(Circular) (Six-base) MAPSORT of: pFN21AB7820.seq Check: 3299 from: 1 to: 6502 pFN21AB7820 6502 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2931 2931 Size: 6502 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4312 278 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2217 3845 1937 Size: 280 1628 4594 Fragments arranged by size: 4594 1628 280 Acc65I G'GTAC_C Cuts at: 3092 3250 3823 3092 Size: 158 573 5771 Fragments arranged by size: 5771 573 158 AclI AA'CG_TT Cuts at: 1563 4629 5002 1563 Size: 3066 373 3063 Fragments arranged by size: 3066 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 2666 2699 3581 4565 5949 6217 1632 Size: 731 303 33 882 984 1384 268 1917 Fragments arranged by size: 1917 1384 984 882 731 303 268 33 AflII C'TTAA_G Cuts at: 820 1017 3644 820 Size: 197 2627 3678 Fragments arranged by size: 3678 2627 197 AflIII A'CryG_T Cuts at: 1203 5401 6378 1203 Size: 4198 977 1327 Fragments arranged by size: 4198 1327 977 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 5231 5231 Size: 6502 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6502 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2598 1184 Size: 1414 5088 AlwNI CAG_nnn'CTG Cuts at: 2266 2753 3397 4245 5817 2266 Size: 487 644 848 1572 2951 Fragments arranged by size: 2951 1572 848 644 487 ApaLI G'TGCA_C Cuts at: 4559 5715 4559 Size: 1156 5346 ApoI r'AATT_y Cuts at: 1493 3261 3605 3696 3811 4037 4189 6298 1493 Size: 1768 344 91 115 226 152 2109 1697 Fragments arranged by size: 2109 1768 1697 344 226 152 115 91 AseI AT'TA_AT Cuts at: 160 1032 5056 160 Size: 872 4024 1606 Fragments arranged by size: 4024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 2059 2420 3827 1870 Size: 73 116 361 1407 4545 Fragments arranged by size: 4545 1407 361 116 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3084 3117 3084 Size: 33 6469 BamHI G'GATC_C Cuts at: 2391 3114 3832 4199 2391 Size: 723 718 367 4694 Fragments arranged by size: 4694 723 718 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 3092 3250 3823 5278 Size: 325 217 627 261 1044 158 573 1455 Cuts at: 5278 618 Size: 1842 Fragments arranged by size: 1842 1455 1044 627 573 325 261 217 158 BanII G_rGCy'C Cuts at: 1391 2109 3477 3821 1391 Size: 718 1368 344 4072 Fragments arranged by size: 4072 1368 718 344 BbeI G_GCGC'C Cuts at: 2052 2052 Size: 6502 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5531 BbvCI CC'TCA_GC Cuts at: 2667 2667 Size: 6502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4693 4727 1903 Size: 34 2756 34 3678 Fragments arranged by size: 3678 2756 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4394 5604 4394 Size: 1210 5292 BclI T'GATC_A Cuts at: 1548 2130 1548 Size: 582 5920 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2877 3050 5113 Size: 107 122 71 631 605 1205 173 2063 Cuts at: 5113 136 Size: 1525 Fragments arranged by size: 2063 1525 1205 631 605 173 122 107 71 BglII A'GATC_T Cuts at: 6497 6497 Size: 6502 BlpI GC'TnA_GC Cuts at: 3919 3919 Size: 6502 Bme1580I G_kGCm'C Cuts at: 1790 4563 5719 1790 Size: 2773 1156 2573 Fragments arranged by size: 2773 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6502 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4233 5191 442 Size: 416 53 83 399 24 2816 958 1753 Fragments arranged by size: 2816 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6502 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3147 3147 Size: 6502 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2007 3067 3491 5162 1239 Size: 768 1060 424 1671 2579 Fragments arranged by size: 2579 1671 1060 768 424 Bpu10I CC'TnA_GC Cuts at: 2553 2667 2553 Size: 114 6388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2285 4627 5492 5790 2285 Size: 2342 865 298 2997 Fragments arranged by size: 2997 2342 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5165 882 Size: 617 3666 2219 Fragments arranged by size: 3666 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4293 493 Size: 711 3089 2702 Fragments arranged by size: 3089 2702 711 BsaBI GATnn'nnATC Cuts at: 6496 6496 Size: 6502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2055 4309 4691 Size: 53 83 186 936 516 6 2254 382 Cuts at: 4691 5326 275 Size: 635 1451 Fragments arranged by size: 2254 1451 936 635 516 382 186 83 53 6 BsaWI w'CCGG_w Cuts at: 2388 4935 5333 5607 5754 6165 2388 Size: 2547 398 274 147 411 2725 Fragments arranged by size: 2725 2547 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3362 3392 1178 Size: 30 165 30 1959 30 4288 Fragments arranged by size: 4288 1959 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3720 1186 Size: 2534 3968 BseYI C'CCAG_C Cuts at: 2404 5705 6201 2404 Size: 3301 496 2705 Fragments arranged by size: 3301 2705 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2811 1163 Size: 573 1075 4854 Fragments arranged by size: 4854 1075 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3952 4713 5741 1540 Size: 275 186 1951 761 1028 2301 Fragments arranged by size: 2301 1951 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2893 3477 4563 4648 5719 2893 Size: 584 1086 85 1071 3676 Fragments arranged by size: 3676 1086 1071 584 85 BsmI GAATG_Cn' Cuts at: 4013 4106 4013 Size: 93 6409 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2893 3477 3821 4563 4648 5719 Size: 399 319 784 584 344 742 85 1071 Cuts at: 5719 1391 Size: 2174 Fragments arranged by size: 2174 1071 784 742 584 399 344 319 85 BspEI T'CCGG_A Cuts at: 2388 6165 2388 Size: 3777 2725 BspHI T'CATG_A Cuts at: 3733 4391 6121 3733 Size: 658 1730 4114 Fragments arranged by size: 4114 1730 658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2374 2931 3857 844 Size: 1530 557 926 3489 Fragments arranged by size: 3489 1530 926 557 BsrBI CCG'CTC Cuts at: 1394 1823 3112 4389 1394 Size: 429 1289 1277 3507 Fragments arranged by size: 3507 1289 1277 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3018 3314 4997 5171 65 Size: 1183 1770 296 1683 174 1396 Fragments arranged by size: 1770 1683 1396 1183 296 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 5146 5333 1700 Size: 110 241 3095 187 2869 Fragments arranged by size: 3095 2869 241 187 110 BsrGI T'GTAC_A Cuts at: 96 2331 96 Size: 2235 4267 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6502 BssSI C'ACGA_G Cuts at: 4562 5574 4562 Size: 1012 5490 BstAPI GCAn_nnn'nTGC Cuts at: 794 3002 3316 794 Size: 2208 314 3980 Fragments arranged by size: 3980 2208 314 BstBI TT'CG_AA Cuts at: 2460 5306 2460 Size: 2846 3656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6502 BstXI CCAn_nnnn'nTGG Cuts at: 2938 2938 Size: 6502 BstYI r'GATC_y Cuts at: 1972 2391 2941 3114 3832 4199 4584 4601 6053 Size: 419 550 173 718 367 385 17 1452 Cuts at: 6053 6139 6151 6497 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 718 550 419 385 367 346 173 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3674 3674 Size: 6502 BtgI C'CryG_G Cuts at: 513 1065 2004 2364 2398 3006 3366 513 Size: 552 939 360 34 608 360 3649 Fragments arranged by size: 3649 939 608 552 360 360 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2675 2687 487 Size: 683 56 1449 12 4302 Fragments arranged by size: 4302 1449 683 56 12 BtsI GCAGTG_nn' Cuts at: 2906 3202 4018 4812 4832 2906 Size: 296 816 794 20 4576 Fragments arranged by size: 4576 816 794 296 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6502 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6467 DraI TTT'AAA Cuts at: 3806 3806 Size: 6502 DraIII CAC_nnn'GTG Cuts at: 2311 2329 2311 Size: 18 6484 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5509 809 Size: 507 4193 1802 Fragments arranged by size: 4193 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2257 2869 3949 Size: 54 1425 50 275 18 427 612 1080 Cuts at: 3949 4838 6467 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1080 889 612 427 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3949 1537 Size: 275 2137 4090 Fragments arranged by size: 4090 2137 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2339 2492 2718 3144 3184 3240 4432 1353 Size: 986 153 226 426 40 56 1192 3423 Fragments arranged by size: 3423 1192 986 426 226 153 56 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5089 5463 5609 387 Size: 1064 138 3500 374 146 1280 Fragments arranged by size: 3500 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3475 3475 Size: 6502 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2363 2666 2699 3067 3491 3581 Size: 393 375 356 303 33 368 424 90 Cuts at: 3581 4565 5162 5949 6217 1239 Size: 984 597 787 268 1524 Fragments arranged by size: 1524 984 787 597 424 393 375 368 356 303 268 90 33 EcoNI CCTnn'n_nnAGG Cuts at: 3563 3563 Size: 6502 EcoO109I rG'GnC_Cy Cuts at: 2547 2547 Size: 6502 EcoRI G'AATT_C Cuts at: 3696 3811 4189 3696 Size: 115 378 6009 Fragments arranged by size: 6009 378 115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3298 3298 Size: 6502 FspI TGC'GCA Cuts at: 5008 5008 Size: 6502 HaeII r_GCGC'y Cuts at: 1678 2052 3559 3622 5649 1678 Size: 374 1507 63 2027 2531 Fragments arranged by size: 2531 2027 1507 374 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2800 2832 2947 2950 2979 Size: 32 135 32 1228 32 115 3 29 Cuts at: 2979 2982 3013 3045 3058 3090 3211 3243 3344 Size: 3 31 32 13 32 121 32 101 Cuts at: 3344 3376 3459 3491 5143 5175 5217 5249 1373 Size: 32 83 32 1652 32 42 32 2626 Fragments arranged by size: 2626 1652 1228 135 121 115 101 83 42 32 32 32 32 32 32 32 32 32 32 31 29 13 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2218 2527 3381 3846 4092 669 Size: 988 281 280 309 854 465 246 3079 Fragments arranged by size: 3079 988 854 465 309 281 280 246 HindIII A'AGCT_T Cuts at: 748 2693 748 Size: 1945 4557 HpaI GTT'AAC Cuts at: 2527 4092 2527 Size: 1565 4937 KasI G'GCGC_C Cuts at: 2048 2048 Size: 6502 KpnI G_GTAC'C Cuts at: 3096 3254 3827 3096 Size: 158 573 5771 Fragments arranged by size: 5771 573 158 MfeI C'AATT_G Cuts at: 4101 4101 Size: 6502 MluI A'CGCG_T Cuts at: 6378 6378 Size: 6502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2663 2918 5616 5800 1305 Size: 1358 255 2698 184 2007 Fragments arranged by size: 2698 2007 1358 255 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2871 10 Size: 54 1425 343 1039 3641 Fragments arranged by size: 3641 1425 1039 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3011 3738 4460 4819 4978 518 Size: 648 1845 727 722 359 159 2042 Fragments arranged by size: 2042 1845 727 722 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2046 2400 2433 2654 3298 3323 3918 4595 Size: 440 354 33 221 644 25 595 677 Cuts at: 4595 5743 5988 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 644 595 440 354 245 221 33 25 NaeI GCC'GGC Cuts at: 2053 2053 Size: 6502 NarI GG'CG_CC Cuts at: 2049 2049 Size: 6502 NcoI C'CATG_G Cuts at: 513 1065 2004 3006 513 Size: 552 939 1002 4009 Fragments arranged by size: 4009 1002 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6502 NgoMIV G'CCGG_C Cuts at: 2051 2051 Size: 6502 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6502 NotI GC'GGCC_GC Cuts at: 3949 3949 Size: 6502 NspI r_CATG'y Cuts at: 1132 1229 2224 3860 5405 1132 Size: 97 995 1636 1545 2229 Fragments arranged by size: 2229 1636 1545 995 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6502 PciI A'CATG_T Cuts at: 5401 5401 Size: 6502 PflMI CCAn_nnn'nTGG Cuts at: 1108 2882 1108 Size: 1774 4728 PfoI T'CCnGG_A Cuts at: 3429 3429 Size: 6502 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4571 4603 6084 6116 4571 Size: 32 1481 32 4957 Fragments arranged by size: 4957 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 3104 4072 3104 Size: 968 5534 PstI C_TGCA'G Cuts at: 830 2659 2913 3061 3854 830 Size: 1829 254 148 793 3478 Fragments arranged by size: 3478 1829 793 254 148 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 PvuII CAG'CTG Cuts at: 2654 3298 2654 Size: 644 5858 SacI G_AGCT'C Cuts at: 3477 3477 Size: 6502 SacII CC_GC'GG Cuts at: 2401 2401 Size: 6502 SalI G'TCGA_C Cuts at: 1936 2216 3844 1936 Size: 280 1628 4594 Fragments arranged by size: 4594 1628 280 SbfI CC_TGCA'GG Cuts at: 3854 3854 Size: 6502 ScaI AGT'ACT Cuts at: 4750 6462 4750 Size: 1712 4790 SexAI A'CCwGG_T Cuts at: 2307 2307 Size: 6502 SfcI C'TryA_G Cuts at: 826 1045 2655 2909 3057 3478 3850 4985 5666 Size: 219 1610 254 148 421 372 1135 681 Cuts at: 5666 5857 826 Size: 191 1471 Fragments arranged by size: 1610 1471 1135 681 421 372 254 219 191 148 SfoI GGC'GCC Cuts at: 2050 2050 Size: 6502 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 2051 1700 Size: 351 6151 SmaI CCC'GGG Cuts at: 1872 2061 2422 3829 1872 Size: 189 361 1407 4545 Fragments arranged by size: 4545 1407 361 189 SmlI C'TyrA_G Cuts at: 820 1017 1943 2300 3644 4606 5507 5769 820 Size: 197 926 357 1344 962 901 262 1553 Fragments arranged by size: 1553 1344 962 926 901 357 262 197 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 3659 152 Size: 3507 2995 SphI G_CATG'C Cuts at: 1132 3860 1132 Size: 2728 3774 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 2613 1517 Size: 1096 5406 StyI C'CwwG_G Cuts at: 513 1065 2004 3006 3282 3600 3941 513 Size: 552 939 1002 276 318 341 3074 Fragments arranged by size: 3074 1002 939 552 341 318 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2756 4529 4546 4699 4884 5329 325 Size: 1255 1176 1773 17 153 185 445 1498 Fragments arranged by size: 1773 1498 1255 1176 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2088 2331 2372 Size: 274 80 33 51 1443 111 243 41 Cuts at: 2372 4748 6460 96 Size: 2376 1712 138 Fragments arranged by size: 2376 1712 1443 274 243 138 111 80 51 41 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6462 6494 6462 Size: 32 6470 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6502 XbaI T'CTAG_A Cuts at: 3145 3838 3145 Size: 693 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2879 2879 Size: 6502 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6502 XmaI C'CCGG_G Cuts at: 1870 2059 2420 3827 1870 Size: 189 361 1407 4545 Fragments arranged by size: 4545 1407 361 189 XmnI GAAnn'nnTTC Cuts at: 2456 4631 2456 Size: 2175 4327 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4310 276 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI