Restriction map for
pFN21AA0288
(Linear) (Six-base) MAPSORT of: KIAA0288.seq Check: 7937 from: 1 to: 3252
KIAA0288.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1659 2011 3252
Size: 1659 352 1241
Fragments arranged by size:
1659 1241 352
AccI GT'mk_AC
Cuts at: 0 664 3252
Size: 664 2588
Acc65I G'GTAC_C
Cuts at: 0 2861 3252
Size: 2861 391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1752 2008 2389 2715 3252
Size: 1752 256 381 326 537
Fragments arranged by size:
1752 537 381 326 256
AflIII A'CryG_T
Cuts at: 0 2576 3252
Size: 2576 676
AhdI GACnn_n'nnGTC
Cuts at: 0 1344 3252
Size: 1344 1908
AleI CACnn'nnGTG
Cuts at: 0 508 3252
Size: 508 2744
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 151 183 3252
Size: 151 32 3069
Fragments arranged by size:
3069 151 32
AlwNI CAG_nnn'CTG
Cuts at: 0 210 378 426 453 736 1428 1437 1467
Size: 210 168 48 27 283 692 9 30
Cuts at: 1467 1503 1602 1761 2196 3252
Size: 36 99 159 435 1056
Fragments arranged by size:
1056 692 435 283 210 168 159 99
48 36 30 27 9
ApaI G_GGCC'C
Cuts at: 0 2651 3252
Size: 2651 601
ApaLI G'TGCA_C
Cuts at: 0 1998 2140 2293 2521 3252
Size: 1998 142 153 228 731
Fragments arranged by size:
1998 731 228 153 142
ApoI r'AATT_y
Cuts at: 0 529 3252
Size: 529 2723
AvaI C'yCGr_G
Cuts at: 0 1883 2026 2914 3252
Size: 1883 143 888 338
Fragments arranged by size:
1883 888 338 143
BamHI G'GATC_C
Cuts at: 0 1802 2042 3252
Size: 1802 240 1210
Fragments arranged by size:
1802 1210 240
BanI G'GyrC_C
Cuts at: 0 407 1168 2861 3252
Size: 407 761 1693 391
Fragments arranged by size:
1693 761 407 391
BanII G_rGCy'C
Cuts at: 0 7 224 1586 1616 1685 1715 1937 2628
Size: 7 217 1362 30 69 30 222 691
Cuts at: 2628 2651 3230 3252
Size: 23 579 22
Fragments arranged by size:
1362 691 579 222 217 69 30 30
23 22 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 2337 3252
Size: 2337 915
BbvCI CC'TCA_GC
Cuts at: 0 1813 3252
Size: 1813 1439
BglI GCCn_nnn'nGGC
Cuts at: 0 133 1545 2084 2317 2891 3252
Size: 133 1412 539 233 574 361
Fragments arranged by size:
1412 574 539 361 233 133
Bme1580I G_kGCm'C
Cuts at: 0 143 1099 2002 2144 2297 2525 2644 2651
Size: 143 956 903 142 153 228 119 7
Cuts at: 2651 3252
Size: 601
Fragments arranged by size:
956 903 601 228 153 143 142 119
7
BmgBI CAC'GTC
Cuts at: 0 2520 3252
Size: 2520 732
BmrI ACTGGGnnnn_n'
Cuts at: 0 1389 1393 1742 2523 3252
Size: 1389 4 349 781 729
Fragments arranged by size:
1389 781 729 349 4
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 315 2313 3252
Size: 315 1998 939
Fragments arranged by size:
1998 939 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 448 1126 1279 1489 1810 2143 3252
Size: 421 27 678 153 210 321 333 1109
Fragments arranged by size:
1109 678 421 333 321 210 153 27
Bpu10I CC'TnA_GC
Cuts at: 0 1189 1813 3252
Size: 1189 624 1439
Fragments arranged by size:
1439 1189 624
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 448 3252
Size: 217 231 2804
Fragments arranged by size:
2804 231 217
BsaI GGTCTCn'nnnn_
Cuts at: 0 32 3207 3252
Size: 32 3175 45
Fragments arranged by size:
3175 45 32
BsaBI GATnn'nnATC
Cuts at: 0 265 2503 3252
Size: 265 2238 749
Fragments arranged by size:
2238 749 265
BsaHI Gr'CG_yC
Cuts at: 0 701 1115 1828 2948 3252
Size: 701 414 713 1120 304
Fragments arranged by size:
1120 713 701 414 304
BsaWI w'CCGG_w
Cuts at: 0 563 892 3252
Size: 563 329 2360
Fragments arranged by size:
2360 563 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 901 931 1295 1325 2431 2461 2827 2857
Size: 901 30 364 30 1106 30 366 30
Cuts at: 2857 3252
Size: 395
Fragments arranged by size:
1106 901 395 366 364 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1582 1591 1594 2140 2428 3238 3252
Size: 1582 9 3 546 288 810 14
Fragments arranged by size:
1582 810 546 288 14 9 3
BseYI C'CCAG_C
Cuts at: 0 85 898 961 1065 2540 2563 2976 3252
Size: 85 813 63 104 1475 23 413 276
Fragments arranged by size:
1475 813 413 276 104 85 63 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 1355 1687 1948 3252
Size: 894 461 332 261 1304
Fragments arranged by size:
1304 894 461 332 261
BsiEI CG_ry'CG
Cuts at: 0 1019 2753 2898 3252
Size: 1019 1734 145 354
Fragments arranged by size:
1734 1019 354 145
BsiHKAI G_wGCw'C
Cuts at: 0 7 224 332 1195 1305 1586 1595 2002
Size: 7 217 108 863 110 281 9 407
Cuts at: 2002 2033 2144 2297 2422 2525 3252
Size: 31 111 153 125 103 727
Fragments arranged by size:
863 727 407 281 217 153 125 111
110 108 103 31 9 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 970 1564 2065 2702 3154 3252
Size: 970 594 501 637 452 98
Fragments arranged by size:
970 637 594 501 452 98
Bsp1286I G_dGCh'C
Cuts at: 0 7 143 224 332 1099 1195 1305 1586
Size: 7 136 81 108 767 96 110 281
Cuts at: 1586 1595 1616 1685 1715 1937 2002 2033 2144
Size: 9 21 69 30 222 65 31 111
Cuts at: 2144 2297 2422 2525 2628 2644 2651 3230 3252
Size: 153 125 103 103 16 7 579 22
Fragments arranged by size:
767 579 281 222 153 136 125 111
110 108 103 103 96 81 69 65
31 30 22 21 16 9 7 7
BspEI T'CCGG_A
Cuts at: 0 892 3252
Size: 892 2360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 1659 2011 3252
Size: 158 1501 352 1241
Fragments arranged by size:
1501 1241 352 158
BsrBI CCG'CTC
Cuts at: 0 656 1021 1209 3252
Size: 656 365 188 2043
Fragments arranged by size:
2043 656 365 188
BsrFI r'CCGG_y
Cuts at: 0 189 1081 1269 1625 1660 1843 2186 2683
Size: 189 892 188 356 35 183 343 497
Cuts at: 2683 3252
Size: 569
Fragments arranged by size:
892 569 497 356 343 189 188 183
35
BsrGI T'GTAC_A
Cuts at: 0 2573 3252
Size: 2573 679
BssSI C'ACGA_G
Cuts at: 0 289 310 325 1004 1966 3252
Size: 289 21 15 679 962 1286
Fragments arranged by size:
1286 962 679 289 21 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 210 378 453 1503 1761 3252
Size: 210 168 75 1050 258 1491
Fragments arranged by size:
1491 1050 258 210 168 75
BstEII G'GTnAC_C
Cuts at: 0 3165 3252
Size: 3165 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 22 93 3252
Size: 22 71 3159
Fragments arranged by size:
3159 71 22
BstYI r'GATC_y
Cuts at: 0 248 260 1802 2042 2498 3065 3252
Size: 248 12 1542 240 456 567 187
Fragments arranged by size:
1542 567 456 248 240 187 12
BtgI C'CryG_G
Cuts at: 0 104 143 494 1717 1832 2103 2426 2450
Size: 104 39 351 1223 115 271 323 24
Cuts at: 2450 2456 2526 2702 2804 3047 3173 3188 3230
Size: 6 70 176 102 243 126 15 42
Cuts at: 3230 3252
Size: 22
Fragments arranged by size:
1223 351 323 271 243 176 126 115
104 102 70 42 39 24 22 15
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 252 1025 1709 3252
Size: 252 773 684 1543
Fragments arranged by size:
1543 773 684 252
BtsI GCAGTG_nn'
Cuts at: 0 143 172 576 830 1303 3252
Size: 143 29 404 254 473 1949
Fragments arranged by size:
1949 473 404 254 143 29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2808 2843 3252
Size: 2808 35 409
Fragments arranged by size:
2808 409 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2566 3252
Size: 2566 686
EaeI y'GGCC_r
Cuts at: 0 33 101 356 497 779 1016 1866 2354
Size: 33 68 255 141 282 237 850 488
Cuts at: 2354 2453 2895 3252
Size: 99 442 357
Fragments arranged by size:
850 488 442 357 282 255 237 141
99 68 33
EagI C'GGCC_G
Cuts at: 0 1016 2895 3252
Size: 1016 1879 357
Fragments arranged by size:
1879 1016 357
EarI CTCTTCn'nnn_
Cuts at: 0 1241 1697 1771 3230 3252
Size: 1241 456 74 1459 22
Fragments arranged by size:
1459 1241 456 74 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 988 1404 2380 2835 3252
Size: 988 416 976 455 417
Fragments arranged by size:
988 976 455 417 416
EcoICRI GAG'CTC
Cuts at: 0 5 222 1584 3252
Size: 5 217 1362 1668
Fragments arranged by size:
1668 1362 217 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 448 1126 1279 1489 1752 1810 2008
Size: 421 27 678 153 210 263 58 198
Cuts at: 2008 2143 2389 2715 3252
Size: 135 246 326 537
Fragments arranged by size:
678 537 421 326 263 246 210 198
153 135 58 27
EcoO109I rG'GnC_Cy
Cuts at: 0 2537 2647 3252
Size: 2537 110 605
Fragments arranged by size:
2537 605 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2394 3252
Size: 2394 858
HaeII r_GCGC'y
Cuts at: 0 112 232 556 884 1743 3087 3252
Size: 112 120 324 328 859 1344 165
Fragments arranged by size:
1344 859 328 324 165 120 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1330 1362 3117 3149 3252
Size: 1330 32 1755 32 103
Fragments arranged by size:
1755 1330 103 32 32
HincII GTy'rAC
Cuts at: 0 665 2670 3252
Size: 665 2005 582
Fragments arranged by size:
2005 665 582
HindIII A'AGCT_T
Cuts at: 0 2467 3252
Size: 2467 785
KpnI G_GTAC'C
Cuts at: 0 2865 3252
Size: 2865 387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 3252
Size: 833 2419
MscI TGG'CCA
Cuts at: 0 103 358 499 2356 3252
Size: 103 255 141 1857 896
Fragments arranged by size:
1857 896 255 141 103
MslI CAynn'nnrTG
Cuts at: 0 20 508 1852 1981 2425 3172 3252
Size: 20 488 1344 129 444 747 80
Fragments arranged by size:
1344 747 488 444 129 80 20
MspA1I CmG'CkG
Cuts at: 0 210 393 1407 1800 1812 2105 2621 2877
Size: 210 183 1014 393 12 293 516 256
Cuts at: 2877 3112 3252
Size: 235 140
Fragments arranged by size:
1014 516 393 293 256 235 210 183
140 12
NaeI GCC'GGC
Cuts at: 0 191 1271 1662 1845 3252
Size: 191 1080 391 183 1407
Fragments arranged by size:
1407 1080 391 191 183
NcoI C'CATG_G
Cuts at: 0 143 1832 2426 2526 2702 3047 3173 3230
Size: 143 1689 594 100 176 345 126 57
Cuts at: 3230 3252
Size: 22
Fragments arranged by size:
1689 594 345 176 143 126 100 57
22
NdeI CA'TA_TG
Cuts at: 0 2281 3252
Size: 2281 971
NgoMIV G'CCGG_C
Cuts at: 0 189 1269 1660 1843 3252
Size: 189 1080 391 183 1409
Fragments arranged by size:
1409 1080 391 189 183
NspI r_CATG'y
Cuts at: 0 84 2580 2963 3252
Size: 84 2496 383 289
Fragments arranged by size:
2496 383 289 84
PciI A'CATG_T
Cuts at: 0 2576 3252
Size: 2576 676
PflMI CCAn_nnn'nTGG
Cuts at: 0 48 3252
Size: 48 3204
PfoI T'CCnGG_A
Cuts at: 0 919 3252
Size: 919 2333
PpuMI rG'GwC_Cy
Cuts at: 0 2537 3252
Size: 2537 715
PspOMI G'GGCC_C
Cuts at: 0 2647 3252
Size: 2647 605
PspXI vC'TCGA_Gb
Cuts at: 0 2914 3252
Size: 2914 338
PstI C_TGCA'G
Cuts at: 0 215 458 1244 1433 1508 2069 2477 3095
Size: 215 243 786 189 75 561 408 618
Cuts at: 3095 3252
Size: 157
Fragments arranged by size:
786 618 561 408 243 215 189 157
75
PvuI CG_AT'CG
Cuts at: 0 2753 3252
Size: 2753 499
PvuII CAG'CTG
Cuts at: 0 210 1812 2877 3252
Size: 210 1602 1065 375
Fragments arranged by size:
1602 1065 375 210
SacI G_AGCT'C
Cuts at: 0 7 224 1586 3252
Size: 7 217 1362 1666
Fragments arranged by size:
1666 1362 217 7
SacII CC_GC'GG
Cuts at: 0 2106 3252
Size: 2106 1146
SalI G'TCGA_C
Cuts at: 0 663 3252
Size: 663 2589
SanDI GG'GwC_CC
Cuts at: 0 2537 3252
Size: 2537 715
SapI GCTCTTCn'nnn_
Cuts at: 0 1771 3252
Size: 1771 1481
SbfI CC_TGCA'GG
Cuts at: 0 2069 3252
Size: 2069 1183
ScaI AGT'ACT
Cuts at: 0 2719 3079 3252
Size: 2719 360 173
Fragments arranged by size:
2719 360 173
SexAI A'CCwGG_T
Cuts at: 0 1436 3252
Size: 1436 1816
SfcI C'TryA_G
Cuts at: 0 211 454 1240 1429 1504 2065 2131 2473
Size: 211 243 786 189 75 561 66 342
Cuts at: 2473 2553 3091 3252
Size: 80 538 161
Fragments arranged by size:
786 561 538 342 243 211 189 161
80 75 66
SgrAI Cr'CCGG_yG
Cuts at: 0 2683 3252
Size: 2683 569
SmaI CCC'GGG
Cuts at: 0 1885 3252
Size: 1885 1367
SmlI C'TyrA_G
Cuts at: 0 232 463 2914 3252
Size: 232 231 2451 338
Fragments arranged by size:
2451 338 232 231
StuI AGG'CCT
Cuts at: 0 1295 1875 1964 3252
Size: 1295 580 89 1288
Fragments arranged by size:
1295 1288 580 89
StyI C'CwwG_G
Cuts at: 0 143 1202 1325 1832 2426 2526 2702 3047
Size: 143 1059 123 507 594 100 176 345
Cuts at: 3047 3173 3230 3252
Size: 126 57 22
Fragments arranged by size:
1059 594 507 345 176 143 126 123
100 57 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 921 1186 3252
Size: 921 265 2066
Fragments arranged by size:
2066 921 265
TatI w'GTAC_w
Cuts at: 0 2573 2717 3077 3252
Size: 2573 144 360 175
Fragments arranged by size:
2573 360 175 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 568 600 1295 1306 1327 1338 3252
Size: 568 32 695 11 21 11 1914
Fragments arranged by size:
1914 695 568 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 0 1395 3252
Size: 1395 1857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1062 3252
Size: 1062 2190
XhoI C'TCGA_G
Cuts at: 0 2914 3252
Size: 2914 338
XmaI C'CCGG_G
Cuts at: 0 1883 3252
Size: 1883 1369
XmnI GAAnn'nnTTC
Cuts at: 0 2608 3252
Size: 2608 644
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PpuMI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgrAI SmaI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AlfI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BsaAI BsiWI BsmI BspHI BsrDI
BssHII BstBI BstZ17I Bsu36I ClaI DraI DraIII EcoNI
EcoRI EcoRV FseI FspI FspAI HpaI KasI MfeI
MluI NarI NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PsiI PsrI RsrII SfiI
SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI
XbaI ZraI