(Circular) (Six-base) MAPSORT of: pFN21AB1569.seq Check: 8969 from: 1 to: 7654 pFN21AB1569 7654 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2907 3881 2907 Size: 974 6680 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5464 278 Size: 53 83 186 936 3928 2468 Fragments arranged by size: 3928 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2595 2824 4997 1937 Size: 658 229 2173 4594 Fragments arranged by size: 4594 2173 658 229 Acc65I G'GTAC_C Cuts at: 2650 4975 2650 Size: 2325 5329 AclI AA'CG_TT Cuts at: 1563 5781 6154 1563 Size: 4218 373 3063 Fragments arranged by size: 4218 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 4688 5717 7101 7369 1632 Size: 878 2178 1029 1384 268 1917 Fragments arranged by size: 2178 1917 1384 1029 878 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7457 AflIII A'CryG_T Cuts at: 1203 6553 7530 1203 Size: 5350 977 1327 Fragments arranged by size: 5350 1327 977 AgeI A'CCGG_T Cuts at: 6485 6485 Size: 7654 AhdI GACnn_n'nnGTC Cuts at: 2665 6383 2665 Size: 3718 3936 AleI CACnn'nnGTG Cuts at: 1166 2155 3182 4805 1166 Size: 989 1027 1623 4015 Fragments arranged by size: 4015 1623 1027 989 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7654 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2288 2320 2288 Size: 32 7622 AlwNI CAG_nnn'CTG Cuts at: 2671 3548 4334 5397 6969 2671 Size: 877 786 1063 1572 3356 Fragments arranged by size: 3356 1572 1063 877 786 ApaLI G'TGCA_C Cuts at: 5711 6867 5711 Size: 1156 6498 ApoI r'AATT_y Cuts at: 1493 2405 3896 4963 5189 5341 7450 1493 Size: 912 1491 1067 226 152 2109 1697 Fragments arranged by size: 2109 1697 1491 1067 912 226 152 AseI AT'TA_AT Cuts at: 160 1032 6208 160 Size: 872 5176 1606 Fragments arranged by size: 5176 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7654 AvaI C'yCGr_G Cuts at: 1870 1943 4979 1870 Size: 73 3036 4545 Fragments arranged by size: 4545 3036 73 BamHI G'GATC_C Cuts at: 4984 5351 4984 Size: 367 7287 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2650 4975 6430 618 Size: 325 217 627 863 2325 1455 1842 Fragments arranged by size: 2325 1842 1455 863 627 325 217 BanII G_rGCy'C Cuts at: 1391 2301 3263 3538 4973 1391 Size: 910 962 275 1435 4072 Fragments arranged by size: 4072 1435 962 910 275 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3819 928 Size: 971 1920 4763 Fragments arranged by size: 4763 1920 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5845 5879 1903 Size: 34 3908 34 3678 Fragments arranged by size: 3908 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5546 6756 5546 Size: 1210 6444 BclI T'GATC_A Cuts at: 1548 2392 4263 1548 Size: 844 1871 4939 Fragments arranged by size: 4939 1871 844 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6265 136 Size: 107 122 71 631 605 4593 1525 Fragments arranged by size: 4593 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7649 7649 Size: 7654 BlpI GC'TnA_GC Cuts at: 3257 5071 3257 Size: 1814 5840 Bme1580I G_kGCm'C Cuts at: 1790 2612 2876 5715 6871 1790 Size: 822 264 2839 1156 2573 Fragments arranged by size: 2839 2573 1156 822 264 BmgBI CAC'GTC Cuts at: 1318 4410 1318 Size: 3092 4562 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2269 2659 4147 Size: 416 53 83 399 24 852 390 1488 Cuts at: 4147 4489 4937 5385 6343 442 Size: 342 448 448 958 1753 Fragments arranged by size: 1753 1488 958 852 448 448 416 399 390 342 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7654 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3277 3277 Size: 7654 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3058 3334 4337 6314 1239 Size: 1819 276 1003 1977 2579 Fragments arranged by size: 2579 1977 1819 1003 276 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2285 3485 5779 6644 6942 2285 Size: 1200 2294 865 298 2997 Fragments arranged by size: 2997 2294 1200 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3011 3785 4233 4465 6317 882 Size: 617 1512 774 448 232 1852 2219 Fragments arranged by size: 2219 1852 1512 774 617 448 232 BsaAI yAC'GTr Cuts at: 493 1204 3520 4139 5445 493 Size: 711 2316 619 1306 2702 Fragments arranged by size: 2702 2316 1306 711 619 BsaBI GATnn'nnATC Cuts at: 2391 4364 7648 2391 Size: 1973 3284 2397 Fragments arranged by size: 3284 2397 1973 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5461 5843 6478 275 Size: 53 83 186 936 3928 382 635 1451 Fragments arranged by size: 3928 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4650 6087 6485 6759 6906 7317 4650 Size: 1437 398 274 147 411 4987 Fragments arranged by size: 4987 1437 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2288 2318 2962 2992 3587 Size: 30 165 30 885 30 644 30 595 Cuts at: 3587 3617 3691 3721 4442 4472 1178 Size: 30 74 30 721 30 4360 Fragments arranged by size: 4360 885 721 644 595 165 74 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2018 3659 4009 4365 1186 Size: 832 1641 350 356 4475 Fragments arranged by size: 4475 1641 832 356 350 BseYI C'CCAG_C Cuts at: 2128 4552 6857 7353 2128 Size: 2424 2305 496 2429 Fragments arranged by size: 2429 2424 2305 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3931 4704 1163 Size: 573 2195 773 4113 Fragments arranged by size: 4113 2195 773 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5104 5865 6893 1540 Size: 275 186 3103 761 1028 2301 Fragments arranged by size: 3103 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2301 3263 5715 5800 6871 2301 Size: 962 2452 85 1071 3084 Fragments arranged by size: 3084 2452 1071 962 85 BsmI GAATG_Cn' Cuts at: 2328 5165 5258 2328 Size: 2837 93 4724 Fragments arranged by size: 4724 2837 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 2612 2876 3263 3538 4973 5715 Size: 399 511 311 264 387 275 1435 742 Cuts at: 5715 5800 6871 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1435 1071 742 511 399 387 311 275 264 85 BspEI T'CCGG_A Cuts at: 4650 7317 4650 Size: 2667 4987 BspHI T'CATG_A Cuts at: 3633 3832 5543 7273 3633 Size: 199 1711 1730 4014 Fragments arranged by size: 4014 1730 1711 199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2907 3881 5009 844 Size: 2063 974 1128 3489 Fragments arranged by size: 3489 2063 1128 974 BsrBI CCG'CTC Cuts at: 1394 1823 5541 1394 Size: 429 3718 3507 Fragments arranged by size: 3718 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2224 2439 2788 3763 4729 6149 6323 Size: 1183 976 215 349 975 966 1420 174 Cuts at: 6323 65 Size: 1396 Fragments arranged by size: 1420 1396 1183 976 975 966 349 215 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6298 6485 1700 Size: 110 4488 187 2869 Fragments arranged by size: 4488 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7654 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7654 BssSI C'ACGA_G Cuts at: 5714 6726 5714 Size: 1012 6642 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7654 BstBI TT'CG_AA Cuts at: 6458 6458 Size: 7654 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7654 BstXI CCAn_nnnn'nTGG Cuts at: 3850 4371 3850 Size: 521 7133 BstYI r'GATC_y Cuts at: 1972 2136 2470 2716 4359 4984 5351 5736 5753 Size: 164 334 246 1643 625 367 385 17 Cuts at: 5753 7205 7291 7303 7649 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1643 1452 625 385 367 346 334 246 164 86 17 12 BstZ17I GTA'TAC Cuts at: 2596 2596 Size: 7654 BtgI C'CryG_G Cuts at: 513 1065 2004 2655 2961 3180 4542 4875 4950 Size: 552 939 651 306 219 1362 333 75 Cuts at: 4950 513 Size: 3217 Fragments arranged by size: 3217 1362 939 651 552 333 306 219 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4434 487 Size: 683 56 3208 3707 Fragments arranged by size: 3707 3208 683 56 BtsI GCAGTG_nn' Cuts at: 3919 4783 5170 5964 5984 3919 Size: 864 387 794 20 5589 Fragments arranged by size: 5589 864 794 387 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7654 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7619 DraI TTT'AAA Cuts at: 3768 4958 3768 Size: 1190 6464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3416 6661 809 Size: 507 2100 3245 1802 Fragments arranged by size: 3245 2100 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3954 5101 5990 Size: 54 1425 50 275 18 2124 1147 889 Cuts at: 5990 7619 8 Size: 1629 43 Fragments arranged by size: 2124 1629 1425 1147 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5101 1537 Size: 275 3289 4090 Fragments arranged by size: 4090 3289 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2157 5584 1353 Size: 804 3427 3423 Fragments arranged by size: 3427 3423 804 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6241 6615 6761 387 Size: 1064 138 4652 374 146 1280 Fragments arranged by size: 4652 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2299 3261 2299 Size: 962 6692 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2510 3058 3334 4337 4688 5717 6314 Size: 393 878 548 276 1003 351 1029 597 Cuts at: 6314 7101 7369 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1029 1003 878 787 597 548 393 351 276 268 EcoNI CCTnn'n_nnAGG Cuts at: 2675 2675 Size: 7654 EcoO109I rG'GnC_Cy Cuts at: 2263 2519 2263 Size: 256 7398 EcoRI G'AATT_C Cuts at: 4963 5341 4963 Size: 378 7276 EcoRV GAT'ATC Cuts at: 4732 4732 Size: 7654 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4813 4813 Size: 7654 FspI TGC'GCA Cuts at: 6160 6160 Size: 7654 HaeII r_GCGC'y Cuts at: 1678 6801 1678 Size: 5123 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2128 2160 2651 2683 2942 Size: 32 135 32 556 32 491 32 259 Cuts at: 2942 2974 3332 3364 3587 3619 4344 4376 4442 Size: 32 358 32 223 32 725 32 66 Cuts at: 4442 4474 4477 4509 6295 6327 6369 6401 1373 Size: 32 3 32 1786 32 42 32 2626 Fragments arranged by size: 2626 1786 725 556 491 358 259 223 135 66 42 32 32 32 32 32 32 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 669 1657 1938 2527 2698 2825 3796 4998 5244 Size: 988 281 589 171 127 971 1202 246 Cuts at: 5244 669 Size: 3079 Fragments arranged by size: 3079 1202 988 971 589 281 246 171 127 HindIII A'AGCT_T Cuts at: 748 2817 4643 748 Size: 2069 1826 3759 Fragments arranged by size: 3759 2069 1826 HpaI GTT'AAC Cuts at: 5244 5244 Size: 7654 KpnI G_GTAC'C Cuts at: 2654 4979 2654 Size: 2325 5329 MfeI C'AATT_G Cuts at: 2242 5253 2242 Size: 3011 4643 MluI A'CGCG_T Cuts at: 7530 7530 Size: 7654 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3865 6768 6952 1305 Size: 2560 2903 184 2007 Fragments arranged by size: 2903 2560 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3956 10 Size: 54 1425 343 2124 3708 Fragments arranged by size: 3708 2124 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2155 2465 3182 3393 3807 4805 5612 Size: 648 989 310 717 211 414 998 807 Cuts at: 5612 5971 6130 518 Size: 359 159 2042 Fragments arranged by size: 2042 998 989 807 717 648 414 359 310 211 159 MspA1I CmG'CkG Cuts at: 1606 2040 2447 3548 3650 4556 4570 5070 5747 Size: 434 407 1101 102 906 14 500 677 Cuts at: 5747 6895 7140 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1101 906 677 500 434 407 245 102 14 NcoI C'CATG_G Cuts at: 513 1065 2004 2655 2961 4542 513 Size: 552 939 651 306 1581 3625 Fragments arranged by size: 3625 1581 939 651 552 306 NdeI CA'TA_TG Cuts at: 387 3089 3808 387 Size: 2702 719 4233 Fragments arranged by size: 4233 2702 719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7654 NotI GC'GGCC_GC Cuts at: 5101 5101 Size: 7654 NspI r_CATG'y Cuts at: 1132 1229 5012 6557 1132 Size: 97 3783 1545 2229 Fragments arranged by size: 3783 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7654 PciI A'CATG_T Cuts at: 6553 6553 Size: 7654 PflMI CCAn_nnn'nTGG Cuts at: 1108 2153 2671 1108 Size: 1045 518 6091 Fragments arranged by size: 6091 1045 518 PfoI T'CCnGG_A Cuts at: 3030 4314 3030 Size: 1284 6370 PmeI GTTT'AAAC Cuts at: 4958 4958 Size: 7654 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2288 2320 5723 5755 7236 7268 2288 Size: 32 3403 32 1481 32 2674 Fragments arranged by size: 3403 2674 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2263 2519 2263 Size: 256 7398 PshAI GACnn'nnGTC Cuts at: 1613 2232 1613 Size: 619 7035 PsiI TTA'TAA Cuts at: 5224 5224 Size: 7654 PstI C_TGCA'G Cuts at: 830 2738 2918 5006 830 Size: 1908 180 2088 3478 Fragments arranged by size: 3478 2088 1908 180 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7654 PvuII CAG'CTG Cuts at: 2040 2447 4556 4570 2040 Size: 407 2109 14 5124 Fragments arranged by size: 5124 2109 407 14 SacI G_AGCT'C Cuts at: 2301 3263 2301 Size: 962 6692 SalI G'TCGA_C Cuts at: 1936 2823 4996 1936 Size: 887 2173 4594 Fragments arranged by size: 4594 2173 887 SanDI GG'GwC_CC Cuts at: 2263 2263 Size: 7654 SbfI CC_TGCA'GG Cuts at: 5006 5006 Size: 7654 ScaI AGT'ACT Cuts at: 5902 7614 5902 Size: 1712 5942 SexAI A'CCwGG_T Cuts at: 2208 2827 2208 Size: 619 7035 SfcI C'TryA_G Cuts at: 826 1045 2734 2914 4389 4512 5002 6137 6818 Size: 219 1689 180 1475 123 490 1135 681 Cuts at: 6818 7009 826 Size: 191 1471 Fragments arranged by size: 1689 1475 1471 1135 681 490 219 191 180 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7654 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7654 SmaI CCC'GGG Cuts at: 1872 4981 1872 Size: 3109 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2300 3500 5758 6659 6921 820 Size: 197 926 357 1200 2258 901 262 1553 Fragments arranged by size: 2258 1553 1200 926 901 357 262 197 SnaBI TAC'GTA Cuts at: 493 3520 4139 5445 493 Size: 3027 619 1306 2702 Fragments arranged by size: 3027 2702 1306 619 SpeI A'CTAG_T Cuts at: 152 3587 152 Size: 3435 4219 SphI G_CATG'C Cuts at: 1132 5012 1132 Size: 3880 3774 SspI AAT'ATT Cuts at: 5 52 5578 5 Size: 47 5526 2081 Fragments arranged by size: 5526 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7654 StyI C'CwwG_G Cuts at: 513 1065 2004 2577 2655 2723 2961 3843 4122 Size: 552 939 573 78 68 238 882 279 Cuts at: 4122 4542 5093 513 Size: 420 551 3074 Fragments arranged by size: 3074 939 882 573 552 551 420 279 238 78 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2145 2209 3093 5681 5698 5851 6036 Size: 1255 565 64 884 2588 17 153 185 Cuts at: 6036 6481 325 Size: 445 1498 Fragments arranged by size: 2588 1498 1255 884 565 445 185 153 64 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2370 2730 2738 Size: 274 80 33 51 1443 393 360 8 Cuts at: 2738 4812 5900 7612 96 Size: 2074 1088 1712 138 Fragments arranged by size: 2074 1712 1443 1088 393 360 274 138 80 51 33 8 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3691 3723 7614 7646 3691 Size: 32 3891 32 3699 Fragments arranged by size: 3891 3699 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7654 XbaI T'CTAG_A Cuts at: 4918 4990 4918 Size: 72 7582 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2154 2154 Size: 7654 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7654 XmaI C'CCGG_G Cuts at: 1870 4979 1870 Size: 3109 4545 XmnI GAAnn'nnTTC Cuts at: 2070 5783 2070 Size: 3713 3941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5462 276 Size: 53 83 186 936 3928 2468 Fragments arranged by size: 3928 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI