(Circular) (Six-base) MAPSORT of: pFN21AA1209.seq Check: 4115 from: 1 to: 9037 pFN21AA1209 9037 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6847 278 Size: 53 83 186 936 5311 2468 Fragments arranged by size: 5311 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6380 1937 Size: 4443 4594 Acc65I G'GTAC_C Cuts at: 6358 6358 Size: 9037 AclI AA'CG_TT Cuts at: 1563 7164 7537 1563 Size: 5601 373 3063 Fragments arranged by size: 5601 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 2848 3310 3669 7100 8484 8752 1632 Size: 563 653 462 359 3431 1384 268 1917 Fragments arranged by size: 3431 1917 1384 653 563 462 359 268 AfeI AGC'GCT Cuts at: 5698 5698 Size: 9037 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8840 AflIII A'CryG_T Cuts at: 1203 3249 7936 8913 1203 Size: 2046 4687 977 1327 Fragments arranged by size: 4687 2046 1327 977 AgeI A'CCGG_T Cuts at: 7868 7868 Size: 9037 AhdI GACnn_n'nnGTC Cuts at: 5497 7766 5497 Size: 2269 6768 AleI CACnn'nnGTG Cuts at: 1166 4886 1166 Size: 3720 5317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2058 2396 3305 6158 1184 Size: 874 338 909 2853 4063 Fragments arranged by size: 4063 2853 909 874 338 AlwNI CAG_nnn'CTG Cuts at: 5603 5800 5803 6780 8352 5603 Size: 197 3 977 1572 6288 Fragments arranged by size: 6288 1572 977 197 3 ApaI G_GGCC'C Cuts at: 5268 5268 Size: 9037 ApaLI G'TGCA_C Cuts at: 7094 8250 7094 Size: 1156 7881 ApoI r'AATT_y Cuts at: 1493 2541 2858 5340 5941 6305 6346 6572 6724 Size: 1048 317 2482 601 364 41 226 152 Cuts at: 6724 8833 1493 Size: 2109 1697 Fragments arranged by size: 2482 2109 1697 1048 601 364 317 226 152 41 AseI AT'TA_AT Cuts at: 160 1032 7591 160 Size: 872 6559 1606 Fragments arranged by size: 6559 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9037 AvaI C'yCGr_G Cuts at: 1870 1943 6362 1870 Size: 73 4419 4545 Fragments arranged by size: 4545 4419 73 BamHI G'GATC_C Cuts at: 6367 6734 6367 Size: 367 8670 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2088 2473 4337 5276 6358 Size: 325 217 627 301 385 1864 939 1082 Cuts at: 6358 7813 618 Size: 1455 1842 Fragments arranged by size: 1864 1842 1455 1082 939 627 385 325 301 217 BanII G_rGCy'C Cuts at: 1391 2190 2507 3766 4316 4422 5268 6356 1391 Size: 799 317 1259 550 106 846 1088 4072 Fragments arranged by size: 4072 1259 1088 846 799 550 317 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2183 3325 3626 3675 4737 4944 928 Size: 971 284 1142 301 49 1062 207 5021 Fragments arranged by size: 5021 1142 1062 971 301 284 207 49 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7228 7262 1903 Size: 34 5291 34 3678 Fragments arranged by size: 5291 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6929 8139 6929 Size: 1210 7827 BclI T'GATC_A Cuts at: 1548 3738 5745 1548 Size: 2190 2007 4840 Fragments arranged by size: 4840 2190 2007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2301 4792 7648 Size: 107 122 71 631 605 629 2491 2856 Cuts at: 7648 136 Size: 1525 Fragments arranged by size: 2856 2491 1525 631 629 605 122 107 71 BglII A'GATC_T Cuts at: 3889 5896 9032 3889 Size: 2007 3136 3894 Fragments arranged by size: 3894 3136 2007 BlpI GC'TnA_GC Cuts at: 3320 4203 4820 6454 3320 Size: 883 617 1634 5903 Fragments arranged by size: 5903 1634 883 617 Bme1580I G_kGCm'C Cuts at: 1790 2091 2476 5268 5882 7098 8254 1790 Size: 301 385 2792 614 1216 1156 2573 Fragments arranged by size: 2792 2573 1216 1156 614 385 301 BmgBI CAC'GTC Cuts at: 1318 4831 1318 Size: 3513 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2782 4623 5293 Size: 416 53 83 399 24 1365 1841 670 Cuts at: 5293 5719 6768 7726 442 Size: 426 1049 958 1753 Fragments arranged by size: 1841 1753 1365 1049 958 670 426 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9037 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4766 4890 4766 Size: 124 8913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 3449 3961 4514 4535 5198 5204 7697 Size: 1124 1086 512 553 21 663 6 2493 Cuts at: 7697 1239 Size: 2579 Fragments arranged by size: 2579 2493 1124 1086 663 553 512 21 6 Bpu10I CC'TnA_GC Cuts at: 3456 4815 3456 Size: 1359 7678 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2151 2924 3179 5352 5515 7162 8027 8325 2151 Size: 773 255 2173 163 1647 865 298 2863 Fragments arranged by size: 2863 2173 1647 865 773 298 255 163 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3965 4737 7700 882 Size: 617 2466 772 2963 2219 Fragments arranged by size: 2963 2466 2219 772 617 BsaAI yAC'GTr Cuts at: 493 1204 6828 493 Size: 711 5624 2702 Fragments arranged by size: 5624 2702 711 BsaBI GATnn'nnATC Cuts at: 3855 9031 3855 Size: 5176 3861 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6844 7226 7861 275 Size: 53 83 186 936 5311 382 635 1451 Fragments arranged by size: 5311 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3084 7470 7868 8142 8289 8700 3084 Size: 4386 398 274 147 411 3421 Fragments arranged by size: 4386 3421 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4021 4051 1178 Size: 30 165 30 2618 30 6164 Fragments arranged by size: 6164 2618 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2504 3874 4118 4409 4868 1186 Size: 1318 1370 244 291 459 5355 Fragments arranged by size: 5355 1370 1318 459 291 244 BseYI C'CCAG_C Cuts at: 3381 3675 3812 4016 4438 5355 8240 8736 3381 Size: 294 137 204 422 917 2885 496 3682 Fragments arranged by size: 3682 2885 917 496 422 294 204 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3392 4471 4781 5627 1163 Size: 573 1656 1079 310 846 4573 Fragments arranged by size: 4573 1656 1079 846 573 310 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2487 6487 7248 8276 1540 Size: 275 186 486 4000 761 1028 2301 Fragments arranged by size: 4000 2301 1028 761 486 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 3078 3766 4268 4481 4811 7098 7183 8254 Size: 888 688 502 213 330 2287 85 1071 Cuts at: 8254 2190 Size: 2973 Fragments arranged by size: 2973 2287 1071 888 688 502 330 213 85 BsmI GAATG_Cn' Cuts at: 5437 6145 6548 6641 5437 Size: 708 403 93 7833 Fragments arranged by size: 7833 708 403 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3702 4754 3702 Size: 1052 7985 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2190 2476 2507 3078 3766 4268 Size: 399 301 99 286 31 571 688 502 Cuts at: 4268 4316 4422 4481 4811 5268 5882 6356 7098 Size: 48 106 59 330 457 614 474 742 Cuts at: 7098 7183 8254 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 688 614 571 502 474 457 399 330 301 286 106 99 85 59 48 31 BspEI T'CCGG_A Cuts at: 8700 8700 Size: 9037 BspHI T'CATG_A Cuts at: 6926 8656 6926 Size: 1730 7307 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6392 844 Size: 5548 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3196 3319 3755 6924 1394 Size: 429 1373 123 436 3169 3507 Fragments arranged by size: 3507 3169 1373 436 429 123 BsrDI GCAATG_nn' Cuts at: 65 1248 6140 7532 7706 65 Size: 1183 4892 1392 174 1396 Fragments arranged by size: 4892 1396 1392 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4977 5194 6178 7681 7868 1700 Size: 110 3167 217 984 1503 187 2869 Fragments arranged by size: 3167 2869 1503 984 217 187 110 BsrGI T'GTAC_A Cuts at: 96 2566 96 Size: 2470 6567 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9037 BssSI C'ACGA_G Cuts at: 2500 3071 7097 8109 2500 Size: 571 4026 1012 3428 Fragments arranged by size: 4026 3428 1012 571 BstAPI GCAn_nnn'nTGC Cuts at: 794 2095 4032 5800 794 Size: 1301 1937 1768 4031 Fragments arranged by size: 4031 1937 1768 1301 BstBI TT'CG_AA Cuts at: 7841 7841 Size: 9037 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9037 BstXI CCAn_nnnn'nTGG Cuts at: 2341 5420 2341 Size: 3079 5958 BstYI r'GATC_y Cuts at: 1972 3668 3889 4426 5896 6367 6734 7119 7136 Size: 1696 221 537 1470 471 367 385 17 Cuts at: 7136 8588 8674 8686 9032 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1696 1470 1452 537 471 385 367 346 221 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3519 4523 5384 3519 Size: 1004 861 7172 Fragments arranged by size: 7172 1004 861 BtgI C'CryG_G Cuts at: 513 1065 2004 2254 2400 2814 5413 513 Size: 552 939 250 146 414 2599 4137 Fragments arranged by size: 4137 2599 939 552 414 250 146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8298 Fragments arranged by size: 8298 683 56 BtsI GCAGTG_nn' Cuts at: 2456 4018 6553 7347 7367 2456 Size: 1562 2535 794 20 4126 Fragments arranged by size: 4126 2535 1562 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2796 2831 6081 6116 529 Size: 35 2232 35 3250 35 3450 Fragments arranged by size: 3450 3250 2232 35 35 35 DraI TTT'AAA Cuts at: 2577 5163 6341 2577 Size: 2586 1178 5273 Fragments arranged by size: 5273 2586 1178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2207 8044 809 Size: 507 891 5837 1802 Fragments arranged by size: 5837 1802 891 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2496 2736 2853 Size: 54 1425 50 275 18 666 240 117 Cuts at: 2853 4300 5028 5196 6484 7373 9002 8 Size: 1447 728 168 1288 889 1629 43 Fragments arranged by size: 1629 1447 1425 1288 889 728 666 275 240 168 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6484 1537 Size: 275 4672 4090 Fragments arranged by size: 4672 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2245 2763 3209 3215 3233 4886 5241 Size: 671 221 518 446 6 18 1653 355 Cuts at: 5241 5580 6967 1353 Size: 339 1387 3423 Fragments arranged by size: 3423 1653 1387 671 518 446 355 339 221 18 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2041 7624 7998 8144 387 Size: 1064 138 452 5583 374 146 1280 Fragments arranged by size: 5583 1280 1064 452 374 146 138 EcoICRI GAG'CTC Cuts at: 2188 3764 2188 Size: 1576 7461 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2363 2848 3310 3449 3669 3961 Size: 393 563 168 485 462 139 220 292 Cuts at: 3961 4514 4535 5198 5204 7100 7697 8484 8752 Size: 553 21 663 6 1896 597 787 268 Cuts at: 8752 1239 Size: 1524 Fragments arranged by size: 1896 1524 787 663 597 563 553 485 462 393 292 268 220 168 139 21 6 EcoNI CCTnn'n_nnAGG Cuts at: 2981 3522 4044 5595 2981 Size: 541 522 1551 6423 Fragments arranged by size: 6423 1551 541 522 EcoO109I rG'GnC_Cy Cuts at: 2907 5264 5721 2907 Size: 2357 457 6223 Fragments arranged by size: 6223 2357 457 EcoRI G'AATT_C Cuts at: 6346 6724 6346 Size: 378 8659 EcoRV GAT'ATC Cuts at: 2677 5507 2677 Size: 2830 6207 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3491 3491 Size: 9037 FspI TGC'GCA Cuts at: 7543 7543 Size: 9037 HaeII r_GCGC'y Cuts at: 1678 4840 5700 8184 1678 Size: 3162 860 2484 2531 Fragments arranged by size: 3162 2531 2484 860 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3653 3685 3700 3732 3881 Size: 32 135 32 2081 32 15 32 149 Cuts at: 3881 3913 4418 4450 4658 4690 5483 5515 7678 Size: 32 505 32 208 32 793 32 2163 Cuts at: 7678 7710 7752 7784 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2163 2081 793 505 208 149 135 42 32 32 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 4137 6184 6381 6627 669 Size: 988 281 2199 2047 197 246 3079 Fragments arranged by size: 3079 2199 2047 988 281 246 197 HindIII A'AGCT_T Cuts at: 748 748 Size: 9037 HpaI GTT'AAC Cuts at: 6184 6627 6184 Size: 443 8594 KpnI G_GTAC'C Cuts at: 6362 6362 Size: 9037 MfeI C'AATT_G Cuts at: 5546 6636 5546 Size: 1090 7947 MluI A'CGCG_T Cuts at: 8913 8913 Size: 9037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5663 6030 6036 8151 8335 1305 Size: 4358 367 6 2115 184 2007 Fragments arranged by size: 4358 2115 2007 367 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 2738 2855 4302 5030 10 Size: 54 1425 343 906 117 1447 728 4017 Fragments arranged by size: 4017 1447 1425 906 728 343 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 3051 3293 4161 4886 5418 6995 7354 Size: 648 1885 242 868 725 532 1577 359 Cuts at: 7354 7513 518 Size: 159 2042 Fragments arranged by size: 2042 1885 1577 868 725 648 532 359 242 159 MspA1I CmG'CkG Cuts at: 1606 2117 2932 3679 3942 5800 6132 6453 7130 Size: 511 815 747 263 1858 332 321 677 Cuts at: 7130 8278 8523 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1858 1148 815 747 677 511 332 321 263 245 NaeI GCC'GGC Cuts at: 5196 5196 Size: 9037 NcoI C'CATG_G Cuts at: 513 1065 2004 2254 5413 513 Size: 552 939 250 3159 4137 Fragments arranged by size: 4137 3159 939 552 250 NdeI CA'TA_TG Cuts at: 387 3948 387 Size: 3561 5476 NgoMIV G'CCGG_C Cuts at: 5194 5194 Size: 9037 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9037 NotI GC'GGCC_GC Cuts at: 6484 6484 Size: 9037 NspI r_CATG'y Cuts at: 1132 1229 4035 6395 7940 1132 Size: 97 2806 2360 1545 2229 Fragments arranged by size: 2806 2360 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2632 1348 Size: 1284 7753 PciI A'CATG_T Cuts at: 7936 7936 Size: 9037 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9037 PmeI GTTT'AAAC Cuts at: 6341 6341 Size: 9037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7106 7138 8619 8651 7106 Size: 32 1481 32 7492 Fragments arranged by size: 7492 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2907 5721 2907 Size: 2814 6223 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9037 PsiI TTA'TAA Cuts at: 6607 6607 Size: 9037 PspOMI G'GGCC_C Cuts at: 5264 5264 Size: 9037 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2112 2144 2112 Size: 32 9005 PstI C_TGCA'G Cuts at: 830 2385 3360 4077 6131 6389 830 Size: 1555 975 717 2054 258 3478 Fragments arranged by size: 3478 2054 1555 975 717 258 PvuI CG_AT'CG Cuts at: 2001 2487 2001 Size: 486 8551 PvuII CAG'CTG Cuts at: 2117 3679 3942 5800 2117 Size: 1562 263 1858 5354 Fragments arranged by size: 5354 1858 1562 263 RsrII CG'GwC_CG Cuts at: 3082 3082 Size: 9037 SacI G_AGCT'C Cuts at: 2190 3766 2190 Size: 1576 7461 SalI G'TCGA_C Cuts at: 1936 6379 1936 Size: 4443 4594 SapI GCTCTTCn'nnn_ Cuts at: 3209 4886 3209 Size: 1677 7360 SbfI CC_TGCA'GG Cuts at: 6389 6389 Size: 9037 ScaI AGT'ACT Cuts at: 5536 7285 8997 5536 Size: 1749 1712 5576 Fragments arranged by size: 5576 1749 1712 SfcI C'TryA_G Cuts at: 826 1045 2381 3018 3356 4073 5446 6127 6385 Size: 219 1336 637 338 717 1373 681 258 Cuts at: 6385 7520 8201 8392 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1373 1336 1135 717 681 681 637 338 258 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9037 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9037 SmaI CCC'GGG Cuts at: 1872 6364 1872 Size: 4492 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2166 2939 3158 5367 5494 7141 Size: 197 926 223 773 219 2209 127 1647 Cuts at: 7141 8042 8304 820 Size: 901 262 1553 Fragments arranged by size: 2209 1647 1553 926 901 773 262 223 219 197 127 SnaBI TAC'GTA Cuts at: 493 6828 493 Size: 6335 2702 SpeI A'CTAG_T Cuts at: 152 4052 152 Size: 3900 5137 SphI G_CATG'C Cuts at: 1132 6395 1132 Size: 5263 3774 SspI AAT'ATT Cuts at: 5 52 6283 6961 5 Size: 47 6231 678 2081 Fragments arranged by size: 6231 2081 678 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9037 StyI C'CwwG_G Cuts at: 513 1065 2004 2254 2807 5413 5676 6476 513 Size: 552 939 250 553 2606 263 800 3074 Fragments arranged by size: 3074 2606 939 800 553 552 263 250 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2217 2455 2491 2654 3797 7064 7081 Size: 1255 637 238 36 163 1143 3267 17 Cuts at: 7081 7234 7419 7864 325 Size: 153 185 445 1498 Fragments arranged by size: 3267 1498 1255 1143 637 445 238 185 163 153 36 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2566 5127 5534 Size: 274 80 33 51 1443 589 2561 407 Cuts at: 5534 7283 8995 96 Size: 1749 1712 138 Fragments arranged by size: 2561 1749 1712 1443 589 407 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2044 2076 2788 2820 3627 3659 4021 4053 8997 Size: 32 712 32 807 32 362 32 4944 Cuts at: 8997 9029 2044 Size: 32 2052 Fragments arranged by size: 4944 2052 807 712 362 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4157 1316 Size: 2841 6196 XbaI T'CTAG_A Cuts at: 3656 6373 3656 Size: 2717 6320 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5411 5417 5411 Size: 6 9031 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9037 XmaI C'CCGG_G Cuts at: 1870 6362 1870 Size: 4492 4545 XmnI GAAnn'nnTTC Cuts at: 6306 7166 6306 Size: 860 8177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6845 276 Size: 53 83 186 936 5311 2468 Fragments arranged by size: 5311 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI SacII SanDI SexAI SfiI SfoI SrfI SwaI