Restriction map for
pFN21AB0038
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 541 GGTACC G^GTACC
AccIII 305 TCCGGA T^CCGGA
AocI 641 CCTTAGG CC^T[ACGT]AGG
Asp718I 541 GGTACC G^GTACC
BalI 1016 TGGCCA TGG^CCA
BbsI 755 GAAGAC GAAGAC(2/6)
BbvCI 805 GCTGAGG CCTCAGC(-5/-2)
BlpI 800 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 800 GCTCAGC GC^T[ACGT]AGC
BpuAI 755 GAAGAC GAAGAC(2/6)
BsaI 455 GAGACC GGTCTC(1/5)
BseAI 305 TCCGGA T^CCGGA
BseRI 238 CTCCTC GAGGAG(10/8)
BsgI 775 GTGCAG GTGCAG(16/14)
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsmAI 455 GAGAC GTCTC(1/5)
BspEI 305 TCCGGA T^CCGGA
BspMI 421 ACCTGC ACCTGC(4/8)
BsrI 822 ACTGG ACTGG(1/-1)
BssSI 85 CTCGTG CACGAG(-5/-1)
BstAPI 416 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 641 CCTTAGG CC^T[ACGT]AGG
CelII 800 GCTCAGC GC^T[ACGT]AGC
DraI 1153 TTTAAA TTT^AAA
DraIII 733 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 812 CCACGG C^C[GA][CT]GG
EagI 35 CGGCCG C^GGCCG
EarI 1042 GAAGAG CTCTTC(1/4)
EclXI 35 CGGCCG C^GGCCG
Eco52I 35 CGGCCG C^GGCCG
Eco81I 641 CCTTAGG CC^T[ACGT]AGG
HaeII 655 AGCGCC [GA]GCGC^[CT]
HgaI 71 GACGC GACGC(5/10)
KpnI 541 GGTACC GGTAC^C
Ksp632I 1042 GAAGAG CTCTTC(1/4)
MluNI 1016 TGGCCA TGG^CCA
MroI 305 TCCGGA T^CCGGA
MscI 1016 TGGCCA TGG^CCA
MslI 1089 CACGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 333 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 770 ACCTGGT A^CC[AT]GGT
SmlI 676 CTCAAG C^T[CT][GA]AG
TfiI 1022 GAATC G^A[AT]TC
Van91I 333 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 33 CGCG CG^CG
427 CGCG
AlwNI 334 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
417 CAGCACCTG
ApaI 356 GGGCCC GGGCC^C
974 GGGCCC
AspHI 193 GAGCTC G[AT]GC[AT]^C
616 GAGCTC
BamHI 308 GGATCC G^GATCC
1007 GGATCC
BfaI 797 CTAG C^TAG
898 CTAG
BsaWI 129 ACCGGA [AT]^CCGG[AT]
305 TCCGGA
BsiHKAI 193 GAGCTC G[AT]GC[AT]^C
616 GAGCTC
BsrFI 658 GCCGGC [GA]^CCGG[CT]
727 GCCGGC
BstBI 281 TTCGAA TT^CGAA
371 TTCGAA
BstUI 33 CGCG CG^CG
427 CGCG
Cfr10I 658 GCCGGC [GA]^CCGG[CT]
727 GCCGGC
EcoNI 629 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1071 CCTGGAGAAGG
EcoT14I 254 CCAAGG C^C[AT][AT]GG
1143 CCAAGG
MaeI 797 CTAG C^TAG
898 CTAG
MaeII 519 ACGT A^CGT
1094 ACGT
MseI 1124 TTAA T^TAA
1154 TTAA
MvnI 33 CGCG CG^CG
427 CGCG
NaeI 658 GCCGGC GCC^GGC
727 GCCGGC
NgoMIV 658 GCCGGC G^CCGGC
727 GCCGGC
NspV 281 TTCGAA TT^CGAA
371 TTCGAA
PpuMI 335 AGGACCT [GA]G^G[AT]CC[CT]
1031 GGGACCC
PvuII 745 CAGCTG CAG^CTG
803 CAGCTG
SacI 193 GAGCTC GAGCT^C
616 GAGCTC
SfaNI 148 GATGC GCATC(5/9)
302 GCATC
SfuI 281 TTCGAA TT^CGAA
371 TTCGAA
StyI 254 CCAAGG C^C[AT][AT]GG
1143 CCAAGG
Tru9I 1124 TTAA T^TAA
1154 TTAA
TspRI 735 CAGTG CA[GC]TG[ACGT][ACGT]^
778 CAGTG
XcmI 812 CCACGGGCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1004 CCAGGATCCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrBI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SmaI TaqI TseI
Tsp45I TthHB8I XhoII XmaCI XmaI