################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI Aor51HI ApoI AscI AseI AsnI AspI AviII AvrII BalI BbeI BbrPI BciVI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DraIII EarI Eco47III EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI Ksp632I KspI MaeI MfeI MluI MluNI MroI MscI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tsp509I Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 492 GGTACC G^GTACC AcyI 1587 GACGCC G[GA]^CG[CT]C AflIII 1817 ACATGT A^C[GA][CT]GT AhdI 1186 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1303 GTGCAC G^TGCAC ApaLI 1303 GTGCAC G^TGCAC Asp700I 1257 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 492 GGTACC G^GTACC AspEI 1186 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1530 GGATCC G^GATCC BbvCI 1807 CCTCAGC CCTCAGC(-5/-2) BcgI 596 GCAATGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1275 TGATCA T^GATCA BsaBI 1096 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1587 GACGCC G[GA]^CG[CT]C BsaI 1361 GAGACC GGTCTC(1/5) BsiEI 413 CGGCCG CG[GA][CT]^CG BspLU11I 1817 ACATGT A^CATGT BssSI 928 CACGAG CACGAG(-5/-1) BstEII 1558 GGTGACC G^GT[ACGT]ACC BstPI 1558 GGTGACC G^GT[ACGT]ACC DrdI 1627 GACATGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 413 CGGCCG C^GGCCG Eam1105I 1186 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 413 CGGCCG C^GGCCG Eco52I 413 CGGCCG C^GGCCG EcoNI 855 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1558 GGTGACC G^GT[ACGT]ACC FbaI 1275 TGATCA T^GATCA Hin1I 1587 GACGCC G[GA]^CG[CT]C HincII 1891 GTTGAC GT[CT]^[GA]AC HindII 1891 GTTGAC GT[CT]^[GA]AC KpnI 492 GGTACC GGTAC^C MamI 1096 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 1109 TTAA T^TAA MspA1I 958 CAGCTG C[AC]G^C[GT]G NaeI 607 GCCGGC GCC^GGC NgoMIV 607 GCCGGC G^CCGGC PaeR7I 1828 CTCGAG C^TCGAG PshAI 241 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 958 CAGCTG CAG^CTG SacI 1542 GAGCTC GAGCT^C SexAI 50 ACCAGGT A^CC[AT]GGT SmlI 1828 CTCGAG C^T[CT][GA]AG SphI 614 GCATGC GCATG^C StuI 1270 AGGCCT AGG^CCT Tru9I 1109 TTAA T^TAA XhoI 1828 CTCGAG C^TCGAG XmnI 1257 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 484 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG 1491 CAGTATCTG AocI 855 CCTGAGG CC^T[ACGT]AGG 1885 CCTGAGG BsgI 79 CTGCAC GTGCAG(16/14) 650 GTGCAG BsmAI 1361 GAGAC GTCTC(1/5) 1821 GTCTC BspHI 407 TCATGA T^CATGA 1389 TCATGA BstF5I 764 CATCC GGATG(2/0) 1731 CATCC Bsu36I 855 CCTGAGG CC^T[ACGT]AGG 1885 CCTGAGG EaeI 176 TGGCCG [CT]^GGCC[GA] 413 CGGCCG Eco81I 855 CCTGAGG CC^T[ACGT]AGG 1885 CCTGAGG FokI 764 CATCC GGATG(9/13) 1731 CATCC HaeII 113 GGCGCT [GA]GCGC^[CT] 211 AGCGCC MaeII 125 ACGT A^CGT 479 ACGT MslI 69 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 375 CATCGTGGTG NcoI 707 CCATGG C^CATGG 1904 CCATGG PstI 183 CTGCAG CTGCA^G 1488 CTGCAG RcaI 407 TCATGA T^CATGA 1389 TCATGA ScaI 191 AGTACT AGT^ACT 1424 AGTACT SfcI 183 CTGCAG C^T[GA][CT]AG 1488 CTGCAG Tsp45I 946 GTCAC ^GT[GC]AC 1559 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AfaI AluI AlwI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BglI BglII BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI TspRI TthHB8I XcmI XhoII