(Circular) (Six-base) MAPSORT of: pFN21AA0709.seq Check: 9262 from: 1 to: 9142 pFN21AA0709 9142 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2055 5964 2055 Size: 3909 5233 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6952 278 Size: 53 83 186 936 5416 2468 Fragments arranged by size: 5416 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2529 6485 1937 Size: 592 3956 4594 Fragments arranged by size: 4594 3956 592 Acc65I G'GTAC_C Cuts at: 2270 6463 2270 Size: 4193 4949 AclI AA'CG_TT Cuts at: 1563 7269 7642 1563 Size: 5706 373 3063 Fragments arranged by size: 5706 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2128 4687 5408 7205 8589 8857 1632 Size: 496 2559 721 1797 1384 268 1917 Fragments arranged by size: 2559 1917 1797 1384 721 496 268 AfeI AGC'GCT Cuts at: 2231 2675 5367 5885 6225 2231 Size: 444 2692 518 340 5148 Fragments arranged by size: 5148 2692 518 444 340 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8945 AflIII A'CryG_T Cuts at: 1203 8041 9018 1203 Size: 6838 977 1327 Fragments arranged by size: 6838 1327 977 AgeI A'CCGG_T Cuts at: 7973 7973 Size: 9142 AhdI GACnn_n'nnGTC Cuts at: 2392 7871 2392 Size: 5479 3663 AleI CACnn'nnGTG Cuts at: 1166 3956 6047 1166 Size: 2790 2091 4261 Fragments arranged by size: 4261 2790 2091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2829 3083 1184 Size: 1645 254 7243 Fragments arranged by size: 7243 1645 254 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4200 4232 4702 4734 4200 Size: 32 470 32 8608 Fragments arranged by size: 8608 470 32 32 AlwNI CAG_nnn'CTG Cuts at: 2284 3489 3946 4123 4138 5797 6885 8457 2284 Size: 1205 457 177 15 1659 1088 1572 2969 Fragments arranged by size: 2969 1659 1572 1205 1088 457 177 15 ApaI G_GGCC'C Cuts at: 2013 2413 3464 3925 4132 4651 5751 6072 6086 Size: 400 1051 461 207 519 1100 321 14 Cuts at: 6086 2013 Size: 5069 Fragments arranged by size: 5069 1100 1051 519 461 400 321 207 14 ApaLI G'TGCA_C Cuts at: 2563 3649 4592 7199 8355 2563 Size: 1086 943 2607 1156 3350 Fragments arranged by size: 3350 2607 1156 1086 943 ApoI r'AATT_y Cuts at: 1493 3284 3358 6451 6677 6829 8938 1493 Size: 1791 74 3093 226 152 2109 1697 Fragments arranged by size: 3093 2109 1791 1697 226 152 74 AseI AT'TA_AT Cuts at: 160 1032 7696 160 Size: 872 6664 1606 Fragments arranged by size: 6664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9142 AvaI C'yCGr_G Cuts at: 1870 1943 2069 3031 3836 4211 4644 5380 5532 Size: 73 126 962 805 375 433 736 152 Cuts at: 5532 6073 6467 1870 Size: 541 394 4545 Fragments arranged by size: 4545 962 805 736 541 433 394 375 152 126 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2254 2287 3832 3865 4434 4467 2254 Size: 33 1545 33 569 33 6929 Fragments arranged by size: 6929 1545 569 33 33 33 BamHI G'GATC_C Cuts at: 4258 4932 6472 6839 4258 Size: 674 1540 367 6561 Fragments arranged by size: 6561 1540 674 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2096 2270 2303 2438 Size: 325 217 627 286 23 174 33 135 Cuts at: 2438 3402 3642 4744 4827 5179 5408 5984 6141 Size: 964 240 1102 83 352 229 576 157 Cuts at: 6141 6318 6350 6463 7918 618 Size: 177 32 113 1455 1842 Fragments arranged by size: 1842 1455 1102 964 627 576 352 325 286 240 229 217 177 174 157 135 113 83 33 32 23 BanII G_rGCy'C Cuts at: 1391 2013 2413 3111 3417 3464 3925 4132 4461 Size: 622 400 698 306 47 461 207 329 Cuts at: 4461 4651 5193 5354 5394 5582 5751 6072 6086 Size: 190 542 161 40 188 169 321 14 Cuts at: 6086 6461 1391 Size: 375 4072 Fragments arranged by size: 4072 698 622 542 461 400 375 329 321 306 207 190 188 169 161 47 40 14 BbeI G_GCGC'C Cuts at: 2100 3646 5988 6145 6322 2100 Size: 1546 2342 157 177 4920 Fragments arranged by size: 4920 2342 1546 177 157 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2125 3621 928 Size: 971 226 1496 6449 Fragments arranged by size: 6449 1496 971 226 BbvCI CC'TCA_GC Cuts at: 5260 5260 Size: 9142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5868 5902 7333 7367 1903 Size: 34 3931 34 1431 34 3678 Fragments arranged by size: 3931 3678 1431 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7034 8244 7034 Size: 1210 7932 BclI T'GATC_A Cuts at: 1548 1548 Size: 9142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2017 2094 3160 Size: 107 122 71 631 605 345 77 1066 Cuts at: 3160 3468 3852 4002 4109 4476 5177 5331 5483 Size: 308 384 150 107 367 701 154 152 Cuts at: 5483 7753 136 Size: 2270 1525 Fragments arranged by size: 2270 1525 1066 701 631 605 384 367 345 308 154 152 150 122 107 107 77 71 BglII A'GATC_T Cuts at: 4191 9137 4191 Size: 4946 4196 BlpI GC'TnA_GC Cuts at: 2825 3533 4139 6095 6198 6559 2825 Size: 708 606 1956 103 361 5408 Fragments arranged by size: 5408 1956 708 606 361 103 Bme1580I G_kGCm'C Cuts at: 1790 2013 2291 2413 2567 3464 3653 3925 3953 Size: 223 278 122 154 897 189 272 28 Cuts at: 3953 4132 4596 4651 4747 5182 5336 5387 5496 Size: 179 464 55 96 435 154 51 109 Cuts at: 5496 5751 6072 6086 6355 7203 8359 1790 Size: 255 321 14 269 848 1156 2573 Fragments arranged by size: 2573 1156 897 848 464 435 321 278 272 269 255 223 189 179 154 154 122 109 96 55 51 28 14 BmgBI CAC'GTC Cuts at: 1318 3582 4450 1318 Size: 2264 868 6010 Fragments arranged by size: 6010 2264 868 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2390 2883 2977 Size: 416 53 83 399 24 973 493 94 Cuts at: 2977 2988 3736 4822 5185 5208 6073 6873 7831 Size: 11 748 1086 363 23 865 800 958 Cuts at: 7831 442 Size: 1753 Fragments arranged by size: 1753 1086 973 958 865 800 748 493 416 399 363 94 83 53 24 23 11 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9142 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4549 4603 5596 4549 Size: 54 993 8095 Fragments arranged by size: 8095 993 54 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 3102 3458 3939 4306 6358 7802 1239 Size: 950 913 356 481 367 2052 1444 2579 Fragments arranged by size: 2579 2052 1444 950 913 481 367 356 Bpu10I CC'TnA_GC Cuts at: 4619 5260 5348 4619 Size: 641 88 8413 Fragments arranged by size: 8413 641 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2465 2939 4458 7267 8132 8430 2465 Size: 474 1519 2809 865 298 3177 Fragments arranged by size: 3177 2809 1519 865 474 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2248 2705 5300 5605 7805 882 Size: 617 749 457 2595 305 2200 2219 Fragments arranged by size: 2595 2219 2200 749 617 457 305 BsaAI yAC'GTr Cuts at: 493 1204 4539 4566 5491 5623 5671 6933 493 Size: 711 3335 27 925 132 48 1262 2702 Fragments arranged by size: 3335 2702 1262 925 711 132 48 27 BsaBI GATnn'nnATC Cuts at: 9136 9136 Size: 9142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 2109 3643 3962 Size: 53 83 186 936 564 12 1534 319 Cuts at: 3962 5985 6142 6319 6949 7331 7966 275 Size: 2023 157 177 630 382 635 1451 Fragments arranged by size: 2023 1534 1451 936 635 630 564 382 319 186 177 157 83 53 12 BsaWI w'CCGG_w Cuts at: 4041 4323 6312 7575 7973 8247 8394 8805 4041 Size: 282 1989 1263 398 274 147 411 4378 Fragments arranged by size: 4378 1989 1263 411 398 282 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3308 3338 3722 3752 4361 Size: 30 165 30 1905 30 384 30 609 Cuts at: 4361 4391 4496 4526 4721 4751 5924 5954 1178 Size: 30 105 30 195 30 1173 30 4366 Fragments arranged by size: 4366 1905 1173 609 384 195 165 105 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2510 2858 3014 3332 3566 4172 4175 Size: 871 453 348 156 318 234 606 3 Cuts at: 4175 5318 5588 5621 5745 1186 Size: 1143 270 33 124 4583 Fragments arranged by size: 4583 1143 871 606 453 348 318 270 234 156 124 33 3 BseYI C'CCAG_C Cuts at: 2226 2676 3607 3992 4020 4092 4123 4131 5031 Size: 450 931 385 28 72 31 8 900 Cuts at: 5031 5280 5359 5860 5992 6086 6238 8345 8841 Size: 249 79 501 132 94 152 2107 496 Cuts at: 8841 2226 Size: 2527 Fragments arranged by size: 2527 2107 931 900 501 496 450 385 249 152 132 94 79 72 31 28 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 2590 4546 4661 5234 5281 1163 Size: 573 327 527 1956 115 573 47 5024 Fragments arranged by size: 5024 1956 573 573 527 327 115 47 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2017 2021 2089 3105 4324 5290 Size: 275 186 16 4 68 1016 1219 966 Cuts at: 5290 6592 7353 8381 1540 Size: 1302 761 1028 2301 Fragments arranged by size: 2301 1302 1219 1028 1016 966 761 275 186 68 16 4 BsiHKAI G_wGCw'C Cuts at: 2512 2567 3653 4167 4530 4596 5394 5832 5955 Size: 55 1086 514 363 66 798 438 123 Cuts at: 5955 7203 7288 8359 2512 Size: 1248 85 1071 3295 Fragments arranged by size: 3295 1248 1086 1071 798 514 438 363 123 85 66 55 BsmI GAATG_Cn' Cuts at: 5066 6653 6746 5066 Size: 1587 93 7462 Fragments arranged by size: 7462 1587 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2291 2413 2512 2567 3111 3417 Size: 399 223 278 122 99 55 544 306 Cuts at: 3417 3464 3653 3925 3953 4132 4167 4461 4530 Size: 47 189 272 28 179 35 294 69 Cuts at: 4530 4596 4651 4747 5182 5193 5336 5354 5387 Size: 66 55 96 435 11 143 18 33 Cuts at: 5387 5394 5496 5582 5751 5832 5955 6072 6086 Size: 7 102 86 169 81 123 117 14 Cuts at: 6086 6355 6461 7203 7288 8359 1391 Size: 269 106 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 544 435 399 306 294 278 272 269 223 189 179 169 143 123 122 117 106 102 99 96 86 85 81 69 66 55 55 47 35 33 28 18 14 11 7 BspEI T'CCGG_A Cuts at: 8805 8805 Size: 9142 BspHI T'CATG_A Cuts at: 7031 8761 7031 Size: 1730 7412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2055 2176 4902 5964 6497 844 Size: 1211 121 2726 1062 533 3489 Fragments arranged by size: 3489 2726 1211 1062 533 121 BsrBI CCG'CTC Cuts at: 1394 1823 2922 4072 5551 5897 7029 1394 Size: 429 1099 1150 1479 346 1132 3507 Fragments arranged by size: 3507 1479 1150 1132 1099 429 346 BsrDI GCAATG_nn' Cuts at: 65 1248 7637 7811 65 Size: 1183 6389 174 1396 Fragments arranged by size: 6389 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2016 2020 2253 3773 3936 4329 7786 Size: 110 206 4 233 1520 163 393 3457 Cuts at: 7786 7973 1700 Size: 187 2869 Fragments arranged by size: 3457 2869 1520 393 233 206 187 163 110 4 BsrGI T'GTAC_A Cuts at: 96 3810 4788 96 Size: 3714 978 4450 Fragments arranged by size: 4450 3714 978 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9142 BssSI C'ACGA_G Cuts at: 2522 2843 2917 4220 6192 7202 8214 2522 Size: 321 74 1303 1972 1010 1012 3450 Fragments arranged by size: 3450 1972 1303 1012 1010 321 74 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9142 BstBI TT'CG_AA Cuts at: 7946 7946 Size: 9142 BstEII G'GTnAC_C Cuts at: 1184 2384 2453 2630 3673 5038 1184 Size: 1200 69 177 1043 1365 5288 Fragments arranged by size: 5288 1365 1200 1043 177 69 BstXI CCAn_nnnn'nTGG Cuts at: 3126 5273 3126 Size: 2147 6995 BstYI r'GATC_y Cuts at: 1972 2818 2838 3982 4191 4215 4258 4422 4431 Size: 846 20 1144 209 24 43 164 9 Cuts at: 4431 4932 6472 6839 7224 7241 8693 8779 8791 Size: 501 1540 367 385 17 1452 86 12 Cuts at: 8791 9137 1972 Size: 346 1977 Fragments arranged by size: 1977 1540 1452 1144 846 501 385 367 346 209 164 86 43 24 20 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 2004 2149 2313 2551 2599 3099 3559 Size: 552 939 145 164 238 48 500 460 Cuts at: 3559 3658 3847 3882 5311 5776 6168 513 Size: 99 189 35 1429 465 392 3487 Fragments arranged by size: 3487 1429 939 552 500 465 460 392 238 189 164 145 99 48 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3046 4707 487 Size: 683 56 1820 1661 4922 Fragments arranged by size: 4922 1820 1661 683 56 BtsI GCAGTG_nn' Cuts at: 2285 2473 2939 3178 4391 4406 5749 5830 6658 Size: 188 466 239 1213 15 1343 81 828 Cuts at: 6658 7452 7472 2285 Size: 794 20 3955 Fragments arranged by size: 3955 1343 1213 828 794 466 239 188 81 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9107 DraI TTT'AAA Cuts at: 6446 6446 Size: 9142 DraIII CAC_nnn'GTG Cuts at: 2384 2892 4171 2384 Size: 508 1279 7355 Fragments arranged by size: 7355 1279 508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8149 809 Size: 507 6833 1802 Fragments arranged by size: 6833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2014 2018 2086 Size: 54 1425 50 275 18 184 4 68 Cuts at: 2086 2297 2470 3102 3867 4146 4173 4392 5275 Size: 211 173 632 765 279 27 219 883 Cuts at: 5275 6589 7478 9107 8 Size: 1314 889 1629 43 Fragments arranged by size: 1629 1425 1314 889 883 765 632 279 275 219 211 184 173 68 54 50 43 27 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2014 2018 2086 3102 6589 1537 Size: 275 202 4 68 1016 3487 4090 Fragments arranged by size: 4090 3487 1016 275 202 68 4 EarI CTCTTCn'nnn_ Cuts at: 1353 4082 7072 1353 Size: 2729 2990 3423 Fragments arranged by size: 3423 2990 2729 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3290 4619 7729 8103 8249 387 Size: 1064 138 1701 1329 3110 374 146 1280 Fragments arranged by size: 3110 1701 1329 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5392 5392 Size: 9142 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2128 2189 3102 3458 3939 4306 4687 Size: 393 496 61 913 356 481 367 381 Cuts at: 4687 5408 6358 7205 7802 8589 8857 1239 Size: 721 950 847 597 787 268 1524 Fragments arranged by size: 1524 950 913 847 787 721 597 496 481 393 381 367 356 268 61 EcoNI CCTnn'n_nnAGG Cuts at: 5128 5522 5128 Size: 394 8748 EcoO109I rG'GnC_Cy Cuts at: 2009 2409 2500 3460 3760 4110 4128 5685 5748 Size: 400 91 960 300 350 18 1557 63 Cuts at: 5748 6068 6069 6083 6339 2009 Size: 320 1 14 256 4812 Fragments arranged by size: 4812 1557 960 400 350 320 300 256 91 63 18 14 1 EcoRI G'AATT_C Cuts at: 3284 6451 6829 3284 Size: 3167 378 5597 Fragments arranged by size: 5597 3167 378 FseI GG_CCGG'CC Cuts at: 2020 2024 2020 Size: 4 9138 FspI TGC'GCA Cuts at: 5543 7648 5543 Size: 2105 7037 HaeII r_GCGC'y Cuts at: 1678 2100 2233 2677 3646 3866 3882 4285 4702 Size: 422 133 444 969 220 16 403 417 Cuts at: 4702 5369 5887 5988 6145 6153 6227 6322 8289 Size: 667 518 101 157 8 74 95 1967 Cuts at: 8289 1678 Size: 2531 Fragments arranged by size: 2531 1967 969 667 518 444 422 417 403 220 157 133 101 95 74 16 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 3647 3679 4361 Size: 32 135 32 806 32 1237 32 682 Cuts at: 4361 4393 7783 7815 7857 7889 1373 Size: 32 3390 32 42 32 2626 Fragments arranged by size: 3390 2626 1237 806 682 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6486 6732 669 Size: 988 281 4548 246 3079 Fragments arranged by size: 4548 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 6185 748 Size: 5437 3705 HpaI GTT'AAC Cuts at: 6732 6732 Size: 9142 KasI G'GCGC_C Cuts at: 2096 3642 5984 6141 6318 2096 Size: 1546 2342 157 177 4920 Fragments arranged by size: 4920 2342 1546 177 157 KpnI G_GTAC'C Cuts at: 2274 6467 2274 Size: 4193 4949 MfeI C'AATT_G Cuts at: 6741 6741 Size: 9142 MluI A'CGCG_T Cuts at: 9018 9018 Size: 9142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2919 3557 6084 8256 8440 1305 Size: 1614 638 2527 2172 184 2007 Fragments arranged by size: 2527 2172 2007 1614 638 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2299 2472 3869 4148 4175 Size: 54 1425 343 467 173 1397 279 27 Cuts at: 4175 4394 10 Size: 219 4758 Fragments arranged by size: 4758 1425 1397 467 343 279 219 173 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 3956 6047 6167 6407 6422 7100 7459 Size: 648 2790 2091 120 240 15 678 359 Cuts at: 7459 7618 518 Size: 159 2042 Fragments arranged by size: 2790 2091 2042 678 648 359 240 159 120 15 MspA1I CmG'CkG Cuts at: 1606 2392 2734 2745 2799 3210 3474 3532 3670 Size: 786 342 11 54 411 264 58 138 Cuts at: 3670 4135 4414 5035 5235 5280 5434 5614 5703 Size: 465 279 621 200 45 154 180 89 Cuts at: 5703 5784 6047 6108 6132 6242 6558 7235 8383 Size: 81 263 61 24 110 316 677 1148 Cuts at: 8383 8628 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 786 677 621 465 411 342 316 279 264 263 245 200 180 154 138 110 89 81 61 58 54 45 24 11 NaeI GCC'GGC Cuts at: 2018 2022 3938 4331 2018 Size: 4 1916 393 6829 Fragments arranged by size: 6829 1916 393 4 NarI GG'CG_CC Cuts at: 2097 3643 5985 6142 6319 2097 Size: 1546 2342 157 177 4920 Fragments arranged by size: 4920 2342 1546 177 157 NcoI C'CATG_G Cuts at: 513 1065 2004 2149 3559 3658 3882 5311 5776 Size: 552 939 145 1410 99 224 1429 465 Cuts at: 5776 6168 513 Size: 392 3487 Fragments arranged by size: 3487 1429 1410 939 552 465 392 224 145 99 NdeI CA'TA_TG Cuts at: 387 387 Size: 9142 NgoMIV G'CCGG_C Cuts at: 2016 2020 3936 4329 2016 Size: 4 1916 393 6829 Fragments arranged by size: 6829 1916 393 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9142 NotI GC'GGCC_GC Cuts at: 6589 6589 Size: 9142 NspI r_CATG'y Cuts at: 1132 1229 2332 3234 4183 6094 6500 8045 1132 Size: 97 1103 902 949 1911 406 1545 2229 Fragments arranged by size: 2229 1911 1545 1103 949 902 406 97 PasI CC'CwG_GG Cuts at: 1348 2102 2176 2323 4015 4105 4909 5791 1348 Size: 754 74 147 1692 90 804 882 4699 Fragments arranged by size: 4699 1692 882 804 754 147 90 74 PciI A'CATG_T Cuts at: 8041 8041 Size: 9142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2892 3271 4123 5146 5797 1108 Size: 1784 379 852 1023 651 4453 Fragments arranged by size: 4453 1784 1023 852 651 379 PfoI T'CCnGG_A Cuts at: 2118 3426 4500 5823 5919 2118 Size: 1308 1074 1323 96 5341 Fragments arranged by size: 5341 1323 1308 1074 96 PmeI GTTT'AAAC Cuts at: 6446 6446 Size: 9142 PmlI CAC'GTG Cuts at: 4539 4566 4539 Size: 27 9115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 4502 4534 4991 5023 7211 7243 8724 Size: 32 574 32 457 32 2188 32 1481 Cuts at: 8724 8756 3896 Size: 32 4282 Fragments arranged by size: 4282 2188 1481 574 457 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2500 3760 2500 Size: 1260 7882 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9142 PsiI TTA'TAA Cuts at: 4727 6712 4727 Size: 1985 7157 PspOMI G'GGCC_C Cuts at: 2009 2409 3460 3921 4128 4647 5747 6068 6082 Size: 400 1051 461 207 519 1100 321 14 Cuts at: 6082 2009 Size: 5069 Fragments arranged by size: 5069 1100 1051 519 461 400 321 207 14 PstI C_TGCA'G Cuts at: 830 2160 3059 3195 3354 3759 4080 4386 5721 Size: 1330 899 136 159 405 321 306 1335 Cuts at: 5721 5961 6494 830 Size: 240 533 3478 Fragments arranged by size: 3478 1335 1330 899 533 405 321 306 240 159 136 PvuI CG_AT'CG Cuts at: 2001 5290 2001 Size: 3289 5853 PvuII CAG'CTG Cuts at: 2392 2734 2745 2799 3210 3532 3670 4135 4414 Size: 342 11 54 411 322 138 465 279 Cuts at: 4414 5035 5235 5703 5784 6047 6108 2392 Size: 621 200 468 81 263 61 5426 Fragments arranged by size: 5426 621 468 465 411 342 322 279 263 200 138 81 61 54 11 SacI G_AGCT'C Cuts at: 5394 5394 Size: 9142 SalI G'TCGA_C Cuts at: 1936 6484 1936 Size: 4548 4594 SapI GCTCTTCn'nnn_ Cuts at: 4082 4082 Size: 9142 SbfI CC_TGCA'GG Cuts at: 3195 3759 6494 3195 Size: 564 2735 5843 Fragments arranged by size: 5843 2735 564 ScaI AGT'ACT Cuts at: 3740 7390 9102 3740 Size: 3650 1712 3780 Fragments arranged by size: 3780 3650 1712 SexAI A'CCwGG_T Cuts at: 3252 3252 Size: 9142 SfcI C'TryA_G Cuts at: 826 1045 2156 3055 3191 3350 3628 3755 4076 Size: 219 1111 899 136 159 278 127 321 Cuts at: 4076 4382 4902 5717 5957 6358 6490 7625 8306 Size: 306 520 815 240 401 132 1135 681 Cuts at: 8306 8497 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1111 899 815 681 520 401 321 306 278 240 219 191 159 136 132 127 SfiI GGCCn_nnn'nGGCC Cuts at: 2017 3468 4109 2017 Size: 1451 641 7050 Fragments arranged by size: 7050 1451 641 SfoI GGC'GCC Cuts at: 2098 3644 5986 6143 6320 2098 Size: 1546 2342 157 177 4920 Fragments arranged by size: 4920 2342 1546 177 157 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9142 SmaI CCC'GGG Cuts at: 1872 3838 4646 5382 6075 6469 1872 Size: 1966 808 736 693 394 4545 Fragments arranged by size: 4545 1966 808 736 693 394 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 2954 4473 7246 8147 8409 Size: 197 926 501 510 1519 2773 901 262 Cuts at: 8409 820 Size: 1553 Fragments arranged by size: 2773 1553 1519 926 901 510 501 262 197 SnaBI TAC'GTA Cuts at: 493 5671 6933 493 Size: 5178 1262 2702 Fragments arranged by size: 5178 2702 1262 SpeI A'CTAG_T Cuts at: 152 152 Size: 9142 SphI G_CATG'C Cuts at: 1132 6094 6500 1132 Size: 4962 406 3774 Fragments arranged by size: 4962 3774 406 SspI AAT'ATT Cuts at: 5 52 7066 5 Size: 47 7014 2081 Fragments arranged by size: 7014 2081 47 StuI AGG'CCT Cuts at: 1517 2789 3701 5513 1517 Size: 1272 912 1812 5146 Fragments arranged by size: 5146 1812 1272 912 StyI C'CwwG_G Cuts at: 513 1065 2004 2149 3559 3658 3882 5311 5776 Size: 552 939 145 1410 99 224 1429 465 Cuts at: 5776 6168 6581 513 Size: 392 413 3074 Fragments arranged by size: 3074 1429 1410 939 552 465 413 392 224 145 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2372 2666 3410 3830 4378 5134 5522 Size: 1255 792 294 744 420 548 756 388 Cuts at: 5522 6161 7169 7186 7339 7524 7969 325 Size: 639 1008 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1008 792 756 744 639 548 445 420 388 294 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3738 3810 4512 Size: 274 80 33 51 1443 1761 72 702 Cuts at: 4512 4788 7388 9100 96 Size: 276 2600 1712 138 Fragments arranged by size: 2600 1761 1712 1443 702 276 274 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3308 3340 3578 3610 4766 4798 4811 4843 5126 Size: 32 238 32 1156 32 13 32 283 Cuts at: 5126 5158 9102 9134 3308 Size: 32 3944 32 3316 Fragments arranged by size: 3944 3316 1156 283 238 32 32 32 32 32 32 13 Tth111I GACn'n_nGTC Cuts at: 1316 3254 3446 1316 Size: 1938 192 7012 Fragments arranged by size: 7012 1938 192 XbaI T'CTAG_A Cuts at: 6478 6478 Size: 9142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2281 2320 2465 3139 3600 5239 5897 6166 2281 Size: 39 145 674 461 1639 658 269 5257 Fragments arranged by size: 5257 1639 674 658 461 269 145 39 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9142 XmaI C'CCGG_G Cuts at: 1870 3836 4644 5380 6073 6467 1870 Size: 1966 808 736 693 394 4545 Fragments arranged by size: 4545 1966 808 736 693 394 XmnI GAAnn'nnTTC Cuts at: 7271 7271 Size: 9142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6950 276 Size: 53 83 186 936 5416 2468 Fragments arranged by size: 5416 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SrfI SwaI