Restriction map for
pFN21AA1773
(Linear) (Six-base) MAPSORT of: KIAA1773.seq Check: 3310 from: 1 to: 9894
KIAA1773.orf
With 182 enzymes: SgfI *
April 9, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 816 1416 1785 3534 3918 4257 6564 7128
Size: 816 600 369 1749 384 339 2307 564
Cuts at: 7128 9258 9421 9894
Size: 2130 163 473
Fragments arranged by size:
2307 2130 1749 816 600 564 473 384
369 339 163
AatII G_ACGT'C
Cuts at: 0 3417 4925 9894
Size: 3417 1508 4969
Fragments arranged by size:
4969 3417 1508
AccI GT'mk_AC
Cuts at: 0 6665 9894
Size: 6665 3229
Acc65I G'GTAC_C
Cuts at: 0 453 3675 9894
Size: 453 3222 6219
Fragments arranged by size:
6219 3222 453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2711 3229 3464 3626 5910 5984 6948 7248
Size: 2711 518 235 162 2284 74 964 300
Cuts at: 7248 7496 9894
Size: 248 2398
Fragments arranged by size:
2711 2398 2284 964 518 300 248 235
162 74
AfeI AGC'GCT
Cuts at: 0 4361 4488 4799 9894
Size: 4361 127 311 5095
Fragments arranged by size:
5095 4361 311 127
AflIII A'CryG_T
Cuts at: 0 4650 4734 9894
Size: 4650 84 5160
Fragments arranged by size:
5160 4650 84
AleI CACnn'nnGTG
Cuts at: 0 2107 2422 4105 5827 6034 6154 7300 7460
Size: 2107 315 1683 1722 207 120 1146 160
Cuts at: 7460 8140 8170 9619 9894
Size: 680 30 1449 275
Fragments arranged by size:
2107 1722 1683 1449 1146 680 315 275
207 160 120 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2125 2626 5017 7671 9894
Size: 2125 501 2391 2654 2223
Fragments arranged by size:
2654 2391 2223 2125 501
AlwNI CAG_nnn'CTG
Cuts at: 0 514 613 717 3057 3226 3537 3586 3649
Size: 514 99 104 2340 169 311 49 63
Cuts at: 3649 3921 4000 4024 4902 5809 5833 7047 7525
Size: 272 79 24 878 907 24 1214 478
Cuts at: 7525 7717 7765 7834 8254 8386 8749 8914 8964
Size: 192 48 69 420 132 363 165 50
Cuts at: 8964 9043 9112 9343 9856 9894
Size: 79 69 231 513 38
Fragments arranged by size:
2340 1214 907 878 514 513 478 420
363 311 272 231 192 169 165 132
104 99 79 79 69 69 63 50
49 48 38 24 24
ApaI G_GGCC'C
Cuts at: 0 711 1949 2873 3091 4685 5124 5816 6566
Size: 711 1238 924 218 1594 439 692 750
Cuts at: 6566 6953 7007 8889 9335 9894
Size: 387 54 1882 446 559
Fragments arranged by size:
1882 1594 1238 924 750 711 692 559
446 439 387 218 54
ApaLI G'TGCA_C
Cuts at: 0 1914 4088 7402 8051 9894
Size: 1914 2174 3314 649 1843
Fragments arranged by size:
3314 2174 1914 1843 649
AvaI C'yCGr_G
Cuts at: 0 437 1490 2321 2498 2633 2909 4123 4706
Size: 437 1053 831 177 135 276 1214 583
Cuts at: 4706 5480 6092 6206 7437 7643 7808 9677 9894
Size: 774 612 114 1231 206 165 1869 217
Fragments arranged by size:
1869 1231 1214 1053 831 774 612 583
437 276 217 206 177 165 135 114
AvrII C'CTAG_G
Cuts at: 0 8871 9894
Size: 8871 1023
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 593 626 9894
Size: 593 33 9268
Fragments arranged by size:
9268 593 33
BamHI G'GATC_C
Cuts at: 0 1494 2143 2493 3321 3726 5088 6957 9894
Size: 1494 649 350 828 405 1362 1869 2937
Fragments arranged by size:
2937 1869 1494 1362 828 649 405 350
BanI G'GyrC_C
Cuts at: 0 108 115 370 453 475 1507 1543 1768
Size: 108 7 255 83 22 1032 36 225
Cuts at: 1768 2409 2416 2463 3034 3675 3990 4114 4129
Size: 641 7 47 571 641 315 124 15
Cuts at: 4129 4935 5277 5422 5589 5683 5980 6364 7216
Size: 806 342 145 167 94 297 384 852
Cuts at: 7216 7969 8067 8442 8473 8527 8625 8787 9506
Size: 753 98 375 31 54 98 162 719
Cuts at: 9506 9894
Size: 388
Fragments arranged by size:
1032 852 806 753 719 641 641 571
388 384 375 342 315 297 255 225
167 162 145 124 108 98 98 94
83 54 47 36 31 22 15 7
7
BanII G_rGCy'C
Cuts at: 0 49 711 1017 1033 1442 1727 1949 2754
Size: 49 662 306 16 409 285 222 805
Cuts at: 2754 2873 2923 3091 3565 4057 4076 4352 4685
Size: 119 50 168 474 492 19 276 333
Cuts at: 4685 5124 5182 5717 5816 6566 6763 6778 6848
Size: 439 58 535 99 750 197 15 70
Cuts at: 6848 6877 6953 7007 7377 7946 7966 8601 8889
Size: 29 76 54 370 569 20 635 288
Cuts at: 8889 9335 9379 9470 9894
Size: 446 44 91 424
Fragments arranged by size:
805 750 662 635 569 535 492 474
446 439 424 409 370 333 306 288
285 276 222 197 168 119 99 91
76 70 58 54 50 49 44 29
20 19 16 15
BbeI G_GCGC'C
Cuts at: 0 1547 4939 5687 9894
Size: 1547 3392 748 4207
Fragments arranged by size:
4207 3392 1547 748
BbsI GAAGACnn'nnnn_
Cuts at: 0 2699 3452 4593 4626 7484 7581 9528 9894
Size: 2699 753 1141 33 2858 97 1947 366
Fragments arranged by size:
2858 2699 1947 1141 753 366 97 33
BbvCI CC'TCA_GC
Cuts at: 0 1361 1625 2113 2285 3939 4025 4226 5467
Size: 1361 264 488 172 1654 86 201 1241
Cuts at: 5467 7613 8692 8903 9738 9831 9837 9894
Size: 2146 1079 211 835 93 6 57
Fragments arranged by size:
2146 1654 1361 1241 1079 835 488 264
211 201 172 93 86 57 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 175 209 2527 2561 8182 8216 9232 9266
Size: 175 34 2318 34 5621 34 1016 34
Cuts at: 9266 9894
Size: 628
Fragments arranged by size:
5621 2318 1016 628 175 34 34 34
34
BclI T'GATC_A
Cuts at: 0 6158 8100 9137 9894
Size: 6158 1942 1037 757
Fragments arranged by size:
6158 1942 1037 757
BglI GCCn_nnn'nGGC
Cuts at: 0 1829 2251 2868 3134 4680 7901 9166 9451
Size: 1829 422 617 266 1546 3221 1265 285
Cuts at: 9451 9725 9894
Size: 274 169
Fragments arranged by size:
3221 1829 1546 1265 617 422 285 274
266 169
BglII A'GATC_T
Cuts at: 0 3966 6713 9894
Size: 3966 2747 3181
Fragments arranged by size:
3966 3181 2747
BlpI GC'TnA_GC
Cuts at: 0 4483 4838 6185 6381 6777 6937 7856 9499
Size: 4483 355 1347 196 396 160 919 1643
Cuts at: 9499 9894
Size: 395
Fragments arranged by size:
4483 1643 1347 919 396 395 355 196
160
Bme1580I G_kGCm'C
Cuts at: 0 268 711 1771 1918 1949 2088 2127 2322
Size: 268 443 1060 147 31 139 39 195
Cuts at: 2322 2615 2873 3091 4092 4132 4685 5124 5262
Size: 293 258 218 1001 40 553 439 138
Cuts at: 5262 5816 6566 6953 7007 7406 7650 8055 8315
Size: 554 750 387 54 399 244 405 260
Cuts at: 8315 8468 8478 8628 8889 9335 9511 9650 9894
Size: 153 10 150 261 446 176 139 244
Fragments arranged by size:
1060 1001 750 554 553 446 443 439
405 399 387 293 268 261 260 258
244 244 218 195 176 153 150 147
139 139 138 54 40 39 31 10
BmgBI CAC'GTC
Cuts at: 0 726 1383 4413 5031 9894
Size: 726 657 3030 618 4863
Fragments arranged by size:
4863 3030 726 657 618
BmrI ACTGGGnnnn_n'
Cuts at: 0 638 2309 2578 7587 7660 8788 8974 9398
Size: 638 1671 269 5009 73 1128 186 424
Cuts at: 9398 9495 9894
Size: 97 399
Fragments arranged by size:
5009 1671 1128 638 424 399 269 186
97 73
BmtI G_CTAG'C
Cuts at: 0 6215 9894
Size: 6215 3679
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 669 1047 3807 5736 9331 9894
Size: 669 378 2760 1929 3595 563
Fragments arranged by size:
3595 2760 1929 669 563 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 511 581 590 598 694 2293 2608
Size: 404 107 70 9 8 96 1599 315
Cuts at: 2608 2917 2923 3160 3500 3677 3898 4814 5293
Size: 309 6 237 340 177 221 916 479
Cuts at: 5293 5432 5540 5672 5731 5744 6626 7361 7531
Size: 139 108 132 59 13 882 735 170
Cuts at: 7531 8144 8432 8719 9065 9883 9894
Size: 613 288 287 346 818 11
Fragments arranged by size:
1599 916 882 818 735 613 479 404
346 340 315 309 288 287 237 221
177 170 139 132 108 107 96 70
59 13 11 9 8 6
Bpu10I CC'TnA_GC
Cuts at: 0 458 614 1022 1240 1361 1436 1625 2113
Size: 458 156 408 218 121 75 189 488
Cuts at: 2113 2285 3939 4025 4226 5467 5818 7613 7926
Size: 172 1654 86 201 1241 351 1795 313
Cuts at: 7926 7965 8692 8903 9373 9738 9831 9837 9894
Size: 39 727 211 470 365 93 6 57
Fragments arranged by size:
1795 1654 1241 727 488 470 458 408
365 351 313 218 211 201 189 172
156 121 93 86 75 57 39 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1338 1935 1978 4770 7177 9541 9894
Size: 1338 597 43 2792 2407 2364 353
Fragments arranged by size:
2792 2407 2364 1338 597 353 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 559 4504 7358 7996 9894
Size: 559 3945 2854 638 1898
Fragments arranged by size:
3945 2854 1898 638 559
BsaAI yAC'GTr
Cuts at: 0 4698 4845 6649 6663 7407 8502 9894
Size: 4698 147 1804 14 744 1095 1392
Fragments arranged by size:
4698 1804 1392 1095 744 147 14
BsaBI GATnn'nnATC
Cuts at: 0 6799 9894
Size: 6799 3095
BsaHI Gr'CG_yC
Cuts at: 0 519 1544 3414 4385 4922 4936 5063 5684
Size: 519 1025 1870 971 537 14 127 621
Cuts at: 5684 9894
Size: 4210
Fragments arranged by size:
4210 1870 1025 971 621 537 519 127
14
BsaWI w'CCGG_w
Cuts at: 0 884 1870 9894
Size: 884 986 8024
Fragments arranged by size:
8024 986 884
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 204 234 1546 1576 3813 3843 4858 4888
Size: 204 30 1312 30 2237 30 1015 30
Cuts at: 4888 5378 5408 6006 6036 6974 7004 8208 8238
Size: 490 30 598 30 938 30 1204 30
Cuts at: 8238 8828 8858 9894
Size: 590 30 1036
Fragments arranged by size:
2237 1312 1204 1036 1015 938 598 590
490 204 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 212 1312 1668 1825 2036 3887 5158
Size: 172 40 1100 356 157 211 1851 1271
Cuts at: 5158 5177 6461 9162 9340 9475 9478 9767 9894
Size: 19 1284 2701 178 135 3 289 127
Fragments arranged by size:
2701 1851 1284 1271 1100 356 289 211
178 172 157 135 127 40 19 3
BseYI C'CCAG_C
Cuts at: 0 12 96 103 130 514 1026 1772 2616
Size: 12 84 7 27 384 512 746 844
Cuts at: 2616 2803 3984 5716 5767 5809 5920 6638 6762
Size: 187 1181 1732 51 42 111 718 124
Cuts at: 6762 7618 8109 8203 8242 8368 9022 9336 9405
Size: 856 491 94 39 126 654 314 69
Cuts at: 9405 9582 9754 9894
Size: 177 172 140
Fragments arranged by size:
1732 1181 856 844 746 718 654 512
491 384 314 187 177 172 140 126
124 111 94 84 69 51 42 39
27 12 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 1363 2287 2669 3085 3562 3568 3781
Size: 1124 239 924 382 416 477 6 213
Cuts at: 3781 3946 4285 4297 4598 4678 4715 4765 5810
Size: 165 339 12 301 80 37 50 1045
Cuts at: 5810 5822 7429 8524 9323 9365 9871 9894
Size: 12 1607 1095 799 42 506 23
Fragments arranged by size:
1607 1124 1095 1045 924 799 506 477
416 382 339 301 239 213 165 80
50 42 37 23 12 12 6
BsiEI CG_ry'CG
Cuts at: 0 4551 4816 9894
Size: 4551 265 5078
Fragments arranged by size:
5078 4551 265
BsiHKAI G_wGCw'C
Cuts at: 0 1918 3879 4092 4844 6848 7163 7406 7966
Size: 1918 1961 213 752 2004 315 243 560
Cuts at: 7966 8055 8330 9002 9470 9894
Size: 89 275 672 468 424
Fragments arranged by size:
2004 1961 1918 752 672 560 468 424
315 275 243 213 89
BsmI GAATG_Cn'
Cuts at: 0 6243 6314 9894
Size: 6243 71 3580
Fragments arranged by size:
6243 3580 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5070 7197 9894
Size: 5070 2127 2697
Fragments arranged by size:
5070 2697 2127
Bsp1286I G_dGCh'C
Cuts at: 0 49 268 711 1017 1033 1442 1727 1771
Size: 49 219 443 306 16 409 285 44
Cuts at: 1771 1918 1949 2088 2127 2322 2615 2754 2873
Size: 147 31 139 39 195 293 139 119
Cuts at: 2873 2923 3091 3565 3879 4057 4076 4092 4132
Size: 50 168 474 314 178 19 16 40
Cuts at: 4132 4352 4685 4844 5124 5182 5262 5717 5816
Size: 220 333 159 280 58 80 455 99
Cuts at: 5816 6566 6763 6778 6848 6877 6953 7007 7163
Size: 750 197 15 70 29 76 54 156
Cuts at: 7163 7377 7406 7650 7946 7966 8055 8315 8330
Size: 214 29 244 296 20 89 260 15
Cuts at: 8330 8468 8478 8601 8628 8889 9002 9335 9379
Size: 138 10 123 27 261 113 333 44
Cuts at: 9379 9470 9511 9650 9894
Size: 91 41 139 244
Fragments arranged by size:
750 474 455 443 409 333 333 314
306 296 293 285 280 261 260 244
244 220 219 214 197 195 178 168
159 156 147 139 139 139 138 123
119 113 99 91 89 80 76 70
58 54 50 49 44 44 41 40
39 31 29 29 27 20 19 16
16 15 15 10
BspEI T'CCGG_A
Cuts at: 0 1870 9894
Size: 1870 8024
BspHI T'CATG_A
Cuts at: 0 3879 5637 7065 9894
Size: 3879 1758 1428 2829
Fragments arranged by size:
3879 2829 1758 1428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 441 816 1416 1785 3032 3534 3918 4257
Size: 441 375 600 369 1247 502 384 339
Cuts at: 4257 4887 6564 7128 7490 8615 8810 9258 9421
Size: 630 1677 564 362 1125 195 448 163
Cuts at: 9421 9503 9894
Size: 82 391
Fragments arranged by size:
1677 1247 1125 630 600 564 502 448
441 391 384 375 369 362 339 195
163 82
BsrBI CCG'CTC
Cuts at: 0 653 951 3849 3978 6071 9707 9894
Size: 653 298 2898 129 2093 3636 187
Fragments arranged by size:
3636 2898 2093 653 298 187 129
BsrDI GCAATG_nn'
Cuts at: 0 70 9894
Size: 70 9824
BsrFI r'CCGG_y
Cuts at: 0 4449 4550 5686 6002 6642 9454 9894
Size: 4449 101 1136 316 640 2812 440
Fragments arranged by size:
4449 2812 1136 640 440 316 101
BsrGI T'GTAC_A
Cuts at: 0 3200 3758 4364 9894
Size: 3200 558 606 5530
Fragments arranged by size:
5530 3200 606 558
BssHII G'CGCG_C
Cuts at: 0 4206 4208 4247 4309 4468 4582 4594 9894
Size: 4206 2 39 62 159 114 12 5300
Fragments arranged by size:
5300 4206 159 114 62 39 12 2
BssSI C'ACGA_G
Cuts at: 0 500 1001 7361 7391 7906 8170 9894
Size: 500 501 6360 30 515 264 1724
Fragments arranged by size:
6360 1724 515 501 500 264 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 841 8059 9112 9894
Size: 841 7218 1053 782
Fragments arranged by size:
7218 1053 841 782
BstBI TT'CG_AA
Cuts at: 0 4187 9894
Size: 4187 5707
BstXI CCAn_nnnn'nTGG
Cuts at: 0 119 2262 3333 4678 9894
Size: 119 2143 1071 1345 5216
Fragments arranged by size:
5216 2143 1345 1071 119
BstYI r'GATC_y
Cuts at: 0 1494 1873 2143 2493 3321 3726 3966 4716
Size: 1494 379 270 350 828 405 240 750
Cuts at: 4716 5088 6713 6957 9128 9894
Size: 372 1625 244 2171 766
Fragments arranged by size:
2171 1625 1494 828 766 750 405 379
372 350 270 244 240
Bsu36I CC'TnA_GG
Cuts at: 0 1448 1578 1763 2874 3030 4152 5282 7835
Size: 1448 130 185 1111 156 1122 1130 2553
Cuts at: 7835 8255 9894
Size: 420 1639
Fragments arranged by size:
2553 1639 1448 1130 1122 1111 420 185
156 130
BtgI C'CryG_G
Cuts at: 0 2000 2059 2148 2825 2979 4076 4201 4685
Size: 2000 59 89 677 154 1097 125 484
Cuts at: 4685 4805 4869 6412 7368 7728 8683 8756 8819
Size: 120 64 1543 956 360 955 73 63
Cuts at: 8819 9060 9186 9471 9620 9894
Size: 241 126 285 149 274
Fragments arranged by size:
2000 1543 1097 956 955 677 484 360
285 274 241 154 149 126 125 120
89 73 64 63 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5867 9894
Size: 5867 4027
BtsI GCAGTG_nn'
Cuts at: 0 350 730 1351 1756 2610 2717 2743 3038
Size: 350 380 621 405 854 107 26 295
Cuts at: 3038 3227 3605 3821 5798 6222 7027 7182 7346
Size: 189 378 216 1977 424 805 155 164
Cuts at: 7346 8953 9341 9614 9894
Size: 1607 388 273 280
Fragments arranged by size:
1977 1607 854 805 621 424 405 388
380 378 350 295 280 273 216 189
164 155 107 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6108 6143 9894
Size: 6108 35 3751
Fragments arranged by size:
6108 3751 35
DraIII CAC_nnn'GTG
Cuts at: 0 3151 3861 3874 9894
Size: 3151 710 13 6020
Fragments arranged by size:
6020 3151 710 13
EaeI y'GGCC_r
Cuts at: 0 275 952 1646 2243 3950 4767 4813 4889
Size: 275 677 694 597 1707 817 46 76
Cuts at: 4889 5431 5661 5948 6116 6129 6743 6972 8144
Size: 542 230 287 168 13 614 229 1172
Cuts at: 8144 8484 9093 9158 9452 9527 9571 9894
Size: 340 609 65 294 75 44 323
Fragments arranged by size:
1707 1172 817 694 677 614 609 597
542 340 323 294 287 275 230 229
168 76 75 65 46 44 13
EagI C'GGCC_G
Cuts at: 0 4813 9894
Size: 4813 5081
EarI CTCTTCn'nnn_
Cuts at: 0 37 2512 5120 6112 8568 9023 9894
Size: 37 2475 2608 992 2456 455 871
Fragments arranged by size:
2608 2475 2456 992 871 455 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 6598 9894
Size: 6598 3296
EcoICRI GAG'CTC
Cuts at: 0 6846 7964 9468 9894
Size: 6846 1118 1504 426
Fragments arranged by size:
6846 1504 1118 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 511 581 590 598 694 2293 2608
Size: 404 107 70 9 8 96 1599 315
Cuts at: 2608 2711 2917 2923 3160 3229 3464 3500 3626
Size: 103 206 6 237 69 235 36 126
Cuts at: 3626 3677 3898 4814 5293 5432 5540 5672 5731
Size: 51 221 916 479 139 108 132 59
Cuts at: 5731 5744 5910 5984 6626 6948 7248 7361 7496
Size: 13 166 74 642 322 300 113 135
Cuts at: 7496 7531 8144 8432 8719 9065 9883 9894
Size: 35 613 288 287 346 818 11
Fragments arranged by size:
1599 916 818 642 613 479 404 346
322 315 300 288 287 237 235 221
206 166 139 135 132 126 113 108
107 103 96 74 70 69 59 51
36 35 13 11 9 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1447 1502 3541 3806 4663 6359 6571 7141
Size: 1447 55 2039 265 857 1696 212 570
Cuts at: 7141 8236 9197 9894
Size: 1095 961 697
Fragments arranged by size:
2039 1696 1447 1095 961 857 697 570
265 212 55
EcoO109I rG'GnC_Cy
Cuts at: 0 53 707 714 1865 1945 1946 2869 2870
Size: 53 654 7 1151 80 1 923 1
Cuts at: 2870 3054 3087 3088 3403 3660 3981 4710 4899
Size: 184 33 1 315 257 321 729 189
Cuts at: 4899 5121 5292 5376 5612 5813 5917 6088 6562
Size: 222 171 84 236 201 104 171 474
Cuts at: 6562 6563 6916 6950 7003 7004 7286 7639 8251
Size: 1 353 34 53 1 282 353 612
Cuts at: 8251 8716 9049 9331 9332 9826 9894
Size: 465 333 282 1 494 68
Fragments arranged by size:
1151 923 729 654 612 494 474 465
353 353 333 321 315 282 282 257
236 222 201 189 184 171 171 104
84 80 68 53 53 34 33 7
1 1 1 1 1 1
EcoRV GAT'ATC
Cuts at: 0 8763 9894
Size: 8763 1131
FspI TGC'GCA
Cuts at: 0 5734 9894
Size: 5734 4160
HaeII r_GCGC'y
Cuts at: 0 1547 4069 4161 4327 4333 4363 4456 4483
Size: 1547 2522 92 166 6 30 93 27
Cuts at: 4483 4490 4801 4939 5687 9894
Size: 7 311 138 748 4207
Fragments arranged by size:
4207 2522 1547 748 311 166 138 93
92 30 27 7 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1327 1359 1561 1593 2128 2160 2197 2229
Size: 1327 32 202 32 535 32 37 32
Cuts at: 2229 2315 2347 2509 2541 2749 2781 2905 2937
Size: 86 32 162 32 208 32 124 32
Cuts at: 2937 3013 3045 3382 3414 3513 3545 4135 4167
Size: 76 32 337 32 99 32 590 32
Cuts at: 4167 4858 4890 5164 5196 5247 5279 5378 5410
Size: 691 32 274 32 51 32 99 32
Cuts at: 5410 6086 6118 6586 6618 6895 6927 6974 7006
Size: 676 32 468 32 277 32 47 32
Cuts at: 7006 7195 7227 7948 7980 7995 8027 9894
Size: 189 32 721 32 15 32 1867
Fragments arranged by size:
1867 1327 721 691 676 590 535 468
337 277 274 208 202 189 162 124
99 99 86 76 51 47 37 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 15
HincII GTy'rAC
Cuts at: 0 1395 2225 2775 4926 5195 7272 9894
Size: 1395 830 550 2151 269 2077 2622
Fragments arranged by size:
2622 2151 2077 1395 830 550 269
HindIII A'AGCT_T
Cuts at: 0 1953 5076 8337 9894
Size: 1953 3123 3261 1557
Fragments arranged by size:
3261 3123 1953 1557
HpaI GTT'AAC
Cuts at: 0 2775 5195 9894
Size: 2775 2420 4699
Fragments arranged by size:
4699 2775 2420
KasI G'GCGC_C
Cuts at: 0 1543 4935 5683 9894
Size: 1543 3392 748 4211
Fragments arranged by size:
4211 3392 1543 748
KpnI G_GTAC'C
Cuts at: 0 457 3679 9894
Size: 457 3222 6215
Fragments arranged by size:
6215 3222 457
MfeI C'AATT_G
Cuts at: 0 8078 9894
Size: 8078 1816
MluI A'CGCG_T
Cuts at: 0 4650 4734 9894
Size: 4650 84 5160
Fragments arranged by size:
5160 4650 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1598 2561 4361 4799 6147 7830 8216 9543
Size: 1598 963 1800 438 1348 1683 386 1327
Cuts at: 9543 9894
Size: 351
Fragments arranged by size:
1800 1683 1598 1348 1327 963 438 386
351
MscI TGG'CCA
Cuts at: 0 277 1648 2245 5663 5950 6118 6745 8486
Size: 277 1371 597 3418 287 168 627 1741
Cuts at: 8486 9160 9529 9894
Size: 674 369 365
Fragments arranged by size:
3418 1741 1371 674 627 597 369 365
287 277 168
MslI CAynn'nnrTG
Cuts at: 0 1165 1202 1904 2107 2422 4105 5827 5845
Size: 1165 37 702 203 315 1683 1722 18
Cuts at: 5845 6034 6154 7300 7460 8140 8170 9089 9619
Size: 189 120 1146 160 680 30 919 530
Cuts at: 9619 9894
Size: 275
Fragments arranged by size:
1722 1683 1165 1146 919 702 680 530
315 275 203 189 160 120 37 30
18
MspA1I CmG'CkG
Cuts at: 0 169 257 333 672 972 1635 1975 2107
Size: 169 88 76 339 300 663 340 132
Cuts at: 2107 2271 3438 3918 3988 4024 4078 4320 4687
Size: 164 1167 480 70 36 54 242 367
Cuts at: 4687 5466 5740 5806 7038 7044 7413 7549 7596
Size: 779 274 66 1232 6 369 136 47
Cuts at: 7596 7723 8636 9670 9742 9894
Size: 127 913 1034 72 152
Fragments arranged by size:
1232 1167 1034 913 779 663 480 369
367 340 339 300 274 242 169 164
152 136 132 127 88 76 72 70
66 54 47 36 6
NaeI GCC'GGC
Cuts at: 0 4451 6644 9456 9894
Size: 4451 2193 2812 438
Fragments arranged by size:
4451 2812 2193 438
NarI GG'CG_CC
Cuts at: 0 1544 4936 5684 9894
Size: 1544 3392 748 4210
Fragments arranged by size:
4210 3392 1544 748
NcoI C'CATG_G
Cuts at: 0 2000 2148 9894
Size: 2000 148 7746
Fragments arranged by size:
7746 2000 148
NgoMIV G'CCGG_C
Cuts at: 0 4449 6642 9454 9894
Size: 4449 2193 2812 440
Fragments arranged by size:
4449 2812 2193 440
NheI G'CTAG_C
Cuts at: 0 6211 9894
Size: 6211 3683
NspI r_CATG'y
Cuts at: 0 1054 3595 4297 5203 7870 8188 9894
Size: 1054 2541 702 906 2667 318 1706
Fragments arranged by size:
2667 2541 1706 1054 906 702 318
PasI CC'CwG_GG
Cuts at: 0 531 2725 3358 6247 6269 6839 9822 9894
Size: 531 2194 633 2889 22 570 2983 72
Fragments arranged by size:
2983 2889 2194 633 570 531 72 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 786 987 1183 2172 2895 3151 3504 3921
Size: 786 201 196 989 723 256 353 417
Cuts at: 3921 5251 6025 6528 7992 8493 9043 9412 9525
Size: 1330 774 503 1464 501 550 369 113
Cuts at: 9525 9894
Size: 369
Fragments arranged by size:
1464 1330 989 786 774 723 550 503
501 417 369 369 353 256 201 196
113
PfoI T'CCnGG_A
Cuts at: 0 3693 6701 8087 8731 9894
Size: 3693 3008 1386 644 1163
Fragments arranged by size:
3693 3008 1386 1163 644
PmlI CAC'GTG
Cuts at: 0 4698 6649 7407 8502 9894
Size: 4698 1951 758 1095 1392
Fragments arranged by size:
4698 1951 1392 1095 758
PpuMI rG'GwC_Cy
Cuts at: 0 1865 3054 3403 3981 4710 4899 5292 5376
Size: 1865 1189 349 578 729 189 393 84
Cuts at: 5376 5612 6916 7286 8251 9049 9826 9894
Size: 236 1304 370 965 798 777 68
Fragments arranged by size:
1865 1304 1189 965 798 777 729 578
393 370 349 236 189 84 68
PshAI GACnn'nnGTC
Cuts at: 0 1863 9894
Size: 1863 8031
PspOMI G'GGCC_C
Cuts at: 0 707 1945 2869 3087 4681 5120 5812 6562
Size: 707 1238 924 218 1594 439 692 750
Cuts at: 6562 6949 7003 8885 9331 9894
Size: 387 54 1882 446 563
Fragments arranged by size:
1882 1594 1238 924 750 707 692 563
446 439 387 218 54
PstI C_TGCA'G
Cuts at: 0 357 452 944 1796 3638 3719 3828 5610
Size: 357 95 492 852 1842 81 109 1782
Cuts at: 5610 5627 5745 6176 6434 6575 7043 7418 7722
Size: 17 118 431 258 141 468 375 304
Cuts at: 7722 8960 9117 9675 9894
Size: 1238 157 558 219
Fragments arranged by size:
1842 1782 1238 852 558 492 468 431
375 357 304 258 219 157 141 118
109 95 81 17
PvuII CAG'CTG
Cuts at: 0 672 1635 1975 2107 2271 3438 3918 4024
Size: 672 963 340 132 164 1167 480 106
Cuts at: 4024 5466 5740 5806 7038 7044 7413 7596 7723
Size: 1442 274 66 1232 6 369 183 127
Cuts at: 7723 9670 9742 9894
Size: 1947 72 152
Fragments arranged by size:
1947 1442 1232 1167 963 672 480 369
340 274 183 164 152 132 127 106
72 66 6
SacI G_AGCT'C
Cuts at: 0 6848 7966 9470 9894
Size: 6848 1118 1504 424
Fragments arranged by size:
6848 1504 1118 424
SacII CC_GC'GG
Cuts at: 0 4079 4688 9894
Size: 4079 609 5206
Fragments arranged by size:
5206 4079 609
SanDI GG'GwC_CC
Cuts at: 0 3981 4710 5376 5612 7286 9826 9894
Size: 3981 729 666 236 1674 2540 68
Fragments arranged by size:
3981 2540 1674 729 666 236 68
SapI GCTCTTCn'nnn_
Cuts at: 0 37 9894
Size: 37 9857
SbfI CC_TGCA'GG
Cuts at: 0 452 1796 3638 5610 9894
Size: 452 1344 1842 1972 4284
Fragments arranged by size:
4284 1972 1842 1344 452
SexAI A'CCwGG_T
Cuts at: 0 2451 7142 9262 9894
Size: 2451 4691 2120 632
Fragments arranged by size:
4691 2451 2120 632
SfcI C'TryA_G
Cuts at: 0 353 448 667 940 1792 2302 2377 2627
Size: 353 95 219 273 852 510 75 250
Cuts at: 2627 3423 3634 3715 3824 5606 5623 5629 5741
Size: 796 211 81 109 1782 17 6 112
Cuts at: 5741 6077 6172 6309 6430 6571 6621 7039 7414
Size: 336 95 137 121 141 50 418 375
Cuts at: 7414 7517 7557 7718 8591 8956 9113 9318 9671
Size: 103 40 161 873 365 157 205 353
Cuts at: 9671 9894
Size: 223
Fragments arranged by size:
1782 873 852 796 510 418 375 365
353 353 336 273 250 223 219 211
205 161 157 141 137 121 112 109
103 95 95 81 75 50 40 17
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1829 2251 2868 7901 9166 9894
Size: 1829 422 617 5033 1265 728
Fragments arranged by size:
5033 1829 1265 728 617 422
SfoI GGC'GCC
Cuts at: 0 1545 4937 5685 9894
Size: 1545 3392 748 4209
Fragments arranged by size:
4209 3392 1545 748
SmaI CCC'GGG
Cuts at: 0 2911 4708 5482 6208 7645 9679 9894
Size: 2911 1797 774 726 1437 2034 215
Fragments arranged by size:
2911 2034 1797 1437 774 726 215
SmlI C'TyrA_G
Cuts at: 0 1317 1950 1993 4785 7156 9556 9894
Size: 1317 633 43 2792 2371 2400 338
Fragments arranged by size:
2792 2400 2371 1317 633 338 43
SphI G_CATG'C
Cuts at: 0 4297 8188 9894
Size: 4297 3891 1706
Fragments arranged by size:
4297 3891 1706
SrfI GCCC'GGGC
Cuts at: 0 9679 9894
Size: 9679 215
StuI AGG'CCT
Cuts at: 0 679 1991 2862 3457 7876 9894
Size: 679 1312 871 595 4419 2018
Fragments arranged by size:
4419 2018 1312 871 679 595
StyI C'CwwG_G
Cuts at: 0 1503 1847 2000 2148 2201 2240 3885 5747
Size: 1503 344 153 148 53 39 1645 1862
Cuts at: 5747 6554 7316 8831 8871 9071 9894
Size: 807 762 1515 40 200 823
Fragments arranged by size:
1862 1645 1515 1503 823 807 762 344
200 153 148 53 40 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 656 1261 1567 1786 1840 2376 3143 3153
Size: 656 605 306 219 54 536 767 10
Cuts at: 3153 3407 4075 4520 5886 8344 8782 9399 9644
Size: 254 668 445 1366 2458 438 617 245
Cuts at: 9644 9894
Size: 250
Fragments arranged by size:
2458 1366 767 668 656 617 605 536
445 438 306 254 250 245 219 54
10
TatI w'GTAC_w
Cuts at: 0 230 3200 3758 4364 4958 6358 7700 7773
Size: 230 2970 558 606 594 1400 1342 73
Cuts at: 7773 8028 9894
Size: 255 1866
Fragments arranged by size:
2970 1866 1400 1342 606 594 558 255
230 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 204 236 1546 1578 3689 3721 3813 3845
Size: 204 32 1310 32 2111 32 92 32
Cuts at: 3845 5861 5893 6006 6038 7231 7263 8435 8467
Size: 2016 32 113 32 1193 32 1172 32
Cuts at: 8467 8828 8860 9894
Size: 361 32 1034
Fragments arranged by size:
2111 2016 1310 1193 1172 1034 361 204
113 92 32 32 32 32 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1255 9894
Size: 1255 8639
XbaI T'CTAG_A
Cuts at: 0 7677 9894
Size: 7677 2217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1510 1692 2146 2173 2322 3079 3152 3240
Size: 1510 182 454 27 149 757 73 88
Cuts at: 3240 3475 6646 6699 9354 9894
Size: 235 3171 53 2655 540
Fragments arranged by size:
3171 2655 1510 757 540 454 235 182
149 88 73 53 27
XmaI C'CCGG_G
Cuts at: 0 2909 4706 5480 6206 7643 9677 9894
Size: 2909 1797 774 726 1437 2034 217
Fragments arranged by size:
2909 2034 1797 1437 774 726 217
ZraI GAC'GTC
Cuts at: 0 3415 4923 9894
Size: 3415 1508 4971
Fragments arranged by size:
4971 3415 1508
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApaLI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SanDI SapI SbfI SexAI SfcI
SfiI SfoI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AloI ApoI AscI AseI
AsiSI BciVI BfrBI BsiWI BstEII BstZ17I ClaI DraI
DrdI EcoRI FalI FseI FspAI NdeI NotI NruI
NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SalI ScaI SgfI SgrAI SnaBI SpeI
SspI SwaI XhoI XmnI