Restriction map for
pFN21AB2507
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FauI FseI
FspI HapII HgaI Hin1I HincII
HindII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MslI MspA1I MspI MunI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 1066 CGCG CG^CG
AciI 1065 CCGC CCGC(-3/-1)
AspHI 247 GTGCTC G[AT]GC[AT]^C
BalI 716 TGGCCA TGG^CCA
BbsI 37 GTCTTC GAAGAC(2/6)
BclI 818 TGATCA T^GATCA
BmyI 247 GTGCTC G[AGT]GC[ACT]^C
BpmI 1122 CTGGAG CTGGAG(16/14)
BpuAI 37 GTCTTC GAAGAC(2/6)
BsaAI 517 TACGTA [CT]AC^GT[GA]
BsiHKAI 247 GTGCTC G[AT]GC[AT]^C
BsmAI 157 GTCTC GTCTC(1/5)
BsmFI 1106 GGGAC GGGAC(10/14)
BsmI 883 GAATGC GAATGC(1/-1)
Bsp1286I 247 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 258 TGTACA T^GTACA
BspHI 267 TCATGA T^CATGA
BspMI 555 GCAGGT ACCTGC(4/8)
BsrGI 258 TGTACA T^GTACA
BstUI 1066 CGCG CG^CG
BstYI 186 AGATCC [GA]^GATC[CT]
Cac8I 143 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 4 GCGC GCG^C
DraII 1106 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 624 GACAGGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1106 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 20 CCTTGG C^C[AT][AT]GG
EcoT22I 73 ATGCAT ATGCA^T
FbaI 818 TGATCA T^GATCA
HaeII 3 GGCGCT [GA]GCGC^[CT]
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
HindIII 298 AAGCTT A^AGCTT
HphI 471 GGTGA GGTGA(8/7)
MaeII 518 ACGT A^CGT
MflI 186 AGATCC [GA]^GATC[CT]
MluNI 716 TGGCCA TGG^CCA
MscI 716 TGGCCA TGG^CCA
MvnI 1066 CGCG CG^CG
NsiI 73 ATGCAT ATGCA^T
NspI 845 ACATGC [GA]CATG^[CT]
PpuMI 1106 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 267 TCATGA T^CATGA
SfcI 1137 CTACAG C^T[GA][CT]AG
SmlI 761 CTCAAG C^T[CT][GA]AG
SnaBI 517 TACGTA TAC^GTA
SspBI 258 TGTACA T^GTACA
StyI 20 CCTTGG C^C[AT][AT]GG
Tsp45I 622 GTGAC ^GT[GC]AC
XcmI 1111 CCAACTATAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 186 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AseI 361 ATTAAT AT^TAAT
373 ATTAAT
AsnI 361 ATTAAT AT^TAAT
373 ATTAAT
BfaI 329 CTAG C^TAG
1174 CTAG
DraIII 922 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1020 CACACTGTG
EaeI 32 TGGCCG [CT]^GGCC[GA]
716 TGGCCA
EarI 52 CTCTTC CTCTTC(1/4)
161 CTCTTC
HinfI 474 GAATC G^A[ACGT]TC
1069 GAATC
Ksp632I 52 CTCTTC CTCTTC(1/4)
161 CTCTTC
MaeI 329 CTAG C^TAG
1174 CTAG
MaeIII 604 GTAAC ^GT[ACGT]AC
622 GTGAC
NlaIV 1106 GGGACC GG[ACGT]^[ACGT]CC
1107 GGACCC
PshBI 361 ATTAAT AT^TAAT
373 ATTAAT
SfaNI 72 GATGC GCATC(5/9)
325 GATGC
TfiI 474 GAATC G^A[AT]TC
1069 GAATC
TspRI 173 CACTG CA[GC]TG[ACGT][ACGT]^
1022 CACTG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbvI
BsaJI BsiYI BslI BsrDI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
ItaI MboI MboII MnlI MseI
MvaI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI Tru9I TseI Tsp509I