Restriction map for
pFN21AB0016
(Linear) (Six-base) MAPSORT of: KIBB0016.seq Check: 7571 from: 1 to: 5070
KIBB0016.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AatII G_ACGT'C
Cuts at: 0 1788 5070
Size: 1788 3282
AccI GT'mk_AC
Cuts at: 0 1081 4868 5070
Size: 1081 3787 202
Fragments arranged by size:
3787 1081 202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 700 727 1072 1763 1949 2701 3682
Size: 334 366 27 345 691 186 752 981
Cuts at: 3682 3743 4448 4487 5070
Size: 61 705 39 583
Fragments arranged by size:
981 752 705 691 583 366 345 334
186 61 39 27
AflII C'TTAA_G
Cuts at: 0 3184 5070
Size: 3184 1886
AflIII A'CryG_T
Cuts at: 0 657 2044 2065 4166 5070
Size: 657 1387 21 2101 904
Fragments arranged by size:
2101 1387 904 657 21
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4857 5070
Size: 4857 213
AlwNI CAG_nnn'CTG
Cuts at: 0 228 679 2854 3219 4033 5070
Size: 228 451 2175 365 814 1037
Fragments arranged by size:
2175 1037 814 451 365 228
ApaI G_GGCC'C
Cuts at: 0 737 5070
Size: 737 4333
ApoI r'AATT_y
Cuts at: 0 149 286 491 1177 1329 3192 3484 3625
Size: 149 137 205 686 152 1863 292 141
Cuts at: 3625 5070
Size: 1445
Fragments arranged by size:
1863 1445 686 292 205 152 149 141
137
AseI AT'TA_AT
Cuts at: 0 1376 5070
Size: 1376 3694
AvaI C'yCGr_G
Cuts at: 0 1043 1567 3002 3863 5070
Size: 1043 524 1435 861 1207
Fragments arranged by size:
1435 1207 1043 861 524
BamHI G'GATC_C
Cuts at: 0 3598 5070
Size: 3598 1472
BanI G'GyrC_C
Cuts at: 0 1817 2796 4275 5070
Size: 1817 979 1479 795
Fragments arranged by size:
1817 1479 979 795
BanII G_rGCy'C
Cuts at: 0 737 2434 2644 2843 3226 3536 4270 5070
Size: 737 1697 210 199 383 310 734 800
Fragments arranged by size:
1697 800 737 734 383 310 210 199
BbeI G_GCGC'C
Cuts at: 0 1821 5070
Size: 1821 3249
BbsI GAAGACnn'nnnn_
Cuts at: 0 670 798 2174 2497 4046 4436 5070
Size: 670 128 1376 323 1549 390 634
Fragments arranged by size:
1549 1376 670 634 390 323 128
BbvCI CC'TCA_GC
Cuts at: 0 2309 5070
Size: 2309 2761
BciVI GTATCCnnnnn_n'
Cuts at: 0 3702 5070
Size: 3702 1368
BfrBI ATG'CAT
Cuts at: 0 3423 5070
Size: 3423 1647
BglII A'GATC_T
Cuts at: 0 558 4356 5070
Size: 558 3798 714
Fragments arranged by size:
3798 714 558
BlpI GC'TnA_GC
Cuts at: 0 97 2393 3220 5070
Size: 97 2296 827 1850
Fragments arranged by size:
2296 1850 827 97
Bme1580I G_kGCm'C
Cuts at: 0 58 737 992 2122 2208 4280 5070
Size: 58 679 255 1130 86 2072 790
Fragments arranged by size:
2072 1130 790 679 255 86 58
BmgBI CAC'GTC
Cuts at: 0 658 5070
Size: 658 4412
BmrI ACTGGGnnnn_n'
Cuts at: 0 470 1497 3179 4881 5070
Size: 470 1027 1682 1702 189
Fragments arranged by size:
1702 1682 1027 470 189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4244 5070
Size: 4244 826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1023 1482 1561 2605 3340 3358 4280
Size: 701 322 459 79 1044 735 18 922
Cuts at: 4280 4324 4337 4916 5070
Size: 44 13 579 154
Fragments arranged by size:
1044 922 735 701 579 459 322 154
79 44 18 13
Bpu10I CC'TnA_GC
Cuts at: 0 2081 2309 2579 2876 4322 5070
Size: 2081 228 270 297 1446 748
Fragments arranged by size:
2081 1446 748 297 270 228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 3088 4214 5070
Size: 259 2829 1126 856
Fragments arranged by size:
2829 1126 856 259
BsaI GGTCTCn'nnnn_
Cuts at: 0 470 2368 3656 5056 5070
Size: 470 1898 1288 1400 14
Fragments arranged by size:
1898 1400 1288 470 14
BsaBI GATnn'nnATC
Cuts at: 0 4138 5070
Size: 4138 932
BsaHI Gr'CG_yC
Cuts at: 0 1785 1809 1818 3090 5070
Size: 1785 24 9 1272 1980
Fragments arranged by size:
1980 1785 1272 24 9
BsaWI w'CCGG_w
Cuts at: 0 1266 5070
Size: 1266 3804
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2825 2855 5070
Size: 2825 30 2215
Fragments arranged by size:
2825 2215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 1230 1558 3983 4165 4816 5070
Size: 94 1136 328 2425 182 651 254
Fragments arranged by size:
2425 1136 651 328 254 182 94
BseYI C'CCAG_C
Cuts at: 0 730 1583 5070
Size: 730 853 3487
Fragments arranged by size:
3487 853 730
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 3911 4211 4643 5070
Size: 562 3349 300 432 427
Fragments arranged by size:
3349 562 432 427 300
BsiEI CG_ry'CG
Cuts at: 0 111 157 5070
Size: 111 46 4913
Fragments arranged by size:
4913 111 46
BsiHKAI G_wGCw'C
Cuts at: 0 2580 2843 3536 4738 5070
Size: 2580 263 693 1202 332
Fragments arranged by size:
2580 1202 693 332 263
BsiWI C'GTAC_G
Cuts at: 0 3711 5070
Size: 3711 1359
BsmI GAATG_Cn'
Cuts at: 0 1879 3423 4824 5070
Size: 1879 1544 1401 246
Fragments arranged by size:
1879 1544 1401 246
Bsp1286I G_dGCh'C
Cuts at: 0 58 737 992 2122 2208 2434 2580 2644
Size: 58 679 255 1130 86 226 146 64
Cuts at: 2644 2843 3226 3536 4270 4280 4738 5070
Size: 199 383 310 734 10 458 332
Fragments arranged by size:
1130 734 679 458 383 332 310 255
226 199 146 86 64 58 10
BspHI T'CATG_A
Cuts at: 0 1616 1922 2568 2762 5070
Size: 1616 306 646 194 2308
Fragments arranged by size:
2308 1616 646 306 194
BsrBI CCG'CTC
Cuts at: 0 96 2019 2085 5070
Size: 96 1923 66 2985
Fragments arranged by size:
2985 1923 96 66
BsrDI GCAATG_nn'
Cuts at: 0 251 761 2828 5070
Size: 251 510 2067 2242
Fragments arranged by size:
2242 2067 510 251
BsrFI r'CCGG_y
Cuts at: 0 136 5070
Size: 136 4934
BsrGI T'GTAC_A
Cuts at: 0 1655 1959 5070
Size: 1655 304 3111
Fragments arranged by size:
3111 1655 304
BssSI C'ACGA_G
Cuts at: 0 1700 1837 5070
Size: 1700 137 3233
Fragments arranged by size:
3233 1700 137
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1647 2251 4198 5070
Size: 1647 604 1947 872
Fragments arranged by size:
1947 1647 872 604
BstBI TT'CG_AA
Cuts at: 0 2343 5070
Size: 2343 2727
BstEII G'GTnAC_C
Cuts at: 0 396 783 4158 5070
Size: 396 387 3375 912
Fragments arranged by size:
3375 912 396 387
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 1505 3378 5070
Size: 744 761 1873 1692
Fragments arranged by size:
1873 1692 761 744
BstYI r'GATC_y
Cuts at: 0 90 304 317 345 558 1866 2736 3309
Size: 90 214 13 28 213 1308 870 573
Cuts at: 3309 3598 4356 5052 5070
Size: 289 758 696 18
Fragments arranged by size:
1308 870 758 696 573 289 214 213
90 28 18 13
BstZ17I GTA'TAC
Cuts at: 0 1082 5070
Size: 1082 3988
Bsu36I CC'TnA_GG
Cuts at: 0 581 1813 5070
Size: 581 1232 3257
Fragments arranged by size:
3257 1232 581
BtgI C'CryG_G
Cuts at: 0 1405 1898 3371 5070
Size: 1405 493 1473 1699
Fragments arranged by size:
1699 1473 1405 493
BtsI GCAGTG_nn'
Cuts at: 0 2029 3115 3745 3984 5070
Size: 2029 1086 630 239 1086
Fragments arranged by size:
2029 1086 1086 630 239
DraIII CAC_nnn'GTG
Cuts at: 0 2910 5070
Size: 2910 2160
DrdI GACnn_nn'nnGTC
Cuts at: 0 2224 3678 5070
Size: 2224 1454 1392
Fragments arranged by size:
2224 1454 1392
EaeI y'GGCC_r
Cuts at: 0 108 154 3818 4652 5070
Size: 108 46 3664 834 418
Fragments arranged by size:
3664 834 418 108 46
EagI C'GGCC_G
Cuts at: 0 108 154 5070
Size: 108 46 4916
Fragments arranged by size:
4916 108 46
EarI CTCTTCn'nnn_
Cuts at: 0 640 1298 1437 2555 3575 4124 4258 4463
Size: 640 658 139 1118 1020 549 134 205
Cuts at: 4463 4506 4670 5070
Size: 43 164 400
Fragments arranged by size:
1118 1020 658 640 549 400 205 164
139 134 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 142 1296 3189 4413 5070
Size: 46 96 1154 1893 1224 657
Fragments arranged by size:
1893 1224 1154 657 96 46
EcoICRI GAG'CTC
Cuts at: 0 2841 3534 5070
Size: 2841 693 1536
Fragments arranged by size:
2841 1536 693
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 700 701 727 1023 1072 1482 1561
Size: 334 366 1 26 296 49 410 79
Cuts at: 1561 1763 1949 2605 2701 3340 3358 3682 3743
Size: 202 186 656 96 639 18 324 61
Cuts at: 3743 4280 4324 4337 4448 4487 4916 5070
Size: 537 44 13 111 39 429 154
Fragments arranged by size:
656 639 537 429 410 366 334 324
296 202 186 154 111 96 79 61
49 44 39 26 18 13 1
EcoO109I rG'GnC_Cy
Cuts at: 0 3732 4850 5070
Size: 3732 1118 220
Fragments arranged by size:
3732 1118 220
EcoRI G'AATT_C
Cuts at: 0 491 3192 5070
Size: 491 2701 1878
Fragments arranged by size:
2701 1878 491
EcoRV GAT'ATC
Cuts at: 0 442 1236 2152 5070
Size: 442 794 916 2918
Fragments arranged by size:
2918 916 794 442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4738 5070
Size: 4738 332
FspI TGC'GCA
Cuts at: 0 4830 5070
Size: 4830 240
HaeII r_GCGC'y
Cuts at: 0 228 1821 5070
Size: 228 1593 3249
Fragments arranged by size:
3249 1593 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1220 1225 1252 1257 1683 1715 2626 2658
Size: 1220 5 27 5 426 32 911 32
Cuts at: 2658 3061 3093 3790 3822 4759 4791 5070
Size: 403 32 697 32 937 32 279
Fragments arranged by size:
1220 937 911 697 426 403 279 32
32 32 32 32 27 5 5
HincII GTy'rAC
Cuts at: 0 864 5070
Size: 864 4206
HindIII A'AGCT_T
Cuts at: 0 198 5070
Size: 198 4872
HpaI GTT'AAC
Cuts at: 0 864 5070
Size: 864 4206
KasI G'GCGC_C
Cuts at: 0 1817 5070
Size: 1817 3253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1306 2131 2514 2690 4332 5070
Size: 1306 825 383 176 1642 738
Fragments arranged by size:
1642 1306 825 738 383 176
MscI TGG'CCA
Cuts at: 0 3820 4654 5070
Size: 3820 834 416
Fragments arranged by size:
3820 834 416
MslI CAynn'nnrTG
Cuts at: 0 628 832 1376 2573 3153 3376 5070
Size: 628 204 544 1197 580 223 1694
Fragments arranged by size:
1694 1197 628 580 544 223 204
MspA1I CmG'CkG
Cuts at: 0 2397 3560 3605 3910 5070
Size: 2397 1163 45 305 1160
Fragments arranged by size:
2397 1163 1160 305 45
NarI GG'CG_CC
Cuts at: 0 1818 5070
Size: 1818 3252
NcoI C'CATG_G
Cuts at: 0 1898 3371 5070
Size: 1898 1473 1699
Fragments arranged by size:
1898 1699 1473
NdeI CA'TA_TG
Cuts at: 0 504 1148 4056 4393 5070
Size: 504 644 2908 337 677
Fragments arranged by size:
2908 677 644 504 337
NsiI A_TGCA'T
Cuts at: 0 3425 5070
Size: 3425 1645
NspI r_CATG'y
Cuts at: 0 831 1344 2036 2048 2069 4170 4201 5070
Size: 831 513 692 12 21 2101 31 869
Fragments arranged by size:
2101 869 831 692 513 31 21 12
PciI A'CATG_T
Cuts at: 0 2044 2065 4166 5070
Size: 2044 21 2101 904
Fragments arranged by size:
2101 2044 904 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 167 2440 2811 3846 5070
Size: 167 2273 371 1035 1224
Fragments arranged by size:
2273 1224 1035 371 167
PfoI T'CCnGG_A
Cuts at: 0 341 1349 4361 5070
Size: 341 1008 3012 709
Fragments arranged by size:
3012 1008 709 341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 450 482 5070
Size: 450 32 4588
Fragments arranged by size:
4588 450 32
PspOMI G'GGCC_C
Cuts at: 0 733 5070
Size: 733 4337
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1055 1087 5070
Size: 1055 32 3983
Fragments arranged by size:
3983 1055 32
PstI C_TGCA'G
Cuts at: 0 1533 3740 4710 5070
Size: 1533 2207 970 360
Fragments arranged by size:
2207 1533 970 360
PvuII CAG'CTG
Cuts at: 0 2397 3560 3605 3910 5070
Size: 2397 1163 45 305 1160
Fragments arranged by size:
2397 1163 1160 305 45
RsrII CG'GwC_CG
Cuts at: 0 3230 5070
Size: 3230 1840
SacI G_AGCT'C
Cuts at: 0 2843 3536 5070
Size: 2843 693 1534
Fragments arranged by size:
2843 1534 693
SapI GCTCTTCn'nnn_
Cuts at: 0 4258 5070
Size: 4258 812
SfcI C'TryA_G
Cuts at: 0 1529 2833 2887 3523 3736 4706 5070
Size: 1529 1304 54 636 213 970 364
Fragments arranged by size:
1529 1304 970 636 364 213 54
SfoI GGC'GCC
Cuts at: 0 1819 5070
Size: 1819 3251
SmlI C'TyrA_G
Cuts at: 0 238 1043 3002 3067 3184 3863 4229 5070
Size: 238 805 1959 65 117 679 366 841
Fragments arranged by size:
1959 841 805 679 366 238 117 65
SpeI A'CTAG_T
Cuts at: 0 1164 5070
Size: 1164 3906
SphI G_CATG'C
Cuts at: 0 2036 4201 5070
Size: 2036 2165 869
Fragments arranged by size:
2165 2036 869
StuI AGG'CCT
Cuts at: 0 2482 5070
Size: 2482 2588
StyI C'CwwG_G
Cuts at: 0 161 737 1898 3371 4246 4571 5070
Size: 161 576 1161 1473 875 325 499
Fragments arranged by size:
1473 1161 875 576 499 325 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 526 1957 2913 3247 4433 4912 5070
Size: 526 1431 956 334 1186 479 158
Fragments arranged by size:
1431 1186 956 526 479 334 158
TatI w'GTAC_w
Cuts at: 0 1655 1759 1959 5070
Size: 1655 104 200 3111
Fragments arranged by size:
3111 1655 200 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1253 1285 5070
Size: 1253 32 3785
Fragments arranged by size:
3785 1253 32
Tth111I GACn'n_nGTC
Cuts at: 0 669 5070
Size: 669 4401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 1632 2463 3456 5070
Size: 258 1374 831 993 1614
Fragments arranged by size:
1614 1374 993 831 258
XhoI C'TCGA_G
Cuts at: 0 1043 3002 3863 5070
Size: 1043 1959 861 1207
Fragments arranged by size:
1959 1207 1043 861
XmnI GAAnn'nnTTC
Cuts at: 0 491 2003 2564 3029 4043 4133 5070
Size: 491 1512 561 465 1014 90 937
Fragments arranged by size:
1512 1014 937 561 491 465 90
ZraI GAC'GTC
Cuts at: 0 1786 5070
Size: 1786 3284
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AlfI AlwNI ApaI
ApoI AseI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI MmeI MscI MslI MspA1I NarI
NcoI NdeI NsiI NspI PciI PflMI PfoI PpiI
PspOMI PsrI PstI PvuII RsrII SacI SapI SfcI
SfoI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AleI AloI
ApaLI AscI AsiSI AvrII BaeI BcgI BclI BglI
BmtI BsaAI BsmBI BspEI BspMI BssHII BtgZI ClaI
CspCI DraI EcoNI FseI FspAI KpnI MfeI MluI
NaeI NgoMIV NheI NotI NruI PacI PasI PmeI
PmlI PpuMI PshAI PsiI PspXI PvuI SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SrfI SspI SwaI XbaI XmaI