Restriction map for
pFN21AA0335
(Linear) (Six-base) MAPSORT of: KIAA0335.seq Check: 6278 from: 1 to: 4449
KIAA0335.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
Acc65I G'GTAC_C
Cuts at: 0 1774 4449
Size: 1774 2675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 730 1094 1232 2344 2363 3428 3935
Size: 335 395 364 138 1112 19 1065 507
Cuts at: 3935 4449
Size: 514
Fragments arranged by size:
1112 1065 514 507 395 364 335 138
19
AflII C'TTAA_G
Cuts at: 0 931 1042 2662 3352 4123 4449
Size: 931 111 1620 690 771 326
Fragments arranged by size:
1620 931 771 690 326 111
AflIII A'CryG_T
Cuts at: 0 837 4116 4439 4449
Size: 837 3279 323 10
Fragments arranged by size:
3279 837 323 10
AleI CACnn'nnGTG
Cuts at: 0 1036 4449
Size: 1036 3413
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1560 1592 4449
Size: 1560 32 2857
Fragments arranged by size:
2857 1560 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1426 3677 3827 4449
Size: 1426 2251 150 622
Fragments arranged by size:
2251 1426 622 150
ApoI r'AATT_y
Cuts at: 0 107 692 1692 1705 2217 2329 2565 2572
Size: 107 585 1000 13 512 112 236 7
Cuts at: 2572 2639 2730 2766 3437 3758 4105 4342 4449
Size: 67 91 36 671 321 347 237 107
Fragments arranged by size:
1000 671 585 512 347 321 237 236
112 107 107 91 67 36 13 7
AseI AT'TA_AT
Cuts at: 0 1859 2543 4449
Size: 1859 684 1906
Fragments arranged by size:
1906 1859 684
AvaI C'yCGr_G
Cuts at: 0 1762 4427 4449
Size: 1762 2665 22
Fragments arranged by size:
2665 1762 22
AvrII C'CTAG_G
Cuts at: 0 3949 4021 4449
Size: 3949 72 428
Fragments arranged by size:
3949 428 72
BanI G'GyrC_C
Cuts at: 0 93 425 808 1380 1774 2989 4449
Size: 93 332 383 572 394 1215 1460
Fragments arranged by size:
1460 1215 572 394 383 332 93
BanII G_rGCy'C
Cuts at: 0 2071 4449
Size: 2071 2378
BbsI GAAGACnn'nnnn_
Cuts at: 0 979 1082 1252 2258 4318 4449
Size: 979 103 170 1006 2060 131
Fragments arranged by size:
2060 1006 979 170 131 103
BciVI GTATCCnnnnn_n'
Cuts at: 0 2905 4377 4449
Size: 2905 1472 72
Fragments arranged by size:
2905 1472 72
BfrBI ATG'CAT
Cuts at: 0 1825 1845 4449
Size: 1825 20 2604
Fragments arranged by size:
2604 1825 20
Bme1580I G_kGCm'C
Cuts at: 0 2994 4449
Size: 2994 1455
BmrI ACTGGGnnnn_n'
Cuts at: 0 1202 2512 4449
Size: 1202 1310 1937
Fragments arranged by size:
1937 1310 1202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2293 4449
Size: 2293 2156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 4449
Size: 1019 3430
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 1172 3557 4449
Size: 295 877 2385 892
Fragments arranged by size:
2385 892 877 295
BseYI C'CCAG_C
Cuts at: 0 2994 4449
Size: 2994 1455
BsiHKAI G_wGCw'C
Cuts at: 0 2071 3220 4449
Size: 2071 1149 1229
Fragments arranged by size:
2071 1229 1149
Bsp1286I G_dGCh'C
Cuts at: 0 2071 2994 3220 4449
Size: 2071 923 226 1229
Fragments arranged by size:
2071 1229 923 226
BspHI T'CATG_A
Cuts at: 0 1241 4449
Size: 1241 3208
BsrDI GCAATG_nn'
Cuts at: 0 289 3502 3880 4449
Size: 289 3213 378 569
Fragments arranged by size:
3213 569 378 289
BsrGI T'GTAC_A
Cuts at: 0 286 2198 4449
Size: 286 1912 2251
Fragments arranged by size:
2251 1912 286
BssSI C'ACGA_G
Cuts at: 0 2458 2999 4449
Size: 2458 541 1450
Fragments arranged by size:
2458 1450 541
BstBI TT'CG_AA
Cuts at: 0 2488 4449
Size: 2488 1961
BstXI CCAn_nnnn'nTGG
Cuts at: 0 655 4449
Size: 655 3794
BtgI C'CryG_G
Cuts at: 0 429 4449
Size: 429 4020
BtsI GCAGTG_nn'
Cuts at: 0 1979 2538 4449
Size: 1979 559 1911
Fragments arranged by size:
1979 1911 559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1689 1724 4449
Size: 1689 35 2725
Fragments arranged by size:
2725 1689 35
DraI TTT'AAA
Cuts at: 0 50 365 495 2108 2129 2765 3320 3455
Size: 50 315 130 1613 21 636 555 135
Cuts at: 3455 4230 4449
Size: 775 219
Fragments arranged by size:
1613 775 636 555 315 219 135 130
50 21
DraIII CAC_nnn'GTG
Cuts at: 0 604 4449
Size: 604 3845
EarI CTCTTCn'nnn_
Cuts at: 0 314 1208 4449
Size: 314 894 3241
Fragments arranged by size:
3241 894 314
EcoICRI GAG'CTC
Cuts at: 0 2069 4449
Size: 2069 2380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 730 1094 1232 2293 2344 2363 3428
Size: 335 395 364 138 1061 51 19 1065
Cuts at: 3428 3935 4449
Size: 507 514
Fragments arranged by size:
1065 1061 514 507 395 364 335 138
51 19
EcoO109I rG'GnC_Cy
Cuts at: 0 1261 3145 4449
Size: 1261 1884 1304
Fragments arranged by size:
1884 1304 1261
EcoRV GAT'ATC
Cuts at: 0 1601 4449
Size: 1601 2848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 760 1667 2015 3202 4449
Size: 760 907 348 1187 1247
Fragments arranged by size:
1247 1187 907 760 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3547 3579 4449
Size: 3547 32 870
Fragments arranged by size:
3547 870 32
HincII GTy'rAC
Cuts at: 0 201 1728 1803 4449
Size: 201 1527 75 2646
Fragments arranged by size:
2646 1527 201 75
KpnI G_GTAC'C
Cuts at: 0 1778 4449
Size: 1778 2671
MfeI C'AATT_G
Cuts at: 0 2353 4449
Size: 2353 2096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1919 2046 4449
Size: 1919 127 2403
Fragments arranged by size:
2403 1919 127
MslI CAynn'nnrTG
Cuts at: 0 866 1036 1106 1542 4449
Size: 866 170 70 436 2907
Fragments arranged by size:
2907 866 436 170 70
MspA1I CmG'CkG
Cuts at: 0 359 2974 4449
Size: 359 2615 1475
Fragments arranged by size:
2615 1475 359
NcoI C'CATG_G
Cuts at: 0 429 4449
Size: 429 4020
NsiI A_TGCA'T
Cuts at: 0 1827 1847 4449
Size: 1827 20 2602
Fragments arranged by size:
2602 1827 20
NspI r_CATG'y
Cuts at: 0 841 3201 3641 4120 4443 4449
Size: 841 2360 440 479 323 6
Fragments arranged by size:
2360 841 479 440 323 6
PciI A'CATG_T
Cuts at: 0 837 4116 4439 4449
Size: 837 3279 323 10
Fragments arranged by size:
3279 837 323 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 1464 3001 4449
Size: 1464 1537 1448
Fragments arranged by size:
1537 1464 1448
PfoI T'CCnGG_A
Cuts at: 0 2859 4449
Size: 2859 1590
PpuMI rG'GwC_Cy
Cuts at: 0 1261 4449
Size: 1261 3188
PsiI TTA'TAA
Cuts at: 0 701 844 4178 4449
Size: 701 143 3334 271
Fragments arranged by size:
3334 701 271 143
PstI C_TGCA'G
Cuts at: 0 927 4449
Size: 927 3522
PvuII CAG'CTG
Cuts at: 0 359 4449
Size: 359 4090
SacI G_AGCT'C
Cuts at: 0 2071 4449
Size: 2071 2378
ScaI AGT'ACT
Cuts at: 0 3378 4449
Size: 3378 1071
SfcI C'TryA_G
Cuts at: 0 923 4449
Size: 923 3526
SmlI C'TyrA_G
Cuts at: 0 931 1034 1042 2662 3352 4123 4449
Size: 931 103 8 1620 690 771 326
Fragments arranged by size:
1620 931 771 690 326 103 8
SpeI A'CTAG_T
Cuts at: 0 2052 4449
Size: 2052 2397
SspI AAT'ATT
Cuts at: 0 168 796 3428 4329 4449
Size: 168 628 2632 901 120
Fragments arranged by size:
2632 901 628 168 120
StyI C'CwwG_G
Cuts at: 0 429 1899 3949 4021 4449
Size: 429 1470 2050 72 428
Fragments arranged by size:
2050 1470 429 428 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 661 4449
Size: 661 3788
TatI w'GTAC_w
Cuts at: 0 286 1254 2198 3376 4119 4449
Size: 286 968 944 1178 743 330
Fragments arranged by size:
1178 968 944 743 330 286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1958 1990 2879 2911 4449
Size: 1958 32 889 32 1538
Fragments arranged by size:
1958 1538 889 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2670 4449
Size: 2670 1779
XbaI T'CTAG_A
Cuts at: 0 3565 3814 4449
Size: 3565 249 635
Fragments arranged by size:
3565 635 249
XmnI GAAnn'nnTTC
Cuts at: 0 976 2494 4449
Size: 976 1518 1955
Fragments arranged by size:
1955 1518 976
Enzymes that do cut:
Acc65I AcuI AflII AflIII AleI AloI AlwNI ApoI
AseI AvaI AvrII BanI BanII BbsI BciVI BfrBI
Bme1580I BmrI BpmI BpuEI BseRI BseYI BsiHKAI Bsp1286I
BspHI BsrDI BsrGI BssSI BstBI BstXI BtgI BtsI
CspCI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRV
FalI Hin4I HincII KpnI MfeI MmeI MslI MspA1I
NcoI NsiI NspI PciI PflMI PfoI PpuMI PsiI
PstI PvuII SacI ScaI SfcI SmlI SpeI SspI
StyI TaqII TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AlfI
ApaI ApaLI AscI AsiSI BaeI BamHI BbeI BbvCI
BcgI BclI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI
BssHII BstAPI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
DrdI EaeI EagI EciI EcoNI EcoRI FseI FspI
FspAI HaeII HindIII HpaI KasI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI
SwaI XcmI XhoI XmaI ZraI