Restriction map for
pFN21AA0288
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AclI AflII AgeI AocI
Aor51HI AscI AseI AsnI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsiWI BsmI BspDI
BspHI BsrDI BssHII Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KasI MfeI MluI
MseI MunI NarI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsrII SfiI SfoI
SfuI SnaBI SpeI SphI SspI
SwaI Tru9I XbaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 2861 GGTACC G^GTACC
AccI 663 GTCGAC GT^[AC][GT]AC
AccIII 892 TCCGGA T^CCGGA
AcsI 529 GAATTT [GA]^AATT[CT]
AflIII 2576 ACATGT A^C[GA][CT]GT
AhdI 1339 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2647 GGGCCC GGGCC^C
ApoI 529 GAATTT [GA]^AATT[CT]
Asp700I 2604 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2861 GGTACC G^GTACC
AspEI 1339 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1392 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 2344 GTCTTC GAAGAC(2/6)
BbvCI 1812 GCTGAGG CCTCAGC(-5/-2)
BfaI 2215 CTAG C^TAG
BpuAI 2344 GTCTTC GAAGAC(2/6)
BseAI 892 TCCGGA T^CCGGA
Bsp1407I 2573 TGTACA T^GTACA
BspEI 892 TCCGGA T^CCGGA
BspLU11I 2576 ACATGT A^CATGT
BsrGI 2573 TGTACA T^GTACA
BstEII 3165 GGTCACC G^GT[ACGT]ACC
BstPI 3165 GGTCACC G^GT[ACGT]ACC
DrdI 2560 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1339 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3165 GGTCACC G^GT[ACGT]ACC
HindIII 2467 AAGCTT A^AGCTT
KpnI 2861 GGTACC GGTAC^C
KspI 2103 CCGCGG CCGC^GG
MaeI 2215 CTAG C^TAG
MroI 892 TCCGGA T^CCGGA
NdeI 2280 CATATG CA^TATG
PaeR7I 2914 CTCGAG C^TCGAG
PflFI 1392 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 42 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2536 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 2750 CGATCG CGAT^CG
SacII 2103 CCGCGG CCGC^GG
SalI 663 GTCGAC G^TCGAC
SapI 1764 GCTCTTC GCTCTTC(1/4)
SbfI 2064 CCTGCAGG CCTGCA^GG
SexAI 1436 ACCTGGT A^CC[AT]GGT
SgrAI 2682 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1883 CCCGGG CCC^GGG
Sse8387I 2064 CCTGCAGG CCTGCA^GG
SspBI 2573 TGTACA T^GTACA
Tth111I 1392 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 42 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1055 CCAACATCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2914 CTCGAG C^TCGAG
XmaCI 1883 CCCGGG C^CCGGG
XmaI 1883 CCCGGG C^CCGGG
XmnI 2604 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BamHI 1802 GGATCC G^GATCC
2042 GGATCC
BsaBI 261 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2499 GATCCTCATC
BsaI 38 GAGACC GGTCTC(1/5)
3213 GAGACC
BsaWI 563 ACCGGA [AT]^CCGG[AT]
892 TCCGGA
BstXI 15 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
86 CCAGCACGGTGG
DraII 2536 GGGACCC [GA]G^G[ACGT]CC[CT]
2646 AGGGCCC
EagI 1016 CGGCCG C^GGCCG
2895 CGGCCG
EclXI 1016 CGGCCG C^GGCCG
2895 CGGCCG
Eco52I 1016 CGGCCG C^GGCCG
2895 CGGCCG
EcoO109I 2536 GGGACCC [GA]G^G[ACGT]CC[CT]
2646 AGGGCCC
HincII 663 GTCGAC GT[CT]^[GA]AC
2668 GTCAAC
HindII 663 GTCGAC GT[CT]^[GA]AC
2668 GTCAAC
MaeII 2519 ACGT A^CGT
2666 ACGT
MamI 261 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2499 GATCCTCATC
PleI 865 GACTC GAGTC(4/5)
1124 GACTC
ScaI 2717 AGTACT AGT^ACT
3077 AGTACT
Tsp509I 530 AATT ^AATT
752 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII