Restriction map for
pFN21AB0038
(Linear) (Six-base) MAPSORT of: KIBB0038.seq Check: 9302 from: 1 to: 1167
KIBB0038.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 430 1167
Size: 430 737
Acc65I G'GTAC_C
Cuts at: 0 541 1167
Size: 541 626
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1167
Size: 70 1097
AleI CACnn'nnGTG
Cuts at: 0 1093 1167
Size: 1093 74
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 881 1167
Size: 881 286
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 13 45 1167
Size: 13 32 1122
Fragments arranged by size:
1122 32 13
AlwNI CAG_nnn'CTG
Cuts at: 0 339 422 1167
Size: 339 83 745
Fragments arranged by size:
745 339 83
ApaI G_GGCC'C
Cuts at: 0 360 978 1167
Size: 360 618 189
Fragments arranged by size:
618 360 189
AvaI C'yCGr_G
Cuts at: 0 353 359 440 914 1167
Size: 353 6 81 474 253
Fragments arranged by size:
474 353 253 81 6
BamHI G'GATC_C
Cuts at: 0 308 1007 1167
Size: 308 699 160
Fragments arranged by size:
699 308 160
BanI G'GyrC_C
Cuts at: 0 541 892 910 1167
Size: 541 351 18 257
Fragments arranged by size:
541 351 257 18
BanII G_rGCy'C
Cuts at: 0 197 360 620 978 1057 1167
Size: 197 163 260 358 79 110
Fragments arranged by size:
358 260 197 163 110 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 762 1167
Size: 762 405
BbvCI CC'TCA_GC
Cuts at: 0 806 1167
Size: 806 361
BlpI GC'TnA_GC
Cuts at: 0 801 1167
Size: 801 366
Bme1580I G_kGCm'C
Cuts at: 0 360 897 978 1167
Size: 360 537 81 189
Fragments arranged by size:
537 360 189 81
BmgBI CAC'GTC
Cuts at: 0 1095 1167
Size: 1095 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 691 1167
Size: 691 476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 1071 1093 1167
Size: 895 176 22 74
Fragments arranged by size:
895 176 74 22
Bpu10I CC'TnA_GC
Cuts at: 0 806 930 1167
Size: 806 124 237
Fragments arranged by size:
806 237 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1167
Size: 661 506
BsaI GGTCTCn'nnnn_
Cuts at: 0 449 1167
Size: 449 718
BsaWI w'CCGG_w
Cuts at: 0 129 305 1167
Size: 129 176 862
Fragments arranged by size:
862 176 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 13 43 328 358 1030 1060 1167
Size: 13 30 285 30 672 30 107
Fragments arranged by size:
672 285 107 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 1167
Size: 229 938
BseYI C'CCAG_C
Cuts at: 0 565 652 940 1167
Size: 565 87 288 227
Fragments arranged by size:
565 288 227 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1167
Size: 796 371
BsiEI CG_ry'CG
Cuts at: 0 38 1167
Size: 38 1129
BsiHKAI G_wGCw'C
Cuts at: 0 197 620 1167
Size: 197 423 547
Fragments arranged by size:
547 423 197
Bsp1286I G_dGCh'C
Cuts at: 0 197 360 620 897 978 1057 1167
Size: 197 163 260 277 81 79 110
Fragments arranged by size:
277 260 197 163 110 81 79
BspEI T'CCGG_A
Cuts at: 0 305 1167
Size: 305 862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 430 1167
Size: 430 737
BsrBI CCG'CTC
Cuts at: 0 24 326 477 513 537 1167
Size: 24 302 151 36 24 630
Fragments arranged by size:
630 302 151 36 24 24
BsrFI r'CCGG_y
Cuts at: 0 658 727 1167
Size: 658 69 440
Fragments arranged by size:
658 440 69
BssSI C'ACGA_G
Cuts at: 0 85 1167
Size: 85 1082
BstAPI GCAn_nnn'nTGC
Cuts at: 0 422 1167
Size: 422 745
BstBI TT'CG_AA
Cuts at: 0 282 372 1167
Size: 282 90 795
Fragments arranged by size:
795 282 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 552 948 1026 1167
Size: 552 396 78 141
Fragments arranged by size:
552 396 141 78
BstYI r'GATC_y
Cuts at: 0 308 900 1007 1167
Size: 308 592 107 160
Fragments arranged by size:
592 308 160 107
Bsu36I CC'TnA_GG
Cuts at: 0 642 1167
Size: 642 525
BtgI C'CryG_G
Cuts at: 0 812 1167
Size: 812 355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 984 1167
Size: 984 183
BtsI GCAGTG_nn'
Cuts at: 0 784 1167
Size: 784 383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 195 230 1167
Size: 195 35 937
Fragments arranged by size:
937 195 35
DraI TTT'AAA
Cuts at: 0 1155 1167
Size: 1155 12
DraIII CAC_nnn'GTG
Cuts at: 0 738 1167
Size: 738 429
EaeI y'GGCC_r
Cuts at: 0 35 321 729 842 1016 1167
Size: 35 286 408 113 174 151
Fragments arranged by size:
408 286 174 151 113 35
EagI C'GGCC_G
Cuts at: 0 35 1167
Size: 35 1132
EarI CTCTTCn'nnn_
Cuts at: 0 1037 1167
Size: 1037 130
EcoICRI GAG'CTC
Cuts at: 0 195 618 1167
Size: 195 423 549
Fragments arranged by size:
549 423 195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 895 1071 1093 1167
Size: 70 825 176 22 74
Fragments arranged by size:
825 176 74 70 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 633 1075 1167
Size: 633 442 92
Fragments arranged by size:
633 442 92
EcoO109I rG'GnC_Cy
Cuts at: 0 336 366 435 598 638 934 954 974
Size: 336 30 69 163 40 296 20 20
Cuts at: 974 989 1032 1167
Size: 15 43 135
Fragments arranged by size:
336 296 163 135 69 43 40 30
20 20 15
HaeII r_GCGC'y
Cuts at: 0 659 1167
Size: 659 508
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 328 360 1030 1062 1084 1116 1167
Size: 328 32 670 32 22 32 51
Fragments arranged by size:
670 328 51 32 32 32 22
KpnI G_GTAC'C
Cuts at: 0 545 1167
Size: 545 622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1167
Size: 945 222
MscI TGG'CCA
Cuts at: 0 1018 1167
Size: 1018 149
MslI CAynn'nnrTG
Cuts at: 0 1093 1167
Size: 1093 74
MspA1I CmG'CkG
Cuts at: 0 13 747 805 1167
Size: 13 734 58 362
Fragments arranged by size:
734 362 58 13
NaeI GCC'GGC
Cuts at: 0 660 729 1167
Size: 660 69 438
Fragments arranged by size:
660 438 69
NgoMIV G'CCGG_C
Cuts at: 0 658 727 1167
Size: 658 69 440
Fragments arranged by size:
658 440 69
PasI CC'CwG_GG
Cuts at: 0 978 1167
Size: 978 189
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 1167
Size: 339 828
PfoI T'CCnGG_A
Cuts at: 0 143 620 872 1048 1167
Size: 143 477 252 176 119
Fragments arranged by size:
477 252 176 143 119
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 1167
Size: 13 32 1122
Fragments arranged by size:
1122 32 13
PpuMI rG'GwC_Cy
Cuts at: 0 336 1032 1167
Size: 336 696 135
Fragments arranged by size:
696 336 135
PspOMI G'GGCC_C
Cuts at: 0 356 974 1167
Size: 356 618 193
Fragments arranged by size:
618 356 193
PvuII CAG'CTG
Cuts at: 0 747 805 1167
Size: 747 58 362
Fragments arranged by size:
747 362 58
SacI G_AGCT'C
Cuts at: 0 197 620 1167
Size: 197 423 547
Fragments arranged by size:
547 423 197
SanDI GG'GwC_CC
Cuts at: 0 1032 1167
Size: 1032 135
SexAI A'CCwGG_T
Cuts at: 0 770 1167
Size: 770 397
SmaI CCC'GGG
Cuts at: 0 355 361 916 1167
Size: 355 6 555 251
Fragments arranged by size:
555 355 251 6
SmlI C'TyrA_G
Cuts at: 0 676 1167
Size: 676 491
StyI C'CwwG_G
Cuts at: 0 254 1143 1167
Size: 254 889 24
Fragments arranged by size:
889 254 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 467 1167
Size: 467 700
TatI w'GTAC_w
Cuts at: 0 113 1167
Size: 113 1054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 819 1011 1167
Size: 819 192 156
Fragments arranged by size:
819 192 156
XmaI C'CCGG_G
Cuts at: 0 353 359 914 1167
Size: 353 6 555 253
Fragments arranged by size:
555 353 253 6
Enzymes that do cut:
AarI Acc65I AcuI AleI AlfI AloI AlwNI ApaI
AvaI BamHI BanI BanII BbsI BbvCI BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NaeI NgoMIV PasI PflMI
PfoI PpiI PpuMI PspOMI PvuII SacI SanDI SexAI
SmaI SmlI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BciVI BclI BfrBI BglI BglII BmrI BmtI
BsaAI BsaBI BsaHI BsiWI BsmI BsmBI BspHI BsrDI
BsrGI BssHII BstEII BstZ17I ClaI DrdI EciI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmnI ZraI