(Circular) (Six-base) MAPSORT of: pFN21AA1286.seq Check: 3218 from: 1 to: 8320 pFN21AA1286 8320 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4140 5533 4140 Size: 1393 6927 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6130 278 Size: 53 83 186 936 4594 2468 Fragments arranged by size: 4594 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2133 2568 5663 1937 Size: 196 435 3095 4594 Fragments arranged by size: 4594 3095 435 196 Acc65I G'GTAC_C Cuts at: 4754 5641 4754 Size: 887 7433 AclI AA'CG_TT Cuts at: 1563 6447 6820 1563 Size: 4884 373 3063 Fragments arranged by size: 4884 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2211 2460 2916 3263 3671 4259 5246 5381 Size: 579 249 456 347 408 588 987 135 Cuts at: 5381 6383 7767 8035 1632 Size: 1002 1384 268 1917 Fragments arranged by size: 1917 1384 1002 987 588 579 456 408 347 268 249 135 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8123 AflIII A'CryG_T Cuts at: 1203 3056 7219 8196 1203 Size: 1853 4163 977 1327 Fragments arranged by size: 4163 1853 1327 977 AgeI A'CCGG_T Cuts at: 7151 7151 Size: 8320 AhdI GACnn_n'nnGTC Cuts at: 7049 7049 Size: 8320 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4148 5555 1184 Size: 2964 1407 3949 Fragments arranged by size: 3949 2964 1407 AlwNI CAG_nnn'CTG Cuts at: 4348 4944 5390 6063 7635 4348 Size: 596 446 673 1572 5033 Fragments arranged by size: 5033 1572 673 596 446 ApaI G_GGCC'C Cuts at: 2617 2869 4216 4294 4422 5592 2617 Size: 252 1347 78 128 1170 5345 Fragments arranged by size: 5345 1347 1170 252 128 78 ApaLI G'TGCA_C Cuts at: 3060 6377 7533 3060 Size: 3317 1156 3847 Fragments arranged by size: 3847 3317 1156 ApoI r'AATT_y Cuts at: 1493 3887 5629 5855 6007 8116 1493 Size: 2394 1742 226 152 2109 1697 Fragments arranged by size: 2394 2109 1742 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4590 6874 160 Size: 872 3558 2284 1606 Fragments arranged by size: 3558 2284 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8320 AvaI C'yCGr_G Cuts at: 1870 1943 2088 4115 4136 5133 5304 5645 1870 Size: 73 145 2027 21 997 171 341 4545 Fragments arranged by size: 4545 2027 997 341 171 145 73 21 BamHI G'GATC_C Cuts at: 2889 3829 5650 6017 2889 Size: 940 1821 367 5192 Fragments arranged by size: 5192 1821 940 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2834 3119 4754 5641 7096 Size: 325 217 627 1047 285 1635 887 1455 Cuts at: 7096 618 Size: 1842 Fragments arranged by size: 1842 1635 1455 1047 887 627 325 285 217 BanII G_rGCy'C Cuts at: 1391 2617 2869 3078 3096 4216 4294 4422 4524 Size: 1226 252 209 18 1120 78 128 102 Cuts at: 4524 4572 4650 5592 5639 1391 Size: 48 78 942 47 4072 Fragments arranged by size: 4072 1226 1120 942 252 209 128 102 78 78 48 47 18 BbeI G_GCGC'C Cuts at: 2838 2838 Size: 8320 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2448 2643 3632 4161 4185 4476 5217 Size: 971 549 195 989 529 24 291 741 Cuts at: 5217 5487 928 Size: 270 3761 Fragments arranged by size: 3761 989 971 741 549 529 291 270 195 24 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6511 6545 1903 Size: 34 4574 34 3678 Fragments arranged by size: 4574 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4405 6212 7422 4405 Size: 1807 1210 5303 Fragments arranged by size: 5303 1807 1210 BclI T'GATC_A Cuts at: 1548 5283 1548 Size: 3735 4585 BfrBI ATG'CAT Cuts at: 2324 2324 Size: 8320 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3117 4417 4528 Size: 107 122 71 631 605 1445 1300 111 Cuts at: 4528 6931 136 Size: 2403 1525 Fragments arranged by size: 2403 1525 1445 1300 631 605 122 111 107 71 BglII A'GATC_T Cuts at: 3462 8315 3462 Size: 4853 3467 BlpI GC'TnA_GC Cuts at: 3603 5737 3603 Size: 2134 6186 Bme1580I G_kGCm'C Cuts at: 1790 2532 2617 2862 2869 3064 3339 4127 4216 Size: 742 85 245 7 195 275 788 89 Cuts at: 4216 4294 4422 5142 5592 6381 7537 1790 Size: 78 128 720 450 789 1156 2573 Fragments arranged by size: 2573 1156 789 788 742 720 450 275 245 195 128 89 85 78 7 BmgBI CAC'GTC Cuts at: 1318 4183 5356 1318 Size: 2865 1173 4282 Fragments arranged by size: 4282 2865 1173 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2872 4536 6051 Size: 416 53 83 399 24 1455 1664 1515 Cuts at: 6051 7009 442 Size: 958 1753 Fragments arranged by size: 1753 1664 1515 1455 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8320 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2031 3178 3743 4143 4933 5417 2031 Size: 1147 565 400 790 484 4934 Fragments arranged by size: 4934 1147 790 565 484 400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3791 3953 4271 4463 4538 4868 4875 5412 Size: 2552 162 318 192 75 330 7 537 Cuts at: 5412 6980 1239 Size: 1568 2579 Fragments arranged by size: 2579 2552 1568 537 330 318 192 162 75 7 Bpu10I CC'TnA_GC Cuts at: 5182 5573 5182 Size: 391 7929 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2057 2597 3288 3311 3883 5483 6445 7310 7608 Size: 540 691 23 572 1600 962 865 298 Cuts at: 7608 2057 Size: 2769 Fragments arranged by size: 2769 1600 962 865 691 572 540 298 23 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2087 3479 5060 5214 6983 882 Size: 617 588 1392 1581 154 1769 2219 Fragments arranged by size: 2219 1769 1581 1392 617 588 154 BsaAI yAC'GTr Cuts at: 493 1204 6111 493 Size: 711 4907 2702 Fragments arranged by size: 4907 2702 711 BsaBI GATnn'nnATC Cuts at: 8314 8314 Size: 8320 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2146 2739 2835 5096 Size: 53 83 186 936 613 593 96 2261 Cuts at: 5096 6127 6509 7144 275 Size: 1031 382 635 1451 Fragments arranged by size: 2261 1451 1031 936 635 613 593 382 186 96 83 53 BsaWI w'CCGG_w Cuts at: 6753 7151 7425 7572 7983 6753 Size: 398 274 147 411 7090 Fragments arranged by size: 7090 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3392 3422 3638 3668 4154 Size: 30 165 30 1989 30 216 30 486 Cuts at: 4154 4184 4933 4963 5228 5258 1178 Size: 30 749 30 265 30 4240 Fragments arranged by size: 4240 1989 749 486 265 216 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2492 3275 3659 3974 3983 4475 4494 1186 Size: 1306 783 384 315 9 492 19 5012 Fragments arranged by size: 5012 1306 783 492 384 315 19 9 BseYI C'CCAG_C Cuts at: 2115 2354 3071 4974 5365 7523 8019 2115 Size: 239 717 1903 391 2158 496 2416 Fragments arranged by size: 2416 2158 1903 717 496 391 239 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3027 3654 3819 4253 4518 5303 5561 Size: 573 1291 627 165 434 265 785 258 Cuts at: 5561 1163 Size: 3922 Fragments arranged by size: 3922 1291 785 627 573 434 265 258 165 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4752 5770 6531 7559 1540 Size: 275 186 2751 1018 761 1028 2301 Fragments arranged by size: 2751 2301 1028 1018 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2081 3064 4650 6381 6466 7537 2081 Size: 983 1586 1731 85 1071 2864 Fragments arranged by size: 2864 1731 1586 1071 983 85 BsmI GAATG_Cn' Cuts at: 2041 2228 3044 5831 5924 2041 Size: 187 816 2787 93 4437 Fragments arranged by size: 4437 2787 816 187 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2532 2617 2862 2869 3064 3078 Size: 399 291 451 85 245 7 195 14 Cuts at: 3078 3096 3339 4127 4216 4294 4422 4524 4572 Size: 18 243 788 89 78 128 102 48 Cuts at: 4572 4650 5142 5592 5639 6381 6466 7537 1391 Size: 78 492 450 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 788 742 492 451 450 399 291 245 243 195 128 102 89 85 85 78 78 48 47 18 14 7 BspEI T'CCGG_A Cuts at: 7983 7983 Size: 8320 BspHI T'CATG_A Cuts at: 3081 6209 7939 3081 Size: 3128 1730 3462 Fragments arranged by size: 3462 3128 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2984 3730 3822 4140 4602 5533 5675 844 Size: 2140 746 92 318 462 931 142 3489 Fragments arranged by size: 3489 2140 931 746 462 318 142 92 BsrBI CCG'CTC Cuts at: 1394 1823 3685 6207 1394 Size: 429 1862 2522 3507 Fragments arranged by size: 3507 2522 1862 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2689 6815 6989 65 Size: 1183 1441 4126 174 1396 Fragments arranged by size: 4126 1441 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2046 2571 4751 6964 7151 1700 Size: 110 236 525 2180 2213 187 2869 Fragments arranged by size: 2869 2213 2180 525 236 187 110 BsrGI T'GTAC_A Cuts at: 96 2719 96 Size: 2623 5697 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8320 BssSI C'ACGA_G Cuts at: 6380 7392 6380 Size: 1012 7308 BstAPI GCAn_nnn'nTGC Cuts at: 794 2817 4453 4696 794 Size: 2023 1636 243 4418 Fragments arranged by size: 4418 2023 1636 243 BstBI TT'CG_AA Cuts at: 7124 7124 Size: 8320 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8320 BstXI CCAn_nnnn'nTGG Cuts at: 3769 3904 4633 5617 3769 Size: 135 729 984 6472 Fragments arranged by size: 6472 984 729 135 BstYI r'GATC_y Cuts at: 1972 2889 3462 3829 5650 6017 6402 6419 7871 Size: 917 573 367 1821 367 385 17 1452 Cuts at: 7871 7957 7969 8315 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1821 1452 917 573 385 367 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2754 2754 Size: 8320 BtgI C'CryG_G Cuts at: 513 1065 3762 4864 5113 5215 5487 513 Size: 552 2697 1102 249 102 272 3346 Fragments arranged by size: 3346 2697 1102 552 272 249 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3243 5576 487 Size: 683 56 2017 2333 3231 Fragments arranged by size: 3231 2333 2017 683 56 BtsI GCAGTG_nn' Cuts at: 2614 3180 3642 4664 5092 5447 5836 6630 6650 Size: 566 462 1022 428 355 389 794 20 Cuts at: 6650 2614 Size: 4284 Fragments arranged by size: 4284 1022 794 566 462 428 389 355 20 ClaI AT'CG_AT Cuts at: 1554 2631 5289 1554 Size: 1077 2658 4585 Fragments arranged by size: 4585 2658 1077 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2812 2847 529 Size: 35 2248 35 6002 Fragments arranged by size: 6002 2248 35 35 DraI TTT'AAA Cuts at: 5624 5624 Size: 8320 DraIII CAC_nnn'GTG Cuts at: 3173 4766 3173 Size: 1593 6727 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7327 809 Size: 507 6011 1802 Fragments arranged by size: 6011 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3177 4749 4928 Size: 54 1425 50 275 18 1347 1572 179 Cuts at: 4928 5767 6656 8285 8 Size: 839 889 1629 43 Fragments arranged by size: 1629 1572 1425 1347 889 839 275 179 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4749 5767 1537 Size: 275 2937 1018 4090 Fragments arranged by size: 4090 2937 1018 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2699 3083 3264 3270 3285 3710 4799 4902 Size: 1346 384 181 6 15 425 1089 103 Cuts at: 4902 4980 5408 5420 6250 1353 Size: 78 428 12 830 3423 Fragments arranged by size: 3423 1346 1089 830 428 425 384 181 103 78 15 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3940 4034 4298 4497 6907 7281 Size: 1064 138 2351 94 264 199 2410 374 Cuts at: 7281 7427 387 Size: 146 1280 Fragments arranged by size: 2410 2351 1280 1064 374 264 199 146 138 94 EcoICRI GAG'CTC Cuts at: 4648 4648 Size: 8320 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2460 2916 3263 3671 3791 3953 Size: 393 579 249 456 347 408 120 162 Cuts at: 3953 4259 4271 4463 4538 4868 4875 5246 5381 Size: 306 12 192 75 330 7 371 135 Cuts at: 5381 5412 6383 6980 7767 8035 1239 Size: 31 971 597 787 268 1524 Fragments arranged by size: 1524 971 787 597 579 456 408 393 371 347 330 306 268 249 192 162 135 120 75 31 12 7 EcoNI CCTnn'n_nnAGG Cuts at: 3448 3484 3818 3448 Size: 36 334 7950 Fragments arranged by size: 7950 334 36 EcoO109I rG'GnC_Cy Cuts at: 2482 2613 4054 4290 4418 4419 4843 4968 5311 Size: 131 1441 236 128 1 424 125 343 Cuts at: 5311 5588 5589 2482 Size: 277 1 5213 Fragments arranged by size: 5213 1441 424 343 277 236 131 128 125 1 1 EcoRI G'AATT_C Cuts at: 3887 5629 6007 3887 Size: 1742 378 6200 Fragments arranged by size: 6200 1742 378 EcoRV GAT'ATC Cuts at: 2215 2215 Size: 8320 FspI TGC'GCA Cuts at: 3137 6826 3137 Size: 3689 4631 HaeII r_GCGC'y Cuts at: 1678 2838 3571 4275 4915 7467 1678 Size: 1160 733 704 640 2552 2531 Fragments arranged by size: 2552 2531 1160 733 704 640 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2570 2602 3392 3424 3726 Size: 32 135 32 998 32 790 32 302 Cuts at: 3726 3758 4026 4053 4058 4085 4541 4573 4713 Size: 32 268 27 5 27 456 32 140 Cuts at: 4713 4745 5275 5307 6961 6993 7035 7067 1373 Size: 32 530 32 1654 32 42 32 2626 Fragments arranged by size: 2626 1654 998 790 530 456 302 268 140 135 42 32 32 32 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 3859 5664 5910 669 Size: 988 281 1921 1805 246 3079 Fragments arranged by size: 3079 1921 1805 988 281 246 HindIII A'AGCT_T Cuts at: 748 5079 748 Size: 4331 3989 HpaI GTT'AAC Cuts at: 5910 5910 Size: 8320 KasI G'GCGC_C Cuts at: 2834 2834 Size: 8320 KpnI G_GTAC'C Cuts at: 4758 5645 4758 Size: 887 7433 MfeI C'AATT_G Cuts at: 5919 5919 Size: 8320 MluI A'CGCG_T Cuts at: 8196 8196 Size: 8320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2000 7434 7618 1305 Size: 695 5434 184 2007 Fragments arranged by size: 5434 2007 695 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6498 Fragments arranged by size: 6498 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 6278 6637 6796 518 Size: 648 5112 359 159 2042 Fragments arranged by size: 5112 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2119 2401 2775 2964 3067 3508 4040 4237 Size: 513 282 374 189 103 441 532 197 Cuts at: 4237 4436 5217 5350 5736 6413 7561 7806 1606 Size: 199 781 133 386 677 1148 245 2120 Fragments arranged by size: 2120 1148 781 677 532 513 441 386 374 282 245 199 197 189 133 103 NaeI GCC'GGC Cuts at: 2048 2048 Size: 8320 NarI GG'CG_CC Cuts at: 2835 2835 Size: 8320 NcoI C'CATG_G Cuts at: 513 1065 3762 4864 513 Size: 552 2697 1102 3969 Fragments arranged by size: 3969 2697 1102 552 NdeI CA'TA_TG Cuts at: 387 2104 387 Size: 1717 6603 NgoMIV G'CCGG_C Cuts at: 2046 2046 Size: 8320 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8320 NotI GC'GGCC_GC Cuts at: 5767 5767 Size: 8320 NsiI A_TGCA'T Cuts at: 2326 2326 Size: 8320 NspI r_CATG'y Cuts at: 1132 1229 2324 3060 5678 7223 1132 Size: 97 1095 736 2618 1545 2229 Fragments arranged by size: 2618 2229 1545 1095 736 97 PasI CC'CwG_GG Cuts at: 1348 4423 1348 Size: 3075 5245 PciI A'CATG_T Cuts at: 3056 7219 3056 Size: 4163 4157 PflMI CCAn_nnn'nTGG Cuts at: 1108 4766 4870 1108 Size: 3658 104 4558 Fragments arranged by size: 4558 3658 104 PfoI T'CCnGG_A Cuts at: 4845 4919 5145 4845 Size: 74 226 8020 Fragments arranged by size: 8020 226 74 PmeI GTTT'AAAC Cuts at: 5624 5624 Size: 8320 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5150 5182 6389 6421 7902 7934 5150 Size: 32 1207 32 1481 32 5536 Fragments arranged by size: 5536 1481 1207 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2482 4054 4843 4968 2482 Size: 1572 789 125 5834 Fragments arranged by size: 5834 1572 789 125 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8320 PsiI TTA'TAA Cuts at: 5890 5890 Size: 8320 PspOMI G'GGCC_C Cuts at: 2613 2865 4212 4290 4418 5588 2613 Size: 252 1347 78 128 1170 5345 Fragments arranged by size: 5345 1347 1170 252 128 78 PstI C_TGCA'G Cuts at: 830 2548 2787 3588 3822 4671 5672 830 Size: 1718 239 801 234 849 1001 3478 Fragments arranged by size: 3478 1718 1001 849 801 239 234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8320 PvuII CAG'CTG Cuts at: 4040 4237 4436 4040 Size: 197 199 7924 Fragments arranged by size: 7924 199 197 SacI G_AGCT'C Cuts at: 4650 4650 Size: 8320 SacII CC_GC'GG Cuts at: 5218 5218 Size: 8320 SalI G'TCGA_C Cuts at: 1936 5662 1936 Size: 3726 4594 SapI GCTCTTCn'nnn_ Cuts at: 3083 3285 4902 5408 3083 Size: 202 1617 506 5995 Fragments arranged by size: 5995 1617 506 202 SbfI CC_TGCA'GG Cuts at: 5672 5672 Size: 8320 ScaI AGT'ACT Cuts at: 6568 8280 6568 Size: 1712 6608 SexAI A'CCwGG_T Cuts at: 3169 4263 5616 3169 Size: 1094 1353 5873 Fragments arranged by size: 5873 1353 1094 SfcI C'TryA_G Cuts at: 826 1045 2065 2544 2783 3466 3584 3818 4667 Size: 219 1020 479 239 683 118 234 849 Cuts at: 4667 4823 4945 5668 6803 7484 7675 826 Size: 156 122 723 1135 681 191 1471 Fragments arranged by size: 1471 1135 1020 849 723 683 681 479 239 234 219 191 156 122 118 SfoI GGC'GCC Cuts at: 2836 2836 Size: 8320 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8320 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8320 SmaI CCC'GGG Cuts at: 1872 2090 5306 5647 1872 Size: 218 3216 341 4545 Fragments arranged by size: 4545 3216 341 218 SmlI C'TyrA_G Cuts at: 820 1017 1943 2072 2576 3303 3326 3862 5133 Size: 197 926 129 504 727 23 536 1271 Cuts at: 5133 5498 6424 7325 7587 820 Size: 365 926 901 262 1553 Fragments arranged by size: 1553 1271 926 926 901 727 536 504 365 262 197 129 23 SnaBI TAC'GTA Cuts at: 493 6111 493 Size: 5618 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8320 SphI G_CATG'C Cuts at: 1132 2324 5678 1132 Size: 1192 3354 3774 Fragments arranged by size: 3774 3354 1192 SspI AAT'ATT Cuts at: 5 52 6244 5 Size: 47 6192 2081 Fragments arranged by size: 6192 2081 47 StuI AGG'CCT Cuts at: 1517 4451 1517 Size: 2934 5386 StyI C'CwwG_G Cuts at: 513 1065 2289 3762 3954 4864 4931 5232 5450 Size: 552 1224 1473 192 910 67 301 218 Cuts at: 5450 5469 5759 513 Size: 19 290 3074 Fragments arranged by size: 3074 1473 1224 910 552 301 290 218 192 67 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2098 4159 5462 6347 6364 6517 6702 Size: 1255 518 2061 1303 885 17 153 185 Cuts at: 6702 7147 325 Size: 445 1498 Fragments arranged by size: 2061 1498 1303 1255 885 518 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2719 6566 8278 Size: 274 80 33 51 1443 742 3847 1712 Cuts at: 8278 96 Size: 138 Fragments arranged by size: 3847 1712 1443 742 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4933 4965 5228 5260 8280 8312 4933 Size: 32 263 32 3020 32 4941 Fragments arranged by size: 4941 3020 263 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4841 5459 1316 Size: 3525 618 4177 Fragments arranged by size: 4177 3525 618 XbaI T'CTAG_A Cuts at: 5656 5656 Size: 8320 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2362 2732 2362 Size: 370 7950 XhoI C'TCGA_G Cuts at: 1943 5133 1943 Size: 3190 5130 XmaI C'CCGG_G Cuts at: 1870 2088 5304 5645 1870 Size: 218 3216 341 4545 Fragments arranged by size: 4545 3216 341 218 XmnI GAAnn'nnTTC Cuts at: 5402 6449 5402 Size: 1047 7273 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6128 276 Size: 53 83 186 936 4594 2468 Fragments arranged by size: 4594 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I FalI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SanDI SfiI SrfI SwaI