################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AviII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI Bsp1407I BspEI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Cfr10I CpoI DraIII EagI EclXI Eco47III Eco52I FseI FspI HaeII KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 526 GACGTC GACGT^C AccI 365 GTAGAC GT^[AC][GT]AC AhdI 622 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1838 CCTCAGG CC^T[ACGT]AGG AspEI 622 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2929 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3291 CCTAGG C^CTAGG BalI 1224 TGGCCA TGG^CCA BamHI 3652 GGATCC G^GATCC BlnI 3291 CCTAGG C^CTAGG BlpI 1053 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1053 GCTTAGC GC^T[ACGT]AGC BsgI 1436 GTGCAG GTGCAG(16/14) BsmBI 3209 CGTCTC CGTCTC(1/5) BsmI 392 GCATTC GAATGC(1/-1) BspDI 260 ATCGAT AT^CGAT BsrBI 1278 CCGCTC CCGCTC(-3/-3) BssSI 3249 CACGAG CACGAG(-5/-1) BstAPI 1855 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1262 GGTCACC G^GT[ACGT]ACC BstPI 1262 GGTCACC G^GT[ACGT]ACC Bsu36I 1838 CCTCAGG CC^T[ACGT]AGG CelII 1053 GCTTAGC GC^T[ACGT]AGC CfoI 1972 GCGC GCG^C ClaI 260 ATCGAT AT^CGAT DraI 1824 TTTAAA TTT^AAA DrdI 1118 GACACCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 622 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1838 CCTCAGG CC^T[ACGT]AGG EcoO65I 1262 GGTCACC G^GT[ACGT]ACC EcoRI 760 GAATTC G^AATTC EcoT22I 575 ATGCAT ATGCA^T FauI 3326 CCCGC CCCGC(4/6) HgaI 1950 GACGC GACGC(5/10) HhaI 1972 GCGC GCG^C HinP1I 1972 GCGC G^CGC HpaI 1612 GTTAAC GTT^AAC MfeI 974 CAATTG C^AATTG MluNI 1224 TGGCCA TGG^CCA MscI 1224 TGGCCA TGG^CCA MunI 974 CAATTG C^AATTG NsiI 575 ATGCAT ATGCA^T PaeR7I 539 CTCGAG C^TCGAG PflFI 2929 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 1862 CTGCAG CTGCA^G SacI 1620 GAGCTC GAGCT^C ScaI 1752 AGTACT AGT^ACT StuI 3253 AGGCCT AGG^CCT Tth111I 2929 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 539 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AciI 1278 CCGC CCGC(-3/-1) 3327 CCGC AcyI 526 GACGTC G[GA]^CG[CT]C 1950 GACGCC AlwNI 1382 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 2882 CAGTTTCTG AvaI 326 CCCGAG C^[CT]CG[GA]G 539 CTCGAG BanI 2499 GGTGCC G^G[CT][GA]CC 2776 GGTGCC BanII 1620 GAGCTC G[GA]GC[CT]^C 2095 GAGCCC BclI 1818 TGATCA T^GATCA 2280 TGATCA BcnI 2786 CCCGG CC^[GC]GG 3365 CCCGG BsaHI 526 GACGTC G[GA]^CG[CT]C 1950 GACGCC BsaI 1402 GAGACC GGTCTC(1/5) 2681 GAGACC BsoBI 326 CCCGAG C^[CT]CG[GA]G 539 CTCGAG BspHI 2214 TCATGA T^CATGA 2651 TCATGA BspMI 1405 ACCTGC ACCTGC(4/8) 550 GCAGGT BsrDI 3622 CATTGC GCAATG(2/0) 1595 GCAATG BstXI 1227 CCAGGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3166 CCAGTTTCATGG DsaI 1221 CCATGG C^C[GA][CT]GG 2090 CCATGG EaeI 1224 TGGCCA [CT]^GGCC[GA] 3226 CGGCCA EcoRV 25 GATATC GAT^ATC 2631 GATATC FbaI 1818 TGATCA T^GATCA 2280 TGATCA Hin1I 526 GACGTC G[GA]^CG[CT]C 1950 GACGCC MslI 642 CATGTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1516 CACTATGATG MspA1I 667 CAGCTG C[AC]G^C[GT]G 3104 CAGCTG NciI 2786 CCCGG CC^[GC]GG 3365 CCCGG NcoI 1221 CCATGG C^CATGG 2090 CCATGG PflMI 1252 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1646 CCAAAGAATGG PvuII 667 CAGCTG CAG^CTG 3104 CAGCTG RcaI 2214 TCATGA T^CATGA 2651 TCATGA SfcI 1706 CTGTAG C^T[GA][CT]AG 1862 CTGCAG Van91I 1252 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1646 CCAAAGAATGG XbaI 774 TCTAGA T^CTAGA 3142 TCTAGA XcmI 350 CCACTCTCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1955 CCACAAGTCTAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI