Restriction map for
pFN21AB2507
(Circular) (Six-base) MAPSORT of: pFN21AB2507.seq Check: 5971 from: 1 to: 5887
pFN21AB2507 5887 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3230 1937
Size: 1293 4594
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3950 5334 5602 1632
Size: 2318 1384 268 1917
Fragments arranged by size:
2318 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 4786 5763 1203
Size: 3583 977 1327
Fragments arranged by size:
3583 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2566 2598 2566
Size: 32 5855
AlwNI CAG_nnn'CTG
Cuts at: 3630 5202 3630
Size: 1572 4315
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 3196 3422 3574 5683 1493
Size: 1703 226 152 2109 1697
Fragments arranged by size:
2109 1703 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2367 2379 4441 160
Size: 872 1335 12 2062 1606
Fragments arranged by size:
2062 1606 1335 872 12
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 3212 1870
Size: 73 1269 4545
Fragments arranged by size:
4545 1269 73
BamHI G'GATC_C
Cuts at: 3217 3584 3217
Size: 367 5520
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3208 4663 618
Size: 325 217 627 1421 1455 1842
Fragments arranged by size:
1842 1455 1421 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3206 1391
Size: 1815 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2035 928
Size: 971 136 4780
Fragments arranged by size:
4780 971 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 1903
Size: 34 2141 34 3678
Fragments arranged by size:
3678 2141 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 2823 1548
Size: 1275 4612
BfrBI ATG'CAT
Cuts at: 2080 2080
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5882 5882
Size: 5887
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 3948 5104 1790
Size: 2158 1156 2573
Fragments arranged by size:
2573 2158 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5887
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3618 4576 442
Size: 416 53 83 399 24 2201 958 1753
Fragments arranged by size:
2201 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3148 4547 1239
Size: 1909 1399 2579
Fragments arranged by size:
2579 1909 1399
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2751 4012 4877 5175 2751
Size: 1261 865 298 3463
Fragments arranged by size:
3463 1261 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4550 882
Size: 617 3051 2219
Fragments arranged by size:
3051 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2524 3678 493
Size: 711 1320 1154 2702
Fragments arranged by size:
2702 1320 1154 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3694 4076 4711 275
Size: 53 83 186 936 2161 382 635 1451
Fragments arranged by size:
2161 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4320 4718 4992 5139 5550 4320
Size: 398 274 147 411 4657
Fragments arranged by size:
4657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3121 3151 1178
Size: 30 165 30 1718 30 3914
Fragments arranged by size:
3914 1718 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5887
BseYI C'CCAG_C
Cuts at: 5090 5586 5090
Size: 496 5391
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5314
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 3948 4033 5104 2256
Size: 1692 85 1071 3039
Fragments arranged by size:
3039 1692 1071 85
BsmI GAATG_Cn'
Cuts at: 2894 3398 3491 2894
Size: 504 93 5290
Fragments arranged by size:
5290 504 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 3206 3948 4033 5104 1391
Size: 399 466 950 742 85 1071 2174
Fragments arranged by size:
2174 1071 950 742 466 399 85
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 2272 3776 5506 2272
Size: 1504 1730 2653
Fragments arranged by size:
2653 1730 1504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2551 3242 844
Size: 1707 691 3489
Fragments arranged by size:
3489 1707 691
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2700 2763 2781 2968 4382 4556 65
Size: 1183 1452 63 18 187 1414 174 1396
Fragments arranged by size:
1452 1414 1396 1183 187 174 63 18
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 2263 96
Size: 2167 3720
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5887
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstYI r'GATC_y
Cuts at: 1972 2191 3217 3584 3969 3986 5438 5524 5536
Size: 219 1026 367 385 17 1452 86 12
Cuts at: 5536 5882 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 219 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 3403 4197 4217 3403
Size: 794 20 5073
Fragments arranged by size:
5073 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2752 2787 529
Size: 35 2188 35 3629
Fragments arranged by size:
3629 2188 35 35
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DraIII CAC_nnn'GTG
Cuts at: 2932 3030 2932
Size: 98 5789
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2635 4894 809
Size: 507 1319 2259 1802
Fragments arranged by size:
2259 1802 1319 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2721 3334
Size: 54 1425 50 275 18 207 684 613
Cuts at: 3334 4223 5852 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 684 613 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2172 3817 1353
Size: 710 109 1645 3423
Fragments arranged by size:
3423 1645 710 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4474 4848 4994 387
Size: 1064 138 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3148 3950 4547 5334 5602 1239
Size: 393 1516 802 597 787 268 1524
Fragments arranged by size:
1524 1516 802 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3112 3112
Size: 5887
EcoRI G'AATT_C
Cuts at: 3196 3574 3196
Size: 378 5509
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2273 2273
Size: 5887
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 2012 5034 1678
Size: 334 3022 2531
Fragments arranged by size:
3022 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2097 2129 2580 2612 3121
Size: 32 135 32 525 32 451 32 509
Cuts at: 3121 3153 4528 4560 4602 4634 1373
Size: 32 1375 32 42 32 2626
Fragments arranged by size:
2626 1375 525 509 451 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2303 748
Size: 1555 4332
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2643 2726 5001 5185 1305
Size: 1338 83 2275 184 2007
Fragments arranged by size:
2275 2007 1338 184 83
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2723 10
Size: 54 1425 343 891 3174
Fragments arranged by size:
3174 1425 891 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3845 4204 4363 518
Size: 648 2679 359 159 2042
Fragments arranged by size:
2679 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3303 3980 5128 5373 1606
Size: 1697 677 1148 245 2120
Fragments arranged by size:
2120 1697 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NsiI A_TGCA'T
Cuts at: 2082 2082
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 2854 3245 4790 1132
Size: 97 1625 391 1545 2229
Fragments arranged by size:
2229 1625 1545 391 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5887
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3112 3112
Size: 5887
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2490 2522 2490
Size: 32 5855
PstI C_TGCA'G
Cuts at: 830 3239 830
Size: 2409 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SanDI GG'GwC_CC
Cuts at: 3112 3112
Size: 5887
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SfcI C'TryA_G
Cuts at: 826 1045 3142 3235 4370 5051 5242 826
Size: 219 2097 93 1135 681 191 1471
Fragments arranged by size:
2097 1471 1135 681 219 191 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2766 3991 4892 5154 820
Size: 197 926 823 1225 901 262 1553
Fragments arranged by size:
1553 1225 926 901 823 262 197
SnaBI TAC'GTA
Cuts at: 493 2524 3678 493
Size: 2031 1154 2702
Fragments arranged by size:
2702 2031 1154
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5887
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 3326 513
Size: 552 939 21 1301 3074
Fragments arranged by size:
3074 1301 939 552 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2754 3914 3931 4084 4269 4714 325
Size: 1255 1174 1160 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1174 1160 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2263 2697 4133
Size: 274 80 33 51 1443 286 434 1436
Cuts at: 4133 5845 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1436 434 286 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5887
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3123 2011
Size: 1112 4775
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 4016 4016
Size: 5887
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PvuII RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI