(Circular) (Six-base) MAPSORT of: pFN21AB2501.seq Check: 6932 from: 1 to: 6619 pFN21AB2501 6619 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4429 278 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3962 1937 Size: 2025 4594 Acc65I G'GTAC_C Cuts at: 3940 3940 Size: 6619 AclI AA'CG_TT Cuts at: 1563 4746 5119 1563 Size: 3183 373 3063 Fragments arranged by size: 3183 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3228 4682 6066 6334 1632 Size: 1596 1454 1384 268 1917 Fragments arranged by size: 1917 1596 1454 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 2388 3575 3725 3753 3755 5518 6495 1203 Size: 1185 1187 150 28 2 1763 977 1327 Fragments arranged by size: 1763 1327 1187 1185 977 150 28 2 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6619 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2407 1184 Size: 1223 5396 AlwNI CAG_nnn'CTG Cuts at: 3413 4362 5934 3413 Size: 949 1572 4098 Fragments arranged by size: 4098 1572 949 ApaI G_GGCC'C Cuts at: 2606 2656 2606 Size: 50 6569 ApaLI G'TGCA_C Cuts at: 4676 5832 4676 Size: 1156 5463 ApoI r'AATT_y Cuts at: 1493 2891 2930 3075 3146 3928 4154 4306 6415 Size: 1398 39 145 71 782 226 152 2109 Cuts at: 6415 1493 Size: 1697 Fragments arranged by size: 2109 1697 1398 782 226 152 145 71 39 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 2219 2326 2597 3944 1870 Size: 73 276 107 271 1347 4545 Fragments arranged by size: 4545 1347 276 271 107 73 BamHI G'GATC_C Cuts at: 2839 3066 3949 4316 2839 Size: 227 883 367 5142 Fragments arranged by size: 5142 883 367 227 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2322 3940 5395 618 Size: 325 217 627 264 271 1618 1455 1842 Fragments arranged by size: 1842 1618 1455 627 325 271 264 217 BanII G_rGCy'C Cuts at: 1391 2130 2454 2598 2606 2656 3229 3370 3938 Size: 739 324 144 8 50 573 141 568 Cuts at: 3938 1391 Size: 4072 Fragments arranged by size: 4072 739 573 568 324 144 141 50 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2326 2670 928 Size: 971 427 344 4877 Fragments arranged by size: 4877 971 427 344 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4810 4844 1903 Size: 34 2873 34 3678 Fragments arranged by size: 3678 2873 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4511 5721 4511 Size: 1210 5409 BclI T'GATC_A Cuts at: 1548 1548 Size: 6619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2495 2610 5230 Size: 107 122 71 631 605 823 115 2620 Cuts at: 5230 136 Size: 1525 Fragments arranged by size: 2620 1525 823 631 605 122 115 107 71 BglII A'GATC_T Cuts at: 6614 6614 Size: 6619 BlpI GC'TnA_GC Cuts at: 4036 4036 Size: 6619 Bme1580I G_kGCm'C Cuts at: 1790 2054 2606 2656 4680 5836 1790 Size: 264 552 50 2024 1156 2573 Fragments arranged by size: 2573 2024 1156 552 264 50 BmgBI CAC'GTC Cuts at: 1318 2389 2446 1318 Size: 1071 57 5491 Fragments arranged by size: 5491 1071 57 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4350 5308 442 Size: 416 53 83 399 24 2933 958 1753 Fragments arranged by size: 2933 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3864 3909 3864 Size: 45 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2647 3405 3834 5279 1239 Size: 1408 758 429 1445 2579 Fragments arranged by size: 2579 1445 1408 758 429 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2066 4744 5609 5907 2066 Size: 2678 865 298 2778 Fragments arranged by size: 2778 2678 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2432 2507 5282 882 Size: 617 933 75 2775 2219 Fragments arranged by size: 2775 2219 933 617 75 BsaAI yAC'GTr Cuts at: 493 1204 3756 4410 493 Size: 711 2552 654 2702 Fragments arranged by size: 2702 2552 711 654 BsaBI GATnn'nnATC Cuts at: 6613 6613 Size: 6619 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2331 2487 4426 4808 Size: 53 83 186 936 798 156 1939 382 Cuts at: 4808 5443 275 Size: 635 1451 Fragments arranged by size: 1939 1451 936 798 635 382 186 156 83 53 BsaWI w'CCGG_w Cuts at: 5052 5450 5724 5871 6282 5052 Size: 398 274 147 411 5389 Fragments arranged by size: 5389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2856 2886 1178 Size: 30 165 30 1453 30 4911 Fragments arranged by size: 4911 1453 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2110 2423 2426 2507 2644 3495 1186 Size: 924 313 3 81 137 851 4310 Fragments arranged by size: 4310 924 851 313 137 81 3 BseYI C'CCAG_C Cuts at: 2572 5822 6318 2572 Size: 3250 496 2873 Fragments arranged by size: 3250 2873 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 1163 Size: 573 621 5425 Fragments arranged by size: 5425 621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2302 2320 4069 4830 5858 1540 Size: 275 186 301 18 1749 761 1028 2301 Fragments arranged by size: 2301 1749 1028 761 301 275 186 18 BsiHKAI G_wGCw'C Cuts at: 2349 3229 3258 3379 4680 4765 5836 2349 Size: 880 29 121 1301 85 1071 3132 Fragments arranged by size: 3132 1301 1071 880 121 85 29 BsmI GAATG_Cn' Cuts at: 4130 4223 4130 Size: 93 6526 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2130 2349 2454 2598 2606 2656 Size: 399 264 76 219 105 144 8 50 Cuts at: 2656 3229 3258 3370 3379 3938 4680 4765 5836 Size: 573 29 112 9 559 742 85 1071 Cuts at: 5836 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 573 559 399 264 219 144 112 105 85 76 50 29 9 8 BspEI T'CCGG_A Cuts at: 6282 6282 Size: 6619 BspHI T'CATG_A Cuts at: 4508 6238 4508 Size: 1730 4889 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3974 844 Size: 3130 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2401 4506 1394 Size: 429 578 2105 3507 Fragments arranged by size: 3507 2105 578 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3459 3838 5114 5288 65 Size: 1183 2211 379 1276 174 1396 Fragments arranged by size: 2211 1396 1276 1183 379 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2319 2363 2472 5263 5450 1700 Size: 110 509 44 109 2791 187 2869 Fragments arranged by size: 2869 2791 509 187 110 109 44 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6619 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 4679 5691 4679 Size: 1012 5607 BstAPI GCAn_nnn'nTGC Cuts at: 794 3833 794 Size: 3039 3580 BstBI TT'CG_AA Cuts at: 2661 5423 2661 Size: 2762 3857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6619 BstXI CCAn_nnnn'nTGG Cuts at: 2294 2572 2294 Size: 278 6341 BstYI r'GATC_y Cuts at: 1972 2839 3066 3949 4316 4701 4718 6170 6256 Size: 867 227 883 367 385 17 1452 86 Cuts at: 6256 6268 6614 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 883 867 385 367 346 227 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3044 3507 3044 Size: 463 6156 BtgI C'CryG_G Cuts at: 513 1065 2004 2148 2266 513 Size: 552 939 144 118 4866 Fragments arranged by size: 4866 939 552 144 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5880 Fragments arranged by size: 5880 683 56 BtsI GCAGTG_nn' Cuts at: 2374 4135 4929 4949 2374 Size: 1761 794 20 4044 Fragments arranged by size: 4044 1761 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6584 DraI TTT'AAA Cuts at: 3923 3923 Size: 6619 DraIII CAC_nnn'GTG Cuts at: 3569 3569 Size: 6619 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5626 809 Size: 507 4310 1802 Fragments arranged by size: 4310 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2299 2562 2620 Size: 54 1425 50 275 18 469 263 58 Cuts at: 2620 2967 4066 4955 6584 8 Size: 347 1099 889 1629 43 Fragments arranged by size: 1629 1425 1099 889 469 347 275 263 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2299 4066 1537 Size: 275 487 1767 4090 Fragments arranged by size: 4090 1767 487 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2408 2663 3132 3709 4549 1353 Size: 1055 255 469 577 840 3423 Fragments arranged by size: 3423 1055 840 577 469 255 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5206 5580 5726 387 Size: 1064 138 3617 374 146 1280 Fragments arranged by size: 3617 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3227 3227 Size: 6619 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2647 3228 3405 3834 4682 5279 6066 Size: 393 1015 581 177 429 848 597 787 Cuts at: 6066 6334 1239 Size: 268 1524 Fragments arranged by size: 1524 1015 848 787 597 581 429 393 268 177 EcoO109I rG'GnC_Cy Cuts at: 2554 2602 2603 2653 2554 Size: 48 1 50 6520 Fragments arranged by size: 6520 50 48 1 EcoRI G'AATT_C Cuts at: 2930 3928 4306 2930 Size: 998 378 5243 Fragments arranged by size: 5243 998 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3050 3050 Size: 6619 FspI TGC'GCA Cuts at: 5125 5125 Size: 6619 HaeII r_GCGC'y Cuts at: 1678 2207 2501 3892 5766 1678 Size: 529 294 1391 1874 2531 Fragments arranged by size: 2531 1874 1391 529 294 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2600 2632 2657 2689 3888 Size: 32 135 32 1028 32 25 32 1199 Cuts at: 3888 3920 5260 5292 5334 5366 1373 Size: 32 1340 32 42 32 2626 Fragments arranged by size: 2626 1340 1199 1028 135 42 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3963 4209 669 Size: 988 281 2025 246 3079 Fragments arranged by size: 3079 2025 988 281 246 HindIII A'AGCT_T Cuts at: 748 2760 3802 748 Size: 2012 1042 3565 Fragments arranged by size: 3565 2012 1042 HpaI GTT'AAC Cuts at: 4209 4209 Size: 6619 KpnI G_GTAC'C Cuts at: 3944 3944 Size: 6619 MfeI C'AATT_G Cuts at: 4218 4218 Size: 6619 MluI A'CGCG_T Cuts at: 6495 6495 Size: 6619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5733 5917 1305 Size: 4428 184 2007 Fragments arranged by size: 4428 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2969 10 Size: 54 1425 343 1137 3660 Fragments arranged by size: 3660 1425 1137 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2292 3359 3407 4577 4936 5095 518 Size: 648 1126 1067 48 1170 359 159 2042 Fragments arranged by size: 2042 1170 1126 1067 648 359 159 48 MspA1I CmG'CkG Cuts at: 1606 2212 2268 2625 4035 4712 5860 6105 1606 Size: 606 56 357 1410 677 1148 245 2120 Fragments arranged by size: 2120 1410 1148 677 606 357 245 56 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5128 Fragments arranged by size: 5128 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6619 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 2299 4066 2299 Size: 1767 4852 NspI r_CATG'y Cuts at: 1132 1229 2650 3358 3579 3729 3977 5522 1132 Size: 97 1421 708 221 150 248 1545 2229 Fragments arranged by size: 2229 1545 1421 708 248 221 150 97 PasI CC'CwG_GG Cuts at: 1348 3316 1348 Size: 1968 4651 PciI A'CATG_T Cuts at: 3575 3725 5518 3575 Size: 150 1793 4676 Fragments arranged by size: 4676 1793 150 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6619 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PmlI CAC'GTG Cuts at: 3756 3756 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4688 4720 6201 6233 4688 Size: 32 1481 32 5074 Fragments arranged by size: 5074 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2554 2554 Size: 6619 PshAI GACnn'nnGTC Cuts at: 1613 3576 1613 Size: 1963 4656 PsiI TTA'TAA Cuts at: 4189 4189 Size: 6619 PspOMI G'GGCC_C Cuts at: 2602 2652 2602 Size: 50 6569 PstI C_TGCA'G Cuts at: 830 2169 2261 2640 3971 830 Size: 1339 92 379 1331 3478 Fragments arranged by size: 3478 1339 1331 379 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 SacI G_AGCT'C Cuts at: 3229 3229 Size: 6619 SacII CC_GC'GG Cuts at: 2269 2269 Size: 6619 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SapI GCTCTTCn'nnn_ Cuts at: 2408 2408 Size: 6619 SbfI CC_TGCA'GG Cuts at: 3971 3971 Size: 6619 ScaI AGT'ACT Cuts at: 3139 4867 6579 3139 Size: 1728 1712 3179 Fragments arranged by size: 3179 1728 1712 SfcI C'TryA_G Cuts at: 826 1045 2165 2257 2636 2819 3297 3967 5102 Size: 219 1120 92 379 183 478 670 1135 Cuts at: 5102 5783 5974 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1120 681 670 478 379 219 191 183 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 2221 2328 3946 1872 Size: 349 107 1618 4545 Fragments arranged by size: 4545 1618 349 107 SmlI C'TyrA_G Cuts at: 820 1017 1943 2081 4723 5624 5886 820 Size: 197 926 138 2642 901 262 1553 Fragments arranged by size: 2642 1553 926 901 262 197 138 SnaBI TAC'GTA Cuts at: 493 4410 493 Size: 3917 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3358 3977 1132 Size: 2226 619 3774 Fragments arranged by size: 3774 2226 619 SspI AAT'ATT Cuts at: 5 52 2770 4543 5 Size: 47 2718 1773 2081 Fragments arranged by size: 2718 2081 1773 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6619 StyI C'CwwG_G Cuts at: 513 1065 2004 3447 3473 4058 513 Size: 552 939 1443 26 585 3074 Fragments arranged by size: 3074 1443 939 585 552 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2456 4646 4663 4816 5001 5446 325 Size: 1255 876 2190 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 876 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3137 3438 3560 Size: 274 80 33 51 1443 1160 301 122 Cuts at: 3560 3638 4865 6577 96 Size: 78 1227 1712 138 Fragments arranged by size: 1712 1443 1227 1160 301 274 138 122 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2856 2888 6579 6611 2856 Size: 32 3691 32 2864 Fragments arranged by size: 3691 2864 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6619 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3313 3313 Size: 6619 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 2219 2326 3944 1870 Size: 349 107 1618 4545 Fragments arranged by size: 4545 1618 349 107 XmnI GAAnn'nnTTC Cuts at: 4748 4748 Size: 6619 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4427 276 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI