Restriction map for
pFN21AA1313
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
AflII AgeI AhdI Alw44I AocI
ApaLI AscI AspEI AviII AvrII
BamHI BcgI BciVI BfrI BlnI
BsiWI Bsp1407I BspDI BspLU11I BsrGI
Bst1107I BstZ17I Bsu36I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI FseI FspI HpaI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI PacI PinAI
PmeI PvuI PvuII RsrII SalI
SbfI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AatII 1663 GACGTC GACGT^C
Acc65I 2223 GGTACC G^GTACC
AccIII 2129 TCCGGA T^CCGGA
AclI 2257 AACGTT AA^CGTT
AcsI 1588 GAATTC [GA]^AATT[CT]
Aor51HI 454 AGCGCT AGC^GCT
ApoI 1588 GAATTC [GA]^AATT[CT]
AseI 67 ATTAAT AT^TAAT
AsnI 67 ATTAAT AT^TAAT
Asp700I 2716 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2223 GGTACC G^GTACC
BbeI 1405 GGCGCC GGCGC^C
BbrPI 928 CACGTG CAC^GTG
BbvCI 2938 GCTGAGG CCTCAGC(-5/-2)
BglI 118 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 410 AGATCT A^GATCT
BlpI 3190 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3190 GCTGAGC GC^T[ACGT]AGC
BsaBI 2701 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1447 GGTCTC GGTCTC(1/5)
BsaWI 2129 TCCGGA [AT]^CCGG[AT]
BseAI 2129 TCCGGA T^CCGGA
BsmBI 595 GAGACG CGTCTC(1/5)
BsmI 2737 GAATGC GAATGC(1/-1)
BspEI 2129 TCCGGA T^CCGGA
BspHI 2703 TCATGA T^CATGA
BsrBI 2337 CCGCTC CCGCTC(-3/-3)
BssHII 99 GCGCGC G^CGCGC
BstAPI 2313 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1585 TTCGAA TT^CGAA
CelII 3190 GCTGAGC GC^T[ACGT]AGC
DraIII 208 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1495 GACATGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 454 AGCGCT AGC^GCT
EcoRI 1588 GAATTC G^AATTC
EcoRV 3292 GATATC GAT^ATC
EcoT22I 3223 ATGCAT ATGCA^T
HindIII 477 AAGCTT A^AGCTT
KasI 1405 GGCGCC G^GCGCC
KpnI 2223 GGTACC GGTAC^C
KspI 1833 CCGCGG CCGC^GG
MamI 2701 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2129 TCCGGA T^CCGGA
MslI 2667 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1405 GGCGCC GG^CGCC
NcoI 2681 CCATGG C^CATGG
NsiI 3223 ATGCAT ATGCA^T
NspV 1585 TTCGAA TT^CGAA
PaeR7I 307 CTCGAG C^TCGAG
PflMI 38 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 928 CACGTG CAC^GTG
PmlI 928 CACGTG CAC^GTG
PpuMI 359 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 67 ATTAAT AT^TAAT
Psp1406I 2257 AACGTT AA^CGTT
RcaI 2703 TCATGA T^CATGA
SacII 1833 CCGCGG CCGC^GG
SapI 2270 GCTCTTC GCTCTTC(1/4)
SfoI 1405 GGCGCC GGC^GCC
SfuI 1585 TTCGAA TT^CGAA
Van91I 38 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 307 CTCGAG C^TCGAG
XmnI 2716 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 820 GTCTAC GT^[AC][GT]AC
1723 GTCTAC
AcyI 1405 GGCGCC G[GA]^CG[CT]C
1663 GACGTC
AflIII 929 ACGTGT A^C[GA][CT]GT
1146 ACGTGT
AlwNI 2314 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
2763 CAGGTGCTG
ApaI 964 GGGCCC GGGCC^C
2428 GGGCCC
AspI 597 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1852 GACCAGGTC
BbsI 1777 GAAGAC GAAGAC(2/6)
1504 GTCTTC
BclI 2700 TGATCA T^GATCA
2706 TGATCA
BfaI 211 CTAG C^TAG
631 CTAG
BpuAI 1777 GAAGAC GAAGAC(2/6)
1504 GTCTTC
BsaHI 1405 GGCGCC G[GA]^CG[CT]C
1663 GACGTC
BsiEI 846 CGACCG CG[GA][CT]^CG
1830 CGACCG
BspMI 1040 ACCTGC ACCTGC(4/8)
1485 GCAGGT
BsrFI 18 GCCGGT [GA]^CCGG[CT]
1029 GCCGGT
BssSI 1567 CACGAG CACGAG(-5/-1)
568 CTCGTG
Cfr10I 18 GCCGGT [GA]^CCGG[CT]
1029 GCCGGT
FbaI 2700 TGATCA T^GATCA
2706 TGATCA
Hin1I 1405 GGCGCC G[GA]^CG[CT]C
1663 GACGTC
MaeI 211 CTAG C^TAG
631 CTAG
NruI 1125 TCGCGA TCG^CGA
1431 TCGCGA
NspI 1496 ACATGC [GA]CATG^[CT]
2814 GCATGT
PflFI 597 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1852 GACCAGGTC
PshAI 1441 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1761 GACTGTTGTC
ScaI 77 AGTACT AGT^ACT
2156 AGTACT
SmaI 1089 CCCGGG CCC^GGG
2228 CCCGGG
StuI 1134 AGGCCT AGG^CCT
2947 AGGCCT
TfiI 620 GAATC G^A[AT]TC
2745 GATTC
Tth111I 597 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1852 GACCAGGTC
XcmI 806 CCAACGGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1208 CCACTATTCTGGTGG
XmaCI 1089 CCCGGG C^CCGGG
2228 CCCGGG
XmaI 1089 CCCGGG C^CCGGG
2228 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII