Restriction map for
pFN21AA0335
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AspEI
AviII BalI BamHI BbeI BbrPI
BbvCI BcgI BclI BcnI BglI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspMI BsrBI BsrFI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI FauI
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KspI MamI MflI
MluI MluNI MroI MscI MspI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 1774 GGTACC G^GTACC
AlwI 1903 GGATC GGATC(4/5)
Asp718I 1774 GGTACC G^GTACC
AspI 2667 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 2067 GAGCTC G[GA]GC[CT]^C
BpmI 2308 CTCCAG CTGGAG(16/14)
BsmFI 1218 GGGAC GGGAC(10/14)
BspHI 1241 TCATGA T^CATGA
BstBI 2487 TTCGAA TT^CGAA
BstXI 648 CCAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 1904 GATC ^GATC
DraIII 599 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 429 CCATGG C^C[GA][CT]GG
EcoRV 1599 GATATC GAT^ATC
HaeIII 3145 GGCC GG^CC
HgaI 4060 GACGC GACGC(5/10)
KpnI 1774 GGTACC GGTAC^C
MboI 1904 GATC ^GATC
MfeI 2353 CAATTG C^AATTG
MunI 2353 CAATTG C^AATTG
NcoI 429 CCATGG C^CATGG
NdeII 1904 GATC ^GATC
NspV 2487 TTCGAA TT^CGAA
PflFI 2667 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1260 AGGACCT [GA]G^G[AT]CC[CT]
PstI 923 CTGCAG CTGCA^G
PvuII 357 CAGCTG CAG^CTG
RcaI 1241 TCATGA T^CATGA
SacI 2067 GAGCTC GAGCT^C
Sau3AI 1904 GATC ^GATC
ScaI 3376 AGTACT AGT^ACT
SfcI 923 CTGCAG C^T[GA][CT]AG
SfuI 2487 TTCGAA TT^CGAA
SpeI 2052 ACTAGT A^CTAGT
Tth111I 2667 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AciI 2972 CCGC CCGC(-3/-1)
3981 GCGG
AseI 1858 ATTAAT AT^TAAT
2542 ATTAAT
AsnI 1858 ATTAAT AT^TAAT
2542 ATTAAT
Asp700I 972 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2490 GAAAGATTTC
AspHI 2067 GAGCTC G[AT]GC[AT]^C
3216 GAGCAC
AvaI 1762 CCCGAG C^[CT]CG[GA]G
4427 CTCGGG
AvaII 1219 GGACC G^G[AT]CC
1261 GGACC
AvrII 3949 CCTAGG C^CTAGG
4021 CCTAGG
BciVI 4383 GGATAC GTATCC(6/5)
2894 GTATCC
BlnI 3949 CCTAGG C^CTAGG
4021 CCTAGG
BsiHKAI 2067 GAGCTC G[AT]GC[AT]^C
3216 GAGCAC
BsmAI 374 GAGAC GTCTC(1/5)
4407 GAGAC
BsoBI 1762 CCCGAG C^[CT]CG[GA]G
4427 CTCGGG
Bsp1407I 286 TGTACA T^GTACA
2198 TGTACA
BsrGI 286 TGTACA T^GTACA
2198 TGTACA
BssSI 2458 CACGAG CACGAG(-5/-1)
2999 CTCGTG
Cac8I 2423 GCTTGC GC[ACGT]^[ACGT]GC
3603 GCCAGC
DraII 1260 AGGACCT [GA]G^G[ACGT]CC[CT]
3144 AGGCCCT
EarI 319 GAAGAG CTCTTC(1/4)
1213 GAAGAG
EcoO109I 1260 AGGACCT [GA]G^G[ACGT]CC[CT]
3144 AGGCCCT
EcoT22I 1823 ATGCAT ATGCA^T
1843 ATGCAT
Ksp632I 319 GAAGAG CTCTTC(1/4)
1213 GAAGAG
MspA1I 357 CAGCTG C[AC]G^C[GT]G
2972 CCGCTG
NsiI 1823 ATGCAT ATGCA^T
1843 ATGCAT
PflMI 1458 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2995 CCAGCTCGTGG
PshBI 1858 ATTAAT AT^TAAT
2542 ATTAAT
SspBI 286 TGTACA T^GTACA
2198 TGTACA
Van91I 1458 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2995 CCAGCTCGTGG
XbaI 3565 TCTAGA T^CTAGA
3814 TCTAGA
XmnI 972 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2490 GAAAGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AcsI AfaI AflII AflIII AluI
AlwNI ApoI BanI BbsI BbvI
BfaI BfrI BmyI BpuAI BsaJI
BseRI BsiYI BslI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DraI EcoRII EcoT14I
Fnu4HI FokI HincII HindII HinfI
HphI ItaI MaeI MaeII MaeIII
MboII MnlI MseI MslI MvaI
MwoI NlaIII NlaIV NspI PleI
RsaI Sau96I ScrFI SfaNI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I