Restriction map for
pFN21AA0709
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# NO CUT ENZYMES ( 73 enzymes) #
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AatII AccIII AclI AflII AflIII
AgeI AocI AscI AseI AsnI
Asp700I AvrII BciVI BclI BfrI
BlnI BsaBI BseAI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eco81I EcoRV EcoT22I FbaI HincII
HindII HpaI KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I RcaI RsrII SacII SalI
SfuI SgrAI SpeI SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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Acc65I 265 GGTACC G^GTACC
AccI 523 GTCTAC GT^[AC][GT]AC
AhdI 382 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 265 GGTACC G^GTACC
AspEI 382 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3536 TGCGCA TGC^GCA
BbvCI 3254 CCTCAGC CCTCAGC(-5/-2)
BcgI 3874 CGACAGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2186 AGATCT A^GATCT
BsmI 3061 GCATTC GAATGC(1/-1)
Eam1105I 382 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2082 GAAGAG CTCTTC(1/4)
EcoRI 1279 GAATTC G^AATTC
FspI 3536 TGCGCA TGC^GCA
HindIII 4180 AAGCTT A^AGCTT
KpnI 265 GGTACC GGTAC^C
Ksp632I 2082 GAAGAG CTCTTC(1/4)
MseI 752 TTAA T^TAA
PvuI 3282 CGATCG CGAT^CG
SacI 3385 GAGCTC GAGCT^C
SapI 2082 GAAGAGC GCTCTTC(1/4)
ScaI 1733 AGTACT AGT^ACT
SexAI 1247 ACCTGGT A^CC[AT]GGT
SnaBI 3664 TACGTA TAC^GTA
SphI 4085 GCATGC GCATG^C
Tru9I 752 TTAA T^TAA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 1279 GAATTC [GA]^AATT[CT]
1353 GAATTT
ApoI 1279 GAATTC [GA]^AATT[CT]
1353 GAATTT
AspI 1246 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1438 GACTGTGTC
BamHI 2253 GGATCC G^GATCC
2927 GGATCC
BbrPI 2532 CACGTG CAC^GTG
2559 CACGTG
BbsI 1609 GAAGAC GAAGAC(2/6)
127 GTCTTC
BfaI 2605 CTAG C^TAG
3197 CTAG
BpuAI 1609 GAAGAC GAAGAC(2/6)
127 GTCTTC
Bsp1407I 1805 TGTACA T^GTACA
2783 TGTACA
BsrGI 1805 TGTACA T^GTACA
2783 TGTACA
BstXI 1114 CCAGACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3261 CCACTTCACTGG
EcoNI 3119 CCTCGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3513 CCTCACGCAGG
FseI 10 GGCCGGCC GGCCGG^CC
14 GGCCGGCC
MaeI 2605 CTAG C^TAG
3197 CTAG
PflFI 1246 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1438 GACTGTGTC
PmaCI 2532 CACGTG CAC^GTG
2559 CACGTG
PmlI 2532 CACGTG CAC^GTG
2559 CACGTG
PpuMI 494 AGGACCT [GA]G^G[AT]CC[CT]
1754 AGGACCT
SbfI 1185 CCTGCAGG CCTGCA^GG
1749 CCTGCAGG
Sse8387I 1185 CCTGCAGG CCTGCA^GG
1749 CCTGCAGG
SspBI 1805 TGTACA T^GTACA
2783 TGTACA
Tth111I 1246 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1438 GACTGTGTC
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# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI