(Circular) (Six-base) MAPSORT of: pFN21AB3020.seq Check: 2172 from: 1 to: 6628 pFN21AB3020 6628 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3055 3295 3055 Size: 240 6388 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4438 278 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3971 1937 Size: 2034 4594 Acc65I G'GTAC_C Cuts at: 2497 3949 2497 Size: 1452 5176 AclI AA'CG_TT Cuts at: 1563 4755 5128 1563 Size: 3192 373 3063 Fragments arranged by size: 3192 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3042 3482 3608 3939 4691 6075 6343 1632 Size: 1410 440 126 331 752 1384 268 1917 Fragments arranged by size: 1917 1410 1384 752 440 331 268 126 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6431 AflIII A'CryG_T Cuts at: 1203 3822 5527 6504 1203 Size: 2619 1705 977 1327 Fragments arranged by size: 2619 1705 1327 977 AgeI A'CCGG_T Cuts at: 5459 5459 Size: 6628 AhdI GACnn_n'nnGTC Cuts at: 3196 5357 3196 Size: 2161 4467 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6628 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2096 2402 1184 Size: 912 306 5410 Fragments arranged by size: 5410 912 306 AlwNI CAG_nnn'CTG Cuts at: 2494 3501 4371 5943 2494 Size: 1007 870 1572 3179 Fragments arranged by size: 3179 1572 1007 870 ApaI G_GGCC'C Cuts at: 3483 3770 3788 3483 Size: 287 18 6323 Fragments arranged by size: 6323 287 18 ApaLI G'TGCA_C Cuts at: 3308 4685 5841 3308 Size: 1377 1156 4095 Fragments arranged by size: 4095 1377 1156 ApoI r'AATT_y Cuts at: 1493 3937 4163 4315 6424 1493 Size: 2444 226 152 2109 1697 Fragments arranged by size: 2444 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5182 160 Size: 872 4150 1606 Fragments arranged by size: 4150 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 AvaI C'yCGr_G Cuts at: 1870 1943 2643 3363 3761 3833 3953 1870 Size: 73 700 720 398 72 120 4545 Fragments arranged by size: 4545 720 700 398 120 73 72 BamHI G'GATC_C Cuts at: 3535 3958 4325 3535 Size: 423 367 5838 Fragments arranged by size: 5838 423 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2497 2873 3406 3949 5404 Size: 325 217 627 710 376 533 543 1455 Cuts at: 5404 618 Size: 1842 Fragments arranged by size: 1842 1455 710 627 543 533 376 325 217 BanII G_rGCy'C Cuts at: 1391 3483 3551 3760 3770 3788 3947 1391 Size: 2092 68 209 10 18 159 4072 Fragments arranged by size: 4072 2092 209 159 68 18 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3030 3405 3596 928 Size: 971 1131 375 191 3960 Fragments arranged by size: 3960 1131 971 375 191 BbvCI CC'TCA_GC Cuts at: 3813 3813 Size: 6628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2590 2624 4819 4853 1903 Size: 34 653 34 2195 34 3678 Fragments arranged by size: 3678 2195 653 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4520 5730 4520 Size: 1210 5418 BclI T'GATC_A Cuts at: 1548 3280 1548 Size: 1732 4896 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 2180 3913 Size: 107 122 71 631 605 368 140 1733 Cuts at: 3913 5239 136 Size: 1326 1525 Fragments arranged by size: 1733 1525 1326 631 605 368 140 122 107 71 BglII A'GATC_T Cuts at: 2454 3078 3090 6623 2454 Size: 624 12 3533 2459 Fragments arranged by size: 3533 2459 624 12 BlpI GC'TnA_GC Cuts at: 4045 4045 Size: 6628 Bme1580I G_kGCm'C Cuts at: 1790 2779 3312 3411 3483 3770 3788 4689 5845 Size: 989 533 99 72 287 18 901 1156 Cuts at: 5845 1790 Size: 2573 Fragments arranged by size: 2573 1156 989 901 533 287 99 72 18 BmgBI CAC'GTC Cuts at: 1318 2131 1318 Size: 813 5815 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2215 2754 4359 Size: 416 53 83 399 24 798 539 1605 Cuts at: 4359 5317 442 Size: 958 1753 Fragments arranged by size: 1753 1605 958 798 539 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6628 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2945 3256 5288 1239 Size: 1097 609 311 2032 2579 Fragments arranged by size: 2579 2032 1097 609 311 Bpu10I CC'TnA_GC Cuts at: 3813 3813 Size: 6628 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4753 5618 5916 4753 Size: 865 298 5465 Fragments arranged by size: 5465 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3360 5291 882 Size: 617 1861 1931 2219 Fragments arranged by size: 2219 1931 1861 617 BsaAI yAC'GTr Cuts at: 493 1204 4419 493 Size: 711 3215 2702 Fragments arranged by size: 3215 2702 711 BsaBI GATnn'nnATC Cuts at: 3105 6622 3105 Size: 3517 3111 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3593 4435 4817 5452 Size: 53 83 186 936 2060 842 382 635 Cuts at: 5452 275 Size: 1451 Fragments arranged by size: 2060 1451 936 842 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5061 5459 5733 5880 6291 5061 Size: 398 274 147 411 5398 Fragments arranged by size: 5398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3662 3692 1178 Size: 30 165 30 2259 30 4114 Fragments arranged by size: 4114 2259 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2645 2648 2976 3061 3801 3821 1186 Size: 1459 3 328 85 740 20 3993 Fragments arranged by size: 3993 1459 740 328 85 20 3 BseYI C'CCAG_C Cuts at: 2161 2940 2965 2995 3482 5831 6327 2161 Size: 779 25 30 487 2349 496 2462 Fragments arranged by size: 2462 2349 779 496 487 30 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 2676 1163 Size: 573 333 607 5115 Fragments arranged by size: 5115 607 573 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2421 4078 4839 5867 1540 Size: 275 186 420 1657 761 1028 2301 Fragments arranged by size: 2301 1657 1028 761 420 275 186 BsiHKAI G_wGCw'C Cuts at: 2136 3312 3551 4689 4774 5845 2136 Size: 1176 239 1138 85 1071 2919 Fragments arranged by size: 2919 1176 1138 1071 239 85 BsmI GAATG_Cn' Cuts at: 4139 4232 4139 Size: 93 6535 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2779 3312 3411 3483 3551 3760 Size: 399 346 643 533 99 72 68 209 Cuts at: 3760 3770 3788 3947 4689 4774 5845 1391 Size: 10 18 159 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 643 533 399 346 209 159 99 85 72 68 18 10 BspEI T'CCGG_A Cuts at: 6291 6291 Size: 6628 BspHI T'CATG_A Cuts at: 2412 3394 4517 6247 2412 Size: 982 1123 1730 2793 Fragments arranged by size: 2793 1730 1123 982 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2835 3055 3295 3350 3983 844 Size: 1991 220 240 55 633 3489 Fragments arranged by size: 3489 1991 633 240 220 55 BsrBI CCG'CTC Cuts at: 1394 1823 2011 3322 3711 4515 1394 Size: 429 188 1311 389 804 3507 Fragments arranged by size: 3507 1311 804 429 389 188 BsrDI GCAATG_nn' Cuts at: 65 1248 2608 2661 3352 5123 5297 65 Size: 1183 1360 53 691 1771 174 1396 Fragments arranged by size: 1771 1396 1360 1183 691 174 53 BsrFI r'CCGG_y Cuts at: 1700 1810 2612 2919 3117 5272 5459 1700 Size: 110 802 307 198 2155 187 2869 Fragments arranged by size: 2869 2155 802 307 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6628 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6628 BssSI C'ACGA_G Cuts at: 2933 4688 5700 2933 Size: 1755 1012 3861 Fragments arranged by size: 3861 1755 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2663 2774 3492 794 Size: 1869 111 718 3930 Fragments arranged by size: 3930 1869 718 111 BstBI TT'CG_AA Cuts at: 5432 5432 Size: 6628 BstEII G'GTnAC_C Cuts at: 1184 3563 1184 Size: 2379 4249 BstXI CCAn_nnnn'nTGG Cuts at: 2830 3257 3422 2830 Size: 427 165 6036 Fragments arranged by size: 6036 427 165 BstYI r'GATC_y Cuts at: 1972 2454 2490 3078 3090 3535 3886 3958 4325 Size: 482 36 588 12 445 351 72 367 Cuts at: 4325 4710 4727 6179 6265 6277 6623 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 588 482 445 385 367 351 346 86 72 36 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2861 3891 2861 Size: 1030 5598 BtgI C'CryG_G Cuts at: 513 1065 2004 2712 3228 3909 513 Size: 552 939 708 516 681 3232 Fragments arranged by size: 3232 939 708 681 552 516 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2593 487 Size: 683 56 1367 4522 Fragments arranged by size: 4522 1367 683 56 BtsI GCAGTG_nn' Cuts at: 4144 4938 4958 4144 Size: 794 20 5814 Fragments arranged by size: 5814 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6628 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6593 DraI TTT'AAA Cuts at: 3932 3932 Size: 6628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3638 5635 809 Size: 507 2322 1997 1802 Fragments arranged by size: 2322 1997 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2418 4075 Size: 54 1425 50 275 18 351 237 1657 Cuts at: 4075 4964 6593 8 Size: 889 1629 43 Fragments arranged by size: 1657 1629 1425 889 351 275 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2418 4075 1537 Size: 275 606 1657 4090 Fragments arranged by size: 4090 1657 606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4558 1353 Size: 3205 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2250 5215 5589 5735 387 Size: 1064 138 661 2965 374 146 1280 Fragments arranged by size: 2965 1280 1064 661 374 146 138 EcoICRI GAG'CTC Cuts at: 3549 3549 Size: 6628 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2945 3042 3256 3482 3608 3939 Size: 393 704 609 97 214 226 126 331 Cuts at: 3939 4691 5288 6075 6343 1239 Size: 752 597 787 268 1524 Fragments arranged by size: 1524 787 752 704 609 597 393 331 268 226 214 126 97 EcoNI CCTnn'n_nnAGG Cuts at: 2864 2864 Size: 6628 EcoO109I rG'GnC_Cy Cuts at: 2541 2847 2857 3479 3766 3767 3778 3785 3914 Size: 306 10 622 287 1 11 7 129 Cuts at: 3914 2541 Size: 5255 Fragments arranged by size: 5255 622 306 287 129 11 10 7 1 EcoRI G'AATT_C Cuts at: 3937 4315 3937 Size: 378 6250 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3739 3739 Size: 6628 FspI TGC'GCA Cuts at: 5134 5134 Size: 6628 HaeII r_GCGC'y Cuts at: 1678 2122 2220 5775 1678 Size: 444 98 3555 2531 Fragments arranged by size: 3555 2531 444 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3182 3214 5269 5301 5343 Size: 32 135 32 1610 32 2055 32 42 Cuts at: 5343 5375 1373 Size: 32 2626 Fragments arranged by size: 2626 2055 1610 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3898 3972 4218 669 Size: 988 281 1960 74 246 3079 Fragments arranged by size: 3079 1960 988 281 246 74 HindIII A'AGCT_T Cuts at: 748 748 Size: 6628 HpaI GTT'AAC Cuts at: 4218 4218 Size: 6628 KpnI G_GTAC'C Cuts at: 2501 3953 2501 Size: 1452 5176 MfeI C'AATT_G Cuts at: 4227 4227 Size: 6628 MluI A'CGCG_T Cuts at: 6504 6504 Size: 6628 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2505 5742 5926 1305 Size: 1200 3237 184 2007 Fragments arranged by size: 3237 2007 1200 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4806 Fragments arranged by size: 4806 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 2384 4586 4945 5104 518 Size: 648 912 306 2202 359 159 2042 Fragments arranged by size: 2202 2042 912 648 359 306 159 MspA1I CmG'CkG Cuts at: 1606 2965 4044 4721 5869 6114 1606 Size: 1359 1079 677 1148 245 2120 Fragments arranged by size: 2120 1359 1148 1079 677 245 NaeI GCC'GGC Cuts at: 2614 2614 Size: 6628 NcoI C'CATG_G Cuts at: 513 1065 2004 2712 3909 513 Size: 552 939 708 1197 3232 Fragments arranged by size: 3232 1197 939 708 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6628 NgoMIV G'CCGG_C Cuts at: 2612 2612 Size: 6628 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6628 NotI GC'GGCC_GC Cuts at: 4075 4075 Size: 6628 NspI r_CATG'y Cuts at: 1132 1229 2623 3199 3752 3826 3986 5531 1132 Size: 97 1394 576 553 74 160 1545 2229 Fragments arranged by size: 2229 1545 1394 576 553 160 97 74 PasI CC'CwG_GG Cuts at: 1348 2537 2852 3772 1348 Size: 1189 315 920 4204 Fragments arranged by size: 4204 1189 920 315 PciI A'CATG_T Cuts at: 3822 5527 3822 Size: 1705 4923 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6628 PmeI GTTT'AAAC Cuts at: 3932 3932 Size: 6628 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2195 2227 4697 4729 6210 6242 2195 Size: 32 2470 32 1481 32 2581 Fragments arranged by size: 2581 2470 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2541 2847 3778 2541 Size: 306 931 5391 Fragments arranged by size: 5391 931 306 PshAI GACnn'nnGTC Cuts at: 1613 2250 1613 Size: 637 5991 PsiI TTA'TAA Cuts at: 4198 4198 Size: 6628 PspOMI G'GGCC_C Cuts at: 3479 3766 3784 3479 Size: 287 18 6323 Fragments arranged by size: 6323 287 18 PspXI vC'TCGA_Gb Cuts at: 3833 3833 Size: 6628 PstI C_TGCA'G Cuts at: 830 2192 3497 3980 830 Size: 1362 1305 483 3478 Fragments arranged by size: 3478 1362 1305 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6628 PvuII CAG'CTG Cuts at: 2965 2965 Size: 6628 SacI G_AGCT'C Cuts at: 3551 3551 Size: 6628 SalI G'TCGA_C Cuts at: 1936 3970 1936 Size: 2034 4594 SanDI GG'GwC_CC Cuts at: 2847 2847 Size: 6628 SbfI CC_TGCA'GG Cuts at: 3980 3980 Size: 6628 ScaI AGT'ACT Cuts at: 2198 3431 4876 6588 2198 Size: 1233 1445 1712 2238 Fragments arranged by size: 2238 1712 1445 1233 SexAI A'CCwGG_T Cuts at: 2055 2055 Size: 6628 SfcI C'TryA_G Cuts at: 826 1045 2188 3493 3976 5111 5792 5983 826 Size: 219 1143 1305 483 1135 681 191 1471 Fragments arranged by size: 1471 1305 1143 1135 681 483 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6628 SmaI CCC'GGG Cuts at: 1872 3955 1872 Size: 2083 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3833 4732 5633 5895 820 Size: 197 926 1890 899 901 262 1553 Fragments arranged by size: 1890 1553 926 901 899 262 197 SnaBI TAC'GTA Cuts at: 493 4419 493 Size: 3926 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6628 SphI G_CATG'C Cuts at: 1132 2623 3986 1132 Size: 1491 1363 3774 Fragments arranged by size: 3774 1491 1363 SspI AAT'ATT Cuts at: 5 52 4552 5 Size: 47 4500 2081 Fragments arranged by size: 4500 2081 47 StuI AGG'CCT Cuts at: 1517 3277 1517 Size: 1760 4868 StyI C'CwwG_G Cuts at: 513 1065 2004 2337 2586 2712 2813 3793 3909 Size: 552 939 333 249 126 101 980 116 Cuts at: 3909 4067 513 Size: 158 3074 Fragments arranged by size: 3074 980 939 552 333 249 158 126 116 101 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2987 4655 4672 4825 5010 5455 325 Size: 1255 1407 1668 17 153 185 445 1498 Fragments arranged by size: 1668 1498 1407 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2196 3429 4874 Size: 274 80 33 51 1443 219 1233 1445 Cuts at: 4874 6586 96 Size: 1712 138 Fragments arranged by size: 1712 1445 1443 1233 274 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3113 3145 6588 6620 3113 Size: 32 3443 32 3121 Fragments arranged by size: 3443 3121 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6628 XbaI T'CTAG_A Cuts at: 3964 3964 Size: 6628 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2962 3064 3652 2962 Size: 102 588 5938 Fragments arranged by size: 5938 588 102 XhoI C'TCGA_G Cuts at: 1943 3833 1943 Size: 1890 4738 XmaI C'CCGG_G Cuts at: 1870 3953 1870 Size: 2083 4545 XmnI GAAnn'nnTTC Cuts at: 3266 4757 3266 Size: 1491 5137 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4436 276 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI