(Circular) (Six-base) MAPSORT of: pFN21AA0259.seq Check: 5913 from: 1 to: 9271 pFN21AA0259 9271 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3524 4558 7081 278 Size: 53 83 186 936 1988 1034 2523 2468 Fragments arranged by size: 2523 2468 1988 1034 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2409 5041 5112 6460 6614 1937 Size: 472 2632 71 1348 154 4594 Fragments arranged by size: 4594 2632 1348 472 154 71 Acc65I G'GTAC_C Cuts at: 6592 6592 Size: 9271 AclI AA'CG_TT Cuts at: 1563 7398 7771 1563 Size: 5835 373 3063 Fragments arranged by size: 5835 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2037 2105 2510 3090 3339 3546 3669 4424 Size: 405 68 405 580 249 207 123 755 Cuts at: 4424 5507 5649 5673 6233 7334 8718 8986 1632 Size: 1083 142 24 560 1101 1384 268 1917 Fragments arranged by size: 1917 1384 1101 1083 755 580 560 405 405 268 249 207 142 123 68 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9074 AflIII A'CryG_T Cuts at: 1203 5620 6208 6384 6568 8170 9147 1203 Size: 4417 588 176 184 1602 977 1327 Fragments arranged by size: 4417 1602 1327 977 588 184 176 AgeI A'CCGG_T Cuts at: 8102 8102 Size: 9271 AhdI GACnn_n'nnGTC Cuts at: 8000 8000 Size: 9271 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9271 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4328 1184 Size: 3144 6127 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3147 3179 3315 3347 4011 4043 3147 Size: 32 136 32 664 32 8375 Fragments arranged by size: 8375 664 136 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 4289 5396 6371 7014 8586 4289 Size: 1107 975 643 1572 4974 Fragments arranged by size: 4974 1572 1107 975 643 ApaLI G'TGCA_C Cuts at: 7328 8484 7328 Size: 1156 8115 ApoI r'AATT_y Cuts at: 1493 2099 3357 4138 4222 4279 4478 6580 6806 Size: 606 1258 781 84 57 199 2102 226 Cuts at: 6806 6958 9067 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 2102 1697 1258 781 606 226 199 152 84 57 AseI AT'TA_AT Cuts at: 160 1032 7825 160 Size: 872 6793 1606 Fragments arranged by size: 6793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9271 AvaI C'yCGr_G Cuts at: 1870 1943 2084 6222 6596 1870 Size: 73 141 4138 374 4545 Fragments arranged by size: 4545 4138 374 141 73 BamHI G'GATC_C Cuts at: 6601 6968 6601 Size: 367 8904 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6592 8047 618 Size: 325 217 627 4805 1455 1842 Fragments arranged by size: 4805 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3564 4452 6590 1391 Size: 2173 888 2138 4072 Fragments arranged by size: 4072 2173 2138 888 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2043 3145 3600 5052 6051 6115 928 Size: 971 144 1102 455 1452 999 64 4084 Fragments arranged by size: 4084 1452 1102 999 971 455 144 64 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7462 7496 1903 Size: 34 5525 34 3678 Fragments arranged by size: 5525 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4459 7163 8373 4459 Size: 2704 1210 5357 Fragments arranged by size: 5357 2704 1210 BclI T'GATC_A Cuts at: 1548 2209 1548 Size: 661 8610 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5969 7882 136 Size: 107 122 71 631 605 4297 1913 1525 Fragments arranged by size: 4297 1913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3469 9266 3469 Size: 5797 3474 BlpI GC'TnA_GC Cuts at: 3558 6688 3558 Size: 3130 6141 Bme1580I G_kGCm'C Cuts at: 1790 4939 7332 8488 1790 Size: 3149 2393 1156 2573 Fragments arranged by size: 3149 2573 2393 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9271 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4681 5703 7002 Size: 416 53 83 399 24 3264 1022 1299 Cuts at: 7002 7960 442 Size: 958 1753 Fragments arranged by size: 3264 1753 1299 1022 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9271 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5397 6546 5397 Size: 1149 8122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3035 3996 4533 7931 1239 Size: 1796 961 537 3398 2579 Fragments arranged by size: 3398 2579 1796 961 537 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2232 5685 5820 6415 7396 8261 8559 2232 Size: 3453 135 595 981 865 298 2944 Fragments arranged by size: 3453 2944 981 865 595 298 135 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2409 4393 7934 882 Size: 617 910 1984 3541 2219 Fragments arranged by size: 3541 2219 1984 910 617 BsaAI yAC'GTr Cuts at: 493 1204 5621 7062 493 Size: 711 4417 1441 2702 Fragments arranged by size: 4417 2702 1441 711 BsaBI GATnn'nnATC Cuts at: 3468 9265 3468 Size: 5797 3474 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3521 4555 7078 7460 Size: 53 83 186 936 1988 1034 2523 382 Cuts at: 7460 8095 275 Size: 635 1451 Fragments arranged by size: 2523 1988 1451 1034 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7704 8102 8376 8523 8934 7704 Size: 398 274 147 411 8041 Fragments arranged by size: 8041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3147 3177 3819 3849 4011 Size: 30 165 30 1744 30 642 30 162 Cuts at: 4011 4041 4439 4469 5225 5255 5617 5647 6252 Size: 30 398 30 756 30 362 30 605 Cuts at: 6252 6282 1178 Size: 30 4167 Fragments arranged by size: 4167 1744 756 642 605 398 362 165 162 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4911 5474 5792 6273 6429 1186 Size: 3725 563 318 481 156 4028 Fragments arranged by size: 4028 3725 563 481 318 156 BseYI C'CCAG_C Cuts at: 3740 3799 4420 4559 5975 8474 8970 3740 Size: 59 621 139 1416 2499 496 4041 Fragments arranged by size: 4041 2499 1416 621 496 139 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 6056 1163 Size: 573 4320 4378 Fragments arranged by size: 4378 4320 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6721 7482 8510 1540 Size: 275 186 4720 761 1028 2301 Fragments arranged by size: 4720 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4920 7332 7417 8488 4920 Size: 2412 85 1071 5703 Fragments arranged by size: 5703 2412 1071 85 BsmI GAATG_Cn' Cuts at: 6782 6875 6782 Size: 93 9178 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3564 4452 4920 4939 6590 7332 7417 Size: 399 1774 888 468 19 1651 742 85 Cuts at: 7417 8488 1391 Size: 1071 2174 Fragments arranged by size: 2174 1774 1651 1071 888 742 468 399 85 19 BspEI T'CCGG_A Cuts at: 8934 8934 Size: 9271 BspHI T'CATG_A Cuts at: 5785 7160 8890 5785 Size: 1375 1730 6166 Fragments arranged by size: 6166 1730 1375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4153 6626 844 Size: 3309 2473 3489 Fragments arranged by size: 3489 3309 2473 BsrBI CCG'CTC Cuts at: 1394 1823 7158 1394 Size: 429 5335 3507 Fragments arranged by size: 5335 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6132 6408 7766 7940 65 Size: 1183 4884 276 1358 174 1396 Fragments arranged by size: 4884 1396 1358 1183 276 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5025 7915 8102 1700 Size: 110 3215 2890 187 2869 Fragments arranged by size: 3215 2890 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2342 2386 2717 2779 5915 6097 96 Size: 2246 44 331 62 3136 182 3270 Fragments arranged by size: 3270 3136 2246 331 182 62 44 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9271 BssSI C'ACGA_G Cuts at: 7331 8343 7331 Size: 1012 8259 BstAPI GCAn_nnn'nTGC Cuts at: 794 5474 6371 794 Size: 4680 897 3694 Fragments arranged by size: 4680 3694 897 BstBI TT'CG_AA Cuts at: 5944 8075 5944 Size: 2131 7140 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9271 BstXI CCAn_nnnn'nTGG Cuts at: 3974 3974 Size: 9271 BstYI r'GATC_y Cuts at: 1972 3469 5083 6601 6968 7353 7370 8822 8908 Size: 1497 1614 1518 367 385 17 1452 86 Cuts at: 8908 8920 9266 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1614 1518 1497 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2410 2410 Size: 9271 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 8719 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2293 3700 5978 487 Size: 683 56 1067 1407 2278 3780 Fragments arranged by size: 3780 2278 1407 1067 683 56 BtsI GCAGTG_nn' Cuts at: 5019 5350 5495 5903 6787 7581 7601 5019 Size: 331 145 408 884 794 20 6689 Fragments arranged by size: 6689 884 794 408 331 145 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3490 3525 529 Size: 35 2926 35 6275 Fragments arranged by size: 6275 2926 35 35 DraI TTT'AAA Cuts at: 2046 2077 3399 6298 6575 2046 Size: 31 1322 2899 277 4742 Fragments arranged by size: 4742 2899 1322 277 31 DraIII CAC_nnn'GTG Cuts at: 2788 2799 6034 2788 Size: 11 3235 6025 Fragments arranged by size: 6025 3235 11 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8278 809 Size: 507 6962 1802 Fragments arranged by size: 6962 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4853 5023 5121 Size: 54 1425 50 275 18 3023 170 98 Cuts at: 5121 6718 7607 9236 8 Size: 1597 889 1629 43 Fragments arranged by size: 3023 1629 1597 1425 889 275 170 98 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6718 1537 Size: 275 4906 4090 Fragments arranged by size: 4906 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 3908 4505 7201 1353 Size: 780 1775 597 2696 3423 Fragments arranged by size: 3423 2696 1775 780 597 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2415 7858 8232 8378 387 Size: 1064 138 826 5443 374 146 1280 Fragments arranged by size: 5443 1280 1064 826 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2105 2510 3035 3090 3339 3546 Size: 393 405 68 405 525 55 249 207 Cuts at: 3546 3669 3996 4424 4533 5507 5649 5673 6233 Size: 123 327 428 109 974 142 24 560 Cuts at: 6233 7334 7931 8718 8986 1239 Size: 1101 597 787 268 1524 Fragments arranged by size: 1524 1101 974 787 597 560 525 428 405 405 393 327 268 249 207 142 123 109 68 55 24 EcoNI CCTnn'n_nnAGG Cuts at: 4487 4487 Size: 9271 EcoO109I rG'GnC_Cy Cuts at: 3534 3534 Size: 9271 EcoRI G'AATT_C Cuts at: 2099 6580 6958 2099 Size: 4481 378 4412 Fragments arranged by size: 4481 4412 378 EcoRV GAT'ATC Cuts at: 5005 5005 Size: 9271 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2135 3410 3448 4528 5917 2135 Size: 1275 38 1080 1389 5489 Fragments arranged by size: 5489 1389 1275 1080 38 FspI TGC'GCA Cuts at: 7777 7777 Size: 9271 HaeII r_GCGC'y Cuts at: 1678 8418 1678 Size: 6740 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3461 3493 3875 3907 4457 Size: 32 135 32 1889 32 382 32 550 Cuts at: 4457 4489 4673 4705 4865 4897 5225 5257 5617 Size: 32 184 32 160 32 328 32 360 Cuts at: 5617 5649 7912 7944 7986 8018 1373 Size: 32 2263 32 42 32 2626 Fragments arranged by size: 2626 2263 1889 550 382 360 328 184 160 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5042 5404 5530 6615 6861 669 Size: 988 281 3104 362 126 1085 246 3079 Fragments arranged by size: 3104 3079 1085 988 362 281 246 126 HindIII A'AGCT_T Cuts at: 748 2222 4371 748 Size: 1474 2149 5648 Fragments arranged by size: 5648 2149 1474 HpaI GTT'AAC Cuts at: 6861 6861 Size: 9271 KpnI G_GTAC'C Cuts at: 6596 6596 Size: 9271 MfeI C'AATT_G Cuts at: 2699 2919 6870 2699 Size: 220 3951 5100 Fragments arranged by size: 5100 3951 220 MluI A'CGCG_T Cuts at: 9147 9147 Size: 9271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2982 3071 4149 8385 8569 1305 Size: 1677 89 1078 4236 184 2007 Fragments arranged by size: 4236 2007 1677 1078 184 89 MscI TGG'CCA Cuts at: 10 64 1489 1832 5123 10 Size: 54 1425 343 3291 4158 Fragments arranged by size: 4158 3291 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2382 5621 5927 7229 7588 7747 518 Size: 648 1216 3239 306 1302 359 159 2042 Fragments arranged by size: 3239 2042 1302 1216 648 359 306 159 MspA1I CmG'CkG Cuts at: 1606 5054 5975 6687 7364 8512 8757 1606 Size: 3448 921 712 677 1148 245 2120 Fragments arranged by size: 3448 2120 1148 921 712 677 245 NaeI GCC'GGC Cuts at: 5027 5027 Size: 9271 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8719 NdeI CA'TA_TG Cuts at: 387 387 Size: 9271 NgoMIV G'CCGG_C Cuts at: 5025 5025 Size: 9271 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9271 NotI GC'GGCC_GC Cuts at: 6718 6718 Size: 9271 NspI r_CATG'y Cuts at: 1132 1229 4072 4400 6212 6629 8174 1132 Size: 97 2843 328 1812 417 1545 2229 Fragments arranged by size: 2843 2229 1812 1545 417 328 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9271 PciI A'CATG_T Cuts at: 6208 8170 6208 Size: 1962 7309 PflMI CCAn_nnn'nTGG Cuts at: 1108 2772 2799 3352 5153 1108 Size: 1664 27 553 1801 5226 Fragments arranged by size: 5226 1801 1664 553 27 PfoI T'CCnGG_A Cuts at: 4549 5608 4549 Size: 1059 8212 PmeI GTTT'AAAC Cuts at: 6575 6575 Size: 9271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3147 3179 4011 4043 7340 7372 8853 8885 3147 Size: 32 832 32 3297 32 1481 32 3533 Fragments arranged by size: 3533 3297 1481 832 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 5853 1613 Size: 4240 5031 PsiI TTA'TAA Cuts at: 2310 4983 5768 6841 2310 Size: 2673 785 1073 4740 Fragments arranged by size: 4740 2673 1073 785 PstI C_TGCA'G Cuts at: 830 2234 4294 6017 6623 830 Size: 1404 2060 1723 606 3478 Fragments arranged by size: 3478 2060 1723 1404 606 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9271 PvuII CAG'CTG Cuts at: 5054 5975 5054 Size: 921 8350 SalI G'TCGA_C Cuts at: 1936 5040 6613 1936 Size: 3104 1573 4594 Fragments arranged by size: 4594 3104 1573 SbfI CC_TGCA'GG Cuts at: 6017 6623 6017 Size: 606 8665 ScaI AGT'ACT Cuts at: 4081 7519 9231 4081 Size: 3438 1712 4121 Fragments arranged by size: 4121 3438 1712 SexAI A'CCwGG_T Cuts at: 6274 6274 Size: 9271 SfcI C'TryA_G Cuts at: 826 1045 2230 4155 4290 5448 6013 6534 6619 Size: 219 1185 1925 135 1158 565 521 85 Cuts at: 6619 7754 8435 8626 826 Size: 1135 681 191 1471 Fragments arranged by size: 1925 1471 1185 1158 1135 681 565 521 219 191 135 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9271 SmaI CCC'GGG Cuts at: 1872 6598 1872 Size: 4726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 2247 5700 5835 6222 6394 Size: 197 926 141 163 3453 135 387 172 Cuts at: 6394 7375 8276 8538 820 Size: 981 901 262 1553 Fragments arranged by size: 3453 1553 981 926 901 387 262 197 172 163 141 135 SnaBI TAC'GTA Cuts at: 493 7062 493 Size: 6569 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9271 SphI G_CATG'C Cuts at: 1132 6629 1132 Size: 5497 3774 SspI AAT'ATT Cuts at: 5 52 2471 2953 7195 5 Size: 47 2419 482 4242 2081 Fragments arranged by size: 4242 2419 2081 482 47 StuI AGG'CCT Cuts at: 1517 3259 1517 Size: 1742 7529 StyI C'CwwG_G Cuts at: 513 1065 4015 4488 4539 5266 6710 513 Size: 552 2950 473 51 727 1444 3074 Fragments arranged by size: 3074 2950 1444 727 552 473 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5658 5974 6180 7298 7315 7468 7653 Size: 1255 4078 316 206 1118 17 153 185 Cuts at: 7653 8098 325 Size: 445 1498 Fragments arranged by size: 4078 1498 1255 1118 445 316 206 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2342 2386 2717 Size: 274 80 33 51 1443 365 44 331 Cuts at: 2717 2779 4079 4900 5805 5915 6097 6387 6565 Size: 62 1300 821 905 110 182 290 178 Cuts at: 6565 7517 9229 96 Size: 952 1712 138 Fragments arranged by size: 1712 1443 1300 952 905 821 365 331 290 274 182 178 138 110 80 62 51 44 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3898 3930 4895 4927 6018 6050 6252 6284 9231 Size: 32 965 32 1091 32 202 32 2947 Cuts at: 9231 9263 3898 Size: 32 3906 Fragments arranged by size: 3906 2947 1091 965 202 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9271 XbaI T'CTAG_A Cuts at: 3034 6607 3034 Size: 3573 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4996 5737 4996 Size: 741 8530 XhoI C'TCGA_G Cuts at: 1943 2084 6222 1943 Size: 141 4138 4992 Fragments arranged by size: 4992 4138 141 XmaI C'CCGG_G Cuts at: 1870 6596 1870 Size: 4726 4545 XmnI GAAnn'nnTTC Cuts at: 2118 2373 6064 7400 2118 Size: 255 3691 1336 3989 Fragments arranged by size: 3989 3691 1336 255 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3522 4556 7079 276 Size: 53 83 186 936 1988 1034 2523 2468 Fragments arranged by size: 2523 2468 1988 1034 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI