Restriction map for
pFN21AB2501
(Linear) (Six-base) MAPSORT of: KIBB2501.seq Check: 8245 from: 1 to: 1914
KIBB2501.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1223 1914
Size: 1223 691
AflIII A'CryG_T
Cuts at: 0 383 1570 1720 1748 1750 1914
Size: 383 1187 150 28 2 164
Fragments arranged by size:
1187 383 164 150 28 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 402 1914
Size: 402 1512
AlwNI CAG_nnn'CTG
Cuts at: 0 1408 1914
Size: 1408 506
ApaI G_GGCC'C
Cuts at: 0 601 651 1914
Size: 601 50 1263
Fragments arranged by size:
1263 601 50
ApoI r'AATT_y
Cuts at: 0 886 925 1070 1141 1914
Size: 886 39 145 71 773
Fragments arranged by size:
886 773 145 71 39
AvaI C'yCGr_G
Cuts at: 0 214 321 592 1914
Size: 214 107 271 1322
Fragments arranged by size:
1322 271 214 107
BamHI G'GATC_C
Cuts at: 0 834 1061 1914
Size: 834 227 853
Fragments arranged by size:
853 834 227
BanI G'GyrC_C
Cuts at: 0 46 317 1914
Size: 46 271 1597
Fragments arranged by size:
1597 271 46
BanII G_rGCy'C
Cuts at: 0 125 449 593 601 651 1224 1365 1914
Size: 125 324 144 8 50 573 141 549
Fragments arranged by size:
573 549 324 144 141 125 50 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 321 665 1914
Size: 321 344 1249
Fragments arranged by size:
1249 344 321
BglI GCCn_nnn'nGGC
Cuts at: 0 490 605 1914
Size: 490 115 1309
Fragments arranged by size:
1309 490 115
Bme1580I G_kGCm'C
Cuts at: 0 49 601 651 1914
Size: 49 552 50 1263
Fragments arranged by size:
1263 552 50 49
BmgBI CAC'GTC
Cuts at: 0 384 441 1914
Size: 384 57 1473
Fragments arranged by size:
1473 384 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1859 1904 1914
Size: 1859 45 10
Fragments arranged by size:
1859 45 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 1400 1829 1914
Size: 642 758 429 85
Fragments arranged by size:
758 642 429 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1914
Size: 61 1853
BsaI GGTCTCn'nnnn_
Cuts at: 0 427 502 1914
Size: 427 75 1412
Fragments arranged by size:
1412 427 75
BsaAI yAC'GTr
Cuts at: 0 1751 1914
Size: 1751 163
BsaHI Gr'CG_yC
Cuts at: 0 326 482 1914
Size: 326 156 1432
Fragments arranged by size:
1432 326 156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 851 881 1914
Size: 851 30 1033
Fragments arranged by size:
1033 851 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 105 418 421 502 639 1490 1914
Size: 105 313 3 81 137 851 424
Fragments arranged by size:
851 424 313 137 105 81 3
BseYI C'CCAG_C
Cuts at: 0 567 1914
Size: 567 1347
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1914
Size: 352 1562
BsiEI CG_ry'CG
Cuts at: 0 297 315 1914
Size: 297 18 1599
Fragments arranged by size:
1599 297 18
BsiHKAI G_wGCw'C
Cuts at: 0 344 1224 1253 1374 1914
Size: 344 880 29 121 540
Fragments arranged by size:
880 540 344 121 29
Bsp1286I G_dGCh'C
Cuts at: 0 49 125 344 449 593 601 651 1224
Size: 49 76 219 105 144 8 50 573
Cuts at: 1224 1253 1365 1374 1914
Size: 29 112 9 540
Fragments arranged by size:
573 540 219 144 112 105 76 50
49 29 9 8
BsrBI CCG'CTC
Cuts at: 0 396 1914
Size: 396 1518
BsrDI GCAATG_nn'
Cuts at: 0 1454 1833 1914
Size: 1454 379 81
Fragments arranged by size:
1454 379 81
BsrFI r'CCGG_y
Cuts at: 0 314 358 467 1914
Size: 314 44 109 1447
Fragments arranged by size:
1447 314 109 44
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1828 1914
Size: 1828 86
BstBI TT'CG_AA
Cuts at: 0 656 1914
Size: 656 1258
BstXI CCAn_nnnn'nTGG
Cuts at: 0 289 567 1914
Size: 289 278 1347
Fragments arranged by size:
1347 289 278
BstYI r'GATC_y
Cuts at: 0 834 1061 1914
Size: 834 227 853
Fragments arranged by size:
853 834 227
Bsu36I CC'TnA_GG
Cuts at: 0 1039 1502 1914
Size: 1039 463 412
Fragments arranged by size:
1039 463 412
BtgI C'CryG_G
Cuts at: 0 143 261 1914
Size: 143 118 1653
Fragments arranged by size:
1653 143 118
BtsI GCAGTG_nn'
Cuts at: 0 369 1914
Size: 369 1545
DraIII CAC_nnn'GTG
Cuts at: 0 1564 1914
Size: 1564 350
EaeI y'GGCC_r
Cuts at: 0 294 557 615 962 1914
Size: 294 263 58 347 952
Fragments arranged by size:
952 347 294 263 58
EagI C'GGCC_G
Cuts at: 0 294 1914
Size: 294 1620
EarI CTCTTCn'nnn_
Cuts at: 0 403 658 1127 1704 1914
Size: 403 255 469 577 210
Fragments arranged by size:
577 469 403 255 210
EcoICRI GAG'CTC
Cuts at: 0 1222 1914
Size: 1222 692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 1223 1400 1829 1914
Size: 642 581 177 429 85
Fragments arranged by size:
642 581 429 177 85
EcoO109I rG'GnC_Cy
Cuts at: 0 549 597 598 648 1914
Size: 549 48 1 50 1266
Fragments arranged by size:
1266 549 50 48 1
EcoRI G'AATT_C
Cuts at: 0 925 1914
Size: 925 989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1045 1914
Size: 1045 869
HaeII r_GCGC'y
Cuts at: 0 202 496 1887 1914
Size: 202 294 1391 27
Fragments arranged by size:
1391 294 202 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 652 684 1883 1914
Size: 595 32 25 32 1199 31
Fragments arranged by size:
1199 595 32 32 31 25
HindIII A'AGCT_T
Cuts at: 0 755 1797 1914
Size: 755 1042 117
Fragments arranged by size:
1042 755 117
MscI TGG'CCA
Cuts at: 0 964 1914
Size: 964 950
MslI CAynn'nnrTG
Cuts at: 0 287 1354 1402 1914
Size: 287 1067 48 512
Fragments arranged by size:
1067 512 287 48
MspA1I CmG'CkG
Cuts at: 0 207 263 620 1914
Size: 207 56 357 1294
Fragments arranged by size:
1294 357 207 56
NotI GC'GGCC_GC
Cuts at: 0 294 1914
Size: 294 1620
NspI r_CATG'y
Cuts at: 0 645 1353 1574 1724 1914
Size: 645 708 221 150 190
Fragments arranged by size:
708 645 221 190 150
PasI CC'CwG_GG
Cuts at: 0 1311 1914
Size: 1311 603
PciI A'CATG_T
Cuts at: 0 1570 1720 1914
Size: 1570 150 194
Fragments arranged by size:
1570 194 150
PmlI CAC'GTG
Cuts at: 0 1751 1914
Size: 1751 163
PpuMI rG'GwC_Cy
Cuts at: 0 549 1914
Size: 549 1365
PshAI GACnn'nnGTC
Cuts at: 0 1571 1914
Size: 1571 343
PspOMI G'GGCC_C
Cuts at: 0 597 647 1914
Size: 597 50 1267
Fragments arranged by size:
1267 597 50
PstI C_TGCA'G
Cuts at: 0 164 256 635 1914
Size: 164 92 379 1279
Fragments arranged by size:
1279 379 164 92
SacI G_AGCT'C
Cuts at: 0 1224 1914
Size: 1224 690
SacII CC_GC'GG
Cuts at: 0 264 1914
Size: 264 1650
SapI GCTCTTCn'nnn_
Cuts at: 0 403 1914
Size: 403 1511
ScaI AGT'ACT
Cuts at: 0 1134 1914
Size: 1134 780
SfcI C'TryA_G
Cuts at: 0 160 252 631 814 1292 1914
Size: 160 92 379 183 478 622
Fragments arranged by size:
622 478 379 183 160 92
SmaI CCC'GGG
Cuts at: 0 216 323 1914
Size: 216 107 1591
Fragments arranged by size:
1591 216 107
SmlI C'TyrA_G
Cuts at: 0 76 1914
Size: 76 1838
SphI G_CATG'C
Cuts at: 0 1353 1914
Size: 1353 561
SspI AAT'ATT
Cuts at: 0 765 1914
Size: 765 1149
StyI C'CwwG_G
Cuts at: 0 1442 1468 1914
Size: 1442 26 446
Fragments arranged by size:
1442 446 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 451 1914
Size: 451 1463
TatI w'GTAC_w
Cuts at: 0 1132 1433 1555 1633 1914
Size: 1132 301 122 78 281
Fragments arranged by size:
1132 301 281 122 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 851 883 1914
Size: 851 32 1031
Fragments arranged by size:
1031 851 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1308 1914
Size: 1308 606
XmaI C'CCGG_G
Cuts at: 0 214 321 1914
Size: 214 107 1593
Fragments arranged by size:
1593 214 107
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BglI Bme1580I BmgBI BplI BpmI
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HindIII MscI MslI MspA1I NotI NspI PasI PciI
PmlI PpuMI PshAI PspOMI PstI SacI SacII SapI
ScaI SfcI SmaI SmlI SphI SspI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI Bpu10I BsaBI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI BstEII
BstZ17I BtgZI ClaI CspCI DraI DrdI EciI EcoNI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI PacI PflMI PfoI PmeI PpiI
PsiI PspXI PsrI PvuI PvuII RsrII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI StuI SwaI Tth111I XbaI XhoI XmnI ZraI