(Circular) (Six-base) MAPSORT of: pFN21AA1398.seq Check: 4019 from: 1 to: 9385 pFN21AA1398 9385 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5905 5905 Size: 9385 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5379 7195 278 Size: 53 83 186 936 3843 1816 2468 Fragments arranged by size: 3843 2468 1816 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2090 6728 1937 Size: 153 4638 4594 Fragments arranged by size: 4638 4594 153 Acc65I G'GTAC_C Cuts at: 6706 6706 Size: 9385 AclI AA'CG_TT Cuts at: 1563 7512 7885 1563 Size: 5949 373 3063 Fragments arranged by size: 5949 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2661 4787 5765 6344 6459 6521 6596 7448 Size: 1029 2126 978 579 115 62 75 852 Cuts at: 7448 8832 9100 1632 Size: 1384 268 1917 Fragments arranged by size: 2126 1917 1384 1029 978 852 579 268 115 75 62 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9188 AflIII A'CryG_T Cuts at: 1203 6045 8284 9261 1203 Size: 4842 2239 977 1327 Fragments arranged by size: 4842 2239 1327 977 AgeI A'CCGG_T Cuts at: 8216 8216 Size: 9385 AhdI GACnn_n'nnGTC Cuts at: 8114 8114 Size: 9385 AleI CACnn'nnGTG Cuts at: 1166 4724 1166 Size: 3558 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9385 AlwNI CAG_nnn'CTG Cuts at: 2585 4265 4780 4985 5053 7128 8700 2585 Size: 1680 515 205 68 2075 1572 3270 Fragments arranged by size: 3270 2075 1680 1572 515 205 68 ApaI G_GGCC'C Cuts at: 2800 2860 2920 2950 2980 3010 3040 3070 3100 Size: 60 60 30 30 30 30 30 30 Cuts at: 3100 3190 3250 3280 3310 3340 3370 3400 3430 Size: 90 60 30 30 30 30 30 30 Cuts at: 3430 3460 3490 3610 3640 3700 3730 3760 3790 Size: 30 30 120 30 60 30 30 30 Cuts at: 3790 3820 3880 3910 4371 6274 2800 Size: 30 60 30 461 1903 5911 Fragments arranged by size: 5911 1903 461 120 90 60 60 60 60 60 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 ApaLI G'TGCA_C Cuts at: 7442 8598 7442 Size: 1156 8229 ApoI r'AATT_y Cuts at: 1493 2476 6694 6920 7072 9181 1493 Size: 983 4218 226 152 2109 1697 Fragments arranged by size: 4218 2109 1697 983 226 152 AscI GG'CGCG_CC Cuts at: 6547 6547 Size: 9385 AseI AT'TA_AT Cuts at: 160 1032 7939 160 Size: 872 6907 1606 Fragments arranged by size: 6907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9385 AvaI C'yCGr_G Cuts at: 1870 1943 4704 5964 6710 1870 Size: 73 2761 1260 746 4545 Fragments arranged by size: 4545 2761 1260 746 73 BamHI G'GATC_C Cuts at: 6715 7082 6715 Size: 367 9018 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2533 2548 2561 3215 4306 Size: 325 217 627 746 15 13 654 1091 Cuts at: 4306 5936 6292 6674 6706 8161 618 Size: 1630 356 382 32 1455 1842 Fragments arranged by size: 1842 1630 1455 1091 746 654 627 382 356 325 217 32 15 13 BanII G_rGCy'C Cuts at: 1391 2311 2650 2800 2860 2890 2920 2950 2980 Size: 920 339 150 60 30 30 30 30 Cuts at: 2980 3010 3040 3070 3100 3130 3160 3190 3250 Size: 30 30 30 30 30 30 30 60 Cuts at: 3250 3280 3310 3340 3370 3400 3430 3460 3490 Size: 30 30 30 30 30 30 30 30 Cuts at: 3490 3610 3640 3700 3730 3760 3790 3820 3880 Size: 120 30 60 30 30 30 30 60 Cuts at: 3880 3910 3940 3970 4000 4030 4084 4301 4371 Size: 30 30 30 30 30 54 217 70 Cuts at: 4371 4632 4947 5472 5772 5955 6274 6704 1391 Size: 261 315 525 300 183 319 430 4072 Fragments arranged by size: 4072 920 525 430 339 319 315 300 261 217 183 150 120 70 60 60 60 60 54 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 BbeI G_GCGC'C Cuts at: 5940 6296 5940 Size: 356 9029 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 6332 6465 6584 928 Size: 971 4433 133 119 3729 Fragments arranged by size: 4433 3729 971 133 119 BbvCI CC'TCA_GC Cuts at: 3120 3210 3900 6131 3120 Size: 90 690 2231 6374 Fragments arranged by size: 6374 2231 690 90 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5396 5430 6188 6222 6664 6698 7576 Size: 34 3459 34 758 34 442 34 878 Cuts at: 7576 7610 1903 Size: 34 3678 Fragments arranged by size: 3678 3459 878 758 442 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7277 8487 7277 Size: 1210 8175 BclI T'GATC_A Cuts at: 1548 2257 1548 Size: 709 8676 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2885 3005 3035 Size: 107 122 71 631 605 1213 120 30 Cuts at: 3035 3065 3095 3155 3245 3275 3335 3365 3395 Size: 30 30 60 90 30 60 30 30 Cuts at: 3395 3425 3455 3485 3605 3635 3695 3725 3755 Size: 30 30 30 120 30 60 30 30 Cuts at: 3755 3785 3815 3935 4676 5174 5390 5540 5561 Size: 30 30 120 741 498 216 150 21 Cuts at: 5561 5657 7996 136 Size: 96 2339 1525 Fragments arranged by size: 2339 1525 1213 741 631 605 498 216 150 122 120 120 120 107 96 90 71 60 60 60 30 30 30 30 30 30 30 30 30 30 30 30 30 30 21 BglII A'GATC_T Cuts at: 9380 9380 Size: 9385 BlpI GC'TnA_GC Cuts at: 4946 6802 4946 Size: 1856 7529 Bme1580I G_kGCm'C Cuts at: 1790 2553 2800 2860 2920 2950 2980 3010 3040 Size: 763 247 60 60 30 30 30 30 Cuts at: 3040 3070 3100 3190 3220 3250 3280 3310 3340 Size: 30 30 90 30 30 30 30 30 Cuts at: 3340 3370 3400 3430 3460 3490 3610 3640 3700 Size: 30 30 30 30 30 120 30 60 Cuts at: 3700 3730 3760 3790 3820 3880 3910 4127 4371 Size: 30 30 30 30 60 30 217 244 Cuts at: 4371 6274 6677 7446 8602 1790 Size: 1903 403 769 1156 2573 Fragments arranged by size: 2573 1903 1156 769 763 403 247 244 217 120 90 60 60 60 60 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9385 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2330 2549 4170 Size: 416 53 83 399 24 913 219 1621 Cuts at: 4170 7116 8074 442 Size: 2946 958 1753 Fragments arranged by size: 2946 1753 1621 958 913 416 399 219 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9385 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5791 6508 6694 5791 Size: 717 186 8482 Fragments arranged by size: 8482 717 186 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4835 5267 5366 5450 5471 5939 6356 6377 Size: 3596 432 99 84 21 468 417 21 Cuts at: 6377 8045 1239 Size: 1668 2579 Fragments arranged by size: 3596 2579 1668 468 432 417 99 84 21 21 Bpu10I CC'TnA_GC Cuts at: 3120 3210 3900 4029 5471 6131 3120 Size: 90 690 129 1442 660 6374 Fragments arranged by size: 6374 1442 690 660 129 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5405 7510 8375 8673 5405 Size: 2105 865 298 6117 Fragments arranged by size: 6117 2105 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4420 5877 8048 882 Size: 617 2921 1457 2171 2219 Fragments arranged by size: 2921 2219 2171 1457 617 BsaAI yAC'GTr Cuts at: 493 1204 4762 6247 7176 493 Size: 711 3558 1485 929 2702 Fragments arranged by size: 3558 2702 1485 929 711 BsaBI GATnn'nnATC Cuts at: 2262 9379 2262 Size: 7117 2268 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5066 5376 5937 6293 Size: 53 83 186 936 3533 310 561 356 Cuts at: 6293 7192 7574 8209 275 Size: 899 382 635 1451 Fragments arranged by size: 3533 1451 936 899 635 561 382 356 310 186 83 53 BsaWI w'CCGG_w Cuts at: 4794 7818 8216 8490 8637 9048 4794 Size: 3024 398 274 147 411 5131 Fragments arranged by size: 5131 3024 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5417 5447 5771 5801 5903 Size: 30 165 30 4014 30 324 30 102 Cuts at: 5903 5933 6032 6062 1178 Size: 30 99 30 4501 Fragments arranged by size: 4501 4014 324 165 102 99 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2395 5099 5270 5764 5822 5918 5921 5924 Size: 1209 2704 171 494 58 96 3 3 Cuts at: 5924 5960 5963 6416 6500 1186 Size: 36 3 453 84 4071 Fragments arranged by size: 4071 2704 1209 494 453 171 96 84 58 36 3 3 3 BseYI C'CCAG_C Cuts at: 4455 5307 6266 6274 6298 8588 9084 4455 Size: 852 959 8 24 2290 496 4756 Fragments arranged by size: 4756 2290 959 852 496 24 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2360 4748 4793 5192 5564 1163 Size: 573 624 2388 45 399 372 4984 Fragments arranged by size: 4984 2388 624 573 399 372 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5556 6087 6393 6835 7596 8624 Size: 275 186 3555 531 306 442 761 1028 Cuts at: 8624 1540 Size: 2301 Fragments arranged by size: 3555 2301 1028 761 531 442 306 275 186 BsiHKAI G_wGCw'C Cuts at: 3850 4084 4632 4761 4947 5472 5822 5955 6045 Size: 234 548 129 186 525 350 133 90 Cuts at: 6045 7446 7531 8602 3850 Size: 1401 85 1071 4633 Fragments arranged by size: 4633 1401 1071 548 525 350 234 186 133 129 90 85 BsmI GAATG_Cn' Cuts at: 6896 6989 6896 Size: 93 9292 BsmBI CGTCTCn'nnnn_ Cuts at: 5804 5858 5804 Size: 54 9331 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2311 2553 2650 2800 2860 2890 2920 Size: 399 521 242 97 150 60 30 30 Cuts at: 2920 2950 2980 3010 3040 3070 3100 3130 3160 Size: 30 30 30 30 30 30 30 30 Cuts at: 3160 3190 3220 3250 3280 3310 3340 3370 3400 Size: 30 30 30 30 30 30 30 30 Cuts at: 3400 3430 3460 3490 3610 3640 3700 3730 3760 Size: 30 30 30 120 30 60 30 30 Cuts at: 3760 3790 3820 3850 3880 3910 3940 3970 4000 Size: 30 30 30 30 30 30 30 30 Cuts at: 4000 4030 4084 4127 4301 4371 4632 4761 4947 Size: 30 54 43 174 70 261 129 186 Cuts at: 4947 5472 5772 5822 5955 6045 6274 6677 6704 Size: 525 300 50 133 90 229 403 27 Cuts at: 6704 7446 7531 8602 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 525 521 403 399 300 261 242 229 186 174 150 133 129 120 97 90 85 70 60 60 54 50 43 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 27 BspEI T'CCGG_A Cuts at: 4794 9048 4794 Size: 4254 5131 BspHI T'CATG_A Cuts at: 2260 7274 9004 2260 Size: 5014 1730 2641 Fragments arranged by size: 5014 2641 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5038 5895 5905 6740 844 Size: 4194 857 10 835 3489 Fragments arranged by size: 4194 3489 857 835 10 BsrBI CCG'CTC Cuts at: 1394 1823 7272 1394 Size: 429 5449 3507 Fragments arranged by size: 5449 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7880 8054 65 Size: 1183 6632 174 1396 Fragments arranged by size: 6632 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6458 8029 8216 1700 Size: 110 4648 1571 187 2869 Fragments arranged by size: 4648 2869 1571 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9385 BssHII G'CGCG_C Cuts at: 1925 6547 1925 Size: 4622 4763 BssSI C'ACGA_G Cuts at: 4891 6556 7445 8457 4891 Size: 1665 889 1012 5819 Fragments arranged by size: 5819 1665 1012 889 BstAPI GCAn_nnn'nTGC Cuts at: 794 4780 5053 5250 6049 794 Size: 3986 273 197 799 4130 Fragments arranged by size: 4130 3986 799 273 197 BstBI TT'CG_AA Cuts at: 2498 8189 2498 Size: 5691 3694 BstEII G'GTnAC_C Cuts at: 1184 4529 1184 Size: 3345 6040 BstXI CCAn_nnnn'nTGG Cuts at: 6121 6121 Size: 9385 BstYI r'GATC_y Cuts at: 1972 4470 4791 5716 6715 7082 7467 7484 8936 Size: 2498 321 925 999 367 385 17 1452 Cuts at: 8936 9022 9034 9380 1972 Size: 86 12 346 1977 Fragments arranged by size: 2498 1977 1452 999 925 385 367 346 321 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 4419 5272 5535 5550 5643 5763 Size: 552 939 2415 853 263 15 93 120 Cuts at: 5763 513 Size: 4135 Fragments arranged by size: 4135 2415 939 853 552 263 120 93 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6213 487 Size: 683 56 4987 3659 Fragments arranged by size: 4987 3659 683 56 BtsI GCAGTG_nn' Cuts at: 5822 6901 7695 7715 5822 Size: 1079 794 20 7492 Fragments arranged by size: 7492 1079 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9385 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9350 DraI TTT'AAA Cuts at: 6689 6689 Size: 9385 DraIII CAC_nnn'GTG Cuts at: 5706 5706 Size: 9385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8392 809 Size: 507 7076 1802 Fragments arranged by size: 7076 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4581 4926 5199 Size: 54 1425 50 275 18 2751 345 273 Cuts at: 5199 5547 5553 6084 6390 6832 7721 9350 8 Size: 348 6 531 306 442 889 1629 43 Fragments arranged by size: 2751 1629 1425 889 531 442 348 345 306 275 273 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 5553 6084 6390 6832 1537 Size: 275 3741 531 306 442 4090 Fragments arranged by size: 4090 3741 531 442 306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2226 4557 4806 5888 5982 6233 7315 1353 Size: 873 2331 249 1082 94 251 1082 3423 Fragments arranged by size: 3423 2331 1082 1082 873 251 249 94 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2624 2804 5177 5249 5312 5408 Size: 1064 138 1035 180 2373 72 63 96 Cuts at: 5408 5876 7972 8346 8492 387 Size: 468 2096 374 146 1280 Fragments arranged by size: 2373 2096 1280 1064 1035 468 374 180 146 138 96 72 63 EcoICRI GAG'CTC Cuts at: 4082 4630 4945 5470 5953 4082 Size: 548 315 525 483 7514 Fragments arranged by size: 7514 548 525 483 315 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2661 4787 4835 5267 5366 5450 5471 Size: 393 1029 2126 48 432 99 84 21 Cuts at: 5471 5765 5939 6344 6356 6377 6459 6521 6596 Size: 294 174 405 12 21 82 62 75 Cuts at: 6596 7448 8045 8832 9100 1239 Size: 852 597 787 268 1524 Fragments arranged by size: 2126 1524 1029 852 787 597 432 405 393 294 268 174 99 84 82 75 62 48 21 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 4850 5108 5345 5591 4850 Size: 258 237 246 8644 Fragments arranged by size: 8644 258 246 237 EcoO109I rG'GnC_Cy Cuts at: 2796 2856 2857 2916 2946 2976 3006 3036 3066 Size: 60 1 59 30 30 30 30 30 Cuts at: 3066 3096 3186 3246 3276 3306 3336 3366 3396 Size: 30 90 60 30 30 30 30 30 Cuts at: 3396 3426 3456 3457 3486 3487 3516 3546 3576 Size: 30 30 1 29 1 29 30 30 Cuts at: 3576 3606 3636 3666 3696 3726 3756 3786 3816 Size: 30 30 30 30 30 30 30 30 Cuts at: 3816 3876 3906 4170 4200 4368 5050 5089 5338 Size: 60 30 264 30 168 682 39 249 Cuts at: 5338 5650 5732 6270 6271 6310 2796 Size: 312 82 538 1 39 5871 Fragments arranged by size: 5871 682 538 312 264 249 168 90 82 60 60 60 59 39 39 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 29 29 1 1 1 1 EcoRI G'AATT_C Cuts at: 2476 6694 7072 2476 Size: 4218 378 4789 Fragments arranged by size: 4789 4218 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6032 6032 Size: 9385 FspI TGC'GCA Cuts at: 5243 7891 5243 Size: 2648 6737 HaeII r_GCGC'y Cuts at: 1678 5104 5940 6096 6296 8532 1678 Size: 3426 836 156 200 2236 2531 Fragments arranged by size: 3426 2531 2236 836 200 156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2242 2274 2608 2640 4071 Size: 32 135 32 670 32 334 32 1431 Cuts at: 4071 4103 4460 4492 4783 4815 5417 5449 5771 Size: 32 357 32 291 32 602 32 322 Cuts at: 5771 5803 8026 8058 8100 8132 1373 Size: 32 2223 32 42 32 2626 Fragments arranged by size: 2626 2223 1431 670 602 357 334 322 291 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2091 6531 6729 6975 669 Size: 988 281 153 4440 198 246 3079 Fragments arranged by size: 4440 3079 988 281 246 198 153 HindIII A'AGCT_T Cuts at: 748 4931 748 Size: 4183 5202 HpaI GTT'AAC Cuts at: 6531 6975 6531 Size: 444 8941 KasI G'GCGC_C Cuts at: 5936 6292 5936 Size: 356 9029 KpnI G_GTAC'C Cuts at: 6710 6710 Size: 9385 MfeI C'AATT_G Cuts at: 6984 6984 Size: 9385 MluI A'CGCG_T Cuts at: 9261 9261 Size: 9385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2354 8499 8683 1305 Size: 1049 6145 184 2007 Fragments arranged by size: 6145 2007 1049 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4583 4928 5549 10 Size: 54 1425 343 2751 345 621 3846 Fragments arranged by size: 3846 2751 1425 621 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4724 7343 7702 7861 518 Size: 648 3558 2619 359 159 2042 Fragments arranged by size: 3558 2619 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2459 4232 4459 4558 4696 4780 4813 4837 Size: 853 1773 227 99 138 84 33 24 Cuts at: 4837 5008 5033 5059 5765 6322 6334 6376 6499 Size: 171 25 26 706 557 12 42 123 Cuts at: 6499 6595 6637 6801 7478 8626 8871 1606 Size: 96 42 164 677 1148 245 2120 Fragments arranged by size: 2120 1773 1148 853 706 677 557 245 227 171 164 138 123 99 96 84 42 42 33 26 25 24 12 NaeI GCC'GGC Cuts at: 6460 6460 Size: 9385 NarI GG'CG_CC Cuts at: 5937 6293 5937 Size: 356 9029 NcoI C'CATG_G Cuts at: 513 1065 2004 5535 5643 513 Size: 552 939 3531 108 4255 Fragments arranged by size: 4255 3531 939 552 108 NdeI CA'TA_TG Cuts at: 387 2116 387 Size: 1729 7656 NgoMIV G'CCGG_C Cuts at: 6458 6458 Size: 9385 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9385 NotI GC'GGCC_GC Cuts at: 6084 6832 6084 Size: 748 8637 NspI r_CATG'y Cuts at: 1132 1229 4741 5878 6423 6743 8288 1132 Size: 97 3512 1137 545 320 1545 2229 Fragments arranged by size: 3512 2229 1545 1137 545 320 97 PasI CC'CwG_GG Cuts at: 1348 2866 1348 Size: 1518 7867 PciI A'CATG_T Cuts at: 8284 8284 Size: 9385 PflMI CCAn_nnn'nTGG Cuts at: 1108 2585 1108 Size: 1477 7908 PfoI T'CCnGG_A Cuts at: 5283 5514 6354 5283 Size: 231 840 8314 Fragments arranged by size: 8314 840 231 PmeI GTTT'AAAC Cuts at: 6689 6689 Size: 9385 PmlI CAC'GTG Cuts at: 4762 4762 Size: 9385 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5311 5343 7454 7486 8967 8999 5311 Size: 32 2111 32 1481 32 5697 Fragments arranged by size: 5697 2111 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3516 3546 3576 3666 4170 4200 5050 6310 3516 Size: 30 30 90 504 30 850 1260 6591 Fragments arranged by size: 6591 1260 850 504 90 30 30 30 PshAI GACnn'nnGTC Cuts at: 1613 4412 1613 Size: 2799 6586 PsiI TTA'TAA Cuts at: 6955 6955 Size: 9385 PspOMI G'GGCC_C Cuts at: 2796 2856 2916 2946 2976 3006 3036 3066 3096 Size: 60 60 30 30 30 30 30 30 Cuts at: 3096 3186 3246 3276 3306 3336 3366 3396 3426 Size: 90 60 30 30 30 30 30 30 Cuts at: 3426 3456 3486 3606 3636 3696 3726 3756 3786 Size: 30 30 120 30 60 30 30 30 Cuts at: 3786 3816 3876 3906 4367 6270 2796 Size: 30 60 30 461 1903 5911 Fragments arranged by size: 5911 1903 461 120 90 60 60 60 60 60 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 PstI C_TGCA'G Cuts at: 830 4785 4851 5058 5064 5097 5715 5829 5892 Size: 3955 66 207 6 33 618 114 63 Cuts at: 5892 6567 6630 6737 830 Size: 675 63 107 3478 Fragments arranged by size: 3955 3478 675 618 207 114 107 66 63 63 33 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9385 PvuII CAG'CTG Cuts at: 2459 4232 4558 4696 4780 4813 4837 5033 5059 Size: 1773 326 138 84 33 24 196 26 Cuts at: 5059 6322 6334 6499 6595 6637 2459 Size: 1263 12 165 96 42 5207 Fragments arranged by size: 5207 1773 1263 326 196 165 138 96 84 42 33 26 24 12 SacI G_AGCT'C Cuts at: 4084 4632 4947 5472 5955 4084 Size: 548 315 525 483 7514 Fragments arranged by size: 7514 548 525 483 315 SacII CC_GC'GG Cuts at: 5766 5766 Size: 9385 SalI G'TCGA_C Cuts at: 1936 2089 6727 1936 Size: 153 4638 4594 Fragments arranged by size: 4638 4594 153 SanDI GG'GwC_CC Cuts at: 3516 3546 3576 3666 4170 4200 3516 Size: 30 30 90 504 30 8701 Fragments arranged by size: 8701 504 90 30 30 30 SapI GCTCTTCn'nnn_ Cuts at: 5888 6233 5888 Size: 345 9040 SbfI CC_TGCA'GG Cuts at: 6737 6737 Size: 9385 ScaI AGT'ACT Cuts at: 7633 9345 7633 Size: 1712 7673 SexAI A'CCwGG_T Cuts at: 6030 6030 Size: 9385 SfcI C'TryA_G Cuts at: 826 1045 2469 4781 4847 5054 5060 5093 5711 Size: 219 1424 2312 66 207 6 33 618 Cuts at: 5711 5825 5888 6563 6626 6733 7868 8549 8740 Size: 114 63 675 63 107 1135 681 191 Cuts at: 8740 826 Size: 1471 Fragments arranged by size: 2312 1471 1424 1135 681 675 618 219 207 191 114 107 66 63 63 33 6 SfiI GGCCn_nnn'nGGCC Cuts at: 3005 3035 3065 3095 3245 3275 3335 3365 3395 Size: 30 30 30 150 30 60 30 30 Cuts at: 3395 3425 3455 3485 3605 3635 3695 3725 3755 Size: 30 30 30 120 30 60 30 30 Cuts at: 3755 3785 3815 5561 3005 Size: 30 30 1746 6829 Fragments arranged by size: 6829 1746 150 120 60 60 30 30 30 30 30 30 30 30 30 30 30 30 30 30 SfoI GGC'GCC Cuts at: 5938 6294 5938 Size: 356 9029 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9385 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9385 SmaI CCC'GGG Cuts at: 1872 4706 5966 6712 1872 Size: 2834 1260 746 4545 Fragments arranged by size: 4545 2834 1260 746 SmlI C'TyrA_G Cuts at: 820 1017 1943 5420 7489 8390 8652 820 Size: 197 926 3477 2069 901 262 1553 Fragments arranged by size: 3477 2069 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7176 493 Size: 6683 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9385 SphI G_CATG'C Cuts at: 1132 4741 5878 6743 1132 Size: 3609 1137 865 3774 Fragments arranged by size: 3774 3609 1137 865 SspI AAT'ATT Cuts at: 5 52 7309 5 Size: 47 7257 2081 Fragments arranged by size: 7257 2081 47 StuI AGG'CCT Cuts at: 1517 5564 6491 1517 Size: 4047 927 4411 Fragments arranged by size: 4411 4047 927 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2412 2526 2565 2716 2746 Size: 552 939 24 384 114 39 151 30 Cuts at: 2746 2776 4060 4210 5025 5535 5598 5643 5928 Size: 30 1284 150 815 510 63 45 285 Cuts at: 5928 6114 6824 513 Size: 186 710 3074 Fragments arranged by size: 3074 1284 939 815 710 552 510 384 285 186 151 150 114 63 45 39 30 30 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2549 7412 7429 7582 7767 8212 325 Size: 1255 969 4863 17 153 185 445 1498 Fragments arranged by size: 4863 1498 1255 969 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7631 9343 96 Size: 274 80 33 51 1443 5654 1712 138 Fragments arranged by size: 5654 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5903 5935 9345 9377 5903 Size: 32 3410 32 5911 Fragments arranged by size: 5911 3410 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4255 5111 1316 Size: 2939 856 5590 Fragments arranged by size: 5590 2939 856 XbaI T'CTAG_A Cuts at: 6721 6721 Size: 9385 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4834 4897 5542 6251 4834 Size: 63 645 709 7968 Fragments arranged by size: 7968 709 645 63 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9385 XmaI C'CCGG_G Cuts at: 1870 4704 5964 6710 1870 Size: 2834 1260 746 4545 Fragments arranged by size: 4545 2834 1260 746 XmnI GAAnn'nnTTC Cuts at: 5326 7514 5326 Size: 2188 7197 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5377 7193 276 Size: 53 83 186 936 3843 1816 2468 Fragments arranged by size: 3843 2468 1816 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SrfI SwaI