(Circular) (Six-base) MAPSORT of: pFN21AA0288.seq Check: 9633 from: 1 to: 7957 pFN21AA0288 7957 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3664 4016 3664 Size: 352 7605 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5767 278 Size: 53 83 186 936 4231 2468 Fragments arranged by size: 4231 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2669 5300 1937 Size: 732 2631 4594 Fragments arranged by size: 4594 2631 732 Acc65I G'GTAC_C Cuts at: 4866 5278 4866 Size: 412 7545 AclI AA'CG_TT Cuts at: 1563 6084 6457 1563 Size: 4521 373 3063 Fragments arranged by size: 4521 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3757 4013 4394 4720 6020 7404 7672 1632 Size: 2125 256 381 326 1300 1384 268 1917 Fragments arranged by size: 2125 1917 1384 1300 381 326 268 256 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7760 AflIII A'CryG_T Cuts at: 1203 4581 6856 7833 1203 Size: 3378 2275 977 1327 Fragments arranged by size: 3378 2275 1327 977 AgeI A'CCGG_T Cuts at: 6788 6788 Size: 7957 AhdI GACnn_n'nnGTC Cuts at: 3349 6686 3349 Size: 3337 4620 AleI CACnn'nnGTG Cuts at: 1166 2513 1166 Size: 1347 6610 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7957 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2156 2188 2156 Size: 32 7925 AlwNI CAG_nnn'CTG Cuts at: 2215 2383 2431 2458 2741 3433 3442 3472 3508 Size: 168 48 27 283 692 9 30 36 Cuts at: 3508 3607 3766 4201 5700 7272 2215 Size: 99 159 435 1499 1572 2900 Fragments arranged by size: 2900 1572 1499 692 435 283 168 159 99 48 36 30 27 9 ApaI G_GGCC'C Cuts at: 4656 4656 Size: 7957 ApaLI G'TGCA_C Cuts at: 4003 4145 4298 4526 6014 7170 4003 Size: 142 153 228 1488 1156 4790 Fragments arranged by size: 4790 1488 1156 228 153 142 ApoI r'AATT_y Cuts at: 1493 2534 5266 5492 5644 7753 1493 Size: 1041 2732 226 152 2109 1697 Fragments arranged by size: 2732 2109 1697 1041 226 152 AseI AT'TA_AT Cuts at: 160 1032 6511 160 Size: 872 5479 1606 Fragments arranged by size: 5479 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7957 AvaI C'yCGr_G Cuts at: 1870 1943 3888 4031 4919 5282 1870 Size: 73 1945 143 888 363 4545 Fragments arranged by size: 4545 1945 888 363 143 73 BamHI G'GATC_C Cuts at: 3807 4047 5287 5654 3807 Size: 240 1240 367 6110 Fragments arranged by size: 6110 1240 367 240 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2412 3173 4866 5278 6733 Size: 325 217 627 625 761 1693 412 1455 Cuts at: 6733 618 Size: 1842 Fragments arranged by size: 1842 1693 1455 761 627 625 412 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2229 3591 3621 3690 3720 3942 4633 Size: 621 217 1362 30 69 30 222 691 Cuts at: 4633 4656 5235 5276 1391 Size: 23 579 41 4072 Fragments arranged by size: 4072 1362 691 621 579 222 217 69 41 30 30 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4342 928 Size: 971 2443 4543 Fragments arranged by size: 4543 2443 971 BbvCI CC'TCA_GC Cuts at: 3818 3818 Size: 7957 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6148 6182 1903 Size: 34 4211 34 3678 Fragments arranged by size: 4211 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5849 7059 5849 Size: 1210 6747 BclI T'GATC_A Cuts at: 1548 1548 Size: 7957 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2138 3550 4089 Size: 107 122 71 631 605 466 1412 539 Cuts at: 4089 4322 4896 6568 136 Size: 233 574 1672 1525 Fragments arranged by size: 1672 1525 1412 631 605 574 539 466 233 122 107 71 BglII A'GATC_T Cuts at: 7952 7952 Size: 7957 BlpI GC'TnA_GC Cuts at: 5374 5374 Size: 7957 Bme1580I G_kGCm'C Cuts at: 1790 2148 3104 4007 4149 4302 4530 4649 4656 Size: 358 956 903 142 153 228 119 7 Cuts at: 4656 6018 7174 1790 Size: 1362 1156 2573 Fragments arranged by size: 2573 1362 1156 956 903 358 228 153 142 119 7 BmgBI CAC'GTC Cuts at: 1318 4525 1318 Size: 3207 4750 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3394 3398 3747 Size: 416 53 83 399 24 1977 4 349 Cuts at: 3747 4528 5688 6646 442 Size: 781 1160 958 1753 Fragments arranged by size: 1977 1753 1160 958 781 416 399 349 83 53 24 4 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7957 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2320 4318 2320 Size: 1998 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2426 2453 3131 3284 3494 3815 4148 6617 Size: 1187 27 678 153 210 321 333 2469 Cuts at: 6617 1239 Size: 2579 Fragments arranged by size: 2579 2469 1187 678 333 321 210 153 27 Bpu10I CC'TnA_GC Cuts at: 3194 3818 3194 Size: 624 7333 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2222 2453 6082 6947 7245 2222 Size: 231 3629 865 298 2934 Fragments arranged by size: 3629 2934 865 298 231 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2037 5212 6620 882 Size: 617 538 3175 1408 2219 Fragments arranged by size: 3175 2219 1408 617 538 BsaAI yAC'GTr Cuts at: 493 1204 5748 493 Size: 711 4544 2702 Fragments arranged by size: 4544 2702 711 BsaBI GATnn'nnATC Cuts at: 2270 4508 7951 2270 Size: 2238 3443 2276 Fragments arranged by size: 3443 2276 2238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2706 3120 3833 4953 Size: 53 83 186 936 1173 414 713 1120 Cuts at: 4953 5764 6146 6781 275 Size: 811 382 635 1451 Fragments arranged by size: 1451 1173 1120 936 811 713 635 414 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2568 2897 6390 6788 7062 7209 7620 2568 Size: 329 3493 398 274 147 411 2905 Fragments arranged by size: 3493 2905 411 398 329 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2906 2936 3300 3330 4436 Size: 30 165 30 1503 30 364 30 1106 Cuts at: 4436 4466 4832 4862 1178 Size: 30 366 30 4273 Fragments arranged by size: 4273 1503 1106 366 364 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3587 3596 3599 4145 4433 5243 5258 1186 Size: 2401 9 3 546 288 810 15 3885 Fragments arranged by size: 3885 2401 810 546 288 15 9 3 BseYI C'CCAG_C Cuts at: 2090 2903 2966 3070 4545 4568 4981 7160 7656 Size: 813 63 104 1475 23 413 2179 496 Cuts at: 7656 2090 Size: 2391 Fragments arranged by size: 2391 2179 1475 813 496 413 104 63 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2899 3360 3692 3953 1163 Size: 573 1163 461 332 261 5167 Fragments arranged by size: 5167 1163 573 461 332 261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3024 4758 4903 5407 6168 7196 Size: 275 186 1023 1734 145 504 761 1028 Cuts at: 7196 1540 Size: 2301 Fragments arranged by size: 2301 1734 1028 1023 761 504 275 186 145 BsiHKAI G_wGCw'C Cuts at: 2012 2229 2337 3200 3310 3591 3600 4007 4038 Size: 217 108 863 110 281 9 407 31 Cuts at: 4038 4149 4302 4427 4530 6018 6103 7174 2012 Size: 111 153 125 103 1488 85 1071 2795 Fragments arranged by size: 2795 1488 1071 863 407 281 217 153 125 111 110 108 103 85 31 9 BsmI GAATG_Cn' Cuts at: 5468 5561 5468 Size: 93 7864 BsmBI CGTCTCn'nnnn_ Cuts at: 2975 3569 4070 4707 5159 2975 Size: 594 501 637 452 5773 Fragments arranged by size: 5773 637 594 501 452 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2148 2229 2337 3104 3200 3310 Size: 399 222 136 81 108 767 96 110 Cuts at: 3310 3591 3600 3621 3690 3720 3942 4007 4038 Size: 281 9 21 69 30 222 65 31 Cuts at: 4038 4149 4302 4427 4530 4633 4649 4656 5235 Size: 111 153 125 103 103 16 7 579 Cuts at: 5235 5276 6018 6103 7174 1391 Size: 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 767 742 579 399 281 222 222 153 136 125 111 110 108 103 103 96 85 81 69 65 41 31 30 21 16 9 7 BspEI T'CCGG_A Cuts at: 2897 7620 2897 Size: 4723 3234 BspHI T'CATG_A Cuts at: 5846 7576 5846 Size: 1730 6227 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 3664 4016 5312 844 Size: 1319 1501 352 1296 3489 Fragments arranged by size: 3489 1501 1319 1296 352 BsrBI CCG'CTC Cuts at: 1394 1823 2661 3026 3214 5844 1394 Size: 429 838 365 188 2630 3507 Fragments arranged by size: 3507 2630 838 429 365 188 BsrDI GCAATG_nn' Cuts at: 65 1248 6452 6626 65 Size: 1183 5204 174 1396 Fragments arranged by size: 5204 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2194 3086 3274 3630 3665 3848 4191 Size: 110 384 892 188 356 35 183 343 Cuts at: 4191 4688 6601 6788 1700 Size: 497 1913 187 2869 Fragments arranged by size: 2869 1913 892 497 384 356 343 188 187 183 110 35 BsrGI T'GTAC_A Cuts at: 96 4578 96 Size: 4482 3475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7957 BssSI C'ACGA_G Cuts at: 2294 2315 2330 3009 3971 6017 7029 2294 Size: 21 15 679 962 2046 1012 3222 Fragments arranged by size: 3222 2046 1012 962 679 21 15 BstAPI GCAn_nnn'nTGC Cuts at: 794 2215 2383 2458 3508 3766 794 Size: 1421 168 75 1050 258 4985 Fragments arranged by size: 4985 1421 1050 258 168 75 BstBI TT'CG_AA Cuts at: 6761 6761 Size: 7957 BstEII G'GTnAC_C Cuts at: 1184 5170 1184 Size: 3986 3971 BstXI CCAn_nnnn'nTGG Cuts at: 2027 2098 2027 Size: 71 7886 BstYI r'GATC_y Cuts at: 1972 2253 2265 3807 4047 4503 5070 5287 5654 Size: 281 12 1542 240 456 567 217 367 Cuts at: 5654 6039 6056 7508 7594 7606 7952 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1542 1452 567 456 385 367 346 281 240 217 86 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2109 2148 2499 3722 3837 4108 4431 Size: 552 1044 39 351 1223 115 271 323 Cuts at: 4431 4455 4461 4531 4707 4809 5052 5178 5193 Size: 24 6 70 176 102 243 126 15 Cuts at: 5193 5235 513 Size: 42 3235 Fragments arranged by size: 3235 1223 1044 552 351 323 271 243 176 126 115 102 70 42 39 24 15 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2257 3030 3714 487 Size: 683 56 1031 773 684 4730 Fragments arranged by size: 4730 1031 773 684 683 56 BtsI GCAGTG_nn' Cuts at: 2148 2177 2581 2835 3308 5473 6267 6287 2148 Size: 29 404 254 473 2165 794 20 3818 Fragments arranged by size: 3818 2165 794 473 404 254 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7957 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4813 4848 529 Size: 35 4249 35 3638 Fragments arranged by size: 4249 3638 35 35 DraI TTT'AAA Cuts at: 5261 5261 Size: 7957 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4571 6964 809 Size: 507 3255 2393 1802 Fragments arranged by size: 3255 2393 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 2106 2361 Size: 54 1425 50 275 18 208 68 255 Cuts at: 2361 2502 2784 3021 3871 4359 4458 4900 5404 Size: 141 282 237 850 488 99 442 504 Cuts at: 5404 6293 7922 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 850 504 488 442 282 275 255 237 208 141 99 68 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3021 4900 5404 1537 Size: 275 1209 1879 504 4090 Fragments arranged by size: 4090 1879 1209 504 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3246 3702 3776 5235 5887 1353 Size: 1893 456 74 1459 652 3423 Fragments arranged by size: 3423 1893 1459 652 456 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2993 3409 4385 4840 6544 6918 Size: 1064 138 1404 416 976 455 1704 374 Cuts at: 6918 7064 387 Size: 146 1280 Fragments arranged by size: 1704 1404 1280 1064 976 455 416 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2227 3589 2010 Size: 217 1362 6378 Fragments arranged by size: 6378 1362 217 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2426 2453 3131 3284 3494 3757 3815 Size: 393 794 27 678 153 210 263 58 Cuts at: 3815 4013 4148 4394 4720 6020 6617 7404 7672 Size: 198 135 246 326 1300 597 787 268 Cuts at: 7672 1239 Size: 1524 Fragments arranged by size: 1524 1300 794 787 678 597 393 326 268 263 246 210 198 153 135 58 27 EcoO109I rG'GnC_Cy Cuts at: 4542 4652 4542 Size: 110 7847 EcoRI G'AATT_C Cuts at: 5266 5644 5266 Size: 378 7579 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4399 4399 Size: 7957 FspI TGC'GCA Cuts at: 6463 6463 Size: 7957 HaeII r_GCGC'y Cuts at: 1678 2117 2237 2561 2889 3748 5092 7104 1678 Size: 439 120 324 328 859 1344 2012 2531 Fragments arranged by size: 2531 2012 1344 859 439 328 324 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3335 3367 5122 5154 6598 Size: 32 135 32 1763 32 1755 32 1444 Cuts at: 6598 6630 6672 6704 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1763 1755 1444 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2670 4675 5301 5547 669 Size: 988 281 732 2005 626 246 3079 Fragments arranged by size: 3079 2005 988 732 626 281 246 HindIII A'AGCT_T Cuts at: 748 4472 748 Size: 3724 4233 HpaI GTT'AAC Cuts at: 5547 5547 Size: 7957 KpnI G_GTAC'C Cuts at: 4870 5282 4870 Size: 412 7545 MfeI C'AATT_G Cuts at: 5556 5556 Size: 7957 MluI A'CGCG_T Cuts at: 7833 7833 Size: 7957 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2838 7071 7255 1305 Size: 1533 4233 184 2007 Fragments arranged by size: 4233 2007 1533 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 2363 2504 4361 10 Size: 54 1425 343 276 255 141 1857 3606 Fragments arranged by size: 3606 1857 1425 343 276 255 141 54 MslI CAynn'nnrTG Cuts at: 518 1166 2025 2513 3857 3986 4430 5177 5915 Size: 648 859 488 1344 129 444 747 738 Cuts at: 5915 6274 6433 518 Size: 359 159 2042 Fragments arranged by size: 2042 1344 859 747 738 648 488 444 359 159 129 MspA1I CmG'CkG Cuts at: 1606 2215 2398 3412 3805 3817 4110 4626 4882 Size: 609 183 1014 393 12 293 516 256 Cuts at: 4882 5117 5373 6050 7198 7443 1606 Size: 235 256 677 1148 245 2120 Fragments arranged by size: 2120 1148 1014 677 609 516 393 293 256 256 245 235 183 12 NaeI GCC'GGC Cuts at: 2196 3276 3667 3850 2196 Size: 1080 391 183 6303 Fragments arranged by size: 6303 1080 391 183 NcoI C'CATG_G Cuts at: 513 1065 2148 3837 4431 4531 4707 5052 5178 Size: 552 1083 1689 594 100 176 345 126 Cuts at: 5178 5235 513 Size: 57 3235 Fragments arranged by size: 3235 1689 1083 594 552 345 176 126 100 57 NdeI CA'TA_TG Cuts at: 387 4286 387 Size: 3899 4058 NgoMIV G'CCGG_C Cuts at: 2194 3274 3665 3848 2194 Size: 1080 391 183 6303 Fragments arranged by size: 6303 1080 391 183 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7957 NotI GC'GGCC_GC Cuts at: 5404 5404 Size: 7957 NspI r_CATG'y Cuts at: 1132 1229 2089 4585 4968 5315 6860 1132 Size: 97 860 2496 383 347 1545 2229 Fragments arranged by size: 2496 2229 1545 860 383 347 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7957 PciI A'CATG_T Cuts at: 4581 6856 4581 Size: 2275 5682 PflMI CCAn_nnn'nTGG Cuts at: 1108 2053 1108 Size: 945 7012 PfoI T'CCnGG_A Cuts at: 2924 2924 Size: 7957 PmeI GTTT'AAAC Cuts at: 5261 5261 Size: 7957 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6026 6058 7539 7571 6026 Size: 32 1481 32 6412 Fragments arranged by size: 6412 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4542 4542 Size: 7957 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7957 PsiI TTA'TAA Cuts at: 5527 5527 Size: 7957 PspOMI G'GGCC_C Cuts at: 4652 4652 Size: 7957 PspXI vC'TCGA_Gb Cuts at: 4919 4919 Size: 7957 PstI C_TGCA'G Cuts at: 830 2220 2463 3249 3438 3513 4074 4482 5100 Size: 1390 243 786 189 75 561 408 618 Cuts at: 5100 5309 830 Size: 209 3478 Fragments arranged by size: 3478 1390 786 618 561 408 243 209 189 75 PvuI CG_AT'CG Cuts at: 2001 4758 2001 Size: 2757 5200 PvuII CAG'CTG Cuts at: 2215 3817 4882 2215 Size: 1602 1065 5290 Fragments arranged by size: 5290 1602 1065 SacI G_AGCT'C Cuts at: 2012 2229 3591 2012 Size: 217 1362 6378 Fragments arranged by size: 6378 1362 217 SacII CC_GC'GG Cuts at: 4111 4111 Size: 7957 SalI G'TCGA_C Cuts at: 1936 2668 5299 1936 Size: 732 2631 4594 Fragments arranged by size: 4594 2631 732 SanDI GG'GwC_CC Cuts at: 4542 4542 Size: 7957 SapI GCTCTTCn'nnn_ Cuts at: 3776 3776 Size: 7957 SbfI CC_TGCA'GG Cuts at: 4074 5309 4074 Size: 1235 6722 ScaI AGT'ACT Cuts at: 4724 5084 6205 7917 4724 Size: 360 1121 1712 4764 Fragments arranged by size: 4764 1712 1121 360 SexAI A'CCwGG_T Cuts at: 3441 3441 Size: 7957 SfcI C'TryA_G Cuts at: 826 1045 2216 2459 3245 3434 3509 4070 4136 Size: 219 1171 243 786 189 75 561 66 Cuts at: 4136 4478 4558 5096 5305 6440 7121 7312 826 Size: 342 80 538 209 1135 681 191 1471 Fragments arranged by size: 1471 1171 1135 786 681 561 538 342 243 219 209 191 189 80 75 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7957 SgrAI Cr'CCGG_yG Cuts at: 1700 4688 1700 Size: 2988 4969 SmaI CCC'GGG Cuts at: 1872 3890 5284 1872 Size: 2018 1394 4545 Fragments arranged by size: 4545 2018 1394 SmlI C'TyrA_G Cuts at: 820 1017 1943 2237 2468 4919 6061 6962 7224 Size: 197 926 294 231 2451 1142 901 262 Cuts at: 7224 820 Size: 1553 Fragments arranged by size: 2451 1553 1142 926 901 294 262 231 197 SnaBI TAC'GTA Cuts at: 493 5748 493 Size: 5255 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7957 SphI G_CATG'C Cuts at: 1132 5315 1132 Size: 4183 3774 SspI AAT'ATT Cuts at: 5 52 5881 5 Size: 47 5829 2081 Fragments arranged by size: 5829 2081 47 StuI AGG'CCT Cuts at: 1517 3300 3880 3969 1517 Size: 1783 580 89 5505 Fragments arranged by size: 5505 1783 580 89 StyI C'CwwG_G Cuts at: 513 1065 2148 3207 3330 3837 4431 4531 4707 Size: 552 1083 1059 123 507 594 100 176 Cuts at: 4707 5052 5178 5235 5396 513 Size: 345 126 57 161 3074 Fragments arranged by size: 3074 1083 1059 594 552 507 345 176 161 126 123 100 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2926 3191 5984 6001 6154 6339 6784 Size: 1255 1346 265 2793 17 153 185 445 Cuts at: 6784 325 Size: 1498 Fragments arranged by size: 2793 1498 1346 1255 445 265 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4578 4722 5082 Size: 274 80 33 51 1443 2601 144 360 Cuts at: 5082 6203 7915 96 Size: 1121 1712 138 Fragments arranged by size: 2601 1712 1443 1121 360 274 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2573 2605 3300 3311 3332 3343 7917 7949 2573 Size: 32 695 11 21 11 4574 32 2581 Fragments arranged by size: 4574 2581 695 32 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 3400 1316 Size: 2084 5873 XbaI T'CTAG_A Cuts at: 5293 5293 Size: 7957 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3067 3067 Size: 7957 XhoI C'TCGA_G Cuts at: 1943 4919 1943 Size: 2976 4981 XmaI C'CCGG_G Cuts at: 1870 3888 5282 1870 Size: 2018 1394 4545 Fragments arranged by size: 4545 2018 1394 XmnI GAAnn'nnTTC Cuts at: 4613 6086 4613 Size: 1473 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5765 276 Size: 53 83 186 936 4231 2468 Fragments arranged by size: 4231 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SfiI SfoI SrfI SwaI