Restriction map for
pFN21AB2507
(Linear) (Six-base) MAPSORT of: KIBB2507.seq Check: 841 from: 1 to: 1182
KIBB2507.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 561 593 1182
Size: 561 32 589
Fragments arranged by size:
589 561 32
AseI AT'TA_AT
Cuts at: 0 362 374 1182
Size: 362 12 808
Fragments arranged by size:
808 362 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 30 1182
Size: 30 1152
BclI T'GATC_A
Cuts at: 0 818 1182
Size: 818 364
BfrBI ATG'CAT
Cuts at: 0 75 1182
Size: 75 1107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1143 1182
Size: 1143 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1182
Size: 746 436
BsaAI yAC'GTr
Cuts at: 0 519 1182
Size: 519 663
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1116 1146 1182
Size: 1116 30 36
Fragments arranged by size:
1116 36 30
BsiHKAI G_wGCw'C
Cuts at: 0 251 1182
Size: 251 931
BsmI GAATG_Cn'
Cuts at: 0 889 1182
Size: 889 293
Bsp1286I G_dGCh'C
Cuts at: 0 251 1182
Size: 251 931
BspHI T'CATG_A
Cuts at: 0 267 1182
Size: 267 915
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 546 1182
Size: 546 636
BsrDI GCAATG_nn'
Cuts at: 0 695 758 776 963 1182
Size: 695 63 18 187 219
Fragments arranged by size:
695 219 187 63 18
BsrGI T'GTAC_A
Cuts at: 0 258 1182
Size: 258 924
BstYI r'GATC_y
Cuts at: 0 186 1182
Size: 186 996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 747 782 1182
Size: 747 35 400
Fragments arranged by size:
747 400 35
DraIII CAC_nnn'GTG
Cuts at: 0 927 1025 1182
Size: 927 98 157
Fragments arranged by size:
927 157 98
DrdI GACnn_nn'nnGTC
Cuts at: 0 630 1182
Size: 630 552
EaeI y'GGCC_r
Cuts at: 0 32 716 1182
Size: 32 684 466
Fragments arranged by size:
684 466 32
EarI CTCTTCn'nnn_
Cuts at: 0 58 167 1182
Size: 58 109 1015
Fragments arranged by size:
1015 109 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1143 1182
Size: 1143 39
EcoO109I rG'GnC_Cy
Cuts at: 0 1107 1182
Size: 1107 75
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 268 1182
Size: 268 914
HaeII r_GCGC'y
Cuts at: 0 7 1182
Size: 7 1175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 575 607 1116 1148 1182
Size: 92 32 451 32 509 32 34
Fragments arranged by size:
509 451 92 34 32 32 32
HindIII A'AGCT_T
Cuts at: 0 298 1182
Size: 298 884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 721 1182
Size: 638 83 461
Fragments arranged by size:
638 461 83
MscI TGG'CCA
Cuts at: 0 718 1182
Size: 718 464
NsiI A_TGCA'T
Cuts at: 0 77 1182
Size: 77 1105
NspI r_CATG'y
Cuts at: 0 849 1182
Size: 849 333
PpuMI rG'GwC_Cy
Cuts at: 0 1107 1182
Size: 1107 75
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 485 517 1182
Size: 485 32 665
Fragments arranged by size:
665 485 32
SanDI GG'GwC_CC
Cuts at: 0 1107 1182
Size: 1107 75
SfcI C'TryA_G
Cuts at: 0 1137 1182
Size: 1137 45
SmlI C'TyrA_G
Cuts at: 0 761 1182
Size: 761 421
SnaBI TAC'GTA
Cuts at: 0 519 1182
Size: 519 663
StyI C'CwwG_G
Cuts at: 0 20 1182
Size: 20 1162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 749 1182
Size: 749 433
TatI w'GTAC_w
Cuts at: 0 258 692 1182
Size: 258 434 490
Fragments arranged by size:
490 434 258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1118 1182
Size: 1118 64
Enzymes that do cut:
AloI AseI BbsI BclI BfrBI BpmI BpuEI BsaAI
BsaXI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BstYI CspCI DraIII DrdI EaeI EarI Eco57MI EcoO109I
FalI HaeII Hin4I HindIII MmeI MscI NsiI NspI
PpuMI PsrI SanDI SfcI SmlI SnaBI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI