Restriction map for
pFN21AA1315
(Circular) (Six-base) MAPSORT of: pFN21AA1315.seq Check: 7062 from: 1 to: 10648
pFN21AA1315 10648 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8458 278
Size: 53 83 186 936 6922 2468
Fragments arranged by size:
6922 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3009 6239 7991 1937
Size: 1072 3230 1752 4594
Fragments arranged by size:
4594 3230 1752 1072
Acc65I G'GTAC_C
Cuts at: 7969 7969
Size: 10648
AclI AA'CG_TT
Cuts at: 1563 3893 8775 9148 1563
Size: 2330 4882 373 3063
Fragments arranged by size:
4882 3063 2330 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2214 3432 3615 4254 4436 6162 6374 6432
Size: 582 1218 183 639 182 1726 212 58
Cuts at: 6432 6888 7638 8711 10095 10363 1632
Size: 456 750 1073 1384 268 1917
Fragments arranged by size:
1917 1726 1384 1218 1073 750 639 582
456 268 212 183 182 58
AfeI AGC'GCT
Cuts at: 2134 5759 2134
Size: 3625 7023
AflII C'TTAA_G
Cuts at: 820 1017 2069 2336 2800 5324 5441 6050 820
Size: 197 1052 267 464 2524 117 609 5418
Fragments arranged by size:
5418 2524 1052 609 464 267 197 117
AflIII A'CryG_T
Cuts at: 1203 4775 4963 5124 5146 9547 10524 1203
Size: 3572 188 161 22 4401 977 1327
Fragments arranged by size:
4401 3572 1327 977 188 161 22
AgeI A'CCGG_T
Cuts at: 9479 9479
Size: 10648
AhdI GACnn_n'nnGTC
Cuts at: 9377 9377
Size: 10648
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2603 1184
Size: 1419 9229
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5732 5764 5732
Size: 32 10616
AlwNI CAG_nnn'CTG
Cuts at: 2936 3929 4136 7079 8391 9963 2936
Size: 993 207 2943 1312 1572 3621
Fragments arranged by size:
3621 2943 1572 1312 993 207
ApaLI G'TGCA_C
Cuts at: 8705 9861 8705
Size: 1156 9492
ApoI r'AATT_y
Cuts at: 1493 2305 4358 4681 4783 4864 5168 5180 5710
Size: 812 2053 323 102 81 304 12 530
Cuts at: 5710 6887 7100 7809 7957 8183 8335 10444 1493
Size: 1177 213 709 148 226 152 2109 1697
Fragments arranged by size:
2109 2053 1697 1177 812 709 530 323
304 226 213 152 148 102 81 12
AseI AT'TA_AT
Cuts at: 160 1032 4113 5189 9202 160
Size: 872 3081 1076 4013 1606
Fragments arranged by size:
4013 3081 1606 1076 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10648
AvaI C'yCGr_G
Cuts at: 1870 1943 7973 1870
Size: 73 6030 4545
Fragments arranged by size:
6030 4545 73
BamHI G'GATC_C
Cuts at: 7978 8345 7978
Size: 367 10281
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7969 9424 618
Size: 325 217 627 6182 1455 1842
Fragments arranged by size:
6182 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4023 6854 7967 1391
Size: 2632 2831 1113 4072
Fragments arranged by size:
4072 2831 2632 1113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3506 3783 3972 5610 6168 7287 928
Size: 971 1607 277 189 1638 558 1119 4289
Fragments arranged by size:
4289 1638 1607 1119 971 558 277 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8839 8873 1903
Size: 34 6902 34 3678
Fragments arranged by size:
6902 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4186 8540 9750 4186
Size: 4354 1210 5084
Fragments arranged by size:
5084 4354 1210
BclI T'GATC_A
Cuts at: 1548 2536 3109 6619 7129 1548
Size: 988 573 3510 510 5067
Fragments arranged by size:
5067 3510 988 573 510
BfrBI ATG'CAT
Cuts at: 3367 4009 6467 6592 7365 3367
Size: 642 2458 125 773 6650
Fragments arranged by size:
6650 2458 773 642 125
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9259 136
Size: 107 122 71 631 605 7587 1525
Fragments arranged by size:
7587 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2491 2504 3155 5021 6319 7057 10643 2491
Size: 13 651 1866 1298 738 3586 2496
Fragments arranged by size:
3586 2496 1866 1298 738 651 13
BlpI GC'TnA_GC
Cuts at: 8065 8065
Size: 10648
Bme1580I G_kGCm'C
Cuts at: 1790 8709 9865 1790
Size: 6919 1156 2573
Fragments arranged by size:
6919 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4919 6644 1318
Size: 3601 1725 5322
Fragments arranged by size:
5322 3601 1725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7401 8379 9337
Size: 416 53 83 399 24 5984 978 958
Cuts at: 9337 442
Size: 1753
Fragments arranged by size:
5984 1753 978 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10648
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 10648
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3006 3169 4433 5659 6009 6515 6543 7167
Size: 1767 163 1264 1226 350 506 28 624
Cuts at: 7167 9308 1239
Size: 2141 2579
Fragments arranged by size:
2579 2141 1767 1264 1226 624 506 350
163 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4620 6473 8773 9638 9936 4620
Size: 1853 2300 865 298 5332
Fragments arranged by size:
5332 2300 1853 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2972 4549 4591 7900 9311 882
Size: 617 1473 1577 42 3309 1411 2219
Fragments arranged by size:
3309 2219 1577 1473 1411 617 42
BsaAI yAC'GTr
Cuts at: 493 1204 7054 8439 493
Size: 711 5850 1385 2702
Fragments arranged by size:
5850 2702 1385 711
BsaBI GATnn'nnATC
Cuts at: 2571 3304 3944 6366 7527 7881 10642 2571
Size: 733 640 2422 1161 354 2761 2577
Fragments arranged by size:
2761 2577 2422 1161 733 640 354
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8455 8837 9472 275
Size: 53 83 186 936 6922 382 635 1451
Fragments arranged by size:
6922 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 9081 9479 9753 9900 10311 9081
Size: 398 274 147 411 9418
Fragments arranged by size:
9418 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3688 3718 7387 7417 1178
Size: 30 165 30 2285 30 3669 30 4409
Fragments arranged by size:
4409 3669 2285 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2055 1186
Size: 869 9779
BseYI C'CCAG_C
Cuts at: 2105 5838 9851 10347 2105
Size: 3733 4013 496 2406
Fragments arranged by size:
4013 3733 2406 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2653 1163
Size: 573 917 9158
Fragments arranged by size:
9158 917 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8098 8859 9887 1540
Size: 275 186 6097 761 1028 2301
Fragments arranged by size:
6097 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5060 6430 6854 7171 8709 8794 9865 5060
Size: 1370 424 317 1538 85 1071 5843
Fragments arranged by size:
5843 1538 1370 1071 424 317 85
BsmI GAATG_Cn'
Cuts at: 5527 8159 8252 5527
Size: 2632 93 7923
Fragments arranged by size:
7923 2632 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4023 5060 6430 6854 7171 7967 8709
Size: 399 2233 1037 1370 424 317 796 742
Cuts at: 8709 8794 9865 1391
Size: 85 1071 2174
Fragments arranged by size:
2233 2174 1370 1071 1037 796 742 424
399 317 85
BspEI T'CCGG_A
Cuts at: 10311 10311
Size: 10648
BspHI T'CATG_A
Cuts at: 4510 6838 8537 10267 4510
Size: 2328 1699 1730 4891
Fragments arranged by size:
4891 2328 1730 1699
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 8003 844
Size: 7159 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 8535 1394
Size: 429 6712 3507
Fragments arranged by size:
6712 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3627 6188 9143 9317 65
Size: 1183 2379 2561 2955 174 1396
Fragments arranged by size:
2955 2561 2379 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6193 9292 9479 1700
Size: 110 4383 3099 187 2869
Fragments arranged by size:
4383 3099 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2569 4760 6074 96
Size: 2473 2191 1314 4670
Fragments arranged by size:
4670 2473 2191 1314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10648
BssSI C'ACGA_G
Cuts at: 8708 9720 8708
Size: 1012 9636
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6294 7721 794
Size: 5500 1427 3721
Fragments arranged by size:
5500 3721 1427
BstBI TT'CG_AA
Cuts at: 2443 2912 9452 2443
Size: 469 6540 3639
Fragments arranged by size:
6540 3639 469
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10648
BstXI CCAn_nnnn'nTGG
Cuts at: 7093 7093
Size: 10648
BstYI r'GATC_y
Cuts at: 1972 2184 2491 2504 3155 5021 6319 7057 7069
Size: 212 307 13 651 1866 1298 738 12
Cuts at: 7069 7669 7978 8345 8730 8747 10199 10285 10297
Size: 600 309 367 385 17 1452 86 12
Cuts at: 10297 10643 1972
Size: 346 1977
Fragments arranged by size:
1977 1866 1452 1298 738 651 600 385
367 346 309 307 212 86 17 13
12 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3873 513
Size: 552 939 1869 7288
Fragments arranged by size:
7288 1869 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6330 487
Size: 683 56 5104 4805
Fragments arranged by size:
5104 4805 683 56
BtsI GCAGTG_nn'
Cuts at: 2585 2665 3930 5989 8164 8958 8978 2585
Size: 80 1265 2059 2175 794 20 4255
Fragments arranged by size:
4255 2175 2059 1265 794 80 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10648
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3751 3786 529
Size: 35 3187 35 7391
Fragments arranged by size:
7391 3187 35 35
DraI TTT'AAA
Cuts at: 2287 2920 3390 3537 5947 6381 6781 7158 7359
Size: 633 470 147 2410 434 400 377 201
Cuts at: 7359 7846 7952 2287
Size: 487 106 4983
Fragments arranged by size:
4983 2410 633 487 470 434 400 377
201 147 106
DraIII CAC_nnn'GTG
Cuts at: 4919 4919
Size: 10648
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9655 809
Size: 507 8339 1802
Fragments arranged by size:
8339 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 8095 8984 10613
Size: 54 1425 50 275 18 6265 889 1629
Cuts at: 10613 8
Size: 43
Fragments arranged by size:
6265 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 8095 1537
Size: 275 6283 4090
Fragments arranged by size:
6283 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2175 2731 2968 3225 3232 3921 4011 5107
Size: 822 556 237 257 7 689 90 1096
Cuts at: 5107 5473 5748 6078 6453 6667 8578 1353
Size: 366 275 330 375 214 1911 3423
Fragments arranged by size:
3423 1911 1096 822 689 556 375 366
330 275 257 237 214 90 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9235 9609 9755 387
Size: 1064 138 7646 374 146 1280
Fragments arranged by size:
7646 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6852 6852
Size: 10648
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 3006 3169 3432 3615 4254 4433
Size: 393 582 792 163 263 183 639 179
Cuts at: 4433 4436 5659 6009 6162 6374 6432 6515 6543
Size: 3 1223 350 153 212 58 83 28
Cuts at: 6543 6888 7167 7638 8711 9308 10095 10363 1239
Size: 345 279 471 1073 597 787 268 1524
Fragments arranged by size:
1524 1223 1073 792 787 639 597 582
471 393 350 345 279 268 263 212
183 179 163 153 83 58 28 3
EcoO109I rG'GnC_Cy
Cuts at: 3661 7651 3661
Size: 3990 6658
EcoRI G'AATT_C
Cuts at: 4783 7100 7957 8335 4783
Size: 2317 857 378 7096
Fragments arranged by size:
7096 2317 857 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2652 5467 5516 6546 2652
Size: 2815 49 1030 6754
Fragments arranged by size:
6754 2815 1030 49
FspI TGC'GCA
Cuts at: 9154 9154
Size: 10648
HaeII r_GCGC'y
Cuts at: 1678 2136 5761 9795 1678
Size: 458 3625 4034 2531
Fragments arranged by size:
4034 3625 2531 458
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 2483 2515 3773
Size: 32 135 32 500 32 379 32 1258
Cuts at: 3773 3805 4385 4417 4508 4540 6125 6157 6380
Size: 32 580 32 91 32 1585 32 223
Cuts at: 6380 6412 6452 6484 7424 7436 7456 7468 7654
Size: 32 40 32 940 12 20 12 186
Cuts at: 7654 7661 7686 7693 7761 7793 9289 9321 9363
Size: 7 25 7 68 32 1496 32 42
Cuts at: 9363 9395 1373
Size: 32 2626
Fragments arranged by size:
2626 1585 1496 1258 940 580 500 379
223 186 135 91 68 42 40 32
32 32 32 32 32 32 32 32
32 32 32 32 25 20 12 12
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2255 3891 3997 5989 6834 7992
Size: 988 281 317 1636 106 1992 845 1158
Cuts at: 7992 8238 669
Size: 246 3079
Fragments arranged by size:
3079 1992 1636 1158 988 845 317 281
246 106
HindIII A'AGCT_T
Cuts at: 748 5438 5470 748
Size: 4690 32 5926
Fragments arranged by size:
5926 4690 32
HpaI GTT'AAC
Cuts at: 5989 8238 5989
Size: 2249 8399
KpnI G_GTAC'C
Cuts at: 7973 7973
Size: 10648
MfeI C'AATT_G
Cuts at: 4305 8247 4305
Size: 3942 6706
MluI A'CGCG_T
Cuts at: 10524 10524
Size: 10648
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 5589 6192 6831 7854 9762 9946 1305
Size: 786 3498 603 639 1023 1908 184 2007
Fragments arranged by size:
3498 2007 1908 1023 786 639 603 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 8826
Fragments arranged by size:
8826 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2517 4515 4665 5123 6204 6435 6924
Size: 648 1351 1998 150 458 1081 231 489
Cuts at: 6924 8606 8965 9124 518
Size: 1682 359 159 2042
Fragments arranged by size:
2042 1998 1682 1351 1081 648 489 458
359 231 159 150
MspA1I CmG'CkG
Cuts at: 1606 3857 4886 6037 8064 8741 9889 10134 1606
Size: 2251 1029 1151 2027 677 1148 245 2120
Fragments arranged by size:
2251 2120 2027 1151 1148 1029 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3873 513
Size: 552 939 1869 7288
Fragments arranged by size:
7288 1869 939 552
NdeI CA'TA_TG
Cuts at: 387 3817 387
Size: 3430 7218
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10648
NotI GC'GGCC_GC
Cuts at: 8095 8095
Size: 10648
NsiI A_TGCA'T
Cuts at: 3369 4011 6469 6594 7367 3369
Size: 642 2458 125 773 6650
Fragments arranged by size:
6650 2458 773 642 125
NspI r_CATG'y
Cuts at: 1132 1229 4779 4967 5128 5150 5846 6596 8006
Size: 97 3550 188 161 22 696 750 1410
Cuts at: 8006 9551 1132
Size: 1545 2229
Fragments arranged by size:
3550 2229 1545 1410 750 696 188 161
97 22
PacI TTA_AT'TAA
Cuts at: 5244 5244
Size: 10648
PasI CC'CwG_GG
Cuts at: 1348 7694 1348
Size: 6346 4302
PciI A'CATG_T
Cuts at: 4775 4963 5124 5146 9547 4775
Size: 188 161 22 4401 5876
Fragments arranged by size:
5876 4401 188 161 22
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4480 4919 1108
Size: 3372 439 6837
Fragments arranged by size:
6837 3372 439
PfoI T'CCnGG_A
Cuts at: 6536 6536
Size: 10648
PmeI GTTT'AAAC
Cuts at: 7952 7952
Size: 10648
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8717 8749 10230 10262 8717
Size: 32 1481 32 9103
Fragments arranged by size:
9103 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3661 3661
Size: 10648
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10648
PsiI TTA'TAA
Cuts at: 5619 8218 5619
Size: 2599 8049
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4596 4628 5358 5390 4596
Size: 32 730 32 9854
Fragments arranged by size:
9854 730 32 32
PstI C_TGCA'G
Cuts at: 830 8000 830
Size: 7170 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10648
PvuII CAG'CTG
Cuts at: 3857 4886 6037 3857
Size: 1029 1151 8468
Fragments arranged by size:
8468 1151 1029
SacI G_AGCT'C
Cuts at: 6854 6854
Size: 10648
SalI G'TCGA_C
Cuts at: 1936 7990 1936
Size: 6054 4594
SapI GCTCTTCn'nnn_
Cuts at: 3921 4011 5748 6078 3921
Size: 90 1737 330 8491
Fragments arranged by size:
8491 1737 330 90
SbfI CC_TGCA'GG
Cuts at: 8000 8000
Size: 10648
ScaI AGT'ACT
Cuts at: 2635 2806 4930 8896 10608 2635
Size: 171 2124 3966 1712 2675
Fragments arranged by size:
3966 2675 2124 1712 171
SfcI C'TryA_G
Cuts at: 826 1045 3483 4602 7203 7996 9131 9812 10003
Size: 219 2438 1119 2601 793 1135 681 191
Cuts at: 10003 826
Size: 1471
Fragments arranged by size:
2601 2438 1471 1135 1119 793 681 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10648
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10648
SmaI CCC'GGG
Cuts at: 1872 7975 1872
Size: 6103 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 2336 2800 4635 5324 5441
Size: 197 926 126 267 464 1835 689 117
Cuts at: 5441 6050 6452 8752 9653 9915 820
Size: 609 402 2300 901 262 1553
Fragments arranged by size:
2300 1835 1553 926 901 689 609 464
402 267 262 197 126 117
SnaBI TAC'GTA
Cuts at: 493 7054 8439 493
Size: 6561 1385 2702
Fragments arranged by size:
6561 2702 1385
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10648
SphI G_CATG'C
Cuts at: 1132 5846 6596 8006 1132
Size: 4714 750 1410 3774
Fragments arranged by size:
4714 3774 1410 750
SspI AAT'ATT
Cuts at: 5 52 2347 5943 7176 8572 5
Size: 47 2295 3596 1233 1396 2081
Fragments arranged by size:
3596 2295 2081 1396 1233 47
StuI AGG'CCT
Cuts at: 1517 7354 1517
Size: 5837 4811
StyI C'CwwG_G
Cuts at: 513 1065 2004 2828 3873 5763 8087 513
Size: 552 939 824 1045 1890 2324 3074
Fragments arranged by size:
3074 2324 1890 1045 939 824 552
SwaI ATTT'AAAT
Cuts at: 7158 7158
Size: 10648
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5924 8675 8692 8845 9030 9475 325
Size: 1255 4344 2751 17 153 185 445 1498
Fragments arranged by size:
4344 2751 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2569 2633 2804
Size: 274 80 33 51 1443 592 64 171
Cuts at: 2804 4760 4928 5770 5993 6074 8894 10606 96
Size: 1956 168 842 223 81 2820 1712 138
Fragments arranged by size:
2820 1956 1712 1443 842 592 274 223
171 168 138 81 80 64 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7281 7313 7387 7419 10608 10640 7281
Size: 32 74 32 3189 32 7289
Fragments arranged by size:
7289 3189 74 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10648
XbaI T'CTAG_A
Cuts at: 2501 3226 4438 7984 2501
Size: 725 1212 3546 5165
Fragments arranged by size:
5165 3546 1212 725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10648
XmaI C'CCGG_G
Cuts at: 1870 7973 1870
Size: 6103 4545
XmnI GAAnn'nnTTC
Cuts at: 7038 7516 8777 7038
Size: 478 1261 8909
Fragments arranged by size:
8909 1261 478
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8456 276
Size: 53 83 186 936 6922 2468
Fragments arranged by size:
6922 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PmlI PspOMI PspXI
RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI