(Circular) (Six-base) MAPSORT of: pFN21AB0038.seq Check: 9529 from: 1 to: 5872 pFN21AB0038 5872 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2435 2435 Size: 5872 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3215 1937 Size: 1278 4594 Acc65I G'GTAC_C Cuts at: 2546 3193 2546 Size: 647 5225 AclI AA'CG_TT Cuts at: 1563 3999 4372 1563 Size: 2436 373 3063 Fragments arranged by size: 3063 2436 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 3935 5319 5587 1632 Size: 443 1860 1384 268 1917 Fragments arranged by size: 1917 1860 1384 443 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 4771 5748 1203 Size: 3568 977 1327 Fragments arranged by size: 3568 1327 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 3098 1166 Size: 1932 3940 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2886 1184 Size: 1702 4170 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2018 2050 2018 Size: 32 5840 AlwNI CAG_nnn'CTG Cuts at: 2344 2427 3615 5187 2344 Size: 83 1188 1572 3029 Fragments arranged by size: 3029 1572 1188 83 ApaI G_GGCC'C Cuts at: 2365 2983 2365 Size: 618 5254 ApaLI G'TGCA_C Cuts at: 3929 5085 3929 Size: 1156 4716 ApoI r'AATT_y Cuts at: 1493 3181 3407 3559 5668 1493 Size: 1688 226 152 2109 1697 Fragments arranged by size: 2109 1697 1688 226 152 AseI AT'TA_AT Cuts at: 160 1032 4426 160 Size: 872 3394 1606 Fragments arranged by size: 3394 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 2358 2364 2445 2919 3197 1870 Size: 73 415 6 81 474 278 4545 Fragments arranged by size: 4545 474 415 278 81 73 6 BamHI G'GATC_C Cuts at: 2313 3012 3202 3569 2313 Size: 699 190 367 4616 Fragments arranged by size: 4616 699 367 190 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2546 2897 2915 3193 4648 Size: 325 217 627 759 351 18 278 1455 Cuts at: 4648 618 Size: 1842 Fragments arranged by size: 1842 1455 759 627 351 325 278 217 18 BanII G_rGCy'C Cuts at: 1391 2202 2365 2625 2983 3062 3191 1391 Size: 811 163 260 358 79 129 4072 Fragments arranged by size: 4072 811 358 260 163 129 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2767 928 Size: 971 868 4033 Fragments arranged by size: 4033 971 868 BbvCI CC'TCA_GC Cuts at: 2811 2811 Size: 5872 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4063 4097 1903 Size: 34 2126 34 3678 Fragments arranged by size: 3678 2126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 1548 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4483 136 Size: 107 122 71 631 605 2811 1525 Fragments arranged by size: 2811 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5867 5867 Size: 5872 BlpI GC'TnA_GC Cuts at: 2806 3289 2806 Size: 483 5389 Bme1580I G_kGCm'C Cuts at: 1790 2365 2902 2983 3933 5089 1790 Size: 575 537 81 950 1156 2573 Fragments arranged by size: 2573 1156 950 575 537 81 BmgBI CAC'GTC Cuts at: 1318 3100 1318 Size: 1782 4090 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3603 4561 442 Size: 416 53 83 399 24 2186 958 1753 Fragments arranged by size: 2186 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5872 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2696 2696 Size: 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2900 3076 3098 4532 1239 Size: 1661 176 22 1434 2579 Fragments arranged by size: 2579 1661 1434 176 22 Bpu10I CC'TnA_GC Cuts at: 2811 2935 2811 Size: 124 5748 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2666 3997 4862 5160 2666 Size: 1331 865 298 3378 Fragments arranged by size: 3378 1331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2454 4535 882 Size: 617 955 2081 2219 Fragments arranged by size: 2219 2081 955 617 BsaAI yAC'GTr Cuts at: 493 1204 3663 493 Size: 711 2459 2702 Fragments arranged by size: 2702 2459 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3679 4061 4696 275 Size: 53 83 186 936 2146 382 635 1451 Fragments arranged by size: 2146 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2134 2310 4305 4703 4977 5124 5535 2134 Size: 176 1995 398 274 147 411 2471 Fragments arranged by size: 2471 1995 411 398 274 176 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2018 2048 2333 2363 3035 Size: 30 165 30 615 30 285 30 672 Cuts at: 3035 3065 1178 Size: 30 3985 Fragments arranged by size: 3985 672 615 285 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2234 1186 Size: 1048 4824 BseYI C'CCAG_C Cuts at: 2570 2657 2945 5075 5571 2570 Size: 87 288 2130 496 2871 Fragments arranged by size: 2871 2130 496 288 87 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2801 1163 Size: 573 1065 4234 Fragments arranged by size: 4234 1065 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 3322 4083 5111 1540 Size: 275 186 42 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 42 BsiHKAI G_wGCw'C Cuts at: 2202 2625 3933 4018 5089 2202 Size: 423 1308 85 1071 2985 Fragments arranged by size: 2985 1308 1071 423 85 BsmI GAATG_Cn' Cuts at: 3383 3476 3383 Size: 93 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2365 2625 2902 2983 3062 3191 Size: 399 412 163 260 277 81 79 129 Cuts at: 3191 3933 4018 5089 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 412 399 277 260 163 129 85 81 79 BspEI T'CCGG_A Cuts at: 2310 5535 2310 Size: 3225 2647 BspHI T'CATG_A Cuts at: 3761 5491 3761 Size: 1730 4142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2435 3227 844 Size: 1591 792 3489 Fragments arranged by size: 3489 1591 792 BsrBI CCG'CTC Cuts at: 1394 1823 2029 2331 2482 2518 2542 3759 1394 Size: 429 206 302 151 36 24 1217 3507 Fragments arranged by size: 3507 1217 429 302 206 151 36 24 BsrDI GCAATG_nn' Cuts at: 65 1248 4367 4541 65 Size: 1183 3119 174 1396 Fragments arranged by size: 3119 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2663 2732 4516 4703 1700 Size: 110 853 69 1784 187 2869 Fragments arranged by size: 2869 1784 853 187 110 69 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5872 BssSI C'ACGA_G Cuts at: 2090 3932 4944 2090 Size: 1842 1012 3018 Fragments arranged by size: 3018 1842 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2427 794 Size: 1633 4239 BstBI TT'CG_AA Cuts at: 2287 2377 4676 2287 Size: 90 2299 3483 Fragments arranged by size: 3483 2299 90 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5872 BstXI CCAn_nnnn'nTGG Cuts at: 2557 2953 3031 2557 Size: 396 78 5398 Fragments arranged by size: 5398 396 78 BstYI r'GATC_y Cuts at: 1972 2313 2905 3012 3202 3569 3954 3971 5423 Size: 341 592 107 190 367 385 17 1452 Cuts at: 5423 5509 5521 5867 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 592 385 367 346 341 190 107 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2647 2647 Size: 5872 BtgI C'CryG_G Cuts at: 513 1065 2004 2817 513 Size: 552 939 813 3568 Fragments arranged by size: 3568 939 813 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2989 487 Size: 683 56 1763 3370 Fragments arranged by size: 3370 1763 683 56 BtsI GCAGTG_nn' Cuts at: 2789 3388 4182 4202 2789 Size: 599 794 20 4459 Fragments arranged by size: 4459 794 599 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2200 2235 529 Size: 35 1636 35 4166 Fragments arranged by size: 4166 1636 35 35 DraI TTT'AAA Cuts at: 3160 3176 3160 Size: 16 5856 DraIII CAC_nnn'GTG Cuts at: 2743 2743 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4879 809 Size: 507 3563 1802 Fragments arranged by size: 3563 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2326 2734 Size: 54 1425 50 275 18 210 286 408 Cuts at: 2734 2847 3021 3319 4208 5837 8 Size: 113 174 298 889 1629 43 Fragments arranged by size: 1629 1425 889 408 298 286 275 210 174 113 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2040 3319 1537 Size: 275 228 1279 4090 Fragments arranged by size: 4090 1279 275 228 EarI CTCTTCn'nnn_ Cuts at: 1353 3042 3802 1353 Size: 1689 760 3423 Fragments arranged by size: 3423 1689 760 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4459 4833 4979 387 Size: 1064 138 2870 374 146 1280 Fragments arranged by size: 2870 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2200 2623 2200 Size: 423 5449 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2900 3076 3098 3935 4532 5319 Size: 393 443 825 176 22 837 597 787 Cuts at: 5319 5587 1239 Size: 268 1524 Fragments arranged by size: 1524 837 825 787 597 443 393 268 176 22 EcoNI CCTnn'n_nnAGG Cuts at: 2638 3080 2638 Size: 442 5430 EcoO109I rG'GnC_Cy Cuts at: 2341 2371 2440 2603 2643 2939 2959 2979 2994 Size: 30 69 163 40 296 20 20 15 Cuts at: 2994 3037 2341 Size: 43 5176 Fragments arranged by size: 5176 296 163 69 43 40 30 20 20 15 EcoRI G'AATT_C Cuts at: 3181 3559 3181 Size: 378 5494 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 2664 5019 1678 Size: 986 2355 2531 Fragments arranged by size: 2531 2355 986 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2333 2365 3035 3067 3089 Size: 32 135 32 761 32 670 32 22 Cuts at: 3089 3121 4513 4545 4587 4619 1373 Size: 32 1392 32 42 32 2626 Fragments arranged by size: 2626 1392 761 670 135 42 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3216 3462 669 Size: 988 281 1278 246 3079 Fragments arranged by size: 3079 1278 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5872 HpaI GTT'AAC Cuts at: 3462 3462 Size: 5872 KpnI G_GTAC'C Cuts at: 2550 3197 2550 Size: 647 5225 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2950 4986 5170 1305 Size: 1645 2036 184 2007 Fragments arranged by size: 2036 2007 1645 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3023 10 Size: 54 1425 343 1191 2859 Fragments arranged by size: 2859 1425 1191 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3098 3830 4189 4348 518 Size: 648 1932 732 359 159 2042 Fragments arranged by size: 2042 1932 732 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2752 2810 3288 3965 5113 5358 1606 Size: 412 734 58 478 677 1148 245 2120 Fragments arranged by size: 2120 1148 734 677 478 412 245 58 NaeI GCC'GGC Cuts at: 2665 2734 2665 Size: 69 5803 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4381 Fragments arranged by size: 4381 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5872 NgoMIV G'CCGG_C Cuts at: 2663 2732 2663 Size: 69 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5872 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 3230 4775 1132 Size: 97 2001 1545 2229 Fragments arranged by size: 2229 2001 1545 97 PasI CC'CwG_GG Cuts at: 1348 2983 1348 Size: 1635 4237 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 1108 Size: 1236 4636 PfoI T'CCnGG_A Cuts at: 2148 2625 2877 3053 2148 Size: 477 252 176 4967 Fragments arranged by size: 4967 477 252 176 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2018 2050 3941 3973 5454 5486 2018 Size: 32 1891 32 1481 32 2404 Fragments arranged by size: 2404 1891 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2341 3037 2341 Size: 696 5176 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5872 PsiI TTA'TAA Cuts at: 3442 3442 Size: 5872 PspOMI G'GGCC_C Cuts at: 2361 2979 2361 Size: 618 5254 PstI C_TGCA'G Cuts at: 830 3224 830 Size: 2394 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 PvuII CAG'CTG Cuts at: 2752 2810 2752 Size: 58 5814 SacI G_AGCT'C Cuts at: 2202 2625 2202 Size: 423 5449 SalI G'TCGA_C Cuts at: 1936 3214 1936 Size: 1278 4594 SanDI GG'GwC_CC Cuts at: 3037 3037 Size: 5872 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 4120 5832 4120 Size: 1712 4160 SexAI A'CCwGG_T Cuts at: 2775 2775 Size: 5872 SfcI C'TryA_G Cuts at: 826 1045 3220 4355 5036 5227 826 Size: 219 2175 1135 681 191 1471 Fragments arranged by size: 2175 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 2360 2366 2921 3199 1872 Size: 488 6 555 278 4545 Fragments arranged by size: 4545 555 488 278 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2681 3976 4877 5139 820 Size: 197 926 738 1295 901 262 1553 Fragments arranged by size: 1553 1295 926 901 738 262 197 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 3230 1132 Size: 2098 3774 SspI AAT'ATT Cuts at: 5 52 3796 5 Size: 47 3744 2081 Fragments arranged by size: 3744 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5872 StyI C'CwwG_G Cuts at: 513 1065 2004 2259 3148 3311 513 Size: 552 939 255 889 163 3074 Fragments arranged by size: 3074 939 889 552 255 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2472 3899 3916 4069 4254 4699 325 Size: 1255 892 1427 17 153 185 445 1498 Fragments arranged by size: 1498 1427 1255 892 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 4118 5830 Size: 274 80 33 51 1443 141 2000 1712 Cuts at: 5830 96 Size: 138 Fragments arranged by size: 2000 1712 1443 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5832 5864 5832 Size: 32 5840 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5872 XbaI T'CTAG_A Cuts at: 3208 3208 Size: 5872 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2824 3016 2824 Size: 192 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 2358 2364 2919 3197 1870 Size: 488 6 555 278 4545 Fragments arranged by size: 4545 555 488 278 6 XmnI GAAnn'nnTTC Cuts at: 4001 4001 Size: 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI