 945 262 338 196 Cuts at: 4786 4841 4945 5133 5339 5674 5865 6148 6665 Size: 55 104 188 206 335 191 283 517 Cuts at: 6665 513 Size: 3074 Fragments arranged by size: 3074 1578 945 552 517 338 335 318 283 262 206 196 191 188 104 84 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3171 3539 3791 3983 4097 4491 5526 Size: 1255 1591 368 252 192 114 394 1035 Cuts at: 5526 7253 7270 7423 7608 8053 325 Size: 1727 17 153 185 445 1498 Fragments arranged by size: 1727 1591 1498 1255 1035 445 394 368 252 192 185 153 114 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2906 5115 6504 Size: 274 80 33 51 1443 929 2209 1389 Cuts at: 6504 7472 9184 96 Size: 968 1712 138 Fragments arranged by size: 2209 1712 1443 1389 968 929 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2154 2186 2733 2765 4327 4359 4941 4973 9186 Size: 32 547 32 1562 32 582 32 4213 Cuts at: 9186 9218 2154 Size: 32 2162 Fragments arranged by size: 4213 2162 1562 582 547 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9226 XbaI T'CTAG_A Cuts at: 6562 6562 Size: 9226 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3883 6025 3883 Size: 2142 7084 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9226 XmaI C'CCGG_G Cuts at: 1870 3169 6551 1870 Size: 1299 3382 4545 Fragments arranged by size: 4545 3382 1299 XmnI GAAnn'nnTTC Cuts at: 2496 3933 5474 6048 7355 2496 Size: 1437 1541 574 1307 4367 Fragments arranged by size: 4367 1541 1437 1307 574 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7034 276 Size: 53 83 186 936 5500 2468 Fragments arranged by size: 5500 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI