(Circular) (Six-base) MAPSORT of: pFN21AA2004.seq Check: 4132 from: 1 to: 9472 pFN21AA2004 9472 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7282 278 Size: 53 83 186 936 5746 2468 Fragments arranged by size: 5746 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2545 5382 6815 1937 Size: 608 2837 1433 4594 Fragments arranged by size: 4594 2837 1433 608 Acc65I G'GTAC_C Cuts at: 6793 6793 Size: 9472 AclI AA'CG_TT Cuts at: 1563 7599 7972 1563 Size: 6036 373 3063 Fragments arranged by size: 6036 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2658 3317 7535 8919 9187 1632 Size: 1026 659 4218 1384 268 1917 Fragments arranged by size: 4218 1917 1384 1026 659 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9275 AflIII A'CryG_T Cuts at: 1203 2483 8371 9348 1203 Size: 1280 5888 977 1327 Fragments arranged by size: 5888 1327 1280 977 AgeI A'CCGG_T Cuts at: 8303 8303 Size: 9472 AhdI GACnn_n'nnGTC Cuts at: 8201 8201 Size: 9472 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2866 5272 6453 1184 Size: 1682 2406 1181 4203 Fragments arranged by size: 4203 2406 1682 1181 AlwNI CAG_nnn'CTG Cuts at: 7215 8787 7215 Size: 1572 7900 ApaLI G'TGCA_C Cuts at: 3737 7529 8685 3737 Size: 3792 1156 4524 Fragments arranged by size: 4524 3792 1156 ApoI r'AATT_y Cuts at: 1493 2106 2507 2591 2645 2686 3017 3140 3804 Size: 613 401 84 54 41 331 123 664 Cuts at: 3804 4016 4025 4274 4823 4868 5047 5081 5631 Size: 212 9 249 549 45 179 34 550 Cuts at: 5631 5786 6163 6388 6781 7007 7159 9268 1493 Size: 155 377 225 393 226 152 2109 1697 Fragments arranged by size: 2109 1697 664 613 550 549 401 393 377 331 249 226 225 212 179 155 152 123 84 54 45 41 34 9 AseI AT'TA_AT Cuts at: 160 1032 3785 6528 8026 160 Size: 872 2753 2743 1498 1606 Fragments arranged by size: 2753 2743 1606 1498 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9472 AvaI C'yCGr_G Cuts at: 1870 1943 6797 1870 Size: 73 4854 4545 Fragments arranged by size: 4854 4545 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2472 2505 2472 Size: 33 9439 BamHI G'GATC_C Cuts at: 4542 5725 6802 7169 4542 Size: 1183 1077 367 6845 Fragments arranged by size: 6845 1183 1077 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4886 6793 8248 618 Size: 325 217 627 3099 1907 1455 1842 Fragments arranged by size: 3099 1907 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2430 6045 6791 1391 Size: 1039 3615 746 4072 Fragments arranged by size: 4072 3615 1039 746 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2829 3603 4053 4881 6521 928 Size: 971 930 774 450 828 1640 3879 Fragments arranged by size: 3879 1640 971 930 828 774 450 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7663 7697 1903 Size: 34 5726 34 3678 Fragments arranged by size: 5726 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5237 5559 6206 7364 8574 5237 Size: 322 647 1158 1210 6135 Fragments arranged by size: 6135 1210 1158 647 322 BclI T'GATC_A Cuts at: 1548 4662 1548 Size: 3114 6358 BfrBI ATG'CAT Cuts at: 5249 6418 5249 Size: 1169 8303 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8083 136 Size: 107 122 71 631 605 6411 1525 Fragments arranged by size: 6411 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3766 9467 3766 Size: 5701 3771 BlpI GC'TnA_GC Cuts at: 6889 6889 Size: 9472 Bme1580I G_kGCm'C Cuts at: 1790 3741 4889 7533 8689 1790 Size: 1951 1148 2644 1156 2573 Fragments arranged by size: 2644 2573 1951 1156 1148 BmgBI CAC'GTC Cuts at: 1318 2125 1318 Size: 807 8665 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2271 5326 5381 Size: 416 53 83 399 24 854 3055 55 Cuts at: 5381 7203 8161 442 Size: 1822 958 1753 Fragments arranged by size: 3055 1822 1753 958 854 416 399 83 55 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9472 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3686 3686 Size: 9472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5621 6193 8132 1239 Size: 4382 572 1939 2579 Fragments arranged by size: 4382 2579 1939 572 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5467 6360 7597 8462 8760 5467 Size: 893 1237 865 298 6179 Fragments arranged by size: 6179 1237 893 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 8135 882 Size: 617 6636 2219 Fragments arranged by size: 6636 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2693 4378 7263 493 Size: 711 1489 1685 2885 2702 Fragments arranged by size: 2885 2702 1685 1489 711 BsaBI GATnn'nnATC Cuts at: 2061 9466 2061 Size: 7405 2067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7279 7661 8296 275 Size: 53 83 186 936 5746 382 635 1451 Fragments arranged by size: 5746 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4707 7905 8303 8577 8724 9135 4707 Size: 3198 398 274 147 411 5044 Fragments arranged by size: 5044 3198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4317 4347 1178 Size: 30 165 30 2914 30 6303 Fragments arranged by size: 6303 2914 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 9472 BseYI C'CCAG_C Cuts at: 3470 8675 9171 3470 Size: 5205 496 3771 Fragments arranged by size: 5205 3771 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4188 1163 Size: 573 2452 6447 Fragments arranged by size: 6447 2452 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6922 7683 8711 1540 Size: 275 186 4921 761 1028 2301 Fragments arranged by size: 4921 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2430 3741 7533 7618 8689 2430 Size: 1311 3792 85 1071 3213 Fragments arranged by size: 3792 3213 1311 1071 85 BsmI GAATG_Cn' Cuts at: 2853 2954 5249 5489 6983 7076 2853 Size: 101 2295 240 1494 93 5249 Fragments arranged by size: 5249 2295 1494 240 101 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3431 3431 Size: 9472 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2430 3741 4889 6045 6791 7533 7618 Size: 399 640 1311 1148 1156 746 742 85 Cuts at: 7618 8689 1391 Size: 1071 2174 Fragments arranged by size: 2174 1311 1156 1148 1071 746 742 640 399 85 BspEI T'CCGG_A Cuts at: 4707 9135 4707 Size: 4428 5044 BspHI T'CATG_A Cuts at: 3948 4665 7361 9091 3948 Size: 717 2696 1730 4329 Fragments arranged by size: 4329 2696 1730 717 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3483 6827 844 Size: 2639 3344 3489 Fragments arranged by size: 3489 3344 2639 BsrBI CCG'CTC Cuts at: 1394 1823 7359 1394 Size: 429 5536 3507 Fragments arranged by size: 5536 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2391 2659 3493 4158 4365 5311 7967 Size: 1183 1143 268 834 665 207 946 2656 Cuts at: 7967 8141 65 Size: 174 1396 Fragments arranged by size: 2656 1396 1183 1143 946 834 665 268 207 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8116 8303 1700 Size: 110 6306 187 2869 Fragments arranged by size: 6306 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9472 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9472 BssSI C'ACGA_G Cuts at: 7532 8544 7532 Size: 1012 8460 BstAPI GCAn_nnn'nTGC Cuts at: 794 5095 5477 794 Size: 4301 382 4789 Fragments arranged by size: 4789 4301 382 BstBI TT'CG_AA Cuts at: 8276 8276 Size: 9472 BstEII G'GTnAC_C Cuts at: 1184 4333 1184 Size: 3149 6323 BstXI CCAn_nnnn'nTGG Cuts at: 3540 3540 Size: 9472 BstYI r'GATC_y Cuts at: 1972 3664 3700 3766 4542 5725 6802 7169 7554 Size: 1692 36 66 776 1183 1077 367 385 Cuts at: 7554 7571 9023 9109 9121 9467 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1692 1452 1183 1077 776 385 367 346 86 66 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2734 513 Size: 552 939 730 7251 Fragments arranged by size: 7251 939 730 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5139 487 Size: 683 56 3913 4820 Fragments arranged by size: 4820 3913 683 56 BtsI GCAGTG_nn' Cuts at: 6988 7782 7802 6988 Size: 794 20 8658 Fragments arranged by size: 8658 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2853 2888 529 Size: 35 2289 35 7113 Fragments arranged by size: 7113 2289 35 35 DraI TTT'AAA Cuts at: 3091 3103 6021 6776 3091 Size: 12 2918 755 5787 Fragments arranged by size: 5787 2918 755 12 DraIII CAC_nnn'GTG Cuts at: 4817 4817 Size: 9472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2831 8479 809 Size: 507 1515 5648 1802 Fragments arranged by size: 5648 1802 1515 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6174 6323 6919 Size: 54 1425 50 275 18 4344 149 596 Cuts at: 6919 7808 9437 8 Size: 889 1629 43 Fragments arranged by size: 4344 1629 1425 889 596 275 149 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6919 1537 Size: 275 5107 4090 Fragments arranged by size: 5107 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2622 3834 4099 4776 5862 7402 1353 Size: 1269 1212 265 677 1086 1540 3423 Fragments arranged by size: 3423 1540 1269 1212 1086 677 265 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8059 8433 8579 387 Size: 1064 138 6470 374 146 1280 Fragments arranged by size: 6470 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2428 2428 Size: 9472 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2658 3317 5621 6193 7535 8132 8919 Size: 393 1026 659 2304 572 1342 597 787 Cuts at: 8919 9187 1239 Size: 268 1524 Fragments arranged by size: 2304 1524 1342 1026 787 659 597 572 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2556 2556 Size: 9472 EcoRI G'AATT_C Cuts at: 2507 2591 2686 4025 6781 7159 2507 Size: 84 95 1339 2756 378 4820 Fragments arranged by size: 4820 2756 1339 378 95 84 EcoRV GAT'ATC Cuts at: 3946 3946 Size: 9472 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2180 3788 4125 5314 5633 6263 2180 Size: 1608 337 1189 319 630 5389 Fragments arranged by size: 5389 1608 1189 630 337 319 FspI TGC'GCA Cuts at: 7978 7978 Size: 9472 HaeII r_GCGC'y Cuts at: 1678 8619 1678 Size: 6941 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3190 3222 3323 3355 3433 Size: 32 135 32 1618 32 101 32 78 Cuts at: 3433 3465 4935 4967 5673 5705 6698 6730 8113 Size: 32 1470 32 706 32 993 32 1383 Cuts at: 8113 8145 8187 8219 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1618 1470 1383 993 706 135 101 78 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6683 6816 7062 669 Size: 988 281 4745 133 246 3079 Fragments arranged by size: 4745 3079 988 281 246 133 HindIII A'AGCT_T Cuts at: 748 3363 748 Size: 2615 6857 HpaI GTT'AAC Cuts at: 7062 7062 Size: 9472 KpnI G_GTAC'C Cuts at: 6797 6797 Size: 9472 MfeI C'AATT_G Cuts at: 6435 6613 7071 6435 Size: 178 458 8836 Fragments arranged by size: 8836 458 178 MluI A'CGCG_T Cuts at: 9348 9348 Size: 9472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2514 2860 5010 6223 8586 8770 1305 Size: 1209 346 2150 1213 2363 184 2007 Fragments arranged by size: 2363 2150 2007 1213 1209 346 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6176 6325 10 Size: 54 1425 343 4344 149 3157 Fragments arranged by size: 4344 3157 1425 343 149 54 MslI CAynn'nnrTG Cuts at: 518 1166 3798 5124 5136 5184 7430 7789 7948 Size: 648 2632 1326 12 48 2246 359 159 Cuts at: 7948 518 Size: 2042 Fragments arranged by size: 2632 2246 2042 1326 648 359 159 48 12 MspA1I CmG'CkG Cuts at: 1606 3474 6888 7565 8713 8958 1606 Size: 1868 3414 677 1148 245 2120 Fragments arranged by size: 3414 2120 1868 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2734 513 Size: 552 939 730 7251 Fragments arranged by size: 7251 939 730 552 NdeI CA'TA_TG Cuts at: 387 4783 6414 387 Size: 4396 1631 3445 Fragments arranged by size: 4396 3445 1631 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9472 NotI GC'GGCC_GC Cuts at: 6919 6919 Size: 9472 NsiI A_TGCA'T Cuts at: 5251 6420 5251 Size: 1169 8303 NspI r_CATG'y Cuts at: 1132 1229 2487 5480 6830 8375 1132 Size: 97 1258 2993 1350 1545 2229 Fragments arranged by size: 2993 2229 1545 1350 1258 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9472 PciI A'CATG_T Cuts at: 2483 8371 2483 Size: 5888 3584 PflMI CCAn_nnn'nTGG Cuts at: 1108 3908 4226 5266 1108 Size: 2800 318 1040 5314 Fragments arranged by size: 5314 2800 1040 318 PmeI GTTT'AAAC Cuts at: 6776 6776 Size: 9472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5036 5068 7541 7573 9054 9086 5036 Size: 32 2473 32 1481 32 5422 Fragments arranged by size: 5422 2473 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9472 PsiI TTA'TAA Cuts at: 7042 7042 Size: 9472 PstI C_TGCA'G Cuts at: 830 4437 6824 830 Size: 3607 2387 3478 Fragments arranged by size: 3607 3478 2387 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9472 PvuII CAG'CTG Cuts at: 3474 3474 Size: 9472 SacI G_AGCT'C Cuts at: 2430 2430 Size: 9472 SalI G'TCGA_C Cuts at: 1936 6814 1936 Size: 4878 4594 SapI GCTCTTCn'nnn_ Cuts at: 4776 4776 Size: 9472 SbfI CC_TGCA'GG Cuts at: 6824 6824 Size: 9472 ScaI AGT'ACT Cuts at: 2896 4439 7720 9432 2896 Size: 1543 3281 1712 2936 Fragments arranged by size: 3281 2936 1712 1543 SfcI C'TryA_G Cuts at: 826 1045 2546 4433 4455 6820 7955 8636 8827 Size: 219 1501 1887 22 2365 1135 681 191 Cuts at: 8827 826 Size: 1471 Fragments arranged by size: 2365 1887 1501 1471 1135 681 219 191 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9472 SmaI CCC'GGG Cuts at: 1872 6799 1872 Size: 4927 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5482 6375 7576 8477 8739 820 Size: 197 926 3539 893 1201 901 262 1553 Fragments arranged by size: 3539 1553 1201 926 901 893 262 197 SnaBI TAC'GTA Cuts at: 493 4378 7263 493 Size: 3885 2885 2702 Fragments arranged by size: 3885 2885 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9472 SphI G_CATG'C Cuts at: 1132 6830 1132 Size: 5698 3774 SspI AAT'ATT Cuts at: 5 52 4732 5854 7396 5 Size: 47 4680 1122 1542 2081 Fragments arranged by size: 4680 2081 1542 1122 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9472 StyI C'CwwG_G Cuts at: 513 1065 2004 2734 2772 4239 4740 6911 513 Size: 552 939 730 38 1467 501 2171 3074 Fragments arranged by size: 3074 2171 1467 939 730 552 501 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3282 3756 7499 7516 7669 7854 8299 Size: 1255 1702 474 3743 17 153 185 445 Cuts at: 8299 325 Size: 1498 Fragments arranged by size: 3743 1702 1498 1255 474 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2390 2894 3240 Size: 274 80 33 51 1443 413 504 346 Cuts at: 3240 4384 4437 4467 7718 9430 96 Size: 1144 53 30 3251 1712 138 Fragments arranged by size: 3251 1712 1443 1144 504 413 346 274 138 80 53 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4924 4956 9432 9464 4924 Size: 32 4476 32 4932 Fragments arranged by size: 4932 4476 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9472 XbaI T'CTAG_A Cuts at: 6808 6808 Size: 9472 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9472 XmaI C'CCGG_G Cuts at: 1870 6797 1870 Size: 4927 4545 XmnI GAAnn'nnTTC Cuts at: 4274 4733 4850 5051 7601 4274 Size: 459 117 201 2550 6145 Fragments arranged by size: 6145 2550 459 201 117 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7280 276 Size: 53 83 186 936 5746 2468 Fragments arranged by size: 5746 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI