(Circular) (Six-base) MAPSORT of: pFN21AA1454.seq Check: 3710 from: 1 to: 8902 pFN21AA1454 8902 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5000 5000 Size: 8902 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6712 278 Size: 53 83 186 936 5176 2468 Fragments arranged by size: 5176 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2228 6245 1937 Size: 291 4017 4594 Fragments arranged by size: 4594 4017 291 Acc65I G'GTAC_C Cuts at: 6223 6223 Size: 8902 AclI AA'CG_TT Cuts at: 1563 7029 7402 1563 Size: 5466 373 3063 Fragments arranged by size: 5466 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2444 2694 2808 3219 3357 3390 3692 4392 Size: 812 250 114 411 138 33 302 700 Cuts at: 4392 4627 4700 5510 6965 8349 8617 1632 Size: 235 73 810 1455 1384 268 1917 Fragments arranged by size: 1917 1455 1384 812 810 700 411 302 268 250 235 138 114 73 33 AfeI AGC'GCT Cuts at: 2021 2021 Size: 8902 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8705 AflIII A'CryG_T Cuts at: 1203 7801 8778 1203 Size: 6598 977 1327 Fragments arranged by size: 6598 1327 977 AgeI A'CCGG_T Cuts at: 7733 7733 Size: 8902 AhdI GACnn_n'nnGTC Cuts at: 6066 7631 6066 Size: 1565 7337 AleI CACnn'nnGTG Cuts at: 1166 3006 4925 1166 Size: 1840 1919 5143 Fragments arranged by size: 5143 1919 1840 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8902 AlwNI CAG_nnn'CTG Cuts at: 6645 8217 6645 Size: 1572 7330 ApaLI G'TGCA_C Cuts at: 6959 8115 6959 Size: 1156 7746 ApoI r'AATT_y Cuts at: 1493 2605 3067 3191 3247 3573 4236 4681 4944 Size: 1112 462 124 56 326 663 445 263 Cuts at: 4944 6211 6437 6589 8698 1493 Size: 1267 226 152 2109 1697 Fragments arranged by size: 2109 1697 1267 1112 663 462 445 326 263 226 152 124 56 AseI AT'TA_AT Cuts at: 160 1032 7456 160 Size: 872 6424 1606 Fragments arranged by size: 6424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8902 AvaI C'yCGr_G Cuts at: 1870 1943 4152 6227 1870 Size: 73 2209 2075 4545 Fragments arranged by size: 4545 2209 2075 73 AvrII C'CTAG_G Cuts at: 4807 4807 Size: 8902 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5057 5090 5057 Size: 33 8869 BamHI G'GATC_C Cuts at: 5500 6232 6599 5500 Size: 732 367 7803 Fragments arranged by size: 7803 732 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2274 3510 5582 6223 7678 Size: 325 217 627 487 1236 2072 641 1455 Cuts at: 7678 618 Size: 1842 Fragments arranged by size: 2072 1842 1455 1236 641 627 487 325 217 BanII G_rGCy'C Cuts at: 1391 2302 4039 6221 1391 Size: 911 1737 2182 4072 Fragments arranged by size: 4072 2182 1737 911 BbeI G_GCGC'C Cuts at: 3514 3514 Size: 8902 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2483 3991 928 Size: 971 584 1508 5839 Fragments arranged by size: 5839 1508 971 584 BbvCI CC'TCA_GC Cuts at: 6150 6150 Size: 8902 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3464 3498 7093 7127 1903 Size: 34 1527 34 3595 34 3678 Fragments arranged by size: 3678 3595 1527 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6794 8004 6794 Size: 1210 7692 BclI T'GATC_A Cuts at: 1548 5940 1548 Size: 4392 4510 BfrBI ATG'CAT Cuts at: 3583 3583 Size: 8902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7513 136 Size: 107 122 71 631 605 5841 1525 Fragments arranged by size: 5841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4732 8897 4732 Size: 4165 4737 BlpI GC'TnA_GC Cuts at: 4308 4313 6319 4308 Size: 5 2006 6891 Fragments arranged by size: 6891 2006 5 Bme1580I G_kGCm'C Cuts at: 1790 6963 8119 1790 Size: 5173 1156 2573 Fragments arranged by size: 5173 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2317 2722 3028 Size: 416 53 83 399 24 900 405 306 Cuts at: 3028 5369 6633 7591 442 Size: 2341 1264 958 1753 Fragments arranged by size: 2341 1753 1264 958 900 416 405 399 306 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3287 1056 Size: 2231 6671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2256 3546 4548 5565 6026 6048 7562 1239 Size: 1017 1290 1002 1017 461 22 1514 2579 Fragments arranged by size: 2579 1514 1290 1017 1017 1002 461 22 Bpu10I CC'TnA_GC Cuts at: 3671 3864 4650 6150 3671 Size: 193 786 1500 6423 Fragments arranged by size: 6423 1500 786 193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4229 6149 7027 7892 8190 4229 Size: 1920 878 865 298 4941 Fragments arranged by size: 4941 1920 878 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2792 7565 882 Size: 617 1293 4773 2219 Fragments arranged by size: 4773 2219 1293 617 BsaAI yAC'GTr Cuts at: 493 1204 4007 5317 6693 493 Size: 711 2803 1310 1376 2702 Fragments arranged by size: 2803 2702 1376 1310 711 BsaBI GATnn'nnATC Cuts at: 5385 8896 5385 Size: 3511 5391 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2539 3511 6709 7091 Size: 53 83 186 936 1006 972 3198 382 Cuts at: 7091 7726 275 Size: 635 1451 Fragments arranged by size: 3198 1451 1006 972 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3738 7335 7733 8007 8154 8565 3738 Size: 3597 398 274 147 411 4075 Fragments arranged by size: 4075 3597 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8677 Fragments arranged by size: 8677 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2628 1186 Size: 889 553 7460 Fragments arranged by size: 7460 889 553 BseYI C'CCAG_C Cuts at: 4915 5597 5764 8105 8601 4915 Size: 682 167 2341 496 5216 Fragments arranged by size: 5216 2341 682 496 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2771 3842 4040 5885 1163 Size: 573 1035 1071 198 1845 4180 Fragments arranged by size: 4180 1845 1071 1035 573 198 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5875 6352 7113 8141 1540 Size: 275 186 3874 477 761 1028 2301 Fragments arranged by size: 3874 2301 1028 761 477 275 186 BsiHKAI G_wGCw'C Cuts at: 2302 4039 6963 7048 8119 2302 Size: 1737 2924 85 1071 3085 Fragments arranged by size: 3085 2924 1737 1071 85 BsmI GAATG_Cn' Cuts at: 5738 6413 6506 5738 Size: 675 93 8134 Fragments arranged by size: 8134 675 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2302 4039 6221 6963 7048 8119 1391 Size: 399 512 1737 2182 742 85 1071 2174 Fragments arranged by size: 2182 2174 1737 1071 742 512 399 85 BspEI T'CCGG_A Cuts at: 3738 8565 3738 Size: 4827 4075 BspHI T'CATG_A Cuts at: 2032 3916 5803 6791 8521 2032 Size: 1884 1887 988 1730 2413 Fragments arranged by size: 2413 1887 1884 1730 988 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3559 5000 6257 844 Size: 2715 1441 1257 3489 Fragments arranged by size: 3489 2715 1441 1257 BsrBI CCG'CTC Cuts at: 1394 1823 4051 4594 6789 1394 Size: 429 2228 543 2195 3507 Fragments arranged by size: 3507 2228 2195 543 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3318 4399 4972 5442 7397 7571 65 Size: 1183 2070 1081 573 470 1955 174 1396 Fragments arranged by size: 2070 1955 1396 1183 1081 573 470 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7546 7733 1700 Size: 110 5736 187 2869 Fragments arranged by size: 5736 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5441 96 Size: 5345 3557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8902 BssSI C'ACGA_G Cuts at: 3498 6962 7974 3498 Size: 3464 1012 4426 Fragments arranged by size: 4426 3464 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8902 BstBI TT'CG_AA Cuts at: 3097 4264 7706 3097 Size: 1167 3442 4293 Fragments arranged by size: 4293 3442 1167 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8902 BstXI CCAn_nnnn'nTGG Cuts at: 5644 5644 Size: 8902 BstYI r'GATC_y Cuts at: 1972 4732 5500 6232 6599 6984 7001 8453 8539 Size: 2760 768 732 367 385 17 1452 86 Cuts at: 8539 8551 8897 1972 Size: 12 346 1977 Fragments arranged by size: 2760 1977 1452 768 732 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3252 3462 5578 5933 513 Size: 552 939 1248 210 2116 355 3482 Fragments arranged by size: 3482 2116 1248 939 552 355 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8163 Fragments arranged by size: 8163 683 56 BtsI GCAGTG_nn' Cuts at: 2211 2847 3439 4324 4385 5023 5601 5770 6418 Size: 636 592 885 61 638 578 169 648 Cuts at: 6418 7212 7232 2211 Size: 794 20 3881 Fragments arranged by size: 3881 885 794 648 638 636 592 578 169 61 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8867 DraI TTT'AAA Cuts at: 6206 6206 Size: 8902 DraIII CAC_nnn'GTG Cuts at: 5276 5276 Size: 8902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7909 809 Size: 507 6593 1802 Fragments arranged by size: 6593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5397 5646 5751 Size: 54 1425 50 275 18 3567 249 105 Cuts at: 5751 5872 6033 6349 7238 8867 8 Size: 121 161 316 889 1629 43 Fragments arranged by size: 3567 1629 1425 889 316 275 249 161 121 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5872 6349 1537 Size: 275 4060 477 4090 Fragments arranged by size: 4090 4060 477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3273 3684 3966 4076 4215 4411 4584 5529 Size: 1920 411 282 110 139 196 173 945 Cuts at: 5529 5579 5691 6832 1353 Size: 50 112 1141 3423 Fragments arranged by size: 3423 1920 1141 945 411 282 196 173 139 112 110 50 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7489 7863 8009 387 Size: 1064 138 5900 374 146 1280 Fragments arranged by size: 5900 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2300 4037 2300 Size: 1737 7165 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 2444 2694 2808 3219 3357 3390 Size: 393 624 188 250 114 411 138 33 Cuts at: 3390 3546 3692 4392 4548 4627 4700 5510 5565 Size: 156 146 700 156 79 73 810 55 Cuts at: 5565 6026 6048 6965 7562 8349 8617 1239 Size: 461 22 917 597 787 268 1524 Fragments arranged by size: 1524 917 810 787 700 624 597 461 411 393 268 250 188 156 156 146 138 114 79 73 55 33 22 EcoNI CCTnn'n_nnAGG Cuts at: 5804 5804 Size: 8902 EcoO109I rG'GnC_Cy Cuts at: 5302 5302 Size: 8902 EcoRI G'AATT_C Cuts at: 3067 3573 4944 6211 6589 3067 Size: 506 1371 1267 378 5380 Fragments arranged by size: 5380 1371 1267 506 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4730 5062 6143 4730 Size: 332 1081 7489 Fragments arranged by size: 7489 1081 332 FspI TGC'GCA Cuts at: 7408 7408 Size: 8902 HaeII r_GCGC'y Cuts at: 1678 2023 3514 8049 1678 Size: 345 1491 4535 2531 Fragments arranged by size: 4535 2531 1491 345 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2470 2502 2539 2571 3911 Size: 32 135 32 898 32 37 32 1340 Cuts at: 3911 3943 4751 4783 5185 5217 5506 5538 5714 Size: 32 808 32 402 32 289 32 176 Cuts at: 5714 5746 6052 6084 7543 7575 7617 7649 1373 Size: 32 306 32 1459 32 42 32 2626 Fragments arranged by size: 2626 1459 1340 898 808 402 306 289 176 135 42 37 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3351 5076 5839 6246 6492 669 Size: 988 281 1413 1725 763 407 246 3079 Fragments arranged by size: 3079 1725 1413 988 763 407 281 246 HindIII A'AGCT_T Cuts at: 748 2599 3704 4572 4712 748 Size: 1851 1105 868 140 4938 Fragments arranged by size: 4938 1851 1105 868 140 HpaI GTT'AAC Cuts at: 3351 6492 3351 Size: 3141 5761 KasI G'GCGC_C Cuts at: 3510 3510 Size: 8902 KpnI G_GTAC'C Cuts at: 6227 6227 Size: 8902 MfeI C'AATT_G Cuts at: 3618 3834 6501 3618 Size: 216 2667 6019 Fragments arranged by size: 6019 2667 216 MluI A'CGCG_T Cuts at: 8778 8778 Size: 8902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2932 8016 8200 1305 Size: 1627 5084 184 2007 Fragments arranged by size: 5084 2007 1627 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5399 5648 5753 6035 10 Size: 54 1425 343 3567 249 105 282 2877 Fragments arranged by size: 3567 2877 1425 343 282 249 105 54 MslI CAynn'nnrTG Cuts at: 518 1166 2509 3006 3582 4925 5642 6860 7219 Size: 648 1343 497 576 1343 717 1218 359 Cuts at: 7219 7378 518 Size: 159 2042 Fragments arranged by size: 2042 1343 1343 1218 717 648 576 497 359 159 MspA1I CmG'CkG Cuts at: 1606 4199 5519 6318 6995 8143 8388 1606 Size: 2593 1320 799 677 1148 245 2120 Fragments arranged by size: 2593 2120 1320 1148 799 677 245 NarI GG'CG_CC Cuts at: 3511 3511 Size: 8902 NcoI C'CATG_G Cuts at: 513 1065 2004 3252 3462 5578 513 Size: 552 939 1248 210 2116 3837 Fragments arranged by size: 3837 2116 1248 939 552 210 NdeI CA'TA_TG Cuts at: 387 5556 387 Size: 5169 3733 NheI G'CTAG_C Cuts at: 1052 3283 1052 Size: 2231 6671 NotI GC'GGCC_GC Cuts at: 6349 6349 Size: 8902 NsiI A_TGCA'T Cuts at: 3585 3585 Size: 8902 NspI r_CATG'y Cuts at: 1132 1229 3583 4331 5417 5435 6260 7805 1132 Size: 97 2354 748 1086 18 825 1545 2229 Fragments arranged by size: 2354 2229 1545 1086 825 748 97 18 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8902 PciI A'CATG_T Cuts at: 7801 7801 Size: 8902 PflMI CCAn_nnn'nTGG Cuts at: 1108 3468 3569 1108 Size: 2360 101 6441 Fragments arranged by size: 6441 2360 101 PfoI T'CCnGG_A Cuts at: 2533 2533 Size: 8902 PmeI GTTT'AAAC Cuts at: 6206 6206 Size: 8902 PmlI CAC'GTG Cuts at: 4007 4007 Size: 8902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6971 7003 8484 8516 6971 Size: 32 1481 32 7357 Fragments arranged by size: 7357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5302 5302 Size: 8902 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8902 PsiI TTA'TAA Cuts at: 3887 4704 6472 3887 Size: 817 1768 6317 Fragments arranged by size: 6317 1768 817 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2953 2985 2953 Size: 32 8870 PstI C_TGCA'G Cuts at: 830 2206 5018 5412 5777 5895 6254 830 Size: 1376 2812 394 365 118 359 3478 Fragments arranged by size: 3478 2812 1376 394 365 359 118 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8902 PvuII CAG'CTG Cuts at: 4199 5519 4199 Size: 1320 7582 SacI G_AGCT'C Cuts at: 2302 4039 2302 Size: 1737 7165 SalI G'TCGA_C Cuts at: 1936 6244 1936 Size: 4308 4594 SapI GCTCTTCn'nnn_ Cuts at: 3273 3966 4584 5529 5691 3273 Size: 693 618 945 162 6484 Fragments arranged by size: 6484 945 693 618 162 SbfI CC_TGCA'GG Cuts at: 6254 6254 Size: 8902 ScaI AGT'ACT Cuts at: 2200 5949 7150 8862 2200 Size: 3749 1201 1712 2240 Fragments arranged by size: 3749 2240 1712 1201 SexAI A'CCwGG_T Cuts at: 5853 5853 Size: 8902 SfcI C'TryA_G Cuts at: 826 1045 2202 2973 3965 5014 5408 5773 5891 Size: 219 1157 771 992 1049 394 365 118 Cuts at: 5891 6250 7385 8066 8257 826 Size: 359 1135 681 191 1471 Fragments arranged by size: 1471 1157 1135 1049 992 771 681 394 365 359 219 191 118 SfoI GGC'GCC Cuts at: 3512 3512 Size: 8902 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8902 SmaI CCC'GGG Cuts at: 1872 4154 6229 1872 Size: 2282 2075 4545 Fragments arranged by size: 4545 2282 2075 SmlI C'TyrA_G Cuts at: 820 1017 1943 4244 6128 7006 7907 8169 820 Size: 197 926 2301 1884 878 901 262 1553 Fragments arranged by size: 2301 1884 1553 926 901 878 262 197 SnaBI TAC'GTA Cuts at: 493 6693 493 Size: 6200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8902 SphI G_CATG'C Cuts at: 1132 5417 6260 1132 Size: 4285 843 3774 Fragments arranged by size: 4285 3774 843 SspI AAT'ATT Cuts at: 5 52 6826 5 Size: 47 6774 2081 Fragments arranged by size: 6774 2081 47 StuI AGG'CCT Cuts at: 1517 2759 2818 6158 1517 Size: 1242 59 3340 4261 Fragments arranged by size: 4261 3340 1242 59 StyI C'CwwG_G Cuts at: 513 1065 2004 3252 3462 4807 5578 5613 6109 Size: 552 939 1248 210 1345 771 35 496 Cuts at: 6109 6341 513 Size: 232 3074 Fragments arranged by size: 3074 1345 1248 939 771 552 496 232 210 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5859 6929 6946 7099 7284 7729 325 Size: 1255 4279 1070 17 153 185 445 1498 Fragments arranged by size: 4279 1498 1255 1070 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2198 3047 4530 Size: 274 80 33 51 1443 221 849 1483 Cuts at: 4530 4556 4747 5441 5947 7148 8860 96 Size: 26 191 694 506 1201 1712 138 Fragments arranged by size: 1712 1483 1443 1201 849 694 506 274 221 191 138 80 51 33 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2529 2561 5593 5625 8862 8894 2529 Size: 32 3032 32 3237 32 2537 Fragments arranged by size: 3237 3032 2537 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8902 XbaI T'CTAG_A Cuts at: 3295 4039 6238 3295 Size: 744 2199 5959 Fragments arranged by size: 5959 2199 744 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5761 5761 Size: 8902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8902 XmaI C'CCGG_G Cuts at: 1870 4152 6227 1870 Size: 2282 2075 4545 Fragments arranged by size: 4545 2282 2075 XmnI GAAnn'nnTTC Cuts at: 3192 4260 7031 3192 Size: 1068 2771 5063 Fragments arranged by size: 5063 2771 1068 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6710 276 Size: 53 83 186 936 5176 2468 Fragments arranged by size: 5176 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI RsrII SacII SanDI SfiI SrfI SwaI