(Circular) (Six-base) MAPSORT of: pFN21AA0715.seq Check: 920 from: 1 to: 9088 pFN21AA0715 9088 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4228 4274 5837 6291 4228 Size: 46 1563 454 7025 Fragments arranged by size: 7025 1563 454 46 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3854 6898 278 Size: 53 83 186 936 2318 3044 2468 Fragments arranged by size: 3044 2468 2318 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4365 5582 6431 1937 Size: 2428 1217 849 4594 Fragments arranged by size: 4594 2428 1217 849 Acc65I G'GTAC_C Cuts at: 6409 6409 Size: 9088 AclI AA'CG_TT Cuts at: 1563 7215 7588 1563 Size: 5652 373 3063 Fragments arranged by size: 5652 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3595 4261 6118 7151 8535 8803 1632 Size: 1963 666 1857 1033 1384 268 1917 Fragments arranged by size: 1963 1917 1857 1384 1033 666 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8891 AflIII A'CryG_T Cuts at: 1203 2653 3102 5364 7987 8964 1203 Size: 1450 449 2262 2623 977 1327 Fragments arranged by size: 2623 2262 1450 1327 977 449 AgeI A'CCGG_T Cuts at: 7919 7919 Size: 9088 AhdI GACnn_n'nnGTC Cuts at: 7817 7817 Size: 9088 AleI CACnn'nnGTG Cuts at: 1166 4217 1166 Size: 3051 6037 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2464 4024 1184 Size: 1280 1560 6248 Fragments arranged by size: 6248 1560 1280 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2672 2704 2672 Size: 32 9056 AlwNI CAG_nnn'CTG Cuts at: 2217 3015 3136 3517 4633 4970 4985 6831 8403 Size: 798 121 381 1116 337 15 1846 1572 Cuts at: 8403 2217 Size: 2902 Fragments arranged by size: 2902 1846 1572 1116 798 381 337 121 15 ApaI G_GGCC'C Cuts at: 3475 3475 Size: 9088 ApaLI G'TGCA_C Cuts at: 4187 7145 8301 4187 Size: 2958 1156 4974 Fragments arranged by size: 4974 2958 1156 ApoI r'AATT_y Cuts at: 1493 2463 2750 6397 6623 6775 8884 1493 Size: 970 287 3647 226 152 2109 1697 Fragments arranged by size: 3647 2109 1697 970 287 226 152 AseI AT'TA_AT Cuts at: 160 1032 4431 7642 160 Size: 872 3399 3211 1606 Fragments arranged by size: 3399 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9088 AvaI C'yCGr_G Cuts at: 1870 1943 3179 3537 3944 6413 1870 Size: 73 1236 358 407 2469 4545 Fragments arranged by size: 4545 2469 1236 407 358 73 BamHI G'GATC_C Cuts at: 2970 3478 4728 6418 6785 2970 Size: 508 1250 1690 367 5273 Fragments arranged by size: 5273 1690 1250 508 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3541 3579 4262 4338 6409 Size: 325 217 627 1754 38 683 76 2071 Cuts at: 6409 7864 618 Size: 1455 1842 Fragments arranged by size: 2071 1842 1754 1455 683 627 325 217 76 38 BanII G_rGCy'C Cuts at: 1391 3256 3475 3807 4169 4465 5663 6158 6407 Size: 1865 219 332 362 296 1198 495 249 Cuts at: 6407 1391 Size: 4072 Fragments arranged by size: 4072 1865 1198 495 362 332 296 249 219 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2332 2801 4413 4680 5012 5753 928 Size: 971 433 469 1612 267 332 741 4263 Fragments arranged by size: 4263 1612 971 741 469 433 332 267 BbvCI CC'TCA_GC Cuts at: 3902 4581 6256 3902 Size: 679 1675 6734 Fragments arranged by size: 6734 1675 679 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2872 2906 7279 7313 1903 Size: 34 935 34 4373 34 3678 Fragments arranged by size: 4373 3678 935 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5818 6980 8190 5818 Size: 1162 1210 6716 Fragments arranged by size: 6716 1210 1162 BclI T'GATC_A Cuts at: 1548 2394 3117 1548 Size: 846 723 7519 Fragments arranged by size: 7519 846 723 BfrBI ATG'CAT Cuts at: 4001 4518 5609 4001 Size: 517 1091 7480 Fragments arranged by size: 7480 1091 517 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3968 7699 136 Size: 107 122 71 631 605 2296 3731 1525 Fragments arranged by size: 3731 2296 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 9083 9083 Size: 9088 BlpI GC'TnA_GC Cuts at: 3387 3510 6505 3387 Size: 123 2995 5970 Fragments arranged by size: 5970 2995 123 Bme1580I G_kGCm'C Cuts at: 1790 3088 3475 3538 3546 3582 4191 4265 7149 Size: 1298 387 63 8 36 609 74 2884 Cuts at: 7149 8305 1790 Size: 1156 2573 Fragments arranged by size: 2884 2573 1298 1156 609 387 74 63 36 8 BmgBI CAC'GTC Cuts at: 1318 2654 1318 Size: 1336 7752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3075 4674 5056 Size: 416 53 83 399 24 1658 1599 382 Cuts at: 5056 6101 6819 7777 442 Size: 1045 718 958 1753 Fragments arranged by size: 1753 1658 1599 1045 958 718 416 399 382 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9088 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3433 5792 3433 Size: 2359 6729 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2085 2176 3113 3416 3624 3878 4097 4655 Size: 846 91 937 303 208 254 219 558 Cuts at: 4655 4770 5087 5398 7748 1239 Size: 115 317 311 2350 2579 Fragments arranged by size: 2579 2350 937 846 558 317 311 303 254 219 208 115 91 Bpu10I CC'TnA_GC Cuts at: 3902 3955 4233 4581 5538 6244 6256 3902 Size: 53 278 348 957 706 12 6734 Fragments arranged by size: 6734 957 706 348 278 53 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2627 3217 3817 5270 6298 7213 8078 8376 2627 Size: 590 600 1453 1028 915 865 298 3339 Fragments arranged by size: 3339 1453 1028 915 865 600 590 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2194 3221 3392 3683 4838 7751 882 Size: 617 695 1027 171 291 1155 2913 2219 Fragments arranged by size: 2913 2219 1155 1027 695 617 291 171 BsaAI yAC'GTr Cuts at: 493 1204 6879 493 Size: 711 5675 2702 Fragments arranged by size: 5675 2702 711 BsaBI GATnn'nnATC Cuts at: 2975 3143 9082 2975 Size: 168 5939 2981 Fragments arranged by size: 5939 2981 168 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3851 6895 7277 7912 Size: 53 83 186 936 2318 3044 382 635 Cuts at: 7912 275 Size: 1451 Fragments arranged by size: 3044 2318 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 7521 7919 8193 8340 8751 4551 Size: 2970 398 274 147 411 4888 Fragments arranged by size: 4888 2970 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2672 2702 3281 3311 3517 Size: 30 165 30 1269 30 579 30 206 Cuts at: 3517 3547 3680 3710 4070 4100 5690 5720 1178 Size: 30 133 30 360 30 1590 30 4546 Fragments arranged by size: 4546 1590 1269 579 360 206 165 133 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2561 3535 3538 3727 4118 4274 5469 5768 Size: 1375 974 3 189 391 156 1195 299 Cuts at: 5768 6244 6364 1186 Size: 476 120 3910 Fragments arranged by size: 3910 1375 1195 974 476 391 299 189 156 120 3 BseYI C'CCAG_C Cuts at: 2557 4120 4411 4724 6286 8291 8787 2557 Size: 1563 291 313 1562 2005 496 2858 Fragments arranged by size: 2858 2005 1563 1562 496 313 291 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2203 2513 2809 3909 4030 4273 4608 Size: 573 467 310 296 1100 121 243 335 Cuts at: 4608 1163 Size: 5643 Fragments arranged by size: 5643 1100 573 467 335 310 296 243 121 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6538 7299 8327 1540 Size: 275 186 4537 761 1028 2301 Fragments arranged by size: 4537 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3511 4191 5778 6158 6282 6344 7149 7234 8305 Size: 680 1587 380 124 62 805 85 1071 Cuts at: 8305 3511 Size: 4294 Fragments arranged by size: 4294 1587 1071 805 680 380 124 85 62 BsmI GAATG_Cn' Cuts at: 5020 5609 6599 6692 5020 Size: 589 990 93 7416 Fragments arranged by size: 7416 990 589 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2873 2873 Size: 9088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3088 3256 3475 3511 3538 3546 3582 Size: 399 1298 168 219 36 27 8 36 Cuts at: 3582 3807 4169 4191 4265 4465 5663 5778 6158 Size: 225 362 22 74 200 1198 115 380 Cuts at: 6158 6282 6344 6407 7149 7234 8305 1391 Size: 124 62 63 742 85 1071 2174 Fragments arranged by size: 2174 1298 1198 1071 742 399 380 362 225 219 200 168 124 115 85 74 63 62 36 36 27 22 8 BspEI T'CCGG_A Cuts at: 4551 8751 4551 Size: 4200 4888 BspHI T'CATG_A Cuts at: 2391 5148 6977 8707 2391 Size: 2757 1829 1730 2772 Fragments arranged by size: 2772 2757 1829 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2225 3718 4228 4274 4407 5388 5837 6291 Size: 1381 1493 510 46 133 981 449 454 Cuts at: 6291 6443 844 Size: 152 3489 Fragments arranged by size: 3489 1493 1381 981 510 454 449 152 133 46 BsrBI CCG'CTC Cuts at: 1394 1823 2935 5091 6975 1394 Size: 429 1112 2156 1884 3507 Fragments arranged by size: 3507 2156 1884 1112 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2551 3208 3741 4518 4776 5531 5742 Size: 1183 1303 657 533 777 258 755 211 Cuts at: 5742 7583 7757 65 Size: 1841 174 1396 Fragments arranged by size: 1841 1396 1303 1183 777 755 657 533 258 211 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3561 4677 6336 7732 7919 1700 Size: 110 1751 1116 1659 1396 187 2869 Fragments arranged by size: 2869 1751 1659 1396 1116 187 110 BsrGI T'GTAC_A Cuts at: 96 5817 96 Size: 5721 3367 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9088 BssSI C'ACGA_G Cuts at: 5300 7148 8160 5300 Size: 1848 1012 6228 Fragments arranged by size: 6228 1848 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4515 6222 794 Size: 3721 1707 3660 Fragments arranged by size: 3721 3660 1707 BstBI TT'CG_AA Cuts at: 2461 2691 7892 2461 Size: 230 5201 3657 Fragments arranged by size: 5201 3657 230 BstEII G'GTnAC_C Cuts at: 1184 3817 1184 Size: 2633 6455 BstXI CCAn_nnnn'nTGG Cuts at: 3169 4132 5341 3169 Size: 963 1209 6916 Fragments arranged by size: 6916 1209 963 BstYI r'GATC_y Cuts at: 1972 2970 3282 3478 4728 6418 6785 7170 7187 Size: 998 312 196 1250 1690 367 385 17 Cuts at: 7187 8639 8725 8737 9083 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1690 1452 1250 998 385 367 346 312 196 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2331 5764 513 Size: 552 939 327 3433 3837 Fragments arranged by size: 3837 3433 939 552 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3247 6344 487 Size: 683 56 2021 3097 3231 Fragments arranged by size: 3231 3097 2021 683 56 BtsI GCAGTG_nn' Cuts at: 2048 3921 4018 6604 7398 7418 2048 Size: 1873 97 2586 794 20 3718 Fragments arranged by size: 3718 2586 1873 794 97 20 ClaI AT'CG_AT Cuts at: 1554 4998 1554 Size: 3444 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5410 5445 529 Size: 35 4846 35 4172 Fragments arranged by size: 4846 4172 35 35 DraI TTT'AAA Cuts at: 6392 6392 Size: 9088 DraIII CAC_nnn'GTG Cuts at: 6109 6222 6109 Size: 113 8975 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8095 809 Size: 507 6779 1802 Fragments arranged by size: 6779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 3969 4134 Size: 54 1425 50 275 18 540 1599 165 Cuts at: 4134 5308 5331 5554 6535 7424 9053 8 Size: 1174 23 223 981 889 1629 43 Fragments arranged by size: 1629 1599 1425 1174 981 889 540 275 223 165 54 50 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 6535 1537 Size: 275 4723 4090 Fragments arranged by size: 4723 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 2243 3219 3423 3881 4310 4536 4872 Size: 825 65 976 204 458 429 226 336 Cuts at: 4872 5102 5390 5465 5937 6180 7018 1353 Size: 230 288 75 472 243 838 3423 Fragments arranged by size: 3423 976 838 825 472 458 429 336 288 243 230 226 204 75 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7675 8049 8195 387 Size: 1064 138 6086 374 146 1280 Fragments arranged by size: 6086 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 6156 6156 Size: 9088 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2176 3113 3416 3595 3624 3878 Size: 393 453 91 937 303 179 29 254 Cuts at: 3878 4097 4261 4655 4770 5087 5398 6118 7151 Size: 219 164 394 115 317 311 720 1033 Cuts at: 7151 7748 8535 8803 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1033 937 787 720 597 453 394 393 317 311 303 268 254 219 179 164 115 91 29 EcoNI CCTnn'n_nnAGG Cuts at: 3128 4256 3128 Size: 1128 7960 EcoO109I rG'GnC_Cy Cuts at: 3024 3312 4117 4741 6290 3024 Size: 288 805 624 1549 5822 Fragments arranged by size: 5822 1549 805 624 288 EcoRI G'AATT_C Cuts at: 6397 6775 6397 Size: 378 8710 FspI TGC'GCA Cuts at: 4508 7594 4508 Size: 3086 6002 HaeII r_GCGC'y Cuts at: 1678 8235 1678 Size: 6557 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2514 2546 2590 2622 3680 Size: 32 135 32 942 32 44 32 1058 Cuts at: 3680 3712 3986 4018 4070 4102 4696 4728 4980 Size: 32 274 32 52 32 594 32 252 Cuts at: 4980 5012 6227 6259 6343 6375 7729 7761 7803 Size: 32 1215 32 84 32 1354 32 42 Cuts at: 7803 7835 1373 Size: 32 2626 Fragments arranged by size: 2626 1354 1215 1058 942 594 274 252 135 84 52 44 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4774 5377 5583 6432 6678 669 Size: 988 281 2836 603 206 849 246 3079 Fragments arranged by size: 3079 2836 988 849 603 281 246 206 HindIII A'AGCT_T Cuts at: 748 748 Size: 9088 HpaI GTT'AAC Cuts at: 6678 6678 Size: 9088 KpnI G_GTAC'C Cuts at: 6413 6413 Size: 9088 MfeI C'AATT_G Cuts at: 4864 6687 4864 Size: 1823 7265 MluI A'CGCG_T Cuts at: 8964 8964 Size: 9088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2478 3364 3666 6029 8202 8386 1305 Size: 1173 886 302 2363 2173 184 2007 Fragments arranged by size: 2363 2173 2007 1173 886 302 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2372 3971 4136 5310 5333 Size: 54 1425 343 540 1599 165 1174 23 Cuts at: 5333 5556 10 Size: 223 3542 Fragments arranged by size: 3542 1599 1425 1174 540 343 223 165 54 23 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2843 2981 3353 4006 4217 4433 Size: 648 1368 309 138 372 653 211 216 Cuts at: 4433 7046 7405 7564 518 Size: 2613 359 159 2042 Fragments arranged by size: 2613 2042 1368 653 648 372 359 309 216 211 159 138 MspA1I CmG'CkG Cuts at: 1606 2092 2140 4769 6504 7181 8329 8574 1606 Size: 486 48 2629 1735 677 1148 245 2120 Fragments arranged by size: 2629 2120 1735 1148 677 486 245 48 NcoI C'CATG_G Cuts at: 513 1065 2004 2331 513 Size: 552 939 327 7270 Fragments arranged by size: 7270 939 552 327 NdeI CA'TA_TG Cuts at: 387 387 Size: 9088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9088 NotI GC'GGCC_GC Cuts at: 6535 6535 Size: 9088 NsiI A_TGCA'T Cuts at: 4003 4520 5611 4003 Size: 517 1091 7480 Fragments arranged by size: 7480 1091 517 NspI r_CATG'y Cuts at: 1132 1229 3106 4057 5239 6182 6446 7991 1132 Size: 97 1877 951 1182 943 264 1545 2229 Fragments arranged by size: 2229 1877 1545 1182 951 943 264 97 PasI CC'CwG_GG Cuts at: 1348 4258 4294 1348 Size: 2910 36 6142 Fragments arranged by size: 6142 2910 36 PciI A'CATG_T Cuts at: 3102 7987 3102 Size: 4885 4203 PflMI CCAn_nnn'nTGG Cuts at: 1108 4349 4985 5737 1108 Size: 3241 636 752 4459 Fragments arranged by size: 4459 3241 752 636 PfoI T'CCnGG_A Cuts at: 2192 4074 4223 2192 Size: 1882 149 7057 Fragments arranged by size: 7057 1882 149 PmeI GTTT'AAAC Cuts at: 6392 6392 Size: 9088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2672 2704 7157 7189 8670 8702 2672 Size: 32 4453 32 1481 32 3058 Fragments arranged by size: 4453 3058 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3024 3312 4741 6290 3024 Size: 288 1429 1549 5822 Fragments arranged by size: 5822 1549 1429 288 PshAI GACnn'nnGTC Cuts at: 1613 4769 1613 Size: 3156 5932 PsiI TTA'TAA Cuts at: 6658 6658 Size: 9088 PspOMI G'GGCC_C Cuts at: 3471 3471 Size: 9088 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5354 5386 5354 Size: 32 9056 PstI C_TGCA'G Cuts at: 830 4686 4793 5518 6440 830 Size: 3856 107 725 922 3478 Fragments arranged by size: 3856 3478 922 725 107 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9088 RsrII CG'GwC_CG Cuts at: 5073 5073 Size: 9088 SacI G_AGCT'C Cuts at: 6158 6158 Size: 9088 SalI G'TCGA_C Cuts at: 1936 5581 6430 1936 Size: 3645 849 4594 Fragments arranged by size: 4594 3645 849 SanDI GG'GwC_CC Cuts at: 3312 3312 Size: 9088 SapI GCTCTTCn'nnn_ Cuts at: 3881 4872 5390 5937 3881 Size: 991 518 547 7032 Fragments arranged by size: 7032 991 547 518 SbfI CC_TGCA'GG Cuts at: 6440 6440 Size: 9088 ScaI AGT'ACT Cuts at: 6195 7336 9048 6195 Size: 1141 1712 6235 Fragments arranged by size: 6235 1712 1141 SfcI C'TryA_G Cuts at: 826 1045 3000 4042 4204 4682 4789 4901 5514 Size: 219 1955 1042 162 478 107 112 613 Cuts at: 5514 6436 7571 8252 8443 826 Size: 922 1135 681 191 1471 Fragments arranged by size: 1955 1471 1135 1042 922 681 613 478 219 191 162 112 107 SfiI GGCCn_nnn'nGGCC Cuts at: 3968 3968 Size: 9088 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9088 SmaI CCC'GGG Cuts at: 1872 3539 6415 1872 Size: 1667 2876 4545 Fragments arranged by size: 4545 2876 1667 SmlI C'TyrA_G Cuts at: 820 1017 1943 2642 3196 3832 5285 6313 7192 Size: 197 926 699 554 636 1453 1028 879 Cuts at: 7192 8093 8355 820 Size: 901 262 1553 Fragments arranged by size: 1553 1453 1028 926 901 879 699 636 554 262 197 SnaBI TAC'GTA Cuts at: 493 6879 493 Size: 6386 2702 SpeI A'CTAG_T Cuts at: 152 4216 152 Size: 4064 5024 SphI G_CATG'C Cuts at: 1132 5239 6446 1132 Size: 4107 1207 3774 Fragments arranged by size: 4107 3774 1207 SspI AAT'ATT Cuts at: 5 52 7012 5 Size: 47 6960 2081 Fragments arranged by size: 6960 2081 47 StuI AGG'CCT Cuts at: 1517 4141 6067 1517 Size: 2624 1926 4538 Fragments arranged by size: 4538 2624 1926 StyI C'CwwG_G Cuts at: 513 1065 2004 2331 3088 3660 3714 4098 4371 Size: 552 939 327 757 572 54 384 273 Cuts at: 4371 6527 513 Size: 2156 3074 Fragments arranged by size: 3074 2156 939 757 572 552 384 327 273 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3245 4356 4375 4823 5061 5854 6120 Size: 1255 1665 1111 19 448 238 793 266 Cuts at: 6120 7115 7132 7285 7470 7915 325 Size: 995 17 153 185 445 1498 Fragments arranged by size: 1665 1498 1255 1111 995 793 448 445 266 238 185 153 19 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3288 5346 5796 Size: 274 80 33 51 1443 1311 2058 450 Cuts at: 5796 5817 6193 7334 9046 96 Size: 21 376 1141 1712 138 Fragments arranged by size: 2058 1712 1443 1311 1141 450 376 274 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2012 2044 3517 3549 9048 9080 2012 Size: 32 1473 32 5499 32 2020 Fragments arranged by size: 5499 2020 1473 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3694 4307 4385 1316 Size: 2378 613 78 6019 Fragments arranged by size: 6019 2378 613 78 XbaI T'CTAG_A Cuts at: 4480 6424 4480 Size: 1944 7144 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2365 2786 2365 Size: 421 8667 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9088 XmaI C'CCGG_G Cuts at: 1870 3537 6413 1870 Size: 1667 2876 4545 Fragments arranged by size: 4545 2876 1667 XmnI GAAnn'nnTTC Cuts at: 2666 4692 7217 2666 Size: 2026 2525 4537 Fragments arranged by size: 4537 2525 2026 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3852 6896 276 Size: 53 83 186 936 2318 3044 2468 Fragments arranged by size: 3044 2468 2318 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PvuII SacII SexAI SfoI SrfI SwaI