(Circular) (Six-base) MAPSORT of: pFN21AB3015.seq Check: 2697 from: 1 to: 6913 pFN21AB3015 6913 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2815 3265 2815 Size: 450 6463 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3072 4723 278 Size: 53 83 186 936 1536 1651 2468 Fragments arranged by size: 2468 1651 1536 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3748 4256 1937 Size: 1811 508 4594 Fragments arranged by size: 4594 1811 508 Acc65I G'GTAC_C Cuts at: 4234 4234 Size: 6913 AclI AA'CG_TT Cuts at: 1563 5040 5413 1563 Size: 3477 373 3063 Fragments arranged by size: 3477 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2889 3432 4976 6360 6628 1632 Size: 1257 543 1544 1384 268 1917 Fragments arranged by size: 1917 1544 1384 1257 543 268 AfeI AGC'GCT Cuts at: 3722 3722 Size: 6913 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6716 AflIII A'CryG_T Cuts at: 1203 5812 6789 1203 Size: 4609 977 1327 Fragments arranged by size: 4609 1327 977 AgeI A'CCGG_T Cuts at: 2148 5744 2148 Size: 3596 3317 AhdI GACnn_n'nnGTC Cuts at: 5642 5642 Size: 6913 AleI CACnn'nnGTG Cuts at: 1166 3035 1166 Size: 1869 5044 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6913 AlwNI CAG_nnn'CTG Cuts at: 2380 4656 6228 2380 Size: 2276 1572 3065 Fragments arranged by size: 3065 2276 1572 ApaLI G'TGCA_C Cuts at: 4970 6126 4970 Size: 1156 5757 ApoI r'AATT_y Cuts at: 1493 2384 2519 2690 3010 4222 4448 4600 6709 Size: 891 135 171 320 1212 226 152 2109 Cuts at: 6709 1493 Size: 1697 Fragments arranged by size: 2109 1697 1212 891 320 226 171 152 135 AseI AT'TA_AT Cuts at: 160 1032 5467 160 Size: 872 4435 1606 Fragments arranged by size: 4435 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 AvaI C'yCGr_G Cuts at: 1870 1943 4238 1870 Size: 73 2295 4545 Fragments arranged by size: 4545 2295 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3054 3087 3054 Size: 33 6880 BamHI G'GATC_C Cuts at: 4243 4610 4243 Size: 367 6546 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3125 4234 5689 618 Size: 325 217 627 1338 1109 1455 1842 Fragments arranged by size: 1842 1455 1338 1109 627 325 217 BanII G_rGCy'C Cuts at: 1391 3341 4232 1391 Size: 1950 891 4072 Fragments arranged by size: 4072 1950 891 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5942 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5104 5138 1903 Size: 34 3167 34 3678 Fragments arranged by size: 3678 3167 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3686 4805 6015 3686 Size: 1119 1210 4584 Fragments arranged by size: 4584 1210 1119 BclI T'GATC_A Cuts at: 1548 1548 Size: 6913 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5524 136 Size: 107 122 71 631 605 3852 1525 Fragments arranged by size: 3852 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3285 6908 3285 Size: 3623 3290 BlpI GC'TnA_GC Cuts at: 4330 4330 Size: 6913 Bme1580I G_kGCm'C Cuts at: 1790 2903 3128 4974 6130 1790 Size: 1113 225 1846 1156 2573 Fragments arranged by size: 2573 1846 1156 1113 225 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6913 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2736 4644 5602 Size: 416 53 83 399 24 1319 1908 958 Cuts at: 5602 442 Size: 1753 Fragments arranged by size: 1908 1753 1319 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3229 3642 4017 5573 1239 Size: 1990 413 375 1556 2579 Fragments arranged by size: 2579 1990 1556 413 375 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2170 5038 5903 6201 2170 Size: 2868 865 298 2882 Fragments arranged by size: 2882 2868 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5576 882 Size: 617 4077 2219 Fragments arranged by size: 4077 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4704 493 Size: 711 3500 2702 Fragments arranged by size: 3500 2702 711 BsaBI GATnn'nnATC Cuts at: 6907 6907 Size: 6913 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3069 4720 5102 5737 Size: 53 83 186 936 1536 1651 382 635 Cuts at: 5737 275 Size: 1451 Fragments arranged by size: 1651 1536 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2148 5346 5744 6018 6165 6576 2148 Size: 3198 398 274 147 411 2485 Fragments arranged by size: 3198 2485 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3217 3247 3785 3815 1178 Size: 30 165 30 1814 30 538 30 4276 Fragments arranged by size: 4276 1814 538 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2538 2586 2814 1186 Size: 1352 48 228 5285 Fragments arranged by size: 5285 1352 228 48 BseYI C'CCAG_C Cuts at: 3465 4098 4130 6116 6612 3465 Size: 633 32 1986 496 3766 Fragments arranged by size: 3766 1986 633 496 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3699 1163 Size: 573 1963 4377 Fragments arranged by size: 4377 1963 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4363 5124 6152 1540 Size: 275 186 2362 761 1028 2301 Fragments arranged by size: 2362 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4974 5059 6130 4974 Size: 85 1071 5757 Fragments arranged by size: 5757 1071 85 BsmI GAATG_Cn' Cuts at: 2426 2795 4424 4517 2426 Size: 369 1629 93 4822 Fragments arranged by size: 4822 1629 369 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3501 3512 3501 Size: 11 6902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2903 3128 3341 4232 4974 5059 6130 Size: 399 1113 225 213 891 742 85 1071 Cuts at: 6130 1391 Size: 2174 Fragments arranged by size: 2174 1113 1071 891 742 399 225 213 85 BspEI T'CCGG_A Cuts at: 6576 6576 Size: 6913 BspHI T'CATG_A Cuts at: 4802 6532 4802 Size: 1730 5183 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2556 2815 3265 4268 844 Size: 1712 259 450 1003 3489 Fragments arranged by size: 3489 1712 1003 450 259 BsrBI CCG'CTC Cuts at: 1394 1823 4800 1394 Size: 429 2977 3507 Fragments arranged by size: 3507 2977 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2865 5408 5582 65 Size: 1183 1617 2543 174 1396 Fragments arranged by size: 2543 1617 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2148 4026 5557 5744 1700 Size: 110 338 1878 1531 187 2869 Fragments arranged by size: 2869 1878 1531 338 187 110 BsrGI T'GTAC_A Cuts at: 96 2347 96 Size: 2251 4662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6913 BssSI C'ACGA_G Cuts at: 4973 5985 4973 Size: 1012 5901 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6913 BstBI TT'CG_AA Cuts at: 3688 5717 3688 Size: 2029 4884 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6913 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2207 2122 Size: 85 6828 BstYI r'GATC_y Cuts at: 1972 2494 2884 3285 4164 4243 4610 4995 5012 Size: 522 390 401 879 79 367 385 17 Cuts at: 5012 6464 6550 6562 6908 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 879 522 401 390 385 367 346 86 79 17 12 BstZ17I GTA'TAC Cuts at: 3749 3749 Size: 6913 BtgI C'CryG_G Cuts at: 513 1065 3311 513 Size: 552 2246 4115 Fragments arranged by size: 4115 2246 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2121 487 Size: 683 56 895 5279 Fragments arranged by size: 5279 895 683 56 BtsI GCAGTG_nn' Cuts at: 2280 2403 4044 4429 5223 5243 2280 Size: 123 1641 385 794 20 3950 Fragments arranged by size: 3950 1641 794 385 123 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6913 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6878 DraI TTT'AAA Cuts at: 4217 4217 Size: 6913 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2626 5920 809 Size: 507 1310 3294 1802 Fragments arranged by size: 3294 1802 1310 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3264 4360 5249 Size: 54 1425 50 275 18 1434 1096 889 Cuts at: 5249 6878 8 Size: 1629 43 Fragments arranged by size: 1629 1434 1425 1096 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4360 1537 Size: 275 2548 4090 Fragments arranged by size: 4090 2548 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2229 2232 4843 1353 Size: 876 3 2611 3423 Fragments arranged by size: 3423 2611 876 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5500 5874 6020 387 Size: 1064 138 3911 374 146 1280 Fragments arranged by size: 3911 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2889 3229 3432 3642 4017 4976 5573 Size: 393 1257 340 203 210 375 959 597 Cuts at: 5573 6360 6628 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1257 959 787 597 393 375 340 268 210 203 EcoNI CCTnn'n_nnAGG Cuts at: 3057 3057 Size: 6913 EcoRI G'AATT_C Cuts at: 4222 4600 4222 Size: 378 6535 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3814 3814 Size: 6913 FspI TGC'GCA Cuts at: 5419 5419 Size: 6913 HaeII r_GCGC'y Cuts at: 1678 3724 6060 1678 Size: 2046 2336 2531 Fragments arranged by size: 2531 2336 2046 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3527 3559 3614 3646 5554 Size: 32 135 32 1955 32 55 32 1908 Cuts at: 5554 5586 5628 5660 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1955 1908 135 55 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3042 3382 3828 4257 4503 669 Size: 988 281 1104 340 446 429 246 3079 Fragments arranged by size: 3079 1104 988 446 429 340 281 246 HindIII A'AGCT_T Cuts at: 748 3650 748 Size: 2902 4011 HpaI GTT'AAC Cuts at: 3042 4503 3042 Size: 1461 5452 KpnI G_GTAC'C Cuts at: 4238 4238 Size: 6913 MfeI C'AATT_G Cuts at: 4512 4512 Size: 6913 MluI A'CGCG_T Cuts at: 6789 6789 Size: 6913 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3141 6027 6211 1305 Size: 1836 2886 184 2007 Fragments arranged by size: 2886 2007 1836 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3266 10 Size: 54 1425 343 1434 3657 Fragments arranged by size: 3657 1434 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2988 3035 4871 5230 5389 518 Size: 648 954 868 47 1836 359 159 2042 Fragments arranged by size: 2042 1836 954 868 648 359 159 47 MspA1I CmG'CkG Cuts at: 1606 2086 2134 3667 3932 4329 5006 6154 6399 Size: 480 48 1533 265 397 677 1148 245 Cuts at: 6399 1606 Size: 2120 Fragments arranged by size: 2120 1533 1148 677 480 397 265 245 48 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6361 NdeI CA'TA_TG Cuts at: 387 2623 2989 3484 387 Size: 2236 366 495 3816 Fragments arranged by size: 3816 2236 495 366 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6913 NotI GC'GGCC_GC Cuts at: 4360 4360 Size: 6913 NspI r_CATG'y Cuts at: 1132 1229 2169 4271 5816 1132 Size: 97 940 2102 1545 2229 Fragments arranged by size: 2229 2102 1545 940 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6913 PciI A'CATG_T Cuts at: 5812 5812 Size: 6913 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6913 PfoI T'CCnGG_A Cuts at: 2805 2805 Size: 6913 PmeI GTTT'AAAC Cuts at: 4217 4217 Size: 6913 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4982 5014 6495 6527 4982 Size: 32 1481 32 5368 Fragments arranged by size: 5368 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6913 PsiI TTA'TAA Cuts at: 3395 4483 3395 Size: 1088 5825 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3984 4016 3984 Size: 32 6881 PstI C_TGCA'G Cuts at: 830 2410 3937 4265 830 Size: 1580 1527 328 3478 Fragments arranged by size: 3478 1580 1527 328 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6913 PvuII CAG'CTG Cuts at: 3667 3932 3667 Size: 265 6648 RsrII CG'GwC_CG Cuts at: 2151 2151 Size: 6913 SalI G'TCGA_C Cuts at: 1936 4255 1936 Size: 2319 4594 SbfI CC_TGCA'GG Cuts at: 4265 4265 Size: 6913 ScaI AGT'ACT Cuts at: 3400 3409 3926 3979 5161 6873 3400 Size: 9 517 53 1182 1712 3440 Fragments arranged by size: 3440 1712 1182 517 53 9 SexAI A'CCwGG_T Cuts at: 3346 3346 Size: 6913 SfcI C'TryA_G Cuts at: 826 1045 2406 3933 4261 5396 6077 6268 826 Size: 219 1361 1527 328 1135 681 191 1471 Fragments arranged by size: 1527 1471 1361 1135 681 328 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6913 SmaI CCC'GGG Cuts at: 1872 4240 1872 Size: 2368 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2185 5017 5918 6180 820 Size: 197 926 242 2832 901 262 1553 Fragments arranged by size: 2832 1553 926 901 262 242 197 SnaBI TAC'GTA Cuts at: 493 4704 493 Size: 4211 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6913 SphI G_CATG'C Cuts at: 1132 2169 4271 1132 Size: 1037 2102 3774 Fragments arranged by size: 3774 2102 1037 SspI AAT'ATT Cuts at: 5 52 2356 4837 5 Size: 47 2304 2481 2081 Fragments arranged by size: 2481 2304 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6913 StyI C'CwwG_G Cuts at: 513 1065 4352 513 Size: 552 3287 3074 Fragments arranged by size: 3287 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4940 4957 5110 5295 5740 325 Size: 1255 3360 17 153 185 445 1498 Fragments arranged by size: 3360 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2347 3398 3407 Size: 274 80 33 51 1443 370 1051 9 Cuts at: 3407 3924 3977 4199 5159 6871 96 Size: 517 53 222 960 1712 138 Fragments arranged by size: 1712 1443 1051 960 517 370 274 222 138 80 53 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2306 2338 2927 2959 3217 3249 6873 6905 2306 Size: 32 589 32 258 32 3624 32 2314 Fragments arranged by size: 3624 2314 589 258 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2717 1316 Size: 1401 5512 XbaI T'CTAG_A Cuts at: 2497 4249 2497 Size: 1752 5161 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2702 2702 Size: 6913 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6913 XmaI C'CCGG_G Cuts at: 1870 4238 1870 Size: 2368 4545 XmnI GAAnn'nnTTC Cuts at: 5042 5042 Size: 6913 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3070 4721 276 Size: 53 83 186 936 1536 1651 2468 Fragments arranged by size: 2468 1651 1536 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII RsrII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI SacI SacII SanDI SapI SfiI SfoI SrfI SwaI