Restriction map for
pFN21AB2501
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspMI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI TfiI
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AlwNI 1403 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 549 GGACC G^G[AT]CC
BalI 962 TGGCCA TGG^CCA
BbrPI 1749 CACGTG CAC^GTG
BsaAI 1749 CACGTG [CT]AC^GT[GA]
BsgI 367 CTGCAC GTGCAG(16/14)
BsrBI 394 CCGCTC CCGCTC(-3/-3)
BstAPI 1822 GCAGGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 655 TTCGAA TT^CGAA
DraIII 1559 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 294 CGGCCG C^GGCCG
EclXI 294 CGGCCG C^GGCCG
Eco52I 294 CGGCCG C^GGCCG
EcoRI 925 GAATTC G^AATTC
HinfI 992 GACTC G^A[ACGT]TC
KspI 261 CCGCGG CCGC^GG
MluNI 962 TGGCCA TGG^CCA
MscI 962 TGGCCA TGG^CCA
NotI 293 GCGGCCGC GC^GGCCGC
NspV 655 TTCGAA TT^CGAA
PleI 992 GACTC GAGTC(4/5)
PmaCI 1749 CACGTG CAC^GTG
PmlI 1749 CACGTG CAC^GTG
PpuMI 548 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1567 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1220 GAGCTC GAGCT^C
SacII 261 CCGCGG CCGC^GG
SapI 396 GCTCTTC GCTCTTC(1/4)
ScaI 1132 AGTACT AGT^ACT
SfuI 655 TTCGAA TT^CGAA
SmlI 76 CTCAAG C^T[CT][GA]AG
SphI 1349 GCATGC GCATG^C
SspI 763 AATATT AAT^ATT
XcmI 1301 CCATGACTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 325 GGCGTC G[GA]^CG[CT]C
481 GACGCC
AocI 1038 CCTCAGG CC^T[ACGT]AGG
1501 CCTCAGG
ApaI 597 GGGCCC GGGCC^C
647 GGGCCC
BamHI 834 GGATCC G^GATCC
1061 GGATCC
BanI 46 GGCACC G^G[CT][GA]CC
317 GGCACC
BbsI 658 GAAGAC GAAGAC(2/6)
328 GTCTTC
BglI 484 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
599 GCCCCGACGGC
BpuAI 658 GAAGAC GAAGAC(2/6)
328 GTCTTC
BsaHI 325 GGCGTC G[GA]^CG[CT]C
481 GACGCC
BsaI 433 GAGACC GGTCTC(1/5)
508 GAGACC
BsiEI 294 CGGCCG CG[GA][CT]^CG
312 CGACCG
BsmFI 990 GGGAC GGGAC(10/14)
210 GTCCC
BspLU11I 1570 ACATGT A^CATGT
1720 ACATGT
BsrDI 1447 GCAATG GCAATG(2/0)
1826 GCAATG
BstXI 282 CCATCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
560 CCAAGAGGCTGG
BstYI 834 GGATCC [GA]^GATC[CT]
1061 GGATCC
Bsu36I 1038 CCTCAGG CC^T[ACGT]AGG
1501 CCTCAGG
DsaI 143 CCACGG C^C[GA][CT]GG
261 CCGCGG
Eco81I 1038 CCTCAGG CC^T[ACGT]AGG
1501 CCTCAGG
EcoT14I 1442 CCTTGG C^C[AT][AT]GG
1468 CCAAGG
Hin1I 325 GGCGTC G[GA]^CG[CT]C
481 GACGCC
HindIII 755 AAGCTT A^AGCTT
1797 AAGCTT
MflI 834 GGATCC [GA]^GATC[CT]
1061 GGATCC
SfaNI 425 GCATC GCATC(5/9)
572 GCATC
SmaI 214 CCCGGG CCC^GGG
321 CCCGGG
StyI 1442 CCTTGG C^C[AT][AT]GG
1468 CCAAGG
XhoII 834 GGATCC [GA]^GATC[CT]
1061 GGATCC
XmaCI 214 CCCGGG C^CCGGG
321 CCCGGG
XmaI 214 CCCGGG C^CCGGG
321 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PstI RsaI Sau3AI
Sau96I ScrFI SfcI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I