(Circular) (Six-base) MAPSORT of: pFN21AA1773.seq Check: 7573 from: 1 to: 14599 pFN21AA1773 14599 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2821 3421 3790 5539 5923 6262 8569 9133 11263 Size: 600 369 1749 384 339 2307 564 2130 Cuts at: 11263 11426 2821 Size: 163 5994 Fragments arranged by size: 5994 2307 2130 1749 600 564 384 369 339 163 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5422 6930 12409 278 Size: 53 83 186 936 3886 1508 5479 2468 Fragments arranged by size: 5479 3886 2468 1508 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 8670 11942 1937 Size: 6733 3272 4594 Fragments arranged by size: 6733 4594 3272 Acc65I G'GTAC_C Cuts at: 2458 5680 11920 2458 Size: 3222 6240 5137 Fragments arranged by size: 6240 5137 3222 AclI AA'CG_TT Cuts at: 1563 12726 13099 1563 Size: 11163 373 3063 Fragments arranged by size: 11163 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4716 5234 5469 5631 7915 7989 8953 9253 Size: 3084 518 235 162 2284 74 964 300 Cuts at: 9253 9501 12662 14046 14314 1632 Size: 248 3161 1384 268 1917 Fragments arranged by size: 3161 3084 2284 1917 1384 964 518 300 268 248 235 162 74 AfeI AGC'GCT Cuts at: 6366 6493 6804 6366 Size: 127 311 14161 Fragments arranged by size: 14161 311 127 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 14402 AflIII A'CryG_T Cuts at: 1203 6655 6739 13498 14475 1203 Size: 5452 84 6759 977 1327 Fragments arranged by size: 6759 5452 1327 977 84 AgeI A'CCGG_T Cuts at: 13430 13430 Size: 14599 AhdI GACnn_n'nnGTC Cuts at: 13328 13328 Size: 14599 AleI CACnn'nnGTG Cuts at: 1166 4112 4427 6110 7832 8039 8159 9305 9465 Size: 2946 315 1683 1722 207 120 1146 160 Cuts at: 9465 10145 10175 11624 1166 Size: 680 30 1449 4141 Fragments arranged by size: 4141 2946 1722 1683 1449 1146 680 315 207 160 120 30 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4130 4631 7022 9676 1184 Size: 2946 501 2391 2654 6107 Fragments arranged by size: 6107 2946 2654 2391 501 AlwNI CAG_nnn'CTG Cuts at: 2519 2618 2722 5062 5231 5542 5591 5654 5926 Size: 99 104 2340 169 311 49 63 272 Cuts at: 5926 6005 6029 6907 7814 7838 9052 9530 9722 Size: 79 24 878 907 24 1214 478 192 Cuts at: 9722 9770 9839 10259 10391 10754 10919 10969 11048 Size: 48 69 420 132 363 165 50 79 Cuts at: 11048 11117 11348 11861 12342 13914 2519 Size: 69 231 513 481 1572 3204 Fragments arranged by size: 3204 2340 1572 1214 907 878 513 481 478 420 363 311 272 231 192 169 165 132 104 99 79 79 69 69 63 50 49 48 24 24 ApaI G_GGCC'C Cuts at: 2716 3954 4878 5096 6690 7129 7821 8571 8958 Size: 1238 924 218 1594 439 692 750 387 Cuts at: 8958 9012 10894 11340 2716 Size: 54 1882 446 5975 Fragments arranged by size: 5975 1882 1594 1238 924 750 692 446 439 387 218 54 ApaLI G'TGCA_C Cuts at: 3919 6093 9407 10056 12656 13812 3919 Size: 2174 3314 649 2600 1156 4706 Fragments arranged by size: 4706 3314 2600 2174 1156 649 ApoI r'AATT_y Cuts at: 1493 11908 12134 12286 14395 1493 Size: 10415 226 152 2109 1697 Fragments arranged by size: 10415 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 13153 160 Size: 872 12121 1606 Fragments arranged by size: 12121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 14599 AvaI C'yCGr_G Cuts at: 1870 1943 2442 3495 4326 4503 4638 4914 6128 Size: 73 499 1053 831 177 135 276 1214 Cuts at: 6128 6711 7485 8097 8211 9442 9648 9813 11682 Size: 583 774 612 114 1231 206 165 1869 Cuts at: 11682 11924 1870 Size: 242 4545 Fragments arranged by size: 4545 1869 1231 1214 1053 831 774 612 583 499 276 242 206 177 165 135 114 73 AvrII C'CTAG_G Cuts at: 10876 10876 Size: 14599 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2598 2631 2598 Size: 33 14566 BamHI G'GATC_C Cuts at: 3499 4148 4498 5326 5731 7093 8962 11929 12296 Size: 649 350 828 405 1362 1869 2967 367 Cuts at: 12296 3499 Size: 5802 Fragments arranged by size: 5802 2967 1869 1362 828 649 405 367 350 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2113 2120 2375 2458 2480 Size: 325 217 627 326 7 255 83 22 Cuts at: 2480 3512 3548 3773 4414 4421 4468 5039 5680 Size: 1032 36 225 641 7 47 571 641 Cuts at: 5680 5995 6119 6134 6940 7282 7427 7594 7688 Size: 315 124 15 806 342 145 167 94 Cuts at: 7688 7985 8369 9221 9974 10072 10447 10478 10532 Size: 297 384 852 753 98 375 31 54 Cuts at: 10532 10630 10792 11511 11920 13375 618 Size: 98 162 719 409 1455 1842 Fragments arranged by size: 1842 1455 1032 852 806 753 719 641 641 627 571 409 384 375 342 326 325 315 297 255 225 217 167 162 145 124 98 98 94 83 54 47 36 31 22 15 7 7 BanII G_rGCy'C Cuts at: 1391 2054 2716 3022 3038 3447 3732 3954 4759 Size: 663 662 306 16 409 285 222 805 Cuts at: 4759 4878 4928 5096 5570 6062 6081 6357 6690 Size: 119 50 168 474 492 19 276 333 Cuts at: 6690 7129 7187 7722 7821 8571 8768 8783 8853 Size: 439 58 535 99 750 197 15 70 Cuts at: 8853 8882 8958 9012 9382 9951 9971 10606 10894 Size: 29 76 54 370 569 20 635 288 Cuts at: 10894 11340 11384 11475 11918 1391 Size: 446 44 91 443 4072 Fragments arranged by size: 4072 805 750 663 662 635 569 535 492 474 446 443 439 409 370 333 306 288 285 276 222 197 168 119 99 91 76 70 58 54 50 44 29 20 19 16 15 BbeI G_GCGC'C Cuts at: 3552 6944 7692 3552 Size: 3392 748 10459 Fragments arranged by size: 10459 3392 748 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4704 5457 6598 6631 9489 9586 11533 Size: 971 2805 753 1141 33 2858 97 1947 Cuts at: 11533 928 Size: 3994 Fragments arranged by size: 3994 2858 2805 1947 1141 971 753 97 33 BbvCI CC'TCA_GC Cuts at: 3366 3630 4118 4290 5944 6030 6231 7472 9618 Size: 264 488 172 1654 86 201 1241 2146 Cuts at: 9618 10697 10908 11743 11836 11842 3366 Size: 1079 211 835 93 6 6123 Fragments arranged by size: 6123 2146 1654 1241 1079 835 488 264 211 201 172 93 86 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2180 2214 4532 4566 10187 Size: 34 50 34 159 34 2318 34 5621 Cuts at: 10187 10221 11237 11271 12790 12824 1903 Size: 34 1016 34 1519 34 3678 Fragments arranged by size: 5621 3678 2318 1519 1016 159 50 34 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 12491 13701 12491 Size: 1210 13389 BclI T'GATC_A Cuts at: 1548 8163 10105 11142 1548 Size: 6615 1942 1037 5005 Fragments arranged by size: 6615 5005 1942 1037 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3834 4256 4873 Size: 107 122 71 631 605 2162 422 617 Cuts at: 4873 5139 6685 9906 11171 11456 11730 13210 136 Size: 266 1546 3221 1265 285 274 1480 1525 Fragments arranged by size: 3221 2162 1546 1525 1480 1265 631 617 605 422 285 274 266 122 107 71 BglII A'GATC_T Cuts at: 5971 8718 14594 5971 Size: 2747 5876 5976 Fragments arranged by size: 5976 5876 2747 BlpI GC'TnA_GC Cuts at: 6488 6843 8190 8386 8782 8942 9861 11504 12016 Size: 355 1347 196 396 160 919 1643 512 Cuts at: 12016 6488 Size: 9071 Fragments arranged by size: 9071 1643 1347 919 512 396 355 196 160 Bme1580I G_kGCm'C Cuts at: 1790 2273 2716 3776 3923 3954 4093 4132 4327 Size: 483 443 1060 147 31 139 39 195 Cuts at: 4327 4620 4878 5096 6097 6137 6690 7129 7267 Size: 293 258 218 1001 40 553 439 138 Cuts at: 7267 7821 8571 8958 9012 9411 9655 10060 10320 Size: 554 750 387 54 399 244 405 260 Cuts at: 10320 10473 10483 10633 10894 11340 11516 11655 12660 Size: 153 10 150 261 446 176 139 1005 Cuts at: 12660 13816 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1060 1005 1001 750 554 553 483 446 443 439 405 399 387 293 261 260 258 244 218 195 176 153 150 147 139 139 138 54 40 39 31 10 BmgBI CAC'GTC Cuts at: 1318 2731 3388 6418 7036 1318 Size: 1413 657 3030 618 8881 Fragments arranged by size: 8881 3030 1413 657 618 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2643 4314 4583 Size: 416 53 83 399 24 1226 1671 269 Cuts at: 4583 9592 9665 10793 10979 11403 11500 12330 13288 Size: 5009 73 1128 186 424 97 830 958 Cuts at: 13288 442 Size: 1753 Fragments arranged by size: 5009 1753 1671 1226 1128 958 830 424 416 399 269 186 97 83 73 53 24 BmtI G_CTAG'C Cuts at: 1056 8220 1056 Size: 7164 7435 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2674 3052 5812 7741 11336 2674 Size: 378 2760 1929 3595 5937 Fragments arranged by size: 5937 3595 2760 1929 378 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2409 2516 2586 2595 2603 2699 4298 4613 Size: 1170 107 70 9 8 96 1599 315 Cuts at: 4613 4922 4928 5165 5505 5682 5903 6819 7298 Size: 309 6 237 340 177 221 916 479 Cuts at: 7298 7437 7545 7677 7736 7749 8631 9366 9536 Size: 139 108 132 59 13 882 735 170 Cuts at: 9536 10149 10437 10724 11070 11888 13259 1239 Size: 613 288 287 346 818 1371 2579 Fragments arranged by size: 2579 1599 1371 1170 916 882 818 735 613 479 346 340 315 309 288 287 237 221 177 170 139 132 108 107 96 70 59 13 9 8 6 Bpu10I CC'TnA_GC Cuts at: 2463 2619 3027 3245 3366 3441 3630 4118 4290 Size: 156 408 218 121 75 189 488 172 Cuts at: 4290 5944 6030 6231 7472 7823 9618 9931 9970 Size: 1654 86 201 1241 351 1795 313 39 Cuts at: 9970 10697 10908 11378 11743 11836 11842 2463 Size: 727 211 470 365 93 6 5220 Fragments arranged by size: 5220 1795 1654 1241 727 488 470 408 365 351 313 218 211 201 189 172 156 121 93 86 75 39 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3343 3940 3983 6775 9182 11546 12724 13589 13887 Size: 597 43 2792 2407 2364 1178 865 298 Cuts at: 13887 3343 Size: 4055 Fragments arranged by size: 4055 2792 2407 2364 1178 865 597 298 43 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2564 6509 9363 10001 13262 882 Size: 617 1065 3945 2854 638 3261 2219 Fragments arranged by size: 3945 3261 2854 2219 1065 638 617 BsaAI yAC'GTr Cuts at: 493 1204 6703 6850 8654 8668 9412 10507 12390 Size: 711 5499 147 1804 14 744 1095 1883 Cuts at: 12390 493 Size: 2702 Fragments arranged by size: 5499 2702 1883 1804 1095 744 711 147 14 BsaBI GATnn'nnATC Cuts at: 8804 14593 8804 Size: 5789 8810 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2524 3549 5419 6390 Size: 53 83 186 936 991 1025 1870 971 Cuts at: 6390 6927 6941 7068 7689 12406 12788 13423 275 Size: 537 14 127 621 4717 382 635 1451 Fragments arranged by size: 4717 1870 1451 1025 991 971 936 635 621 537 382 186 127 83 53 14 BsaWI w'CCGG_w Cuts at: 2889 3875 13032 13430 13704 13851 14262 2889 Size: 986 9157 398 274 147 411 3226 Fragments arranged by size: 9157 3226 986 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2209 2239 3551 3581 5818 Size: 30 165 30 806 30 1312 30 2237 Cuts at: 5818 5848 6863 6893 7383 7413 8011 8041 8979 Size: 30 1015 30 490 30 598 30 938 Cuts at: 8979 9009 10213 10243 10833 10863 1178 Size: 30 1204 30 590 30 4914 Fragments arranged by size: 4914 2237 1312 1204 1015 938 806 598 590 490 165 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2217 3317 3673 3830 4041 5892 7163 Size: 991 40 1100 356 157 211 1851 1271 Cuts at: 7163 7182 8466 11167 11345 11480 11483 11772 1186 Size: 19 1284 2701 178 135 3 289 4013 Fragments arranged by size: 4013 2701 1851 1284 1271 1100 991 356 289 211 178 157 135 40 19 3 BseYI C'CCAG_C Cuts at: 2017 2101 2108 2135 2519 3031 3777 4621 4808 Size: 84 7 27 384 512 746 844 187 Cuts at: 4808 5989 7721 7772 7814 7925 8643 8767 9623 Size: 1181 1732 51 42 111 718 124 856 Cuts at: 9623 10114 10208 10247 10373 11027 11341 11410 11587 Size: 491 94 39 126 654 314 69 177 Cuts at: 11587 11759 13802 14298 2017 Size: 172 2043 496 2318 Fragments arranged by size: 2318 2043 1732 1181 856 844 746 718 654 512 496 491 384 314 187 177 172 126 124 111 94 84 69 51 42 39 27 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3129 3368 4292 4674 5090 5567 5573 Size: 573 1393 239 924 382 416 477 6 Cuts at: 5573 5786 5951 6290 6302 6603 6683 6720 6770 Size: 213 165 339 12 301 80 37 50 Cuts at: 6770 7815 7827 9434 10529 11328 11370 11876 1163 Size: 1045 12 1607 1095 799 42 506 3886 Fragments arranged by size: 3886 1607 1393 1095 1045 924 799 573 506 477 416 382 339 301 239 213 165 80 50 42 37 12 12 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6556 6821 12049 12810 13838 1540 Size: 275 186 4555 265 5228 761 1028 2301 Fragments arranged by size: 5228 4555 2301 1028 761 275 265 186 BsiHKAI G_wGCw'C Cuts at: 3923 5884 6097 6849 8853 9168 9411 9971 10060 Size: 1961 213 752 2004 315 243 560 89 Cuts at: 10060 10335 11007 11475 12660 12745 13816 3923 Size: 275 672 468 1185 85 1071 4706 Fragments arranged by size: 4706 2004 1961 1185 1071 752 672 560 468 315 275 243 213 89 85 BsmI GAATG_Cn' Cuts at: 8248 8319 12110 12203 8248 Size: 71 3791 93 10644 Fragments arranged by size: 10644 3791 93 71 BsmBI CGTCTCn'nnnn_ Cuts at: 7075 9202 7075 Size: 2127 12472 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2273 2716 3022 3038 3447 3732 Size: 399 264 219 443 306 16 409 285 Cuts at: 3732 3776 3923 3954 4093 4132 4327 4620 4759 Size: 44 147 31 139 39 195 293 139 Cuts at: 4759 4878 4928 5096 5570 5884 6062 6081 6097 Size: 119 50 168 474 314 178 19 16 Cuts at: 6097 6137 6357 6690 6849 7129 7187 7267 7722 Size: 40 220 333 159 280 58 80 455 Cuts at: 7722 7821 8571 8768 8783 8853 8882 8958 9012 Size: 99 750 197 15 70 29 76 54 Cuts at: 9012 9168 9382 9411 9655 9951 9971 10060 10320 Size: 156 214 29 244 296 20 89 260 Cuts at: 10320 10335 10473 10483 10606 10633 10894 11007 11340 Size: 15 138 10 123 27 261 113 333 Cuts at: 11340 11384 11475 11516 11655 11918 12660 12745 13816 Size: 44 91 41 139 263 742 85 1071 Cuts at: 13816 1391 Size: 2174 Fragments arranged by size: 2174 1071 750 742 474 455 443 409 399 333 333 314 306 296 293 285 280 264 263 261 260 244 220 219 214 197 195 178 168 159 156 147 139 139 139 138 123 119 113 99 91 89 85 80 76 70 58 54 50 44 44 41 40 39 31 29 29 27 20 19 16 16 15 15 10 BspEI T'CCGG_A Cuts at: 3875 14262 3875 Size: 10387 4212 BspHI T'CATG_A Cuts at: 5884 7642 9070 12488 14218 5884 Size: 1758 1428 3418 1730 6265 Fragments arranged by size: 6265 3418 1758 1730 1428 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2446 2821 3421 3790 5037 5539 5923 6262 Size: 1602 375 600 369 1247 502 384 339 Cuts at: 6262 6892 8569 9133 9495 10620 10815 11263 11426 Size: 630 1677 564 362 1125 195 448 163 Cuts at: 11426 11508 11954 844 Size: 82 446 3489 Fragments arranged by size: 3489 1677 1602 1247 1125 630 600 564 502 448 446 384 375 369 362 339 195 163 82 BsrBI CCG'CTC Cuts at: 1394 1823 2658 2956 5854 5983 8076 11712 12486 Size: 429 835 298 2898 129 2093 3636 774 Cuts at: 12486 1394 Size: 3507 Fragments arranged by size: 3636 3507 2898 2093 835 774 429 298 129 BsrDI GCAATG_nn' Cuts at: 65 1248 2075 13094 13268 65 Size: 1183 827 11019 174 1396 Fragments arranged by size: 11019 1396 1183 827 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6454 6555 7691 8007 8647 11459 13243 Size: 110 4644 101 1136 316 640 2812 1784 Cuts at: 13243 13430 1700 Size: 187 2869 Fragments arranged by size: 4644 2869 2812 1784 1136 640 316 187 110 101 BsrGI T'GTAC_A Cuts at: 96 5205 5763 6369 96 Size: 5109 558 606 8326 Fragments arranged by size: 8326 5109 606 558 BssHII G'CGCG_C Cuts at: 1925 6211 6213 6252 6314 6473 6587 6599 1925 Size: 4286 2 39 62 159 114 12 9925 Fragments arranged by size: 9925 4286 159 114 62 39 12 2 BssSI C'ACGA_G Cuts at: 2505 3006 9366 9396 9911 10175 12659 13671 2505 Size: 501 6360 30 515 264 2484 1012 3433 Fragments arranged by size: 6360 3433 2484 1012 515 501 264 30 BstAPI GCAn_nnn'nTGC Cuts at: 794 2846 10064 11117 794 Size: 2052 7218 1053 4276 Fragments arranged by size: 7218 4276 2052 1053 BstBI TT'CG_AA Cuts at: 6192 13403 6192 Size: 7211 7388 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 14599 BstXI CCAn_nnnn'nTGG Cuts at: 2124 4267 5338 6683 2124 Size: 2143 1071 1345 10040 Fragments arranged by size: 10040 2143 1345 1071 BstYI r'GATC_y Cuts at: 1972 3499 3878 4148 4498 5326 5731 5971 6721 Size: 1527 379 270 350 828 405 240 750 Cuts at: 6721 7093 8718 8962 11133 11929 12296 12681 12698 Size: 372 1625 244 2171 796 367 385 17 Cuts at: 12698 14150 14236 14248 14594 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2171 1977 1625 1527 1452 828 796 750 405 385 379 372 367 350 346 270 244 240 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3453 3583 3768 4879 5035 6157 7287 9840 10260 Size: 130 185 1111 156 1122 1130 2553 420 Cuts at: 10260 3453 Size: 7792 Fragments arranged by size: 7792 2553 1130 1122 1111 420 185 156 130 BtgI C'CryG_G Cuts at: 513 1065 4005 4064 4153 4830 4984 6081 6206 Size: 552 2940 59 89 677 154 1097 125 Cuts at: 6206 6690 6810 6874 8417 9373 9733 10688 10761 Size: 484 120 64 1543 956 360 955 73 Cuts at: 10761 10824 11065 11191 11476 11625 513 Size: 63 241 126 285 149 3487 Fragments arranged by size: 3487 2940 1543 1097 956 955 677 552 484 360 285 241 154 149 126 125 120 89 73 64 63 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 7872 487 Size: 683 56 6646 7214 Fragments arranged by size: 7214 6646 683 56 BtsI GCAGTG_nn' Cuts at: 2355 2735 3356 3761 4615 4722 4748 5043 5232 Size: 380 621 405 854 107 26 295 189 Cuts at: 5232 5610 5826 7803 8227 9032 9187 9351 10958 Size: 378 216 1977 424 805 155 164 1607 Cuts at: 10958 11346 11619 12115 12909 12929 2355 Size: 388 273 496 794 20 4025 Fragments arranged by size: 4025 1977 1607 854 805 794 621 496 424 405 388 380 378 295 273 216 189 164 155 107 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 14599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 8113 8148 529 Size: 35 7549 35 6980 Fragments arranged by size: 7549 6980 35 35 DraI TTT'AAA Cuts at: 11903 11903 Size: 14599 DraIII CAC_nnn'GTG Cuts at: 5156 5866 5879 5156 Size: 710 13 13876 Fragments arranged by size: 13876 710 13 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 13606 809 Size: 507 12290 1802 Fragments arranged by size: 12290 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2957 3651 Size: 54 1425 50 275 18 450 677 694 Cuts at: 3651 4248 5955 6772 6818 6894 7436 7666 7953 Size: 597 1707 817 46 76 542 230 287 Cuts at: 7953 8121 8134 8748 8977 10149 10489 11098 11163 Size: 168 13 614 229 1172 340 609 65 Cuts at: 11163 11457 11532 11576 12046 12935 14564 8 Size: 294 75 44 470 889 1629 43 Fragments arranged by size: 1707 1629 1425 1172 889 817 694 677 614 609 597 542 470 450 340 294 287 275 230 229 168 76 75 65 54 50 46 44 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 6818 12046 1537 Size: 275 5006 5228 4090 Fragments arranged by size: 5228 5006 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 4517 7125 8117 10573 11028 12529 1353 Size: 689 2475 2608 992 2456 455 1501 3423 Fragments arranged by size: 3423 2608 2475 2456 1501 992 689 455 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8603 13186 13560 13706 387 Size: 1064 138 7014 4583 374 146 1280 Fragments arranged by size: 7014 4583 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 8851 9969 11473 8851 Size: 1118 1504 11977 Fragments arranged by size: 11977 1504 1118 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2409 2516 2586 2595 2603 2699 4298 Size: 393 777 107 70 9 8 96 1599 Cuts at: 4298 4613 4716 4922 4928 5165 5234 5469 5505 Size: 315 103 206 6 237 69 235 36 Cuts at: 5505 5631 5682 5903 6819 7298 7437 7545 7677 Size: 126 51 221 916 479 139 108 132 Cuts at: 7677 7736 7749 7915 7989 8631 8953 9253 9366 Size: 59 13 166 74 642 322 300 113 Cuts at: 9366 9501 9536 10149 10437 10724 11070 11888 12662 Size: 135 35 613 288 287 346 818 774 Cuts at: 12662 13259 14046 14314 1239 Size: 597 787 268 1524 Fragments arranged by size: 1599 1524 916 818 787 777 774 642 613 597 479 393 346 322 315 300 288 287 268 237 235 221 206 166 139 135 132 126 113 108 107 103 96 74 70 69 59 51 36 35 13 9 8 6 EcoNI CCTnn'n_nnAGG Cuts at: 3452 3507 5546 5811 6668 8364 8576 9146 10241 Size: 55 2039 265 857 1696 212 570 1095 Cuts at: 10241 11202 3452 Size: 961 6849 Fragments arranged by size: 6849 2039 1696 1095 961 857 570 265 212 55 EcoO109I rG'GnC_Cy Cuts at: 2058 2712 2719 3870 3950 3951 4874 4875 5059 Size: 654 7 1151 80 1 923 1 184 Cuts at: 5059 5092 5093 5408 5665 5986 6715 6904 7126 Size: 33 1 315 257 321 729 189 222 Cuts at: 7126 7297 7381 7617 7818 7922 8093 8567 8568 Size: 171 84 236 201 104 171 474 1 Cuts at: 8568 8921 8955 9008 9009 9291 9644 10256 10721 Size: 353 34 53 1 282 353 612 465 Cuts at: 10721 11054 11336 11337 11831 2058 Size: 333 282 1 494 4826 Fragments arranged by size: 4826 1151 923 729 654 612 494 474 465 353 353 333 321 315 282 282 257 236 222 201 189 184 171 171 104 84 80 53 34 33 7 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 11908 12286 11908 Size: 378 14221 EcoRV GAT'ATC Cuts at: 10768 10768 Size: 14599 FspI TGC'GCA Cuts at: 7739 13105 7739 Size: 5366 9233 HaeII r_GCGC'y Cuts at: 1678 3552 6074 6166 6332 6338 6368 6461 6488 Size: 1874 2522 92 166 6 30 93 27 Cuts at: 6488 6495 6806 6944 7692 13746 1678 Size: 7 311 138 748 6054 2531 Fragments arranged by size: 6054 2531 2522 1874 748 311 166 138 93 92 30 27 7 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3332 3364 3566 3598 4133 Size: 32 135 32 1760 32 202 32 535 Cuts at: 4133 4165 4202 4234 4320 4352 4514 4546 4754 Size: 32 37 32 86 32 162 32 208 Cuts at: 4754 4786 4910 4942 5018 5050 5387 5419 5518 Size: 32 124 32 76 32 337 32 99 Cuts at: 5518 5550 6140 6172 6863 6895 7169 7201 7252 Size: 32 590 32 691 32 274 32 51 Cuts at: 7252 7284 7383 7415 8091 8123 8591 8623 8900 Size: 32 99 32 676 32 468 32 277 Cuts at: 8900 8932 8979 9011 9200 9232 9953 9985 10000 Size: 32 47 32 189 32 721 32 15 Cuts at: 10000 10032 13240 13272 13314 13346 1373 Size: 32 3208 32 42 32 2626 Fragments arranged by size: 3208 2626 1760 721 691 676 590 535 468 337 277 274 208 202 189 162 135 124 99 99 86 76 51 47 42 37 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 3400 4230 4780 6931 7200 9277 Size: 988 281 1462 830 550 2151 269 2077 Cuts at: 9277 11943 12189 669 Size: 2666 246 3079 Fragments arranged by size: 3079 2666 2151 2077 1462 988 830 550 281 269 246 HindIII A'AGCT_T Cuts at: 748 3958 7081 10342 748 Size: 3210 3123 3261 5005 Fragments arranged by size: 5005 3261 3210 3123 HpaI GTT'AAC Cuts at: 4780 7200 12189 4780 Size: 2420 4989 7190 Fragments arranged by size: 7190 4989 2420 KasI G'GCGC_C Cuts at: 3548 6940 7688 3548 Size: 3392 748 10459 Fragments arranged by size: 10459 3392 748 KpnI G_GTAC'C Cuts at: 2462 5684 11924 2462 Size: 3222 6240 5137 Fragments arranged by size: 6240 5137 3222 MfeI C'AATT_G Cuts at: 10083 12198 10083 Size: 2115 12484 MluI A'CGCG_T Cuts at: 6655 6739 14475 6655 Size: 84 7736 6779 Fragments arranged by size: 7736 6779 84 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3603 4566 6366 6804 8152 9835 10221 11548 Size: 2298 963 1800 438 1348 1683 386 1327 Cuts at: 11548 13713 13897 1305 Size: 2165 184 2007 Fragments arranged by size: 2298 2165 2007 1800 1683 1348 1327 963 438 386 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 3653 4250 7668 7955 Size: 54 1425 343 450 1371 597 3418 287 Cuts at: 7955 8123 8750 10491 11165 11534 10 Size: 168 627 1741 674 369 3075 Fragments arranged by size: 3418 3075 1741 1425 1371 674 627 597 450 369 343 287 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 3170 3207 3909 4112 4427 6110 7832 Size: 648 2004 37 702 203 315 1683 1722 Cuts at: 7832 7850 8039 8159 9305 9465 10145 10175 11094 Size: 18 189 120 1146 160 680 30 919 Cuts at: 11094 11624 12557 12916 13075 518 Size: 530 933 359 159 2042 Fragments arranged by size: 2042 2004 1722 1683 1146 933 919 702 680 648 530 359 315 203 189 160 159 120 37 30 18 MspA1I CmG'CkG Cuts at: 1606 2174 2262 2338 2677 2977 3640 3980 4112 Size: 568 88 76 339 300 663 340 132 Cuts at: 4112 4276 5443 5923 5993 6029 6083 6325 6692 Size: 164 1167 480 70 36 54 242 367 Cuts at: 6692 7471 7745 7811 9043 9049 9418 9554 9601 Size: 779 274 66 1232 6 369 136 47 Cuts at: 9601 9728 10641 11675 11747 12015 12692 13840 14085 Size: 127 913 1034 72 268 677 1148 245 Cuts at: 14085 1606 Size: 2120 Fragments arranged by size: 2120 1232 1167 1148 1034 913 779 677 663 568 480 369 367 340 339 300 274 268 245 242 164 136 132 127 88 76 72 70 66 54 47 36 6 NaeI GCC'GGC Cuts at: 6456 8649 11461 6456 Size: 2193 2812 9594 Fragments arranged by size: 9594 2812 2193 NarI GG'CG_CC Cuts at: 3549 6941 7689 3549 Size: 3392 748 10459 Fragments arranged by size: 10459 3392 748 NcoI C'CATG_G Cuts at: 513 1065 4005 4153 513 Size: 552 2940 148 10959 Fragments arranged by size: 10959 2940 552 148 NdeI CA'TA_TG Cuts at: 387 387 Size: 14599 NgoMIV G'CCGG_C Cuts at: 6454 8647 11459 6454 Size: 2193 2812 9594 Fragments arranged by size: 9594 2812 2193 NheI G'CTAG_C Cuts at: 1052 8216 1052 Size: 7164 7435 NotI GC'GGCC_GC Cuts at: 12046 12046 Size: 14599 NspI r_CATG'y Cuts at: 1132 1229 3059 5600 6302 7208 9875 10193 11957 Size: 97 1830 2541 702 906 2667 318 1764 Cuts at: 11957 13502 1132 Size: 1545 2229 Fragments arranged by size: 2667 2541 2229 1830 1764 1545 906 702 318 97 PasI CC'CwG_GG Cuts at: 1348 2536 4730 5363 8252 8274 8844 11827 1348 Size: 1188 2194 633 2889 22 570 2983 4120 Fragments arranged by size: 4120 2983 2889 2194 1188 633 570 22 PciI A'CATG_T Cuts at: 13498 13498 Size: 14599 PflMI CCAn_nnn'nTGG Cuts at: 1108 2791 2992 3188 4177 4900 5156 5509 5926 Size: 1683 201 196 989 723 256 353 417 Cuts at: 5926 7256 8030 8533 9997 10498 11048 11417 11530 Size: 1330 774 503 1464 501 550 369 113 Cuts at: 11530 1108 Size: 4177 Fragments arranged by size: 4177 1683 1464 1330 989 774 723 550 503 501 417 369 353 256 201 196 113 PfoI T'CCnGG_A Cuts at: 5698 8706 10092 10736 5698 Size: 3008 1386 644 9561 Fragments arranged by size: 9561 3008 1386 644 PmeI GTTT'AAAC Cuts at: 11903 11903 Size: 14599 PmlI CAC'GTG Cuts at: 6703 8654 9412 10507 6703 Size: 1951 758 1095 10795 Fragments arranged by size: 10795 1951 1095 758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 12668 12700 14181 14213 12668 Size: 32 1481 32 13054 Fragments arranged by size: 13054 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3870 5059 5408 5986 6715 6904 7297 7381 7617 Size: 1189 349 578 729 189 393 84 236 Cuts at: 7617 8921 9291 10256 11054 11831 3870 Size: 1304 370 965 798 777 6638 Fragments arranged by size: 6638 1304 1189 965 798 777 729 578 393 370 349 236 189 84 PshAI GACnn'nnGTC Cuts at: 1613 3868 1613 Size: 2255 12344 PsiI TTA'TAA Cuts at: 12169 12169 Size: 14599 PspOMI G'GGCC_C Cuts at: 2712 3950 4874 5092 6686 7125 7817 8567 8954 Size: 1238 924 218 1594 439 692 750 387 Cuts at: 8954 9008 10890 11336 2712 Size: 54 1882 446 5975 Fragments arranged by size: 5975 1882 1594 1238 924 750 692 446 439 387 218 54 PstI C_TGCA'G Cuts at: 830 2362 2457 2949 3801 5643 5724 5833 7615 Size: 1532 95 492 852 1842 81 109 1782 Cuts at: 7615 7632 7750 8181 8439 8580 9048 9423 9727 Size: 17 118 431 258 141 468 375 304 Cuts at: 9727 10965 11122 11680 11951 830 Size: 1238 157 558 271 3478 Fragments arranged by size: 3478 1842 1782 1532 1238 852 558 492 468 431 375 304 271 258 157 141 118 109 95 81 17 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 14599 PvuII CAG'CTG Cuts at: 2677 3640 3980 4112 4276 5443 5923 6029 7471 Size: 963 340 132 164 1167 480 106 1442 Cuts at: 7471 7745 7811 9043 9049 9418 9601 9728 11675 Size: 274 66 1232 6 369 183 127 1947 Cuts at: 11675 11747 2677 Size: 72 5529 Fragments arranged by size: 5529 1947 1442 1232 1167 963 480 369 340 274 183 164 132 127 106 72 66 6 SacI G_AGCT'C Cuts at: 8853 9971 11475 8853 Size: 1118 1504 11977 Fragments arranged by size: 11977 1504 1118 SacII CC_GC'GG Cuts at: 6084 6693 6084 Size: 609 13990 SalI G'TCGA_C Cuts at: 1936 11941 1936 Size: 10005 4594 SanDI GG'GwC_CC Cuts at: 5986 6715 7381 7617 9291 11831 5986 Size: 729 666 236 1674 2540 8754 Fragments arranged by size: 8754 2540 1674 729 666 236 SapI GCTCTTCn'nnn_ Cuts at: 2042 2042 Size: 14599 SbfI CC_TGCA'GG Cuts at: 2457 3801 5643 7615 11951 2457 Size: 1344 1842 1972 4336 5105 Fragments arranged by size: 5105 4336 1972 1842 1344 ScaI AGT'ACT Cuts at: 12847 14559 12847 Size: 1712 12887 SexAI A'CCwGG_T Cuts at: 4456 9147 11267 4456 Size: 4691 2120 7788 Fragments arranged by size: 7788 4691 2120 SfcI C'TryA_G Cuts at: 826 1045 2358 2453 2672 2945 3797 4307 4382 Size: 219 1313 95 219 273 852 510 75 Cuts at: 4382 4632 5428 5639 5720 5829 7611 7628 7634 Size: 250 796 211 81 109 1782 17 6 Cuts at: 7634 7746 8082 8177 8314 8435 8576 8626 9044 Size: 112 336 95 137 121 141 50 418 Cuts at: 9044 9419 9522 9562 9723 10596 10961 11118 11323 Size: 375 103 40 161 873 365 157 205 Cuts at: 11323 11676 11947 13082 13763 13954 826 Size: 353 271 1135 681 191 1471 Fragments arranged by size: 1782 1471 1313 1135 873 852 796 681 510 418 375 365 353 336 273 271 250 219 219 211 205 191 161 157 141 137 121 112 109 103 95 95 81 75 50 40 17 6 SfiI GGCCn_nnn'nGGCC Cuts at: 3834 4256 4873 9906 11171 3834 Size: 422 617 5033 1265 7262 Fragments arranged by size: 7262 5033 1265 617 422 SfoI GGC'GCC Cuts at: 3550 6942 7690 3550 Size: 3392 748 10459 Fragments arranged by size: 10459 3392 748 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 14599 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 14599 SmaI CCC'GGG Cuts at: 1872 4916 6713 7487 8213 9650 11684 11926 1872 Size: 3044 1797 774 726 1437 2034 242 4545 Fragments arranged by size: 4545 3044 2034 1797 1437 774 726 242 SmlI C'TyrA_G Cuts at: 820 1017 1943 3322 3955 3998 6790 9161 11561 Size: 197 926 1379 633 43 2792 2371 2400 Cuts at: 11561 12703 13604 13866 820 Size: 1142 901 262 1553 Fragments arranged by size: 2792 2400 2371 1553 1379 1142 926 901 633 262 197 43 SnaBI TAC'GTA Cuts at: 493 12390 493 Size: 11897 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 14599 SphI G_CATG'C Cuts at: 1132 6302 10193 11957 1132 Size: 5170 3891 1764 3774 Fragments arranged by size: 5170 3891 3774 1764 SrfI GCCC'GGGC Cuts at: 11684 11684 Size: 14599 SspI AAT'ATT Cuts at: 5 52 12523 5 Size: 47 12471 2081 Fragments arranged by size: 12471 2081 47 StuI AGG'CCT Cuts at: 1517 2684 3996 4867 5462 9881 1517 Size: 1167 1312 871 595 4419 6235 Fragments arranged by size: 6235 4419 1312 1167 871 595 StyI C'CwwG_G Cuts at: 513 1065 3508 3852 4005 4153 4206 4245 5890 Size: 552 2443 344 153 148 53 39 1645 Cuts at: 5890 7752 8559 9321 10836 10876 11076 12038 513 Size: 1862 807 762 1515 40 200 962 3074 Fragments arranged by size: 3074 2443 1862 1645 1515 962 807 762 552 344 200 153 148 53 40 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2661 3266 3572 3791 3845 4381 5148 Size: 1255 1081 605 306 219 54 536 767 Cuts at: 5148 5158 5412 6080 6525 7891 10349 10787 11404 Size: 10 254 668 445 1366 2458 438 617 Cuts at: 11404 11649 12626 12643 12796 12981 13426 325 Size: 245 977 17 153 185 445 1498 Fragments arranged by size: 2458 1498 1366 1255 1081 977 767 668 617 605 536 445 445 438 306 254 245 219 185 153 54 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 5205 5763 Size: 274 80 33 51 1443 258 2970 558 Cuts at: 5763 6369 6963 8363 9705 9778 10033 12845 14557 Size: 606 594 1400 1342 73 255 2812 1712 Cuts at: 14557 96 Size: 138 Fragments arranged by size: 2970 2812 1712 1443 1400 1342 606 594 558 274 258 255 138 80 73 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2209 2241 3551 3583 5694 5726 5818 5850 7866 Size: 32 1310 32 2111 32 92 32 2016 Cuts at: 7866 7898 8011 8043 9236 9268 10440 10472 10833 Size: 32 113 32 1193 32 1172 32 361 Cuts at: 10833 10865 14559 14591 2209 Size: 32 3694 32 2217 Fragments arranged by size: 3694 2217 2111 2016 1310 1193 1172 361 113 92 32 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3260 1316 Size: 1944 12655 XbaI T'CTAG_A Cuts at: 9682 11935 9682 Size: 2253 12346 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3515 3697 4151 4178 4327 5084 5157 5245 5480 Size: 182 454 27 149 757 73 88 235 Cuts at: 5480 8651 8704 11359 3515 Size: 3171 53 2655 6755 Fragments arranged by size: 6755 3171 2655 757 454 235 182 149 88 73 53 27 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 14599 XmaI C'CCGG_G Cuts at: 1870 4914 6711 7485 8211 9648 11682 11924 1870 Size: 3044 1797 774 726 1437 2034 242 4545 Fragments arranged by size: 4545 3044 2034 1797 1437 774 726 242 XmnI GAAnn'nnTTC Cuts at: 12728 12728 Size: 14599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5420 6928 12407 276 Size: 53 83 186 936 3886 1508 5479 2468 Fragments arranged by size: 5479 3886 2468 1508 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BfrBI BsiWI BstZ17I FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SwaI