(Circular) (Six-base) MAPSORT of: pFN21AA0335.seq Check: 2168 from: 1 to: 9154 pFN21AA0335 9154 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6964 278 Size: 53 83 186 936 5428 2468 Fragments arranged by size: 5428 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6497 1937 Size: 4560 4594 Acc65I G'GTAC_C Cuts at: 3779 6475 3779 Size: 2696 6458 AclI AA'CG_TT Cuts at: 1563 7281 7654 1563 Size: 5718 373 3063 Fragments arranged by size: 5718 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 2735 3099 3237 4349 4368 5433 5940 Size: 708 395 364 138 1112 19 1065 507 Cuts at: 5940 7217 8601 8869 1632 Size: 1277 1384 268 1917 Fragments arranged by size: 1917 1384 1277 1112 1065 708 507 395 364 268 138 19 AflII C'TTAA_G Cuts at: 820 1017 2936 3047 4667 5357 6128 820 Size: 197 1919 111 1620 690 771 3846 Fragments arranged by size: 3846 1919 1620 771 690 197 111 AflIII A'CryG_T Cuts at: 1203 2842 6121 6444 8053 9030 1203 Size: 1639 3279 323 1609 977 1327 Fragments arranged by size: 3279 1639 1609 1327 977 323 AgeI A'CCGG_T Cuts at: 7985 7985 Size: 9154 AhdI GACnn_n'nnGTC Cuts at: 7883 7883 Size: 9154 AleI CACnn'nnGTG Cuts at: 1166 3041 1166 Size: 1875 7279 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9154 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3565 3597 3565 Size: 32 9122 AlwNI CAG_nnn'CTG Cuts at: 3431 5682 5832 6897 8469 3431 Size: 2251 150 1065 1572 4116 Fragments arranged by size: 4116 2251 1572 1065 150 ApaLI G'TGCA_C Cuts at: 7211 8367 7211 Size: 1156 7998 ApoI r'AATT_y Cuts at: 1493 2112 2697 3697 3710 4222 4334 4570 4577 Size: 619 585 1000 13 512 112 236 7 Cuts at: 4577 4644 4735 4771 5442 5763 6110 6347 6463 Size: 67 91 36 671 321 347 237 116 Cuts at: 6463 6689 6841 8950 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1000 671 619 585 512 347 321 237 236 226 152 116 112 91 67 36 13 7 AseI AT'TA_AT Cuts at: 160 1032 3864 4548 7708 160 Size: 872 2832 684 3160 1606 Fragments arranged by size: 3160 2832 1606 872 684 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9154 AvaI C'yCGr_G Cuts at: 1870 1943 3767 6432 6479 1870 Size: 73 1824 2665 47 4545 Fragments arranged by size: 4545 2665 1824 73 47 AvrII C'CTAG_G Cuts at: 5954 6026 5954 Size: 72 9082 BamHI G'GATC_C Cuts at: 6484 6851 6484 Size: 367 8787 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2098 2430 2813 3385 3779 Size: 325 217 627 311 332 383 572 394 Cuts at: 3779 4994 6475 7930 618 Size: 1215 1481 1455 1842 Fragments arranged by size: 1842 1481 1455 1215 627 572 394 383 332 325 311 217 BanII G_rGCy'C Cuts at: 1391 4076 6473 1391 Size: 2685 2397 4072 Fragments arranged by size: 4072 2685 2397 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2984 3087 3257 4263 6323 928 Size: 971 1085 103 170 1006 2060 3759 Fragments arranged by size: 3759 2060 1085 1006 971 170 103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 7345 7379 1903 Size: 34 50 34 5324 34 3678 Fragments arranged by size: 5324 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4910 6382 7046 8256 4910 Size: 1472 664 1210 5808 Fragments arranged by size: 5808 1472 1210 664 BclI T'GATC_A Cuts at: 1548 1548 Size: 9154 BfrBI ATG'CAT Cuts at: 3830 3850 3830 Size: 20 9134 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7765 136 Size: 107 122 71 631 605 6093 1525 Fragments arranged by size: 6093 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 9149 9149 Size: 9154 BlpI GC'TnA_GC Cuts at: 6571 6571 Size: 9154 Bme1580I G_kGCm'C Cuts at: 1790 4999 7215 8371 1790 Size: 3209 2216 1156 2573 Fragments arranged by size: 3209 2573 2216 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3207 4517 6885 Size: 416 53 83 399 24 1790 1310 2368 Cuts at: 6885 7843 442 Size: 958 1753 Fragments arranged by size: 2368 1790 1753 1310 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4298 7814 1239 Size: 3059 3516 2579 Fragments arranged by size: 3516 3059 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3024 7279 8144 8442 3024 Size: 4255 865 298 3736 Fragments arranged by size: 4255 3736 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7817 882 Size: 617 6318 2219 Fragments arranged by size: 6318 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6945 493 Size: 711 5741 2702 Fragments arranged by size: 5741 2702 711 BsaBI GATnn'nnATC Cuts at: 9148 9148 Size: 9154 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6961 7343 7978 275 Size: 53 83 186 936 5428 382 635 1451 Fragments arranged by size: 5428 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7587 7985 8259 8406 8817 7587 Size: 398 274 147 411 7924 Fragments arranged by size: 7924 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8929 Fragments arranged by size: 8929 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 3177 5562 1186 Size: 1114 877 2385 4778 Fragments arranged by size: 4778 2385 1114 877 BseYI C'CCAG_C Cuts at: 4999 8357 8853 4999 Size: 3358 496 5300 Fragments arranged by size: 5300 3358 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 8581 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6604 7365 8393 1540 Size: 275 186 4603 761 1028 2301 Fragments arranged by size: 4603 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4076 5225 7215 7300 8371 4076 Size: 1149 1990 85 1071 4859 Fragments arranged by size: 4859 1990 1149 1071 85 BsmI GAATG_Cn' Cuts at: 6665 6758 6665 Size: 93 9061 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4076 4999 5225 6473 7215 7300 8371 Size: 399 2286 923 226 1248 742 85 1071 Cuts at: 8371 1391 Size: 2174 Fragments arranged by size: 2286 2174 1248 1071 923 742 399 226 85 BspEI T'CCGG_A Cuts at: 8817 8817 Size: 9154 BspHI T'CATG_A Cuts at: 3246 7043 8773 3246 Size: 3797 1730 3627 Fragments arranged by size: 3797 3627 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6509 844 Size: 5665 3489 BsrBI CCG'CTC Cuts at: 1394 1823 7041 1394 Size: 429 5218 3507 Fragments arranged by size: 5218 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2294 5507 5885 7649 7823 65 Size: 1183 1046 3213 378 1764 174 1396 Fragments arranged by size: 3213 1764 1396 1183 1046 378 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7798 7985 1700 Size: 110 5988 187 2869 Fragments arranged by size: 5988 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2291 4203 96 Size: 2195 1912 5047 Fragments arranged by size: 5047 2195 1912 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9154 BssSI C'ACGA_G Cuts at: 4463 5004 7214 8226 4463 Size: 541 2210 1012 5391 Fragments arranged by size: 5391 2210 1012 541 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9154 BstBI TT'CG_AA Cuts at: 4493 7958 4493 Size: 3465 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9154 BstXI CCAn_nnnn'nTGG Cuts at: 2660 2660 Size: 9154 BstYI r'GATC_y Cuts at: 1972 6484 6851 7236 7253 8705 8791 8803 9149 Size: 4512 367 385 17 1452 86 12 346 Cuts at: 9149 1972 Size: 1977 Fragments arranged by size: 4512 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2434 513 Size: 552 1369 7233 Fragments arranged by size: 7233 1369 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8415 Fragments arranged by size: 8415 683 56 BtsI GCAGTG_nn' Cuts at: 3984 4543 6670 7464 7484 3984 Size: 559 2127 794 20 5654 Fragments arranged by size: 5654 2127 794 559 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3694 3729 529 Size: 35 3130 35 5954 Fragments arranged by size: 5954 3130 35 35 DraI TTT'AAA Cuts at: 2055 2370 2500 4113 4134 4770 5325 5460 6235 Size: 315 130 1613 21 636 555 135 775 Cuts at: 6235 6458 2055 Size: 223 4751 Fragments arranged by size: 4751 1613 775 636 555 315 223 135 130 21 DraIII CAC_nnn'GTG Cuts at: 2609 2609 Size: 9154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8161 809 Size: 507 6845 1802 Fragments arranged by size: 6845 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6601 7490 9119 Size: 54 1425 50 275 18 4771 889 1629 Cuts at: 9119 8 Size: 43 Fragments arranged by size: 4771 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6601 1537 Size: 275 4789 4090 Fragments arranged by size: 4789 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 3213 7084 1353 Size: 966 894 3871 3423 Fragments arranged by size: 3871 3423 966 894 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7741 8115 8261 387 Size: 1064 138 6152 374 146 1280 Fragments arranged by size: 6152 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4074 4074 Size: 9154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2340 2735 3099 3237 4298 4349 4368 Size: 393 708 395 364 138 1061 51 19 Cuts at: 4368 5433 5940 7217 7814 8601 8869 1239 Size: 1065 507 1277 597 787 268 1524 Fragments arranged by size: 1524 1277 1065 1061 787 708 597 507 395 393 364 268 138 51 19 EcoO109I rG'GnC_Cy Cuts at: 3266 5150 3266 Size: 1884 7270 EcoRI G'AATT_C Cuts at: 6463 6841 6463 Size: 378 8776 EcoRV GAT'ATC Cuts at: 3606 3606 Size: 9154 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2765 3672 4020 5207 2765 Size: 907 348 1187 6712 Fragments arranged by size: 6712 1187 907 348 FspI TGC'GCA Cuts at: 7660 7660 Size: 9154 HaeII r_GCGC'y Cuts at: 1678 8301 1678 Size: 6623 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5552 5584 7795 7827 7869 Size: 32 135 32 3980 32 2211 32 42 Cuts at: 7869 7901 1373 Size: 32 2626 Fragments arranged by size: 3980 2626 2211 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2206 3733 3808 6498 6744 669 Size: 988 281 268 1527 75 2690 246 3079 Fragments arranged by size: 3079 2690 1527 988 281 268 246 75 HindIII A'AGCT_T Cuts at: 748 748 Size: 9154 HpaI GTT'AAC Cuts at: 6744 6744 Size: 9154 KpnI G_GTAC'C Cuts at: 3783 6479 3783 Size: 2696 6458 MfeI C'AATT_G Cuts at: 4358 6753 4358 Size: 2395 6759 MluI A'CGCG_T Cuts at: 9030 9030 Size: 9154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3924 4051 8268 8452 1305 Size: 2619 127 4217 184 2007 Fragments arranged by size: 4217 2619 2007 184 127 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7332 Fragments arranged by size: 7332 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2871 3041 3111 3547 7112 7471 7630 Size: 648 1705 170 70 436 3565 359 159 Cuts at: 7630 518 Size: 2042 Fragments arranged by size: 3565 2042 1705 648 436 359 170 159 70 MspA1I CmG'CkG Cuts at: 1606 2364 4979 6570 7247 8395 8640 1606 Size: 758 2615 1591 677 1148 245 2120 Fragments arranged by size: 2615 2120 1591 1148 758 677 245 NcoI C'CATG_G Cuts at: 513 1065 2434 513 Size: 552 1369 7233 Fragments arranged by size: 7233 1369 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9154 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9154 NotI GC'GGCC_GC Cuts at: 6601 6601 Size: 9154 NsiI A_TGCA'T Cuts at: 3832 3852 3832 Size: 20 9134 NspI r_CATG'y Cuts at: 1132 1229 2846 5206 5646 6125 6448 6512 8057 Size: 97 1617 2360 440 479 323 64 1545 Cuts at: 8057 1132 Size: 2229 Fragments arranged by size: 2360 2229 1617 1545 479 440 323 97 64 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9154 PciI A'CATG_T Cuts at: 2842 6121 6444 8053 2842 Size: 3279 323 1609 3943 Fragments arranged by size: 3943 3279 1609 323 PflMI CCAn_nnn'nTGG Cuts at: 1108 3469 5006 1108 Size: 2361 1537 5256 Fragments arranged by size: 5256 2361 1537 PfoI T'CCnGG_A Cuts at: 4864 4864 Size: 9154 PmeI GTTT'AAAC Cuts at: 6458 6458 Size: 9154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7223 7255 8736 8768 7223 Size: 32 1481 32 7609 Fragments arranged by size: 7609 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3266 3266 Size: 9154 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9154 PsiI TTA'TAA Cuts at: 2706 2849 6183 6724 2706 Size: 143 3334 541 5136 Fragments arranged by size: 5136 3334 541 143 PstI C_TGCA'G Cuts at: 830 2932 6506 830 Size: 2102 3574 3478 Fragments arranged by size: 3574 3478 2102 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9154 PvuII CAG'CTG Cuts at: 2364 2364 Size: 9154 SacI G_AGCT'C Cuts at: 4076 4076 Size: 9154 SalI G'TCGA_C Cuts at: 1936 6496 1936 Size: 4560 4594 SbfI CC_TGCA'GG Cuts at: 6506 6506 Size: 9154 ScaI AGT'ACT Cuts at: 5383 7402 9114 5383 Size: 2019 1712 5423 Fragments arranged by size: 5423 2019 1712 SfcI C'TryA_G Cuts at: 826 1045 2928 6502 7637 8318 8509 826 Size: 219 1883 3574 1135 681 191 1471 Fragments arranged by size: 3574 1883 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9154 SmaI CCC'GGG Cuts at: 1872 6481 1872 Size: 4609 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2936 3039 3047 4667 5357 6128 Size: 197 926 993 103 8 1620 690 771 Cuts at: 6128 7258 8159 8421 820 Size: 1130 901 262 1553 Fragments arranged by size: 1620 1553 1130 993 926 901 771 690 262 197 103 8 SnaBI TAC'GTA Cuts at: 493 6945 493 Size: 6452 2702 SpeI A'CTAG_T Cuts at: 152 4057 152 Size: 3905 5249 SphI G_CATG'C Cuts at: 1132 6512 1132 Size: 5380 3774 SspI AAT'ATT Cuts at: 5 52 2173 2801 5433 6334 7078 5 Size: 47 2121 628 2632 901 744 2081 Fragments arranged by size: 2632 2121 2081 901 744 628 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9154 StyI C'CwwG_G Cuts at: 513 1065 2434 3904 5954 6026 6593 513 Size: 552 1369 1470 2050 72 567 3074 Fragments arranged by size: 3074 2050 1470 1369 567 552 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2666 7181 7198 7351 7536 7981 325 Size: 1255 1086 4515 17 153 185 445 1498 Fragments arranged by size: 4515 1498 1255 1086 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2291 3259 4203 Size: 274 80 33 51 1443 314 968 944 Cuts at: 4203 5381 6124 7400 9112 96 Size: 1178 743 1276 1712 138 Fragments arranged by size: 1712 1443 1276 1178 968 944 743 314 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3963 3995 4884 4916 9114 9146 3963 Size: 32 889 32 4198 32 3971 Fragments arranged by size: 4198 3971 889 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4675 1316 Size: 3359 5795 XbaI T'CTAG_A Cuts at: 5570 5819 6490 5570 Size: 249 671 8234 Fragments arranged by size: 8234 671 249 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9154 XmaI C'CCGG_G Cuts at: 1870 6479 1870 Size: 4609 4545 XmnI GAAnn'nnTTC Cuts at: 2981 4499 7283 2981 Size: 1518 2784 4852 Fragments arranged by size: 4852 2784 1518 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6962 276 Size: 53 83 186 936 5428 2468 Fragments arranged by size: 5428 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI