Restriction map for
pFN21AB1569
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AclI AcyI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BsaHI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
Bsu36I CfoI Cfr10I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KspI MluI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 645 GGTACC G^GTACC
AccII 2303 CGCG CG^CG
AccIII 2645 TCCGGA T^CCGGA
AhdI 655 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 61 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 645 GGTACC G^GTACC
AspEI 655 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1949 TGGCCA TGG^CCA
BanI 645 GGTACC G^G[CT][GA]CC
BbsI 1807 GAAGAC GAAGAC(2/6)
BlpI 1251 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1251 GCTGAGC GC^T[ACGT]AGC
BpuAI 1807 GAAGAC GAAGAC(2/6)
BsaWI 2645 TCCGGA [AT]^CCGG[AT]
BseAI 2645 TCCGGA T^CCGGA
BsmI 323 GCATTC GAATGC(1/-1)
BspEI 2645 TCCGGA T^CCGGA
Bst1107I 589 GTATAC GTA^TAC
BstUI 2303 CGCG CG^CG
BstZ17I 589 GTATAC GTA^TAC
CelII 1251 GCTGAGC GC^T[ACGT]AGC
DraI 1761 TTTAAA TTT^AAA
DrdI 1405 GACTACGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1949 TGGCCA [CT]^GGCC[GA]
Eam1105I 655 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 157 GAAGAG CTCTTC(1/4)
EcoNI 666 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2725 GATATC GAT^ATC
FauI 870 CCCGC CCCGC(4/6)
KpnI 645 GGTACC GGTAC^C
Ksp632I 157 GAAGAG CTCTTC(1/4)
MfeI 237 CAATTG C^AATTG
MluNI 1949 TGGCCA TGG^CCA
MroI 2645 TCCGGA T^CCGGA
MscI 1949 TGGCCA TGG^CCA
MunI 237 CAATTG C^AATTG
MvnI 2303 CGCG CG^CG
PshAI 223 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 818 GTCGAC G^TCGAC
SpeI 1582 ACTAGT A^CTAGT
XbaI 2913 TCTAGA T^CTAGA
XcmI 142 CCATCACATGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 61 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 589 GTATAC GT^[AC][GT]AC
818 GTCGAC
AcsI 400 AAATTT [GA]^AATT[CT]
1891 GAATTT
ApoI 400 AAATTT [GA]^AATT[CT]
1891 GAATTT
AspHI 292 GAGCTC G[AT]GC[AT]^C
1254 GAGCTC
BclI 387 TGATCA T^GATCA
2258 TGATCA
BcnI 933 CCGGG CC^[GC]GG
2653 CCCGG
BsaAI 1513 TACGTA [CT]AC^GT[GA]
2132 TACGTA
BsaBI 382 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2355 GATCCCAATC
BsgI 1905 GTGCAG GTGCAG(16/14)
2678 GTGCAG
BsiHKAI 292 GAGCTC G[AT]GC[AT]^C
1254 GAGCTC
BspHI 1628 TCATGA T^CATGA
1827 TCATGA
BspMI 911 GCAGGT ACCTGC(4/8)
1885 GCAGGT
BstXI 1838 CCAAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2359 CCAATCAGGTGG
DraII 257 GGGTCCC [GA]G^G[ACGT]CC[CT]
513 AGGTCCT
EcoO109I 257 GGGTCCC [GA]G^G[ACGT]CC[CT]
513 AGGTCCT
FbaI 387 TGATCA T^GATCA
2258 TGATCA
HindIII 812 AAGCTT A^AGCTT
2638 AAGCTT
MamI 382 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2355 GATCCCAATC
NciI 933 CCGGG CC^[GC]GG
2653 CCCGG
NdeI 1083 CATATG CA^TATG
1802 CATATG
PflMI 142 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
660 CCAGTCCCTGG
PpuMI 257 GGGTCCC [GA]G^G[AT]CC[CT]
513 AGGTCCT
PstI 729 CTGCAG CTGCA^G
909 CTGCAG
RcaI 1628 TCATGA T^CATGA
1827 TCATGA
SacI 292 GAGCTC GAGCT^C
1254 GAGCTC
SexAI 203 ACCAGGT A^CC[AT]GGT
822 ACCAGGT
SmlI 295 CTCAAG C^T[CT][GA]AG
1495 CTCAAG
SnaBI 1513 TACGTA TAC^GTA
2132 TACGTA
Van91I 142 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
660 CCAGTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII