Restriction map for
pFN21AB3020
(Linear) (Six-base) MAPSORT of: KIBB3020.seq Check: 5176 from: 1 to: 1923
KIBB3020.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1050 1290 1923
Size: 1050 240 633
Fragments arranged by size:
1050 633 240
Acc65I G'GTAC_C
Cuts at: 0 492 1923
Size: 492 1431
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1477 1603 1923
Size: 1037 440 126 320
Fragments arranged by size:
1037 440 320 126
AflIII A'CryG_T
Cuts at: 0 1817 1923
Size: 1817 106
AhdI GACnn_n'nnGTC
Cuts at: 0 1191 1923
Size: 1191 732
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 91 397 1923
Size: 91 306 1526
Fragments arranged by size:
1526 306 91
AlwNI CAG_nnn'CTG
Cuts at: 0 489 1496 1923
Size: 489 1007 427
Fragments arranged by size:
1007 489 427
ApaI G_GGCC'C
Cuts at: 0 1478 1765 1783 1923
Size: 1478 287 18 140
Fragments arranged by size:
1478 287 140 18
ApaLI G'TGCA_C
Cuts at: 0 1303 1923
Size: 1303 620
AvaI C'yCGr_G
Cuts at: 0 638 1358 1756 1828 1923
Size: 638 720 398 72 95
Fragments arranged by size:
720 638 398 95 72
BamHI G'GATC_C
Cuts at: 0 1530 1923
Size: 1530 393
BanI G'GyrC_C
Cuts at: 0 492 868 1401 1923
Size: 492 376 533 522
Fragments arranged by size:
533 522 492 376
BanII G_rGCy'C
Cuts at: 0 1478 1546 1755 1765 1783 1923
Size: 1478 68 209 10 18 140
Fragments arranged by size:
1478 209 140 68 18 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 1025 1400 1591 1923
Size: 1025 375 191 332
Fragments arranged by size:
1025 375 332 191
BbvCI CC'TCA_GC
Cuts at: 0 1808 1923
Size: 1808 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 585 619 1923
Size: 585 34 1304
Fragments arranged by size:
1304 585 34
BclI T'GATC_A
Cuts at: 0 1275 1923
Size: 1275 648
BglI GCCn_nnn'nGGC
Cuts at: 0 35 175 1908 1923
Size: 35 140 1733 15
Fragments arranged by size:
1733 140 35 15
BglII A'GATC_T
Cuts at: 0 449 1073 1085 1923
Size: 449 624 12 838
Fragments arranged by size:
838 624 449 12
Bme1580I G_kGCm'C
Cuts at: 0 774 1307 1406 1478 1765 1783 1923
Size: 774 533 99 72 287 18 140
Fragments arranged by size:
774 533 287 140 99 72 18
BmgBI CAC'GTC
Cuts at: 0 126 1923
Size: 126 1797
BmrI ACTGGGnnnn_n'
Cuts at: 0 210 749 1923
Size: 210 539 1174
Fragments arranged by size:
1174 539 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 940 1251 1923
Size: 331 609 311 672
Fragments arranged by size:
672 609 331 311
Bpu10I CC'TnA_GC
Cuts at: 0 1808 1923
Size: 1808 115
BsaI GGTCTCn'nnnn_
Cuts at: 0 1355 1923
Size: 1355 568
BsaBI GATnn'nnATC
Cuts at: 0 1100 1923
Size: 1100 823
BsaHI Gr'CG_yC
Cuts at: 0 1588 1923
Size: 1588 335
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1657 1687 1923
Size: 1657 30 236
Fragments arranged by size:
1657 236 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 640 643 971 1056 1796 1816 1923
Size: 640 3 328 85 740 20 107
Fragments arranged by size:
740 640 328 107 85 20 3
BseYI C'CCAG_C
Cuts at: 0 156 935 960 990 1477 1923
Size: 156 779 25 30 487 446
Fragments arranged by size:
779 487 446 156 30 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 671 1923
Size: 64 607 1252
Fragments arranged by size:
1252 607 64
BsiEI CG_ry'CG
Cuts at: 0 416 1923
Size: 416 1507
BsiHKAI G_wGCw'C
Cuts at: 0 131 1307 1546 1923
Size: 131 1176 239 377
Fragments arranged by size:
1176 377 239 131
Bsp1286I G_dGCh'C
Cuts at: 0 131 774 1307 1406 1478 1546 1755 1765
Size: 131 643 533 99 72 68 209 10
Cuts at: 1765 1783 1923
Size: 18 140
Fragments arranged by size:
643 533 209 140 131 99 72 68
18 10
BspHI T'CATG_A
Cuts at: 0 407 1389 1923
Size: 407 982 534
Fragments arranged by size:
982 534 407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 830 1050 1290 1345 1923
Size: 830 220 240 55 578
Fragments arranged by size:
830 578 240 220 55
BsrBI CCG'CTC
Cuts at: 0 6 1317 1706 1923
Size: 6 1311 389 217
Fragments arranged by size:
1311 389 217 6
BsrDI GCAATG_nn'
Cuts at: 0 603 656 1347 1923
Size: 603 53 691 576
Fragments arranged by size:
691 603 576 53
BsrFI r'CCGG_y
Cuts at: 0 607 914 1112 1923
Size: 607 307 198 811
Fragments arranged by size:
811 607 307 198
BssSI C'ACGA_G
Cuts at: 0 928 1923
Size: 928 995
BstAPI GCAn_nnn'nTGC
Cuts at: 0 658 769 1487 1923
Size: 658 111 718 436
Fragments arranged by size:
718 658 436 111
BstEII G'GTnAC_C
Cuts at: 0 1558 1923
Size: 1558 365
BstXI CCAn_nnnn'nTGG
Cuts at: 0 825 1252 1417 1923
Size: 825 427 165 506
Fragments arranged by size:
825 506 427 165
BstYI r'GATC_y
Cuts at: 0 449 485 1073 1085 1530 1881 1923
Size: 449 36 588 12 445 351 42
Fragments arranged by size:
588 449 445 351 42 36 12
Bsu36I CC'TnA_GG
Cuts at: 0 856 1886 1923
Size: 856 1030 37
Fragments arranged by size:
1030 856 37
BtgI C'CryG_G
Cuts at: 0 707 1223 1904 1923
Size: 707 516 681 19
Fragments arranged by size:
707 681 516 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 588 1923
Size: 588 1335
DrdI GACnn_nn'nnGTC
Cuts at: 0 1633 1923
Size: 1633 290
EaeI y'GGCC_r
Cuts at: 0 176 413 1923
Size: 176 237 1510
Fragments arranged by size:
1510 237 176
EagI C'GGCC_G
Cuts at: 0 413 1923
Size: 413 1510
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 245 1923
Size: 245 1678
EcoICRI GAG'CTC
Cuts at: 0 1544 1923
Size: 1544 379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 940 1037 1251 1477 1603 1923
Size: 331 609 97 214 226 126 320
Fragments arranged by size:
609 331 320 226 214 126 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 859 1923
Size: 859 1064
EcoO109I rG'GnC_Cy
Cuts at: 0 536 842 852 1474 1761 1762 1773 1780
Size: 536 306 10 622 287 1 11 7
Cuts at: 1780 1909 1923
Size: 129 14
Fragments arranged by size:
622 536 306 287 129 14 11 10
7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1734 1923
Size: 1734 189
HaeII r_GCGC'y
Cuts at: 0 117 215 1923
Size: 117 98 1708
Fragments arranged by size:
1708 117 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1177 1209 1923
Size: 1177 32 714
Fragments arranged by size:
1177 714 32
HincII GTy'rAC
Cuts at: 0 1893 1923
Size: 1893 30
KpnI G_GTAC'C
Cuts at: 0 496 1923
Size: 496 1427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 1923
Size: 500 1423
MslI CAynn'nnrTG
Cuts at: 0 73 379 1923
Size: 73 306 1544
Fragments arranged by size:
1544 306 73
MspA1I CmG'CkG
Cuts at: 0 960 1923
Size: 960 963
NaeI GCC'GGC
Cuts at: 0 609 1923
Size: 609 1314
NcoI C'CATG_G
Cuts at: 0 707 1904 1923
Size: 707 1197 19
Fragments arranged by size:
1197 707 19
NgoMIV G'CCGG_C
Cuts at: 0 607 1923
Size: 607 1316
NspI r_CATG'y
Cuts at: 0 618 1194 1747 1821 1923
Size: 618 576 553 74 102
Fragments arranged by size:
618 576 553 102 74
PasI CC'CwG_GG
Cuts at: 0 532 847 1767 1923
Size: 532 315 920 156
Fragments arranged by size:
920 532 315 156
PciI A'CATG_T
Cuts at: 0 1817 1923
Size: 1817 106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 1923
Size: 190 32 1701
Fragments arranged by size:
1701 190 32
PpuMI rG'GwC_Cy
Cuts at: 0 536 842 1773 1923
Size: 536 306 931 150
Fragments arranged by size:
931 536 306 150
PshAI GACnn'nnGTC
Cuts at: 0 245 1923
Size: 245 1678
PspOMI G'GGCC_C
Cuts at: 0 1474 1761 1779 1923
Size: 1474 287 18 144
Fragments arranged by size:
1474 287 144 18
PspXI vC'TCGA_Gb
Cuts at: 0 1828 1923
Size: 1828 95
PstI C_TGCA'G
Cuts at: 0 187 1492 1923
Size: 187 1305 431
Fragments arranged by size:
1305 431 187
PvuII CAG'CTG
Cuts at: 0 960 1923
Size: 960 963
SacI G_AGCT'C
Cuts at: 0 1546 1923
Size: 1546 377
SanDI GG'GwC_CC
Cuts at: 0 842 1923
Size: 842 1081
ScaI AGT'ACT
Cuts at: 0 193 1426 1923
Size: 193 1233 497
Fragments arranged by size:
1233 497 193
SexAI A'CCwGG_T
Cuts at: 0 50 1923
Size: 50 1873
SfcI C'TryA_G
Cuts at: 0 183 1488 1923
Size: 183 1305 435
Fragments arranged by size:
1305 435 183
SmlI C'TyrA_G
Cuts at: 0 1828 1923
Size: 1828 95
SphI G_CATG'C
Cuts at: 0 618 1923
Size: 618 1305
StuI AGG'CCT
Cuts at: 0 1272 1923
Size: 1272 651
StyI C'CwwG_G
Cuts at: 0 332 581 707 808 1788 1904 1923
Size: 332 249 126 101 980 116 19
Fragments arranged by size:
980 332 249 126 116 101 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 982 1923
Size: 982 941
TatI w'GTAC_w
Cuts at: 0 191 1424 1923
Size: 191 1233 499
Fragments arranged by size:
1233 499 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1108 1140 1923
Size: 1108 32 783
Fragments arranged by size:
1108 783 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 957 1059 1647 1923
Size: 957 102 588 276
Fragments arranged by size:
957 588 276 102
XhoI C'TCGA_G
Cuts at: 0 1828 1923
Size: 1828 95
XmnI GAAnn'nnTTC
Cuts at: 0 1261 1923
Size: 1261 662
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI DrdI
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII KpnI MmeI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PciI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SanDI ScaI SexAI
SfcI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI
BfrBI BlpI BmtI BplI BpuEI BsaAI BsaWI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstBI BstZ17I BtsI
ClaI CspCI DraI DraIII EarI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI MfeI MluI MscI
NarI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PmlI PsiI PsrI PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmaI
ZraI