Restriction map for
pFN21AA0259
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BcnI BfrI BlnI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BsrBI BssHII BssSI
BstEII BstPI BstUI Bsu36I ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FauI FseI FspI HaeII
HgaI HpaI KasI KpnI KspI
MluI MroI MvnI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacI SacII SapI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI SwaI Tth111I XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AspHI 2911 GAGCAC G[AT]GC[AT]^C
AvaII 238 GGTCC G^G[AT]CC
BalI 3116 TGGCCA TGG^CCA
BciVI 2460 GGATAC GTATCC(6/5)
BclI 204 TGATCA T^GATCA
BglI 3958 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1464 AGATCT A^GATCT
BlpI 1552 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1552 GCTGAGC GC^T[ACGT]AGC
BsaAI 3614 TACGTG [CT]AC^GT[GA]
BsaBI 1459 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 4030 GTGCAG GTGCAG(16/14)
BsiHKAI 2911 GAGCAC G[AT]GC[AT]^C
BspHI 3780 TCATGA T^CATGA
BspLU11I 4203 ACATGT A^CATGT
BspMI 2139 ACCTGC ACCTGC(4/8)
BsrFI 3020 GCCGGC [GA]^CCGG[CT]
Bst1107I 403 GTATAC GTA^TAC
BstBI 3938 TTCGAA TT^CGAA
BstXI 1962 CCAGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 403 GTATAC GTA^TAC
CelII 1552 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3020 GCCGGC [GA]^CCGG[CT]
DraII 1528 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoNI 2478 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1528 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 94 GAATTC G^AATTC
EcoRV 2998 GATATC GAT^ATC
FbaI 204 TGATCA T^GATCA
HapII 3021 CCGG C^CGG
HpaII 3021 CCGG C^CGG
MamI 1459 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3116 TGGCCA TGG^CCA
MscI 3116 TGGCCA TGG^CCA
MspI 3021 CCGG C^CGG
NaeI 3020 GCCGGC GCC^GGC
NgoMIV 3020 GCCGGC G^CCGGC
NspV 3938 TTCGAA TT^CGAA
PshAI 3844 GACTATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3780 TCATGA T^CATGA
SalI 3035 GTCGAC G^TCGAC
SbfI 4007 CCTGCAGG CCTGCA^GG
ScaI 2074 AGTACT AGT^ACT
SexAI 4269 ACCTGGT A^CC[AT]GGT
SfuI 3938 TTCGAA TT^CGAA
Sse8387I 4007 CCTGCAGG CCTGCA^GG
StuI 1252 AGGCCT AGG^CCT
XbaI 1029 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 1515 GACGTC GACGT^C
2549 GACGTC
AcyI 1515 GACGTC G[GA]^CG[CT]C
2549 GACGTC
AvaI 79 CTCGAG C^[CT]CG[GA]G
4217 CTCGAG
BanII 1555 GAGCCC G[GA]GC[CT]^C
2443 GAGCCC
BsaHI 1515 GACGTC G[GA]^CG[CT]C
2549 GACGTC
BsaI 410 GAGACC GGTCTC(1/5)
2382 GGTCTC
BsoBI 79 CTCGAG C^[CT]CG[GA]G
4217 CTCGAG
BsrDI 4404 CATTGC GCAATG(2/0)
4120 GCAATG
BstAPI 3463 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4360 GCAGAAGCTGC
BstYI 1464 AGATCT [GA]^GATC[CT]
3078 AGATCC
CfoI 3005 GCGC GCG^C
3302 GCGC
HhaI 3005 GCGC GCG^C
3302 GCGC
Hin1I 1515 GACGTC G[GA]^CG[CT]C
2549 GACGTC
HinP1I 3005 GCGC G^CGC
3302 GCGC
HindIII 217 AAGCTT A^AGCTT
2366 AAGCTT
MfeI 694 CAATTG C^AATTG
914 CAATTG
MflI 1464 AGATCT [GA]^GATC[CT]
3078 AGATCC
MspA1I 3047 CAGCTG C[AC]G^C[GT]G
3968 CAGCTG
MunI 694 CAATTG C^AATTG
914 CAATTG
PaeR7I 79 CTCGAG C^TCGAG
4217 CTCGAG
PvuII 3047 CAGCTG CAG^CTG
3968 CAGCTG
SspI 464 AATATT AAT^ATT
946 AATATT
XcmI 2984 CCAAAATGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3725 CCAACCCCCCTGTGG
XhoI 79 CTCGAG C^TCGAG
4217 CTCGAG
XhoII 1464 AGATCT [GA]^GATC[CT]
3078 AGATCC
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraIII
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XmnI