Restriction map for
pFN21AA1964
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AvrII BbsI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsiWI BsmBI
Bsp1407I BspDI BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI DraI
DraIII DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KspI MamI MfeI
MluI MseI MunI NaeI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 2282 GGTACC G^GTACC
AccIII 2178 TCCGGA T^CCGGA
ApaI 1087 GGGCCC GGGCC^C
Asp718I 2282 GGTACC G^GTACC
AspI 1225 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2063 TGCGCA TGC^GCA
BamHI 557 GGATCC G^GATCC
BglI 859 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 2178 TCCGGA T^CCGGA
BsmI 2025 GAATGC GAATGC(1/-1)
BspEI 2178 TCCGGA T^CCGGA
BspHI 947 TCATGA T^CATGA
BspLU11I 659 ACATGT A^CATGT
CpoI 347 CGGTCCG CG^G[AT]CCG
FspI 2063 TGCGCA TGC^GCA
HincII 640 GTCAAC GT[CT]^[GA]AC
HindII 640 GTCAAC GT[CT]^[GA]AC
KpnI 2282 GGTACC GGTAC^C
MroI 2178 TCCGGA T^CCGGA
NotI 8 GCGGCCGC GC^GGCCGC
PflFI 1225 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 947 TCATGA T^CATGA
RsrII 347 CGGTCCG CG^G[AT]CCG
SacI 898 GAGCTC GAGCT^C
SapI 2337 GCTCTTC GCTCTTC(1/4)
ScaI 785 AGTACT AGT^ACT
SmaI 136 CCCGGG CCC^GGG
StuI 1103 AGGCCT AGG^CCT
TfiI 1212 GATTC G^A[AT]TC
Tth111I 1225 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 711 TCTAGA T^CTAGA
XcmI 2231 CCAATGACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 136 CCCGGG C^CCGGG
XmaI 136 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Aor51HI 482 AGCGCT AGC^GCT
2360 AGCGCT
AspHI 898 GAGCTC G[AT]GC[AT]^C
2119 GTGCTC
BalI 1191 TGGCCA TGG^CCA
1559 TGGCCA
BbrPI 231 CACGTG CAC^GTG
996 CACGTG
BbvCI 1671 CCTCAGC CCTCAGC(-5/-2)
469 GCTGAGG
BglII 773 AGATCT A^GATCT
1568 AGATCT
BsaWI 2178 TCCGGA [AT]^CCGG[AT]
2195 TCCGGT
BsiHKAI 898 GAGCTC G[AT]GC[AT]^C
2119 GTGCTC
BsmAI 907 GAGAC GTCTC(1/5)
2107 GAGAC
BspMI 1917 ACCTGC ACCTGC(4/8)
2297 ACCTGC
BsrFI 84 GCCGGT [GA]^CCGG[CT]
254 ACCGGC
BstAPI 2089 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2151 GCAGACCCTGC
Cfr10I 84 GCCGGT [GA]^CCGG[CT]
254 ACCGGC
EagI 9 CGGCCG C^GGCCG
757 CGGCCG
EclXI 9 CGGCCG C^GGCCG
757 CGGCCG
Eco47III 482 AGCGCT AGC^GCT
2360 AGCGCT
Eco52I 9 CGGCCG C^GGCCG
757 CGGCCG
MluNI 1191 TGGCCA TGG^CCA
1559 TGGCCA
MscI 1191 TGGCCA TGG^CCA
1559 TGGCCA
NcoI 1322 CCATGG C^CATGG
2076 CCATGG
NspI 659 ACATGT [GA]CATG^[CT]
1355 ACATGC
PmaCI 231 CACGTG CAC^GTG
996 CACGTG
PmlI 231 CACGTG CAC^GTG
996 CACGTG
PpuMI 1427 GGGTCCT [GA]G^G[AT]CC[CT]
1965 AGGACCT
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# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsgI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII