(Circular) (Six-base) MAPSORT of: pFN21AA0440.seq Check: 9610 from: 1 to: 10114 pFN21AA0440 10114 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7323 7323 Size: 10114 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6843 7924 278 Size: 53 83 186 936 5307 1081 2468 Fragments arranged by size: 5307 2468 1081 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 3677 7457 1937 Size: 1413 327 3780 4594 Fragments arranged by size: 4594 3780 1413 327 Acc65I G'GTAC_C Cuts at: 7435 7435 Size: 10114 AclI AA'CG_TT Cuts at: 1563 8241 8614 1563 Size: 6678 373 3063 Fragments arranged by size: 6678 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2493 3897 4620 6229 6832 7265 7280 7391 Size: 861 1404 723 1609 603 433 15 111 Cuts at: 7391 8177 9561 9829 1632 Size: 786 1384 268 1917 Fragments arranged by size: 1917 1609 1404 1384 861 786 723 603 433 268 111 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9917 AflIII A'CryG_T Cuts at: 1203 2981 3454 9013 9990 1203 Size: 1778 473 5559 977 1327 Fragments arranged by size: 5559 1778 1327 977 473 AgeI A'CCGG_T Cuts at: 8945 8945 Size: 10114 AhdI GACnn_n'nnGTC Cuts at: 8843 8843 Size: 10114 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10114 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2438 2470 3933 3965 2438 Size: 32 1463 32 8587 Fragments arranged by size: 8587 1463 32 32 AlwNI CAG_nnn'CTG Cuts at: 2976 3548 3760 4105 4251 7165 7315 7857 9429 Size: 572 212 345 146 2914 150 542 1572 Cuts at: 9429 2976 Size: 3661 Fragments arranged by size: 3661 2914 1572 572 542 345 212 150 146 ApaLI G'TGCA_C Cuts at: 4226 8171 9327 4226 Size: 3945 1156 5013 Fragments arranged by size: 5013 3945 1156 ApoI r'AATT_y Cuts at: 1493 3181 3307 3485 3923 5174 7423 7649 7801 Size: 1688 126 178 438 1251 2249 226 152 Cuts at: 7801 9910 1493 Size: 2109 1697 Fragments arranged by size: 2249 2109 1697 1688 1251 438 226 178 152 126 AseI AT'TA_AT Cuts at: 160 1032 4371 5783 8668 160 Size: 872 3339 1412 2885 1606 Fragments arranged by size: 3339 2885 1606 1412 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10114 AvaI C'yCGr_G Cuts at: 1870 1943 2491 5693 5848 7439 1870 Size: 73 548 3202 155 1591 4545 Fragments arranged by size: 4545 3202 1591 548 155 73 AvrII C'CTAG_G Cuts at: 6413 6413 Size: 10114 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6259 6292 6259 Size: 33 10081 BamHI G'GATC_C Cuts at: 5794 7444 7811 5794 Size: 1650 367 8097 Fragments arranged by size: 8097 1650 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 5361 5966 6082 7435 Size: 325 217 627 291 3283 605 116 1353 Cuts at: 7435 8890 618 Size: 1455 1842 Fragments arranged by size: 3283 1842 1455 1353 627 605 325 291 217 116 BanII G_rGCy'C Cuts at: 1391 3636 3747 4563 4941 5771 6287 7433 1391 Size: 2245 111 816 378 830 516 1146 4072 Fragments arranged by size: 4072 2245 1146 830 816 516 378 111 BbeI G_GCGC'C Cuts at: 5365 5365 Size: 10114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2967 4608 4684 5457 6165 7287 7384 Size: 971 1068 1641 76 773 708 1122 97 Cuts at: 7384 928 Size: 3658 Fragments arranged by size: 3658 1641 1122 1068 971 773 708 97 76 BbvCI CC'TCA_GC Cuts at: 3197 4967 3197 Size: 1770 8344 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4961 4995 8305 8339 1903 Size: 34 3024 34 3310 34 3678 Fragments arranged by size: 3678 3310 3024 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8006 9216 8006 Size: 1210 8904 BclI T'GATC_A Cuts at: 1548 7144 1548 Size: 5596 4518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4958 5614 6210 Size: 107 122 71 631 605 3286 656 596 Cuts at: 6210 8725 136 Size: 2515 1525 Fragments arranged by size: 3286 2515 1525 656 631 605 596 122 107 71 BglII A'GATC_T Cuts at: 2938 3460 4447 4992 6622 10109 2938 Size: 522 987 545 1630 3487 2943 Fragments arranged by size: 3487 2943 1630 987 545 522 BlpI GC'TnA_GC Cuts at: 4562 7531 4562 Size: 2969 7145 Bme1580I G_kGCm'C Cuts at: 1790 3402 4230 5498 5896 6325 8175 9331 1790 Size: 1612 828 1268 398 429 1850 1156 2573 Fragments arranged by size: 2573 1850 1612 1268 1156 828 429 398 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10114 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5431 5569 5766 Size: 416 53 83 399 24 4014 138 197 Cuts at: 5766 6299 7845 8803 442 Size: 533 1546 958 1753 Fragments arranged by size: 4014 1753 1546 958 533 416 399 197 138 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10114 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2517 2834 3971 4577 2517 Size: 317 1137 606 8054 Fragments arranged by size: 8054 1137 606 317 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2440 2679 2895 3108 3680 3746 4179 5301 Size: 1201 239 216 213 572 66 433 1122 Cuts at: 5301 5335 5842 5860 6085 6787 7172 8774 1239 Size: 34 507 18 225 702 385 1602 2579 Fragments arranged by size: 2579 1602 1201 1122 702 572 507 433 385 239 225 216 213 66 34 18 Bpu10I CC'TnA_GC Cuts at: 2140 3197 3741 4967 6058 6179 6591 2140 Size: 1057 544 1226 1091 121 412 5663 Fragments arranged by size: 5663 1226 1091 1057 544 412 121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2296 2834 5226 7144 8239 9104 9402 2296 Size: 538 2392 1918 1095 865 298 3008 Fragments arranged by size: 3008 2392 1918 1095 865 538 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3124 5416 6567 6965 8777 882 Size: 617 1625 2292 1151 398 1812 2219 Fragments arranged by size: 2292 2219 1812 1625 1151 617 398 BsaAI yAC'GTr Cuts at: 493 1204 3457 3733 6858 7905 493 Size: 711 2253 276 3125 1047 2702 Fragments arranged by size: 3125 2702 2253 1047 711 276 BsaBI GATnn'nnATC Cuts at: 3531 10108 3531 Size: 6577 3537 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3666 5362 6809 6840 Size: 53 83 186 936 2133 1696 1447 31 Cuts at: 6840 7921 8303 8938 275 Size: 1081 382 635 1451 Fragments arranged by size: 2133 1696 1451 1447 1081 936 635 382 186 83 53 31 BsaWI w'CCGG_w Cuts at: 2121 6159 8547 8945 9219 9366 9777 2121 Size: 4038 2388 398 274 147 411 2458 Fragments arranged by size: 4038 2458 2388 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 2471 2501 3372 Size: 30 165 30 1035 30 3 30 871 Cuts at: 3372 3402 3933 3963 4086 4116 4758 4788 6677 Size: 30 531 30 123 30 642 30 1889 Cuts at: 6677 6707 1178 Size: 30 4585 Fragments arranged by size: 4585 1889 1035 871 642 531 165 123 30 30 30 30 30 30 30 30 30 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2872 3085 4779 4817 5122 5304 6376 6379 Size: 1686 213 1694 38 305 182 1072 3 Cuts at: 6379 6388 6391 6394 6685 6690 6904 7189 7358 Size: 9 3 3 291 5 214 285 169 Cuts at: 7358 1186 Size: 3942 Fragments arranged by size: 3942 1694 1686 1072 305 291 285 214 213 182 169 38 9 5 3 3 3 BseYI C'CCAG_C Cuts at: 2483 3910 5548 5731 6008 6031 6716 9317 9813 Size: 1427 1638 183 277 23 685 2601 496 Cuts at: 9813 2483 Size: 2784 Fragments arranged by size: 2784 2601 1638 1427 685 496 277 183 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2594 5080 5279 7328 1163 Size: 573 858 2486 199 2049 3949 Fragments arranged by size: 3949 2486 2049 858 573 199 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7564 8325 9353 1540 Size: 275 186 5563 761 1028 2301 Fragments arranged by size: 5563 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3636 3732 3747 3875 3982 4165 4230 4241 4563 Size: 96 15 128 107 183 65 11 322 Cuts at: 4563 4665 6287 6792 8175 8260 9331 3636 Size: 102 1622 505 1383 85 1071 4419 Fragments arranged by size: 4419 1622 1383 1071 505 322 183 128 107 102 96 85 65 15 11 BsmI GAATG_Cn' Cuts at: 3555 5290 5830 7625 7718 3555 Size: 1735 540 1795 93 5951 Fragments arranged by size: 5951 1795 1735 540 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3402 3636 3732 3747 3875 3982 4165 Size: 399 1612 234 96 15 128 107 183 Cuts at: 4165 4230 4241 4563 4665 4941 5498 5771 5896 Size: 65 11 322 102 276 557 273 125 Cuts at: 5896 6287 6325 6792 7433 8175 8260 9331 1391 Size: 391 38 467 641 742 85 1071 2174 Fragments arranged by size: 2174 1612 1071 742 641 557 467 399 391 322 276 273 234 183 128 125 107 102 96 85 65 38 15 11 BspEI T'CCGG_A Cuts at: 9777 9777 Size: 10114 BspHI T'CATG_A Cuts at: 3636 3801 8003 9733 3636 Size: 165 4202 1730 4017 Fragments arranged by size: 4202 4017 1730 165 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 7323 7469 844 Size: 6479 146 3489 Fragments arranged by size: 6479 3489 146 BsrBI CCG'CTC Cuts at: 1394 1823 8001 1394 Size: 429 6178 3507 Fragments arranged by size: 6178 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2911 3232 3364 5634 5887 6541 8609 Size: 1183 1663 321 132 2270 253 654 2068 Cuts at: 8609 8783 65 Size: 174 1396 Fragments arranged by size: 2270 2068 1663 1396 1183 654 321 253 174 132 BsrFI r'CCGG_y Cuts at: 1700 1810 6209 8758 8945 1700 Size: 110 4399 2549 187 2869 Fragments arranged by size: 4399 2869 2549 187 110 BsrGI T'GTAC_A Cuts at: 96 3888 4023 96 Size: 3792 135 6187 Fragments arranged by size: 6187 3792 135 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10114 BssSI C'ACGA_G Cuts at: 6508 8174 9186 6508 Size: 1666 1012 7436 Fragments arranged by size: 7436 1666 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2243 4382 6988 7315 794 Size: 1449 2139 2606 327 3593 Fragments arranged by size: 3593 2606 2139 1449 327 BstBI TT'CG_AA Cuts at: 3559 8918 3559 Size: 5359 4755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10114 BstXI CCAn_nnnn'nTGG Cuts at: 4157 6307 4157 Size: 2150 7964 BstYI r'GATC_y Cuts at: 1972 2938 3460 3593 4447 4641 4992 5432 5543 Size: 966 522 133 854 194 351 440 111 Cuts at: 5543 5794 6622 7444 7811 8196 8213 9665 9751 Size: 251 828 822 367 385 17 1452 86 Cuts at: 9751 9763 10109 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 966 854 828 822 522 440 385 367 351 346 251 194 133 111 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2819 4106 5670 6907 7331 2819 Size: 1287 1564 1237 424 5602 Fragments arranged by size: 5602 1564 1287 1237 424 BtgI C'CryG_G Cuts at: 513 1065 3105 4413 4617 5102 5418 6013 6117 Size: 552 2040 1308 204 485 316 595 104 Cuts at: 6117 6148 6492 513 Size: 31 344 4135 Fragments arranged by size: 4135 2040 1308 595 552 485 344 316 204 104 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6543 7100 487 Size: 683 56 5317 557 3501 Fragments arranged by size: 5317 3501 683 557 56 BtsI GCAGTG_nn' Cuts at: 2606 7020 7630 8424 8444 2606 Size: 4414 610 794 20 4276 Fragments arranged by size: 4414 4276 794 610 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4668 4703 529 Size: 35 4104 35 5940 Fragments arranged by size: 5940 4104 35 35 DraI TTT'AAA Cuts at: 7418 7418 Size: 10114 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2437 9121 809 Size: 507 1121 6684 1802 Fragments arranged by size: 6684 1802 1121 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 7561 8450 10079 Size: 54 1425 50 275 18 5731 889 1629 Cuts at: 10079 8 Size: 43 Fragments arranged by size: 5731 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7561 1537 Size: 275 5749 4090 Fragments arranged by size: 5749 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2508 3876 6336 6397 6665 6851 8044 1353 Size: 1155 1368 2460 61 268 186 1193 3423 Fragments arranged by size: 3423 2460 1368 1193 1155 268 186 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4943 7316 8701 9075 9221 387 Size: 1064 138 3354 2373 1385 374 146 1280 Fragments arranged by size: 3354 2373 1385 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3634 3745 4561 6285 3634 Size: 111 816 1724 7463 Fragments arranged by size: 7463 1724 816 111 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2440 2493 2679 2895 3108 3680 3746 Size: 393 808 53 186 216 213 572 66 Cuts at: 3746 3897 4179 4620 5301 5335 5842 5860 6085 Size: 151 282 441 681 34 507 18 225 Cuts at: 6085 6229 6787 6832 7172 7265 7280 7391 8177 Size: 144 558 45 340 93 15 111 786 Cuts at: 8177 8774 9561 9829 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 808 787 786 681 597 572 558 507 441 393 340 282 268 225 216 213 186 151 144 111 93 66 53 45 34 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 6057 7319 6057 Size: 1262 8852 EcoO109I rG'GnC_Cy Cuts at: 2055 2168 2973 3175 5228 5666 2055 Size: 113 805 202 2053 438 6503 Fragments arranged by size: 6503 2053 805 438 202 113 EcoRI G'AATT_C Cuts at: 3181 3923 5174 7423 7801 3181 Size: 742 1251 2249 378 5494 Fragments arranged by size: 5494 2249 1251 742 378 EcoRV GAT'ATC Cuts at: 2536 4132 2536 Size: 1596 8518 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3427 3944 5203 6362 7310 3427 Size: 517 1259 1159 948 6231 Fragments arranged by size: 6231 1259 1159 948 517 FspI TGC'GCA Cuts at: 2903 8620 2903 Size: 5717 4397 HaeII r_GCGC'y Cuts at: 1678 2119 5365 6441 9261 1678 Size: 441 3246 1076 2820 2531 Fragments arranged by size: 3246 2820 2531 1076 441 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2315 2347 2687 Size: 32 135 32 470 32 241 32 340 Cuts at: 2687 2719 2939 2971 3639 3671 5299 5331 5981 Size: 32 220 32 668 32 1628 32 650 Cuts at: 5981 6013 6502 6534 8755 8787 8829 8861 1373 Size: 32 489 32 2221 32 42 32 2626 Fragments arranged by size: 2626 2221 1628 668 650 489 470 340 241 220 135 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3678 4909 7458 7704 669 Size: 988 281 1740 1231 2549 246 3079 Fragments arranged by size: 3079 2549 1740 1231 988 281 246 HindIII A'AGCT_T Cuts at: 748 2665 748 Size: 1917 8197 HpaI GTT'AAC Cuts at: 7704 7704 Size: 10114 KasI G'GCGC_C Cuts at: 5361 5361 Size: 10114 KpnI G_GTAC'C Cuts at: 7439 7439 Size: 10114 MfeI C'AATT_G Cuts at: 2637 4140 4696 7713 2637 Size: 1503 556 3017 5038 Fragments arranged by size: 5038 3017 1503 556 MluI A'CGCG_T Cuts at: 9990 9990 Size: 10114 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 2741 2747 5423 6738 7068 7161 9228 Size: 1116 320 6 2676 1315 330 93 2067 Cuts at: 9228 9412 1305 Size: 184 2007 Fragments arranged by size: 2676 2067 2007 1315 1116 330 320 184 93 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 8292 Fragments arranged by size: 8292 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2097 3006 5135 5402 5901 6088 7406 Size: 648 931 909 2129 267 499 187 1318 Cuts at: 7406 8072 8431 8590 518 Size: 666 359 159 2042 Fragments arranged by size: 2129 2042 1318 931 909 666 648 499 359 267 187 159 MspA1I CmG'CkG Cuts at: 1606 3101 3905 5104 5595 6356 7150 7231 7530 Size: 1495 804 1199 491 761 794 81 299 Cuts at: 7530 8207 9355 9600 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1495 1199 1148 804 794 761 677 491 299 245 81 NaeI GCC'GGC Cuts at: 6211 6211 Size: 10114 NarI GG'CG_CC Cuts at: 5362 5362 Size: 10114 NcoI C'CATG_G Cuts at: 513 1065 4413 4617 5418 6117 513 Size: 552 3348 204 801 699 4510 Fragments arranged by size: 4510 3348 801 699 552 204 NdeI CA'TA_TG Cuts at: 387 387 Size: 10114 NgoMIV G'CCGG_C Cuts at: 6209 6209 Size: 10114 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10114 NotI GC'GGCC_GC Cuts at: 7561 7561 Size: 10114 NspI r_CATG'y Cuts at: 1132 1229 2113 2985 5134 5724 7472 9017 1132 Size: 97 884 872 2149 590 1748 1545 2229 Fragments arranged by size: 2229 2149 1748 1545 884 872 590 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10114 PciI A'CATG_T Cuts at: 2981 9013 2981 Size: 6032 4082 PflMI CCAn_nnn'nTGG Cuts at: 1108 4584 6154 1108 Size: 3476 1570 5068 Fragments arranged by size: 5068 3476 1570 PfoI T'CCnGG_A Cuts at: 3657 4549 6966 3657 Size: 892 2417 6805 Fragments arranged by size: 6805 2417 892 PmeI GTTT'AAAC Cuts at: 7418 7418 Size: 10114 PmlI CAC'GTG Cuts at: 3733 3733 Size: 10114 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2438 2470 3933 3965 4003 4035 8183 8215 9696 Size: 32 1463 32 38 32 4148 32 1481 Cuts at: 9696 9728 2438 Size: 32 2824 Fragments arranged by size: 4148 2824 1481 1463 38 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2168 3175 5666 2168 Size: 1007 2491 6616 Fragments arranged by size: 6616 2491 1007 PshAI GACnn'nnGTC Cuts at: 1613 3130 1613 Size: 1517 8597 PsiI TTA'TAA Cuts at: 7684 7684 Size: 10114 PspXI vC'TCGA_Gb Cuts at: 5693 5848 5693 Size: 155 9959 PstI C_TGCA'G Cuts at: 830 7030 7466 830 Size: 6200 436 3478 Fragments arranged by size: 6200 3478 436 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10114 PvuII CAG'CTG Cuts at: 3101 3905 6356 7150 7231 3101 Size: 804 2451 794 81 5984 Fragments arranged by size: 5984 2451 804 794 81 SacI G_AGCT'C Cuts at: 3636 3747 4563 6287 3636 Size: 111 816 1724 7463 Fragments arranged by size: 7463 1724 816 111 SacII CC_GC'GG Cuts at: 5105 5105 Size: 10114 SalI G'TCGA_C Cuts at: 1936 3676 7456 1936 Size: 1740 3780 4594 Fragments arranged by size: 4594 3780 1740 SapI GCTCTTCn'nnn_ Cuts at: 2508 6336 2508 Size: 3828 6286 SbfI CC_TGCA'GG Cuts at: 7466 7466 Size: 10114 ScaI AGT'ACT Cuts at: 3390 8362 10074 3390 Size: 4972 1712 3430 Fragments arranged by size: 4972 3430 1712 SexAI A'CCwGG_T Cuts at: 4271 7047 4271 Size: 2776 7338 SfcI C'TryA_G Cuts at: 826 1045 2163 4705 7026 7337 7462 8597 9278 Size: 219 1118 2542 2321 311 125 1135 681 Cuts at: 9278 9469 826 Size: 191 1471 Fragments arranged by size: 2542 2321 1471 1135 1118 681 311 219 191 125 SfoI GGC'GCC Cuts at: 5363 5363 Size: 10114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10114 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10114 SmaI CCC'GGG Cuts at: 1872 7441 1872 Size: 5569 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2311 2849 5241 5693 5848 7123 Size: 197 926 368 538 2392 452 155 1275 Cuts at: 7123 8218 9119 9381 820 Size: 1095 901 262 1553 Fragments arranged by size: 2392 1553 1275 1095 926 901 538 452 368 262 197 155 SnaBI TAC'GTA Cuts at: 493 7905 493 Size: 7412 2702 SpeI A'CTAG_T Cuts at: 152 3626 152 Size: 3474 6640 SphI G_CATG'C Cuts at: 1132 5134 7472 1132 Size: 4002 2338 3774 Fragments arranged by size: 4002 3774 2338 SspI AAT'ATT Cuts at: 5 52 8038 5 Size: 47 7986 2081 Fragments arranged by size: 7986 2081 47 StuI AGG'CCT Cuts at: 1517 2038 3705 6895 1517 Size: 521 1667 3190 4736 Fragments arranged by size: 4736 3190 1667 521 StyI C'CwwG_G Cuts at: 513 1065 3114 3402 3783 4413 4617 5418 6117 Size: 552 2049 288 381 630 204 801 699 Cuts at: 6117 6413 7553 513 Size: 296 1140 3074 Fragments arranged by size: 3074 2049 1140 801 699 630 552 381 296 288 204 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3455 3643 3796 4099 5588 8141 8158 Size: 1255 1875 188 153 303 1489 2553 17 Cuts at: 8158 8311 8496 8941 325 Size: 153 185 445 1498 Fragments arranged by size: 2553 1875 1498 1489 1255 445 303 188 185 153 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3388 3852 3888 Size: 274 80 33 51 1443 1411 464 36 Cuts at: 3888 4023 5145 8360 10072 96 Size: 135 1122 3215 1712 138 Fragments arranged by size: 3215 1712 1443 1411 1122 464 274 138 135 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5229 5261 6677 6709 10074 10106 5229 Size: 32 1416 32 3365 32 5237 Fragments arranged by size: 5237 3365 1416 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2966 4247 1316 Size: 1650 1281 7183 Fragments arranged by size: 7183 1650 1281 XbaI T'CTAG_A Cuts at: 7450 7450 Size: 10114 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3409 5582 6490 3409 Size: 2173 908 7033 Fragments arranged by size: 7033 2173 908 XhoI C'TCGA_G Cuts at: 1943 5693 5848 1943 Size: 3750 155 6209 Fragments arranged by size: 6209 3750 155 XmaI C'CCGG_G Cuts at: 1870 7439 1870 Size: 5569 4545 XmnI GAAnn'nnTTC Cuts at: 3927 4015 6345 7376 8243 3927 Size: 88 2330 1031 867 5798 Fragments arranged by size: 5798 2330 1031 867 88 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6841 7922 276 Size: 53 83 186 936 5307 1081 2468 Fragments arranged by size: 5307 2468 1081 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BfrBI BsiWI BsmBI BstZ17I DraIII FseI FspAI NruI NsiI PacI PspOMI PsrI RsrII SanDI SfiI SrfI SwaI