Restriction map for
pFN21AA1313
(Linear) (Six-base) MAPSORT of: KIAA1313.seq Check: 95 from: 1 to: 3303
KIAA1313.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1476 3303
Size: 1476 1827
AatII G_ACGT'C
Cuts at: 0 1667 3303
Size: 1667 1636
AccI GT'mk_AC
Cuts at: 0 821 1724 3303
Size: 821 903 1579
Fragments arranged by size:
1579 903 821
Acc65I G'GTAC_C
Cuts at: 0 2223 3303
Size: 2223 1080
AclI AA'CG_TT
Cuts at: 0 2258 3303
Size: 2258 1045
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 2590 3206 3265 3303
Size: 158 2432 616 59 38
Fragments arranged by size:
2432 616 158 59 38
AfeI AGC'GCT
Cuts at: 0 456 3303
Size: 456 2847
AflIII A'CryG_T
Cuts at: 0 929 1146 3303
Size: 929 217 2157
Fragments arranged by size:
2157 929 217
AleI CACnn'nnGTG
Cuts at: 0 2671 3303
Size: 2671 632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2121 3303
Size: 2121 1182
AlwNI CAG_nnn'CTG
Cuts at: 0 2319 2768 3303
Size: 2319 449 535
Fragments arranged by size:
2319 535 449
ApaI G_GGCC'C
Cuts at: 0 968 2432 3303
Size: 968 1464 871
Fragments arranged by size:
1464 968 871
ApoI r'AATT_y
Cuts at: 0 1588 3303
Size: 1588 1715
AseI AT'TA_AT
Cuts at: 0 68 3303
Size: 68 3235
AvaI C'yCGr_G
Cuts at: 0 307 1089 2228 3303
Size: 307 782 1139 1075
Fragments arranged by size:
1139 1075 782 307
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2633 2666 3303
Size: 2633 33 637
Fragments arranged by size:
2633 637 33
BanI G'GyrC_C
Cuts at: 0 658 802 1405 2223 3303
Size: 658 144 603 818 1080
Fragments arranged by size:
1080 818 658 603 144
BanII G_rGCy'C
Cuts at: 0 289 503 863 968 1900 2020 2059 2432
Size: 289 214 360 105 932 120 39 373
Cuts at: 2432 2520 3079 3197 3303
Size: 88 559 118 106
Fragments arranged by size:
932 559 373 360 289 214 120 118
106 105 88 39
BbeI G_GCGC'C
Cuts at: 0 1409 3303
Size: 1409 1894
BbsI GAAGACnn'nnnn_
Cuts at: 0 1497 1784 3303
Size: 1497 287 1519
Fragments arranged by size:
1519 1497 287
BbvCI CC'TCA_GC
Cuts at: 0 2939 3303
Size: 2939 364
BclI T'GATC_A
Cuts at: 0 2700 2706 3303
Size: 2700 6 597
Fragments arranged by size:
2700 597 6
BfrBI ATG'CAT
Cuts at: 0 3225 3303
Size: 3225 78
BglI GCCn_nnn'nGGC
Cuts at: 0 124 3303
Size: 124 3179
BglII A'GATC_T
Cuts at: 0 410 3303
Size: 410 2893
BlpI GC'TnA_GC
Cuts at: 0 3191 3303
Size: 3191 112
Bme1580I G_kGCm'C
Cuts at: 0 661 805 929 968 1178 1292 2432 3303
Size: 661 144 124 39 210 114 1140 871
Fragments arranged by size:
1140 871 661 210 144 124 114 39
BmgBI CAC'GTC
Cuts at: 0 945 1147 3303
Size: 945 202 2156
Fragments arranged by size:
2156 945 202
BmrI ACTGGGnnnn_n'
Cuts at: 0 374 3303
Size: 374 2929
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1221 3303
Size: 1221 2082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 547 3160 3303
Size: 427 120 2613 143
Fragments arranged by size:
2613 427 143 120
Bpu10I CC'TnA_GC
Cuts at: 0 953 2810 2939 3303
Size: 953 1857 129 364
Fragments arranged by size:
1857 953 364 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 3055 3303
Size: 58 2997 248
Fragments arranged by size:
2997 248 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 1453 3303
Size: 1453 1850
BsaAI yAC'GTr
Cuts at: 0 930 1098 2505 3303
Size: 930 168 1407 798
Fragments arranged by size:
1407 930 798 168
BsaBI GATnn'nnATC
Cuts at: 0 2705 3303
Size: 2705 598
BsaHI Gr'CG_yC
Cuts at: 0 1406 1664 3303
Size: 1406 258 1639
Fragments arranged by size:
1639 1406 258
BsaWI w'CCGG_w
Cuts at: 0 2129 3303
Size: 2129 1174
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 2254 2274 2602 2956 3303
Size: 106 2148 20 328 354 347
Fragments arranged by size:
2148 354 347 328 106 20
BseYI C'CCAG_C
Cuts at: 0 226 1164 2432 3126 3303
Size: 226 938 1268 694 177
Fragments arranged by size:
1268 938 694 226 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 967 1171 1405 3303
Size: 508 459 204 234 1898
Fragments arranged by size:
1898 508 459 234 204
BsiEI CG_ry'CG
Cuts at: 0 849 1833 3303
Size: 849 984 1470
Fragments arranged by size:
1470 984 849
BsiHKAI G_wGCw'C
Cuts at: 0 503 863 1325 1900 2350 3079 3303
Size: 503 360 462 575 450 729 224
Fragments arranged by size:
729 575 503 462 450 360 224
BsmI GAATG_Cn'
Cuts at: 0 2743 3303
Size: 2743 560
BsmBI CGTCTCn'nnnn_
Cuts at: 0 589 3303
Size: 589 2714
Bsp1286I G_dGCh'C
Cuts at: 0 289 503 661 805 863 929 968 1178
Size: 289 214 158 144 58 66 39 210
Cuts at: 1178 1292 1325 1900 2020 2059 2350 2432 2520
Size: 114 33 575 120 39 291 82 88
Cuts at: 2520 3079 3197 3303
Size: 559 118 106
Fragments arranged by size:
575 559 291 289 214 210 158 144
120 118 114 106 88 82 66 58
39 39 33
BspEI T'CCGG_A
Cuts at: 0 2129 3303
Size: 2129 1174
BspHI T'CATG_A
Cuts at: 0 2703 3303
Size: 2703 600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1049 1476 3303
Size: 1049 427 1827
Fragments arranged by size:
1827 1049 427
BsrBI CCG'CTC
Cuts at: 0 2339 3303
Size: 2339 964
BsrDI GCAATG_nn'
Cuts at: 0 1176 2060 2724 3033 3110 3303
Size: 1176 884 664 309 77 193
Fragments arranged by size:
1176 884 664 309 193 77
BsrFI r'CCGG_y
Cuts at: 0 18 1029 3303
Size: 18 1011 2274
Fragments arranged by size:
2274 1011 18
BssHII G'CGCG_C
Cuts at: 0 99 3303
Size: 99 3204
BssSI C'ACGA_G
Cuts at: 0 568 1567 3303
Size: 568 999 1736
Fragments arranged by size:
1736 999 568
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2319 3303
Size: 2319 984
BstBI TT'CG_AA
Cuts at: 0 1586 3303
Size: 1586 1717
BstEII G'GTnAC_C
Cuts at: 0 243 681 993 3303
Size: 243 438 312 2310
Fragments arranged by size:
2310 438 312 243
BstXI CCAn_nnnn'nTGG
Cuts at: 0 327 1215 1861 3117 3303
Size: 327 888 646 1256 186
Fragments arranged by size:
1256 888 646 327 186
BstYI r'GATC_y
Cuts at: 0 410 2126 2686 3303
Size: 410 1716 560 617
Fragments arranged by size:
1716 617 560 410
BtgI C'CryG_G
Cuts at: 0 1833 1938 2681 3198 3303
Size: 1833 105 743 517 105
Fragments arranged by size:
1833 743 517 105 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2814 3303
Size: 2814 489
BtsI GCAGTG_nn'
Cuts at: 0 623 984 1159 1464 1624 1712 3303
Size: 623 361 175 305 160 88 1591
Fragments arranged by size:
1591 623 361 305 175 160 88
DraIII CAC_nnn'GTG
Cuts at: 0 213 3303
Size: 213 3090
DrdI GACnn_nn'nnGTC
Cuts at: 0 1501 3303
Size: 1501 1802
EaeI y'GGCC_r
Cuts at: 0 35 125 810 1601 1800 2751 3303
Size: 35 90 685 791 199 951 552
Fragments arranged by size:
951 791 685 552 199 90 35
EarI CTCTTCn'nnn_
Cuts at: 0 83 914 2277 3303
Size: 83 831 1363 1026
Fragments arranged by size:
1363 1026 831 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 763 1016 2206 3303
Size: 763 253 1190 1097
Fragments arranged by size:
1190 1097 763 253
EcoICRI GAG'CTC
Cuts at: 0 501 861 1898 3077 3303
Size: 501 360 1037 1179 226
Fragments arranged by size:
1179 1037 501 360 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 427 547 2590 3160 3206 3265 3303
Size: 158 269 120 2043 570 46 59 38
Fragments arranged by size:
2043 570 269 158 120 59 46 38
EcoO109I rG'GnC_Cy
Cuts at: 0 360 964 2428 2429 3303
Size: 360 604 1464 1 874
Fragments arranged by size:
1464 874 604 360 1
EcoRI G'AATT_C
Cuts at: 0 1588 3303
Size: 1588 1715
EcoRV GAT'ATC
Cuts at: 0 3294 3303
Size: 3294 9
HaeII r_GCGC'y
Cuts at: 0 458 908 1085 1409 3303
Size: 458 450 177 324 1894
Fragments arranged by size:
1894 458 450 324 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 454 486 586 618 1114 1146
Size: 301 32 121 32 100 32 496 32
Cuts at: 1146 2685 2717 3303
Size: 1539 32 586
Fragments arranged by size:
1539 586 496 301 121 100 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 843 1053 1668 2652 3003 3153 3162 3303
Size: 843 210 615 984 351 150 9 141
Fragments arranged by size:
984 843 615 351 210 150 141 9
HindIII A'AGCT_T
Cuts at: 0 477 3303
Size: 477 2826
KasI G'GCGC_C
Cuts at: 0 1405 3303
Size: 1405 1898
KpnI G_GTAC'C
Cuts at: 0 2227 3303
Size: 2227 1076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 2522 3303
Size: 218 2304 781
Fragments arranged by size:
2304 781 218
MscI TGG'CCA
Cuts at: 0 37 127 1603 2753 3303
Size: 37 90 1476 1150 550
Fragments arranged by size:
1476 1150 550 90 37
MslI CAynn'nnrTG
Cuts at: 0 2671 3303
Size: 2671 632
MspA1I CmG'CkG
Cuts at: 0 447 1835 2625 3303
Size: 447 1388 790 678
Fragments arranged by size:
1388 790 678 447
NarI GG'CG_CC
Cuts at: 0 1406 3303
Size: 1406 1897
NcoI C'CATG_G
Cuts at: 0 2681 3303
Size: 2681 622
NruI TCG'CGA
Cuts at: 0 1127 1433 3303
Size: 1127 306 1870
Fragments arranged by size:
1870 1127 306
NsiI A_TGCA'T
Cuts at: 0 3227 3303
Size: 3227 76
NspI r_CATG'y
Cuts at: 0 1500 2818 3303
Size: 1500 1318 485
Fragments arranged by size:
1500 1318 485
PasI CC'CwG_GG
Cuts at: 0 2052 2325 3303
Size: 2052 273 978
Fragments arranged by size:
2052 978 273
PflMI CCAn_nnn'nTGG
Cuts at: 0 44 3303
Size: 44 3259
PfoI T'CCnGG_A
Cuts at: 0 3014 3303
Size: 3014 289
PmlI CAC'GTG
Cuts at: 0 930 3303
Size: 930 2373
PpuMI rG'GwC_Cy
Cuts at: 0 360 3303
Size: 360 2943
PshAI GACnn'nnGTC
Cuts at: 0 1445 1765 3303
Size: 1445 320 1538
Fragments arranged by size:
1538 1445 320
PspOMI G'GGCC_C
Cuts at: 0 964 2428 3303
Size: 964 1464 875
Fragments arranged by size:
1464 964 875
PspXI vC'TCGA_Gb
Cuts at: 0 307 3303
Size: 307 2996
PstI C_TGCA'G
Cuts at: 0 1190 1301 2146 2992 3303
Size: 1190 111 845 846 311
Fragments arranged by size:
1190 846 845 311 111
SacI G_AGCT'C
Cuts at: 0 503 863 1900 3079 3303
Size: 503 360 1037 1179 224
Fragments arranged by size:
1179 1037 503 360 224
SacII CC_GC'GG
Cuts at: 0 1836 3303
Size: 1836 1467
SapI GCTCTTCn'nnn_
Cuts at: 0 2277 3303
Size: 2277 1026
ScaI AGT'ACT
Cuts at: 0 79 2158 3303
Size: 79 2079 1145
Fragments arranged by size:
2079 1145 79
SexAI A'CCwGG_T
Cuts at: 0 1373 1754 1853 3303
Size: 1373 381 99 1450
Fragments arranged by size:
1450 1373 381 99
SfcI C'TryA_G
Cuts at: 0 609 1186 1297 2142 2988 3303
Size: 609 577 111 845 846 315
Fragments arranged by size:
846 845 609 577 315 111
SfoI GGC'GCC
Cuts at: 0 1407 3303
Size: 1407 1896
SmaI CCC'GGG
Cuts at: 0 1091 2230 3303
Size: 1091 1139 1073
Fragments arranged by size:
1139 1091 1073
SmlI C'TyrA_G
Cuts at: 0 73 307 3070 3303
Size: 73 234 2763 233
Fragments arranged by size:
2763 234 233 73
StuI AGG'CCT
Cuts at: 0 1136 2949 3303
Size: 1136 1813 354
Fragments arranged by size:
1813 1136 354
StyI C'CwwG_G
Cuts at: 0 1106 1577 1604 2681 2996 3303
Size: 1106 471 27 1077 315 307
Fragments arranged by size:
1106 1077 471 315 307 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 700 1837 3303
Size: 700 1137 1466
Fragments arranged by size:
1466 1137 700
TatI w'GTAC_w
Cuts at: 0 77 2156 3303
Size: 77 2079 1147
Fragments arranged by size:
2079 1147 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 755 787 3303
Size: 755 32 2516
Fragments arranged by size:
2516 755 32
Tth111I GACn'n_nGTC
Cuts at: 0 600 1855 3303
Size: 600 1255 1448
Fragments arranged by size:
1448 1255 600
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 813 1215 3303
Size: 813 402 2088
Fragments arranged by size:
2088 813 402
XhoI C'TCGA_G
Cuts at: 0 307 3303
Size: 307 2996
XmaI C'CCGG_G
Cuts at: 0 1089 2228 3303
Size: 1089 1139 1075
Fragments arranged by size:
1139 1089 1075
XmnI GAAnn'nnTTC
Cuts at: 0 2720 3303
Size: 2720 583
ZraI GAC'GTC
Cuts at: 0 1665 3303
Size: 1665 1638
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaI ApoI AseI AvaI BaeI
BanI BanII BbeI BbsI BbvCI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI NruI NsiI NspI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI
SacI SacII SapI ScaI SexAI SfcI SfoI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI ApaLI AscI AsiSI AvrII
BamHI BcgI BciVI BmtI BsaXI BsiWI BsrGI BstZ17I
Bsu36I ClaI CspCI DraI EagI EcoNI FalI FseI
FspI FspAI HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NotI PacI PciI PmeI PpiI PsiI PsrI
PvuI PvuII RsrII SalI SanDI SbfI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI