(Circular) (Six-base) MAPSORT of: pFN21AB3011.seq Check: 6054 from: 1 to: 6868 pFN21AB3011 6868 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2693 2693 Size: 6868 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4678 278 Size: 53 83 186 936 3142 2468 Fragments arranged by size: 3142 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2118 4211 1937 Size: 181 2093 4594 Fragments arranged by size: 4594 2093 181 Acc65I G'GTAC_C Cuts at: 4189 4189 Size: 6868 AclI AA'CG_TT Cuts at: 1563 4167 4995 5368 1563 Size: 2604 828 373 3063 Fragments arranged by size: 3063 2604 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 2592 4931 6315 6583 1632 Size: 893 67 2339 1384 268 1917 Fragments arranged by size: 2339 1917 1384 893 268 67 AfeI AGC'GCT Cuts at: 3522 3522 Size: 6868 AflII C'TTAA_G Cuts at: 820 1017 4073 820 Size: 197 3056 3615 Fragments arranged by size: 3615 3056 197 AflIII A'CryG_T Cuts at: 1203 2646 5767 6744 1203 Size: 1443 3121 977 1327 Fragments arranged by size: 3121 1443 1327 977 AgeI A'CCGG_T Cuts at: 5699 5699 Size: 6868 AhdI GACnn_n'nnGTC Cuts at: 5597 5597 Size: 6868 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6868 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6868 AlwNI CAG_nnn'CTG Cuts at: 3544 4611 6183 3544 Size: 1067 1572 4229 Fragments arranged by size: 4229 1572 1067 ApaI G_GGCC'C Cuts at: 2372 2372 Size: 6868 ApaLI G'TGCA_C Cuts at: 2706 4925 6081 2706 Size: 2219 1156 3493 Fragments arranged by size: 3493 2219 1156 ApoI r'AATT_y Cuts at: 1493 2015 2889 4125 4177 4403 4555 6664 1493 Size: 522 874 1236 52 226 152 2109 1697 Fragments arranged by size: 2109 1697 1236 874 522 226 152 52 AseI AT'TA_AT Cuts at: 160 1032 2484 5422 160 Size: 872 1452 2938 1606 Fragments arranged by size: 2938 1606 1452 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6868 AvaI C'yCGr_G Cuts at: 1870 1943 4025 4193 1870 Size: 73 2082 168 4545 Fragments arranged by size: 4545 2082 168 73 BamHI G'GATC_C Cuts at: 2539 3238 4198 4565 2539 Size: 699 960 367 4842 Fragments arranged by size: 4842 960 699 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4189 5644 618 Size: 325 217 627 2402 1455 1842 Fragments arranged by size: 2402 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2294 2372 2424 3157 4187 1391 Size: 903 78 52 733 1030 4072 Fragments arranged by size: 4072 1030 903 733 78 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2221 2499 3768 928 Size: 971 322 278 1269 4028 Fragments arranged by size: 4028 1269 971 322 278 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5059 5093 1903 Size: 34 3122 34 3678 Fragments arranged by size: 3678 3122 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4760 5970 4760 Size: 1210 5658 BclI T'GATC_A Cuts at: 1548 1548 Size: 6868 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3277 5479 136 Size: 107 122 71 631 605 1605 2202 1525 Fragments arranged by size: 2202 1605 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2458 2597 3427 6863 2458 Size: 139 830 3436 2463 Fragments arranged by size: 3436 2463 830 139 BlpI GC'TnA_GC Cuts at: 4285 4285 Size: 6868 Bme1580I G_kGCm'C Cuts at: 1790 2372 2710 4929 6085 1790 Size: 582 338 2219 1156 2573 Fragments arranged by size: 2573 2219 1156 582 338 BmgBI CAC'GTC Cuts at: 1318 2606 1318 Size: 1288 5580 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2256 3073 4599 Size: 416 53 83 399 24 839 817 1526 Cuts at: 4599 5557 442 Size: 958 1753 Fragments arranged by size: 1753 1526 958 839 817 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6868 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 3254 3338 5528 1239 Size: 1142 873 84 2190 2579 Fragments arranged by size: 2579 2190 1142 873 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2408 4993 5858 6156 2408 Size: 2585 865 298 3120 Fragments arranged by size: 3120 2585 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5531 882 Size: 617 4032 2219 Fragments arranged by size: 4032 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2195 4659 493 Size: 711 991 2464 2702 Fragments arranged by size: 2702 2464 991 711 BsaBI GATnn'nnATC Cuts at: 3216 6862 3216 Size: 3646 3222 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4675 5057 5692 275 Size: 53 83 186 936 3142 382 635 1451 Fragments arranged by size: 3142 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5301 5699 5973 6120 6531 5301 Size: 398 274 147 411 5638 Fragments arranged by size: 5638 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3373 3403 1178 Size: 30 165 30 1970 30 4643 Fragments arranged by size: 4643 1970 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6868 BseYI C'CCAG_C Cuts at: 3621 6071 6567 3621 Size: 2450 496 3922 Fragments arranged by size: 3922 2450 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 1163 Size: 573 699 5596 Fragments arranged by size: 5596 699 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4318 5079 6107 1540 Size: 275 186 2317 761 1028 2301 Fragments arranged by size: 2317 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2710 3157 4929 5014 6085 2710 Size: 447 1772 85 1071 3493 Fragments arranged by size: 3493 1772 1071 447 85 BsmI GAATG_Cn' Cuts at: 4149 4379 4472 4149 Size: 230 93 6545 Fragments arranged by size: 6545 230 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2294 2372 2424 2710 3157 4187 4929 Size: 399 504 78 52 286 447 1030 742 Cuts at: 4929 5014 6085 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1030 742 504 447 399 286 85 78 52 BspEI T'CCGG_A Cuts at: 6531 6531 Size: 6868 BspHI T'CATG_A Cuts at: 2028 3076 3295 4757 6487 2028 Size: 1048 219 1462 1730 2409 Fragments arranged by size: 2409 1730 1462 1048 219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2693 4223 844 Size: 1849 1530 3489 Fragments arranged by size: 3489 1849 1530 BsrBI CCG'CTC Cuts at: 1394 1823 4755 1394 Size: 429 2932 3507 Fragments arranged by size: 3507 2932 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2101 5363 5537 65 Size: 1183 853 3262 174 1396 Fragments arranged by size: 3262 1396 1183 853 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5512 5699 1700 Size: 110 3702 187 2869 Fragments arranged by size: 3702 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2755 3109 96 Size: 2659 354 3855 Fragments arranged by size: 3855 2659 354 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6868 BssSI C'ACGA_G Cuts at: 4928 5940 4928 Size: 1012 5856 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6868 BstBI TT'CG_AA Cuts at: 5672 5672 Size: 6868 BstEII G'GTnAC_C Cuts at: 1184 4065 1184 Size: 2881 3987 BstYI r'GATC_y Cuts at: 1972 2458 2539 2597 3238 3427 4198 4565 4950 Size: 486 81 58 641 189 771 367 385 Cuts at: 4950 4967 6419 6505 6517 6863 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 771 641 486 385 367 346 189 86 81 58 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2907 513 Size: 552 939 903 4474 Fragments arranged by size: 4474 939 903 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6129 Fragments arranged by size: 6129 683 56 BtsI GCAGTG_nn' Cuts at: 4384 5178 5198 4384 Size: 794 20 6054 Fragments arranged by size: 6054 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6868 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6833 DraI TTT'AAA Cuts at: 4172 4172 Size: 6868 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5875 809 Size: 507 4559 1802 Fragments arranged by size: 4559 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4315 5204 6833 Size: 54 1425 50 275 18 2485 889 1629 Cuts at: 6833 8 Size: 43 Fragments arranged by size: 2485 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4315 1537 Size: 275 2503 4090 Fragments arranged by size: 4090 2503 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2376 4798 1353 Size: 1023 2422 3423 Fragments arranged by size: 3423 2422 1023 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5455 5829 5975 387 Size: 1064 138 3866 374 146 1280 Fragments arranged by size: 3866 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3155 3155 Size: 6868 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 2525 2592 3254 3338 4931 5528 Size: 393 749 144 67 662 84 1593 597 Cuts at: 5528 6315 6583 1239 Size: 787 268 1524 Fragments arranged by size: 1593 1524 787 749 662 597 393 268 144 84 67 EcoO109I rG'GnC_Cy Cuts at: 2368 2369 2368 Size: 1 6867 EcoRI G'AATT_C Cuts at: 4177 4555 4177 Size: 378 6490 EcoRV GAT'ATC Cuts at: 3702 3702 Size: 6868 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3012 3158 3012 Size: 146 6722 FspI TGC'GCA Cuts at: 5374 5374 Size: 6868 HaeII r_GCGC'y Cuts at: 1678 3524 6015 1678 Size: 1846 2491 2531 Fragments arranged by size: 2531 2491 1846 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2130 2162 2242 2274 2276 Size: 32 135 32 558 32 80 32 2 Cuts at: 2276 2308 2524 2556 3518 3550 5509 5541 5583 Size: 32 216 32 962 32 1959 32 42 Cuts at: 5583 5615 1373 Size: 32 2626 Fragments arranged by size: 2626 1959 962 558 216 135 80 42 32 32 32 32 32 32 32 32 32 2 HincII GTy'rAC Cuts at: 669 1657 1938 2919 3397 4212 4458 669 Size: 988 281 981 478 815 246 3079 Fragments arranged by size: 3079 988 981 815 478 281 246 HindIII A'AGCT_T Cuts at: 748 3416 3956 748 Size: 2668 540 3660 Fragments arranged by size: 3660 2668 540 HpaI GTT'AAC Cuts at: 2919 3397 4458 2919 Size: 478 1061 5329 Fragments arranged by size: 5329 1061 478 KpnI G_GTAC'C Cuts at: 4193 4193 Size: 6868 MfeI C'AATT_G Cuts at: 3436 4467 3436 Size: 1031 5837 MluI A'CGCG_T Cuts at: 6744 6744 Size: 6868 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5982 6166 1305 Size: 4677 184 2007 Fragments arranged by size: 4677 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5046 Fragments arranged by size: 5046 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 3405 4826 5185 5344 518 Size: 648 1479 760 1421 359 159 2042 Fragments arranged by size: 2042 1479 1421 760 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2410 2731 2845 4284 4961 6109 6354 1606 Size: 804 321 114 1439 677 1148 245 2120 Fragments arranged by size: 2120 1439 1148 804 677 321 245 114 NcoI C'CATG_G Cuts at: 513 1065 2004 2907 513 Size: 552 939 903 4474 Fragments arranged by size: 4474 939 903 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6868 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6868 NotI GC'GGCC_GC Cuts at: 4315 4315 Size: 6868 NspI r_CATG'y Cuts at: 1132 1229 2189 2390 2650 4226 5771 1132 Size: 97 960 201 260 1576 1545 2229 Fragments arranged by size: 2229 1576 1545 960 260 201 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6868 PciI A'CATG_T Cuts at: 2646 5767 2646 Size: 3121 3747 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6868 PfoI T'CCnGG_A Cuts at: 2851 2880 2851 Size: 29 6839 PmeI GTTT'AAAC Cuts at: 4172 4172 Size: 6868 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4937 4969 6450 6482 4937 Size: 32 1481 32 5323 Fragments arranged by size: 5323 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2520 1613 Size: 907 5961 PsiI TTA'TAA Cuts at: 3060 4438 3060 Size: 1378 5490 PspOMI G'GGCC_C Cuts at: 2368 2368 Size: 6868 PspXI vC'TCGA_Gb Cuts at: 4025 4025 Size: 6868 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2300 2332 2300 Size: 32 6836 PstI C_TGCA'G Cuts at: 830 2659 4220 830 Size: 1829 1561 3478 Fragments arranged by size: 3478 1829 1561 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6868 PvuII CAG'CTG Cuts at: 2410 2731 2845 2410 Size: 321 114 6433 Fragments arranged by size: 6433 321 114 SacI G_AGCT'C Cuts at: 3157 3157 Size: 6868 SalI G'TCGA_C Cuts at: 1936 4210 1936 Size: 2274 4594 SapI GCTCTTCn'nnn_ Cuts at: 2376 2376 Size: 6868 SbfI CC_TGCA'GG Cuts at: 4220 4220 Size: 6868 ScaI AGT'ACT Cuts at: 5116 6828 5116 Size: 1712 5156 SfcI C'TryA_G Cuts at: 826 1045 2115 2655 4216 5351 6032 6223 826 Size: 219 1070 540 1561 1135 681 191 1471 Fragments arranged by size: 1561 1471 1135 1070 681 540 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6868 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6868 SmaI CCC'GGG Cuts at: 1872 4195 1872 Size: 2323 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2423 4025 4073 4972 5873 6135 Size: 197 926 480 1602 48 899 901 262 Cuts at: 6135 820 Size: 1553 Fragments arranged by size: 1602 1553 926 901 899 480 262 197 48 SnaBI TAC'GTA Cuts at: 493 4659 493 Size: 4166 2702 SpeI A'CTAG_T Cuts at: 152 3881 152 Size: 3729 3139 SphI G_CATG'C Cuts at: 1132 2390 4226 1132 Size: 1258 1836 3774 Fragments arranged by size: 3774 1836 1258 SspI AAT'ATT Cuts at: 5 52 3206 3308 4792 5 Size: 47 3154 102 1484 2081 Fragments arranged by size: 3154 2081 1484 102 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6868 StyI C'CwwG_G Cuts at: 513 1065 2004 2907 4307 513 Size: 552 939 903 1400 3074 Fragments arranged by size: 3074 1400 939 903 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4895 4912 5065 5250 5695 325 Size: 1255 3315 17 153 185 445 1498 Fragments arranged by size: 3315 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2331 2446 2755 Size: 274 80 33 51 1443 354 115 309 Cuts at: 2755 3109 3184 5114 6826 96 Size: 354 75 1930 1712 138 Fragments arranged by size: 1930 1712 1443 354 354 309 274 138 115 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2862 2894 3373 3405 6828 6860 2862 Size: 32 479 32 3423 32 2870 Fragments arranged by size: 3423 2870 479 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6868 XbaI T'CTAG_A Cuts at: 4204 4204 Size: 6868 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3797 3797 Size: 6868 XhoI C'TCGA_G Cuts at: 1943 4025 1943 Size: 2082 4786 XmaI C'CCGG_G Cuts at: 1870 4193 1870 Size: 2323 4545 XmnI GAAnn'nnTTC Cuts at: 2745 3984 4997 2745 Size: 1239 1013 4616 Fragments arranged by size: 4616 1239 1013 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4676 276 Size: 53 83 186 936 3142 2468 Fragments arranged by size: 3142 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI