(Circular) (Six-base) MAPSORT of: pFN21AB3016.seq Check: 9542 from: 1 to: 7042 pFN21AB3016 7042 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4224 4852 278 Size: 53 83 186 936 2688 628 2468 Fragments arranged by size: 2688 2468 936 628 186 83 53 AccI GT'mk_AC Cuts at: 1937 4041 4385 1937 Size: 2104 344 4594 Fragments arranged by size: 4594 2104 344 Acc65I G'GTAC_C Cuts at: 4363 4363 Size: 7042 AclI AA'CG_TT Cuts at: 1563 3920 5169 5542 1563 Size: 2357 1249 373 3063 Fragments arranged by size: 3063 2357 1249 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2382 3192 5105 6489 6757 1632 Size: 750 810 1913 1384 268 1917 Fragments arranged by size: 1917 1913 1384 810 750 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6845 AflIII A'CryG_T Cuts at: 1203 3491 5941 6918 1203 Size: 2288 2450 977 1327 Fragments arranged by size: 2450 2288 1327 977 AgeI A'CCGG_T Cuts at: 5873 5873 Size: 7042 AhdI GACnn_n'nnGTC Cuts at: 5771 5771 Size: 7042 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7042 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7042 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2388 2420 3936 3968 2388 Size: 32 1516 32 5462 Fragments arranged by size: 5462 1516 32 32 AlwNI CAG_nnn'CTG Cuts at: 2897 4785 6357 2897 Size: 1888 1572 3582 Fragments arranged by size: 3582 1888 1572 ApaLI G'TGCA_C Cuts at: 5099 6255 5099 Size: 1156 5886 ApoI r'AATT_y Cuts at: 1493 2501 2723 4106 4351 4577 4729 6838 1493 Size: 1008 222 1383 245 226 152 2109 1697 Fragments arranged by size: 2109 1697 1383 1008 245 226 222 152 AseI AT'TA_AT Cuts at: 160 1032 5596 160 Size: 872 4564 1606 Fragments arranged by size: 4564 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7042 AvaI C'yCGr_G Cuts at: 1870 1943 4367 1870 Size: 73 2424 4545 Fragments arranged by size: 4545 2424 73 BamHI G'GATC_C Cuts at: 2384 3361 4372 4739 2384 Size: 977 1011 367 4687 Fragments arranged by size: 4687 1011 977 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 3720 4363 5818 618 Size: 325 217 627 248 1685 643 1455 1842 Fragments arranged by size: 1842 1685 1455 643 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 3709 4279 4361 1391 Size: 2318 570 82 4072 Fragments arranged by size: 4072 2318 570 82 BbeI G_GCGC'C Cuts at: 2039 2039 Size: 7042 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3565 928 Size: 971 1666 4405 Fragments arranged by size: 4405 1666 971 BbvCI CC'TCA_GC Cuts at: 3970 3970 Size: 7042 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5233 5267 1903 Size: 34 3296 34 3678 Fragments arranged by size: 3678 3296 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2390 4934 6144 2390 Size: 2544 1210 3288 Fragments arranged by size: 3288 2544 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7042 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2044 3195 5653 Size: 107 122 71 631 605 372 1151 2458 Cuts at: 5653 136 Size: 1525 Fragments arranged by size: 2458 1525 1151 631 605 372 122 107 71 BglII A'GATC_T Cuts at: 3529 3905 7037 3529 Size: 376 3132 3534 Fragments arranged by size: 3534 3132 376 BlpI GC'TnA_GC Cuts at: 4459 4459 Size: 7042 Bme1580I G_kGCm'C Cuts at: 1790 5103 6259 1790 Size: 3313 1156 2573 Fragments arranged by size: 3313 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2133 4142 1318 Size: 815 2009 4218 Fragments arranged by size: 4218 2009 815 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3944 4773 5731 Size: 416 53 83 399 24 2527 829 958 Cuts at: 5731 442 Size: 1753 Fragments arranged by size: 2527 1753 958 829 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7042 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2107 2107 Size: 7042 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2841 3164 3666 3693 5702 1239 Size: 821 781 323 502 27 2009 2579 Fragments arranged by size: 2579 2009 821 781 502 323 27 Bpu10I CC'TnA_GC Cuts at: 3970 3970 Size: 7042 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4307 5167 6032 6330 4307 Size: 860 865 298 5019 Fragments arranged by size: 5019 865 860 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2064 2478 3824 5705 882 Size: 617 565 414 1346 1881 2219 Fragments arranged by size: 2219 1881 1346 617 565 414 BsaAI yAC'GTr Cuts at: 493 1204 4833 493 Size: 711 3629 2702 Fragments arranged by size: 3629 2702 711 BsaBI GATnn'nnATC Cuts at: 7036 7036 Size: 7042 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 4221 4849 5231 Size: 53 83 186 936 503 2185 628 382 Cuts at: 5231 5866 275 Size: 635 1451 Fragments arranged by size: 2185 1451 936 635 628 503 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5475 5873 6147 6294 6705 5475 Size: 398 274 147 411 5812 Fragments arranged by size: 5812 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6817 Fragments arranged by size: 6817 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 3001 1186 Size: 880 935 5227 Fragments arranged by size: 5227 935 880 BseYI C'CCAG_C Cuts at: 6245 6741 6245 Size: 496 6546 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3516 1163 Size: 573 1780 4689 Fragments arranged by size: 4689 1780 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4492 5253 6281 1540 Size: 275 186 2491 761 1028 2301 Fragments arranged by size: 2491 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3709 5103 5188 6259 3709 Size: 1394 85 1071 4492 Fragments arranged by size: 4492 1394 1071 85 BsmI GAATG_Cn' Cuts at: 2663 4553 4646 2663 Size: 1890 93 5059 Fragments arranged by size: 5059 1890 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3709 4279 4361 5103 5188 6259 1391 Size: 399 1919 570 82 742 85 1071 2174 Fragments arranged by size: 2174 1919 1071 742 570 399 85 82 BspEI T'CCGG_A Cuts at: 6705 6705 Size: 7042 BspHI T'CATG_A Cuts at: 3979 4015 4931 6661 3979 Size: 36 916 1730 4360 Fragments arranged by size: 4360 1730 916 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4397 844 Size: 3553 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2062 2086 4929 1394 Size: 429 239 24 2843 3507 Fragments arranged by size: 3507 2843 429 239 24 BsrDI GCAATG_nn' Cuts at: 65 1248 3558 5537 5711 65 Size: 1183 2310 1979 174 1396 Fragments arranged by size: 2310 1979 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5686 5873 1700 Size: 110 3876 187 2869 Fragments arranged by size: 3876 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7042 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7042 BssSI C'ACGA_G Cuts at: 2745 5102 6114 2745 Size: 2357 1012 3673 Fragments arranged by size: 3673 2357 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7042 BstBI TT'CG_AA Cuts at: 5846 5846 Size: 7042 BstEII G'GTnAC_C Cuts at: 1184 4027 1184 Size: 2843 4199 BstXI CCAn_nnnn'nTGG Cuts at: 3717 3717 Size: 7042 BstYI r'GATC_y Cuts at: 1972 2384 3225 3361 3529 3905 4372 4739 5124 Size: 412 841 136 168 376 467 367 385 Cuts at: 5124 5141 6593 6679 6691 7037 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 841 467 412 385 376 367 346 168 136 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2793 513 Size: 552 1728 4762 Fragments arranged by size: 4762 1728 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2089 487 Size: 683 56 863 5440 Fragments arranged by size: 5440 863 683 56 BtsI GCAGTG_nn' Cuts at: 4135 4558 5352 5372 4135 Size: 423 794 20 5805 Fragments arranged by size: 5805 794 423 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7042 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3682 3717 529 Size: 35 3118 35 3854 Fragments arranged by size: 3854 3118 35 35 DraI TTT'AAA Cuts at: 2616 4346 2616 Size: 1730 5312 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6049 809 Size: 507 4733 1802 Fragments arranged by size: 4733 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2845 3187 3433 Size: 54 1425 50 275 18 1015 342 246 Cuts at: 3433 4489 5378 7007 8 Size: 1056 889 1629 43 Fragments arranged by size: 1629 1425 1056 1015 889 342 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4489 1537 Size: 275 2677 4090 Fragments arranged by size: 4090 2677 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2112 4972 1353 Size: 723 36 2860 3423 Fragments arranged by size: 3423 2860 723 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5629 6003 6149 387 Size: 1064 138 4040 374 146 1280 Fragments arranged by size: 4040 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3707 3707 Size: 7042 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2382 2841 3164 3192 3666 3693 Size: 393 428 322 459 323 28 474 27 Cuts at: 3693 5105 5702 6489 6757 1239 Size: 1412 597 787 268 1524 Fragments arranged by size: 1524 1412 787 597 474 459 428 393 323 322 268 28 27 EcoO109I rG'GnC_Cy Cuts at: 3842 4305 3842 Size: 463 6579 EcoRI G'AATT_C Cuts at: 4351 4729 4351 Size: 378 6664 EcoRV GAT'ATC Cuts at: 2554 2800 2554 Size: 246 6796 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3797 3797 Size: 7042 FspI TGC'GCA Cuts at: 5548 5548 Size: 7042 HaeII r_GCGC'y Cuts at: 1678 2039 6189 1678 Size: 361 4150 2531 Fragments arranged by size: 4150 2531 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2962 2994 3777 3809 4196 Size: 32 135 32 1390 32 783 32 387 Cuts at: 4196 4228 4241 4273 5683 5715 5757 5789 1373 Size: 32 13 32 1410 32 42 32 2626 Fragments arranged by size: 2626 1410 1390 783 387 135 42 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 4386 4632 669 Size: 988 281 2448 246 3079 Fragments arranged by size: 3079 2448 988 281 246 HindIII A'AGCT_T Cuts at: 748 3800 748 Size: 3052 3990 HpaI GTT'AAC Cuts at: 4632 4632 Size: 7042 KasI G'GCGC_C Cuts at: 2035 2035 Size: 7042 KpnI G_GTAC'C Cuts at: 4367 4367 Size: 7042 MfeI C'AATT_G Cuts at: 4641 4641 Size: 7042 MluI A'CGCG_T Cuts at: 6918 6918 Size: 7042 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2180 3114 6156 6340 1305 Size: 875 934 3042 184 2007 Fragments arranged by size: 3042 2007 934 875 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2847 3435 10 Size: 54 1425 343 1015 588 3617 Fragments arranged by size: 3617 1425 1015 588 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2808 5000 5359 5518 518 Size: 648 1642 2192 359 159 2042 Fragments arranged by size: 2192 2042 1642 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4458 5135 6283 6528 1606 Size: 2852 677 1148 245 2120 Fragments arranged by size: 2852 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2036 2036 Size: 7042 NcoI C'CATG_G Cuts at: 513 1065 2793 513 Size: 552 1728 4762 Fragments arranged by size: 4762 1728 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7042 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7042 NotI GC'GGCC_GC Cuts at: 4489 4489 Size: 7042 NspI r_CATG'y Cuts at: 1132 1229 3495 4400 5945 1132 Size: 97 2266 905 1545 2229 Fragments arranged by size: 2266 2229 1545 905 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7042 PciI A'CATG_T Cuts at: 3491 5941 3491 Size: 2450 4592 PflMI CCAn_nnn'nTGG Cuts at: 1108 3011 1108 Size: 1903 5139 PfoI T'CCnGG_A Cuts at: 2076 3141 2076 Size: 1065 5977 PmeI GTTT'AAAC Cuts at: 4346 4346 Size: 7042 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5111 5143 6624 6656 5111 Size: 32 1481 32 5497 Fragments arranged by size: 5497 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4305 4305 Size: 7042 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7042 PsiI TTA'TAA Cuts at: 4612 4612 Size: 7042 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2530 2562 2530 Size: 32 7010 PstI C_TGCA'G Cuts at: 830 4394 830 Size: 3564 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7042 SacI G_AGCT'C Cuts at: 3709 3709 Size: 7042 SalI G'TCGA_C Cuts at: 1936 4384 1936 Size: 2448 4594 SapI GCTCTTCn'nnn_ Cuts at: 2076 2076 Size: 7042 SbfI CC_TGCA'GG Cuts at: 4394 4394 Size: 7042 ScaI AGT'ACT Cuts at: 3858 5290 7002 3858 Size: 1432 1712 3898 Fragments arranged by size: 3898 1712 1432 SfcI C'TryA_G Cuts at: 826 1045 2547 3229 3303 4390 5525 6206 6397 Size: 219 1502 682 74 1087 1135 681 191 Cuts at: 6397 826 Size: 1471 Fragments arranged by size: 1502 1471 1135 1087 682 681 219 191 74 SfoI GGC'GCC Cuts at: 2037 2037 Size: 7042 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7042 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7042 SmaI CCC'GGG Cuts at: 1872 4369 1872 Size: 2497 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4286 5146 6047 6309 820 Size: 197 926 2343 860 901 262 1553 Fragments arranged by size: 2343 1553 926 901 860 262 197 SnaBI TAC'GTA Cuts at: 493 4833 493 Size: 4340 2702 SpeI A'CTAG_T Cuts at: 152 3290 152 Size: 3138 3904 SphI G_CATG'C Cuts at: 1132 4400 1132 Size: 3268 3774 SspI AAT'ATT Cuts at: 5 52 2685 2949 3043 4966 5 Size: 47 2633 264 94 1923 2081 Fragments arranged by size: 2633 2081 1923 264 94 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7042 StyI C'CwwG_G Cuts at: 513 1065 2039 2793 4481 513 Size: 552 974 754 1688 3074 Fragments arranged by size: 3074 1688 974 754 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5069 5086 5239 5424 5869 325 Size: 1255 3489 17 153 185 445 1498 Fragments arranged by size: 3489 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3856 5288 7000 Size: 274 80 33 51 1443 1879 1432 1712 Cuts at: 7000 96 Size: 138 Fragments arranged by size: 1879 1712 1443 1432 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7002 7034 7002 Size: 32 7010 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7042 XbaI T'CTAG_A Cuts at: 4378 4378 Size: 7042 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3130 3130 Size: 7042 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7042 XmaI C'CCGG_G Cuts at: 1870 4367 1870 Size: 2497 4545 XmnI GAAnn'nnTTC Cuts at: 5171 5171 Size: 7042 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4222 4850 276 Size: 53 83 186 936 2688 628 2468 Fragments arranged by size: 2688 2468 936 628 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI