Restriction map for
pFN21AA1964
(Linear) (Six-base) MAPSORT of: KIAA1964.seq Check: 9806 from: 1 to: 2367
KIAA1964.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2306 2367
Size: 2306 61
Acc65I G'GTAC_C
Cuts at: 0 2282 2367
Size: 2282 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 772 1438 2367
Size: 172 600 666 929
Fragments arranged by size:
929 666 600 172
AfeI AGC'GCT
Cuts at: 0 484 2362 2367
Size: 484 1878 5
Fragments arranged by size:
1878 484 5
AflIII A'CryG_T
Cuts at: 0 659 995 997 2367
Size: 659 336 2 1370
Fragments arranged by size:
1370 659 336 2
AleI CACnn'nnGTG
Cuts at: 0 998 1000 2367
Size: 998 2 1367
Fragments arranged by size:
1367 998 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1334 2011 2367
Size: 1334 677 356
Fragments arranged by size:
1334 677 356
AlwNI CAG_nnn'CTG
Cuts at: 0 1374 1998 2013 2157 2367
Size: 1374 624 15 144 210
Fragments arranged by size:
1374 624 210 144 15
ApaI G_GGCC'C
Cuts at: 0 1091 2367
Size: 1091 1276
AvaI C'yCGr_G
Cuts at: 0 37 136 789 1390 1812 1954 2367
Size: 37 99 653 601 422 142 413
Fragments arranged by size:
653 601 422 413 142 99 37
BamHI G'GATC_C
Cuts at: 0 557 2367
Size: 557 1810
BanI G'GyrC_C
Cuts at: 0 81 124 391 459 856 2082 2282 2367
Size: 81 43 267 68 397 1226 200 85
Fragments arranged by size:
1226 397 267 200 85 81 68 43
BanII G_rGCy'C
Cuts at: 0 47 205 902 1091 1178 1482 2178 2367
Size: 47 158 697 189 87 304 696 189
Fragments arranged by size:
697 696 304 189 189 158 87 47
BbeI G_GCGC'C
Cuts at: 0 85 463 860 2367
Size: 85 378 397 1507
Fragments arranged by size:
1507 397 378 85
BbvCI CC'TCA_GC
Cuts at: 0 470 1672 2367
Size: 470 1202 695
Fragments arranged by size:
1202 695 470
BglI GCCn_nnn'nGGC
Cuts at: 0 865 2367
Size: 865 1502
BglII A'GATC_T
Cuts at: 0 773 1568 2367
Size: 773 795 799
Fragments arranged by size:
799 795 773
Bme1580I G_kGCm'C
Cuts at: 0 816 1091 2087 2100 2367
Size: 816 275 996 13 267
Fragments arranged by size:
996 816 275 267 13
BmgBI CAC'GTC
Cuts at: 0 183 1227 2367
Size: 183 1044 1140
Fragments arranged by size:
1140 1044 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 787 847 856 1309 1327 2367
Size: 36 751 60 9 453 18 1040
Fragments arranged by size:
1040 751 453 60 36 18 9
Bpu10I CC'TnA_GC
Cuts at: 0 274 470 818 1672 2367
Size: 274 196 348 854 695
Fragments arranged by size:
854 695 348 274 196
BsaAI yAC'GTr
Cuts at: 0 233 998 2118 2367
Size: 233 765 1120 249
Fragments arranged by size:
1120 765 249 233
BsaHI Gr'CG_yC
Cuts at: 0 82 460 524 857 1846 2219 2367
Size: 82 378 64 333 989 373 148
Fragments arranged by size:
989 378 373 333 148 82 64
BsaWI w'CCGG_w
Cuts at: 0 2178 2195 2367
Size: 2178 17 172
Fragments arranged by size:
2178 172 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 235 265 2209 2239 2367
Size: 235 30 1944 30 128
Fragments arranged by size:
1944 235 128 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 664 745 784 865 1408 1510 1513
Size: 55 609 81 39 81 543 102 3
Cuts at: 1513 1516 1519 1531 1543 2367
Size: 3 3 12 12 824
Fragments arranged by size:
824 609 543 102 81 81 55 39
12 12 3 3 3
BseYI C'CCAG_C
Cuts at: 0 485 875 1090 1154 1761 2144 2367
Size: 485 390 215 64 607 383 223
Fragments arranged by size:
607 485 390 383 223 215 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 556 1323 1612 1988 2367
Size: 352 204 767 289 376 379
Fragments arranged by size:
767 379 376 352 289 204
BsiEI CG_ry'CG
Cuts at: 0 12 89 708 760 2367
Size: 12 77 619 52 1607
Fragments arranged by size:
1607 619 77 52 12
BsiHKAI G_wGCw'C
Cuts at: 0 902 2123 2367
Size: 902 1221 244
Fragments arranged by size:
1221 902 244
BsmI GAATG_Cn'
Cuts at: 0 2031 2367
Size: 2031 336
Bsp1286I G_dGCh'C
Cuts at: 0 47 205 816 902 1091 1178 1482 2087
Size: 47 158 611 86 189 87 304 605
Cuts at: 2087 2100 2123 2178 2367
Size: 13 23 55 189
Fragments arranged by size:
611 605 304 189 189 158 87 86
55 47 23 13
BspEI T'CCGG_A
Cuts at: 0 2178 2367
Size: 2178 189
BspHI T'CATG_A
Cuts at: 0 947 2367
Size: 947 1420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1926 2306 2367
Size: 1926 380 61
Fragments arranged by size:
1926 380 61
BsrBI CCG'CTC
Cuts at: 0 224 246 543 2367
Size: 224 22 297 1824
Fragments arranged by size:
1824 297 224 22
BsrFI r'CCGG_y
Cuts at: 0 84 254 2367
Size: 84 170 2113
Fragments arranged by size:
2113 170 84
BssHII G'CGCG_C
Cuts at: 0 186 406 512 514 2367
Size: 186 220 106 2 1853
Fragments arranged by size:
1853 220 186 106 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2095 2157 2367
Size: 2095 62 210
Fragments arranged by size:
2095 210 62
BstYI r'GATC_y
Cuts at: 0 218 557 773 1568 2072 2367
Size: 218 339 216 795 504 295
Fragments arranged by size:
795 504 339 295 218 216
BtgI C'CryG_G
Cuts at: 0 464 1322 2076 2367
Size: 464 858 754 291
Fragments arranged by size:
858 754 464 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 296 405 2367
Size: 296 109 1962
Fragments arranged by size:
1962 296 109
BtsI GCAGTG_nn'
Cuts at: 0 1296 1903 2254 2367
Size: 1296 607 351 113
Fragments arranged by size:
1296 607 351 113
EaeI y'GGCC_r
Cuts at: 0 9 53 757 1191 1473 1559 2367
Size: 9 44 704 434 282 86 808
Fragments arranged by size:
808 704 434 282 86 44 9
EagI C'GGCC_G
Cuts at: 0 9 757 2367
Size: 9 748 1610
Fragments arranged by size:
1610 748 9
EarI CTCTTCn'nnn_
Cuts at: 0 682 1050 1222 1520 2344 2367
Size: 682 368 172 298 824 23
Fragments arranged by size:
824 682 368 298 172 23
EcoICRI GAG'CTC
Cuts at: 0 900 2367
Size: 900 1467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 172 772 787 847 856 1309 1327
Size: 36 136 600 15 60 9 453 18
Cuts at: 1327 1438 2367
Size: 111 929
Fragments arranged by size:
929 600 453 136 111 60 36 18
15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 169 679 1110 1435 2367
Size: 169 510 431 325 932
Fragments arranged by size:
932 510 431 325 169
EcoO109I rG'GnC_Cy
Cuts at: 0 372 1087 1115 1428 1966 2315 2367
Size: 372 715 28 313 538 349 52
Fragments arranged by size:
715 538 372 349 313 52 28
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 624 1233 2367
Size: 624 609 1134
Fragments arranged by size:
1134 624 609
FspI TGC'GCA
Cuts at: 0 2065 2367
Size: 2065 302
FspAI rTGC'GCAy
Cuts at: 0 2065 2367
Size: 2065 302
HaeII r_GCGC'y
Cuts at: 0 85 151 384 463 486 860 1375 1881
Size: 85 66 233 79 23 374 515 506
Cuts at: 1881 2364 2367
Size: 483 3
Fragments arranged by size:
515 506 483 374 233 85 79 66
23 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 765 797 1387 1419 2209 2241 2302 2334
Size: 765 32 590 32 790 32 61 32
Cuts at: 2334 2367
Size: 33
Fragments arranged by size:
790 765 590 61 33 32 32 32
32
HincII GTy'rAC
Cuts at: 0 642 2367
Size: 642 1725
KasI G'GCGC_C
Cuts at: 0 81 459 856 2367
Size: 81 378 397 1511
Fragments arranged by size:
1511 397 378 81
KpnI G_GTAC'C
Cuts at: 0 2286 2367
Size: 2286 81
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 722 1466 2367
Size: 611 111 744 901
Fragments arranged by size:
901 744 611 111
MscI TGG'CCA
Cuts at: 0 1193 1561 2367
Size: 1193 368 806
Fragments arranged by size:
1193 806 368
MslI CAynn'nnrTG
Cuts at: 0 998 1000 1360 1993 2367
Size: 998 2 360 633 374
Fragments arranged by size:
998 633 374 360 2
MspA1I CmG'CkG
Cuts at: 0 14 291 1136 1416 1456 1743 1761 1824
Size: 14 277 845 280 40 287 18 63
Cuts at: 1824 2013 2144 2169 2367
Size: 189 131 25 198
Fragments arranged by size:
845 287 280 277 198 189 131 63
40 25 18 14
NarI GG'CG_CC
Cuts at: 0 82 460 857 2367
Size: 82 378 397 1510
Fragments arranged by size:
1510 397 378 82
NcoI C'CATG_G
Cuts at: 0 1322 2076 2367
Size: 1322 754 291
Fragments arranged by size:
1322 754 291
NotI GC'GGCC_GC
Cuts at: 0 9 2367
Size: 9 2358
NspI r_CATG'y
Cuts at: 0 663 1359 2367
Size: 663 696 1008
Fragments arranged by size:
1008 696 663
PasI CC'CwG_GG
Cuts at: 0 1125 2367
Size: 1125 1242
PciI A'CATG_T
Cuts at: 0 659 2367
Size: 659 1708
PfoI T'CCnGG_A
Cuts at: 0 833 1007 1220 2367
Size: 833 174 213 1147
Fragments arranged by size:
1147 833 213 174
PmlI CAC'GTG
Cuts at: 0 233 998 2367
Size: 233 765 1369
Fragments arranged by size:
1369 765 233
PpuMI rG'GwC_Cy
Cuts at: 0 1428 1966 2367
Size: 1428 538 401
Fragments arranged by size:
1428 538 401
PspOMI G'GGCC_C
Cuts at: 0 1087 2367
Size: 1087 1280
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 646 678 2367
Size: 646 32 1689
Fragments arranged by size:
1689 646 32
PstI C_TGCA'G
Cuts at: 0 263 1545 2162 2367
Size: 263 1282 617 205
Fragments arranged by size:
1282 617 263 205
PvuII CAG'CTG
Cuts at: 0 1416 1743 1824 2013 2169 2367
Size: 1416 327 81 189 156 198
Fragments arranged by size:
1416 327 198 189 156 81
RsrII CG'GwC_CG
Cuts at: 0 348 2367
Size: 348 2019
SacI G_AGCT'C
Cuts at: 0 902 2367
Size: 902 1465
SapI GCTCTTCn'nnn_
Cuts at: 0 2344 2367
Size: 2344 23
ScaI AGT'ACT
Cuts at: 0 787 2367
Size: 787 1580
SfcI C'TryA_G
Cuts at: 0 259 1541 1788 2158 2367
Size: 259 1282 247 370 209
Fragments arranged by size:
1282 370 259 247 209
SfoI GGC'GCC
Cuts at: 0 83 461 858 2367
Size: 83 378 397 1509
Fragments arranged by size:
1509 397 378 83
SmaI CCC'GGG
Cuts at: 0 138 2367
Size: 138 2229
SrfI GCCC'GGGC
Cuts at: 0 138 2367
Size: 138 2229
StuI AGG'CCT
Cuts at: 0 1105 2367
Size: 1105 1262
StyI C'CwwG_G
Cuts at: 0 980 1322 2076 2306 2367
Size: 980 342 754 230 61
Fragments arranged by size:
980 754 342 230 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 477 1006 2367
Size: 477 529 1361
Fragments arranged by size:
1361 529 477
TatI w'GTAC_w
Cuts at: 0 785 1598 2367
Size: 785 813 769
Fragments arranged by size:
813 785 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2287 2319 2367
Size: 2287 32 48
Fragments arranged by size:
2287 48 32
Tth111I GACn'n_nGTC
Cuts at: 0 1228 2367
Size: 1228 1139
XbaI T'CTAG_A
Cuts at: 0 711 2367
Size: 711 1656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2238 2367
Size: 2238 129
XmaI C'CCGG_G
Cuts at: 0 136 2367
Size: 136 2231
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI
ApaI AvaI BamHI BanI BanII BbeI BbvCI BglI
BglII Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BssHII BstAPI BstYI
BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NarI NcoI
NotI NspI PasI PciI PfoI PmlI PpuMI PspOMI
PsrI PstI PvuII RsrII SacI SapI ScaI SfcI
SfoI SmaI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BcgI
BciVI BclI BfrBI BlpI BmrI BmtI BplI BpuEI
BsaI BsaBI BsiWI BsmBI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EciI EcoRI EcoRV FseI HindIII HpaI MfeI
MluI NaeI NdeI NgoMIV NheI NruI NsiI PacI
PflMI PmeI PpiI PshAI PsiI PspXI PvuI SacII
SalI SanDI SbfI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI SwaI XhoI XmnI ZraI