Restriction map for
pFN21AA1454
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII BciVI BfrI BglI
BsiWI BsmBI BspDI BspLU11I BsrFI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI Eco81I EcoO65I EcoRV
FseI FspI KpnI KspI MluI
NaeI NgoMIV NotI NruI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XhoI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 222 GTCTAC GT^[AC][GT]AC
AccII 1681 CGCG CG^CG
AccIII 1733 TCCGGA T^CCGGA
AhdI 4056 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 14 AGCGCT AGC^GCT
AspEI 4056 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2147 CCCGGG C^[CT]CG[GA]G
AvrII 2802 CCTAGG C^CTAGG
BamHI 3495 GGATCC G^GATCC
BbeI 1505 GGCGCC GGCGC^C
BbrPI 2000 CACGTG CAC^GTG
BbvCI 4144 CCTCAGC CCTCAGC(-5/-2)
BcgI 1470 CGATGTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3935 TGATCA T^GATCA
BglII 2727 AGATCT A^GATCT
BlnI 2802 CCTAGG C^CTAGG
BsaBI 3376 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 793 GAGACC GGTCTC(1/5)
BsaWI 1733 TCCGGA [AT]^CCGG[AT]
BseAI 1733 TCCGGA T^CCGGA
BsiEI 3867 CGGCCG CG[GA][CT]^CG
BsmI 3733 GCATTC GAATGC(1/-1)
BsoBI 2147 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3436 TGTACA T^GTACA
BspEI 1733 TCCGGA T^CCGGA
BsrGI 3436 TGTACA T^GTACA
BssSI 1493 CTCGTG CACGAG(-5/-1)
BstUI 1681 CGCG CG^CG
BstXI 3632 CCATCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 3296 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 3266 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3867 CGGCCG C^GGCCG
Eam1105I 4056 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3867 CGGCCG C^GGCCG
Eco47III 14 AGCGCT AGC^GCT
Eco52I 3867 CGGCCG C^GGCCG
EcoNI 3795 CCTTCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3296 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1576 ATGCAT ATGCA^T
FauI 718 CCCGC CCCGC(4/6)
FbaI 3935 TGATCA T^GATCA
HpaI 1344 GTTAAC GTT^AAC
KasI 1505 GGCGCC G^GCGCC
MamI 3376 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1733 TCCGGA T^CCGGA
MvnI 1681 CGCG CG^CG
NarI 1505 GGCGCC GG^CGCC
NdeI 3550 CATATG CA^TATG
NheI 1278 GCTAGC G^CTAGC
NsiI 1576 ATGCAT ATGCA^T
PmaCI 2000 CACGTG CAC^GTG
PmlI 2000 CACGTG CAC^GTG
PpuMI 3296 AGGACCT [GA]G^G[AT]CC[CT]
SexAI 3848 ACCAGGT A^CC[AT]GGT
SfoI 1505 GGCGCC GGC^GCC
SmaI 2147 CCCGGG CCC^GGG
SphI 3408 GCATGC GCATG^C
SspBI 3436 TGTACA T^GTACA
XcmI 3749 CCAGCTCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2147 CCCGGG C^CCGGG
XmaI 2147 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 39 enzymes) #
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AcyI 533 GACGCC G[GA]^CG[CT]C
1505 GGCGCC
Asp700I 1183 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2251 GAAAGTATTC
AspHI 293 GAGCTC G[AT]GC[AT]^C
2030 GAGCTC
BanII 293 GAGCTC G[GA]GC[CT]^C
2030 GAGCTC
BbsI 471 GAAGAC GAAGAC(2/6)
1979 GAAGAC
BlpI 2302 GCTGAGC GC^T[ACGT]AGC
2307 GCTAAGC
BmyI 293 GAGCTC G[AGT]GC[ACT]^C
2030 GAGCTC
Bpu1102I 2302 GCTGAGC GC^T[ACGT]AGC
2307 GCTAAGC
BpuAI 471 GAAGAC GAAGAC(2/6)
1979 GAAGAC
BsaAI 2000 CACGTG [CT]AC^GT[GA]
3310 TACGTG
BsaHI 533 GACGCC G[GA]^CG[CT]C
1505 GGCGCC
BseRI 632 CTCCTC GAGGAG(10/8)
55 GAGGAG
BsiHKAI 293 GAGCTC G[AT]GC[AT]^C
2030 GAGCTC
Bsp1286I 293 GAGCTC G[AGT]GC[ACT]^C
2030 GAGCTC
BspMI 1545 ACCTGC ACCTGC(4/8)
2986 ACCTGC
BsrBI 2044 GAGCGG CCGCTC(-3/-3)
2587 GAGCGG
BstBI 1091 TTCGAA TT^CGAA
2258 TTCGAA
BstYI 2727 AGATCT [GA]^GATC[CT]
3495 GGATCC
CelII 2302 GCTGAGC GC^T[ACGT]AGC
2307 GCTAAGC
CfoI 15 GCGC GCG^C
1506 GCGC
HaeII 14 AGCGCT [GA]GCGC^[CT]
1505 GGCGCC
HhaI 15 GCGC GCG^C
1506 GCGC
Hin1I 533 GACGCC G[GA]^CG[CT]C
1505 GGCGCC
HinP1I 15 GCGC G^CGC
1506 GCGC
MfeI 1613 CAATTG C^AATTG
1829 CAATTG
MflI 2727 AGATCT [GA]^GATC[CT]
3495 GGATCC
MspA1I 2192 CAGCTG C[AC]G^C[GT]G
3512 CAGCTG
MunI 1613 CAATTG C^AATTG
1829 CAATTG
NspV 1091 TTCGAA TT^CGAA
2258 TTCGAA
PflMI 1457 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1558 CCAGGGCATGG
PvuII 2192 CAGCTG CAG^CTG
3512 CAGCTG
SacI 293 GAGCTC GAGCT^C
2030 GAGCTC
ScaI 193 AGTACT AGT^ACT
3942 AGTACT
SfuI 1091 TTCGAA TT^CGAA
2258 TTCGAA
SmlI 2239 CTCAAG C^T[CT][GA]AG
4123 CTTGAG
Van91I 1457 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1558 CCAGGGCATGG
XbaI 1290 TCTAGA T^CTAGA
2034 TCTAGA
XhoII 2727 AGATCT [GA]^GATC[CT]
3495 GGATCC
XmnI 1183 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2251 GAAAGTATTC
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BanI BbvI
BcnI BfaI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BspHI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I