Restriction map for
pFN21AA0259
(Linear) (Six-base) MAPSORT of: KIAA0259.seq Check: 9001 from: 1 to: 4566
KIAA0259.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AatII G_ACGT'C
Cuts at: 0 1519 2553 4566
Size: 1519 1034 2013
Fragments arranged by size:
2013 1519 1034
AccI GT'mk_AC
Cuts at: 0 404 3036 3107 4455 4566
Size: 404 2632 71 1348 111
Fragments arranged by size:
2632 1348 404 111 71
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 100 505 1085 1334 1541 1664 2419
Size: 32 68 405 580 249 207 123 755
Cuts at: 2419 3502 3644 3668 4228 4566
Size: 1083 142 24 560 338
Fragments arranged by size:
1083 755 580 560 405 338 249 207
142 123 68 32 24
AflIII A'CryG_T
Cuts at: 0 3615 4203 4379 4566
Size: 3615 588 176 187
Fragments arranged by size:
3615 588 187 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2323 4566
Size: 2323 2243
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1142 1174 1310 1342 2006 2038 4566
Size: 1142 32 136 32 664 32 2528
Fragments arranged by size:
2528 1142 664 136 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2284 3391 4366 4566
Size: 2284 1107 975 200
Fragments arranged by size:
2284 1107 975 200
ApoI r'AATT_y
Cuts at: 0 94 1352 2133 2217 2274 2473 4566
Size: 94 1258 781 84 57 199 2093
Fragments arranged by size:
2093 1258 781 199 94 84 57
AvaI C'yCGr_G
Cuts at: 0 79 4217 4566
Size: 79 4138 349
Fragments arranged by size:
4138 349 79
BanII G_rGCy'C
Cuts at: 0 1559 2447 4566
Size: 1559 888 2119
Fragments arranged by size:
2119 1559 888
BbsI GAAGACnn'nnnn_
Cuts at: 0 38 1140 1595 3047 4046 4110 4566
Size: 38 1102 455 1452 999 64 456
Fragments arranged by size:
1452 1102 999 456 455 64 38
BciVI GTATCCnnnnn_n'
Cuts at: 0 2454 4566
Size: 2454 2112
BclI T'GATC_A
Cuts at: 0 204 4566
Size: 204 4362
BglI GCCn_nnn'nGGC
Cuts at: 0 3964 4566
Size: 3964 602
BglII A'GATC_T
Cuts at: 0 1464 4566
Size: 1464 3102
BlpI GC'TnA_GC
Cuts at: 0 1553 4566
Size: 1553 3013
Bme1580I G_kGCm'C
Cuts at: 0 2934 4566
Size: 2934 1632
BmrI ACTGGGnnnn_n'
Cuts at: 0 2676 3698 4566
Size: 2676 1022 868
Fragments arranged by size:
2676 1022 868
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3392 4541 4566
Size: 3392 1149 25
Fragments arranged by size:
3392 1149 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 1991 2528 4566
Size: 1030 961 537 2038
Fragments arranged by size:
2038 1030 961 537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 3680 3815 4410 4566
Size: 227 3453 135 595 156
Fragments arranged by size:
3453 595 227 156 135
BsaI GGTCTCn'nnnn_
Cuts at: 0 404 2388 4566
Size: 404 1984 2178
Fragments arranged by size:
2178 1984 404
BsaAI yAC'GTr
Cuts at: 0 3616 4566
Size: 3616 950
BsaBI GATnn'nnATC
Cuts at: 0 1463 4566
Size: 1463 3103
BsaHI Gr'CG_yC
Cuts at: 0 1516 2550 4566
Size: 1516 1034 2016
Fragments arranged by size:
2016 1516 1034
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1142 1172 1814 1844 2006 2036 2434 2464
Size: 1142 30 642 30 162 30 398 30
Cuts at: 2464 3220 3250 3612 3642 4247 4277 4566
Size: 756 30 362 30 605 30 289
Fragments arranged by size:
1142 756 642 605 398 362 289 162
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2906 3469 3787 4268 4424 4566
Size: 2906 563 318 481 156 142
Fragments arranged by size:
2906 563 481 318 156 142
BseYI C'CCAG_C
Cuts at: 0 1735 1794 2415 2554 3970 4566
Size: 1735 59 621 139 1416 596
Fragments arranged by size:
1735 1416 621 596 139 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4051 4566
Size: 4051 515
BsiHKAI G_wGCw'C
Cuts at: 0 2915 4566
Size: 2915 1651
Bsp1286I G_dGCh'C
Cuts at: 0 1559 2447 2915 2934 4566
Size: 1559 888 468 19 1632
Fragments arranged by size:
1632 1559 888 468 19
BspHI T'CATG_A
Cuts at: 0 3780 4566
Size: 3780 786
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2148 4566
Size: 2148 2418
BsrDI GCAATG_nn'
Cuts at: 0 4127 4403 4566
Size: 4127 276 163
Fragments arranged by size:
4127 276 163
BsrFI r'CCGG_y
Cuts at: 0 3020 4566
Size: 3020 1546
BsrGI T'GTAC_A
Cuts at: 0 337 381 712 774 3910 4092 4566
Size: 337 44 331 62 3136 182 474
Fragments arranged by size:
3136 474 337 331 182 62 44
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3469 4366 4566
Size: 3469 897 200
Fragments arranged by size:
3469 897 200
BstBI TT'CG_AA
Cuts at: 0 3939 4566
Size: 3939 627
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1969 4566
Size: 1969 2597
BstYI r'GATC_y
Cuts at: 0 1464 3078 4566
Size: 1464 1614 1488
Fragments arranged by size:
1614 1488 1464
BstZ17I GTA'TAC
Cuts at: 0 405 4566
Size: 405 4161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 288 1695 3973 4566
Size: 288 1407 2278 593
Fragments arranged by size:
2278 1407 593 288
BtsI GCAGTG_nn'
Cuts at: 0 3014 3345 3490 3898 4566
Size: 3014 331 145 408 668
Fragments arranged by size:
3014 668 408 331 145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1485 1520 4566
Size: 1485 35 3046
Fragments arranged by size:
3046 1485 35
DraI TTT'AAA
Cuts at: 0 41 72 1394 4293 4566
Size: 41 31 1322 2899 273
Fragments arranged by size:
2899 1322 273 41 31
DraIII CAC_nnn'GTG
Cuts at: 0 783 794 4029 4566
Size: 783 11 3235 537
Fragments arranged by size:
3235 783 537 11
EaeI y'GGCC_r
Cuts at: 0 2848 3018 3116 4566
Size: 2848 170 98 1450
Fragments arranged by size:
2848 1450 170 98
EarI CTCTTCn'nnn_
Cuts at: 0 128 1903 2500 4566
Size: 128 1775 597 2066
Fragments arranged by size:
2066 1775 597 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 410 4566
Size: 410 4156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 100 505 1030 1085 1334 1541 1664
Size: 32 68 405 525 55 249 207 123
Cuts at: 1664 1991 2419 2528 3502 3644 3668 4228 4566
Size: 327 428 109 974 142 24 560 338
Fragments arranged by size:
974 560 525 428 405 338 327 249
207 142 123 109 68 55 32 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2482 4566
Size: 2482 2084
EcoO109I rG'GnC_Cy
Cuts at: 0 1529 4566
Size: 1529 3037
EcoRI G'AATT_C
Cuts at: 0 94 4566
Size: 94 4472
EcoRV GAT'ATC
Cuts at: 0 3000 4566
Size: 3000 1566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 130 1405 1443 2523 3912 4566
Size: 130 1275 38 1080 1389 654
Fragments arranged by size:
1389 1275 1080 654 130 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1456 1488 1870 1902 2452 2484 2668 2700
Size: 1456 32 382 32 550 32 184 32
Cuts at: 2700 2860 2892 3220 3252 3612 3644 4566
Size: 160 32 328 32 360 32 922
Fragments arranged by size:
1456 922 550 382 360 328 184 160
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3037 3399 3525 4566
Size: 3037 362 126 1041
Fragments arranged by size:
3037 1041 362 126
HindIII A'AGCT_T
Cuts at: 0 217 2366 4566
Size: 217 2149 2200
Fragments arranged by size:
2200 2149 217
MfeI C'AATT_G
Cuts at: 0 694 914 4566
Size: 694 220 3652
Fragments arranged by size:
3652 694 220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1066 2144 4566
Size: 977 89 1078 2422
Fragments arranged by size:
2422 1078 977 89
MscI TGG'CCA
Cuts at: 0 3118 4566
Size: 3118 1448
MslI CAynn'nnrTG
Cuts at: 0 377 3616 3922 4566
Size: 377 3239 306 644
Fragments arranged by size:
3239 644 377 306
MspA1I CmG'CkG
Cuts at: 0 3049 3970 4566
Size: 3049 921 596
Fragments arranged by size:
3049 921 596
NaeI GCC'GGC
Cuts at: 0 3022 4566
Size: 3022 1544
NgoMIV G'CCGG_C
Cuts at: 0 3020 4566
Size: 3020 1546
NspI r_CATG'y
Cuts at: 0 2067 2395 4207 4566
Size: 2067 328 1812 359
Fragments arranged by size:
2067 1812 359 328
PciI A'CATG_T
Cuts at: 0 4203 4566
Size: 4203 363
PflMI CCAn_nnn'nTGG
Cuts at: 0 767 794 1347 3148 4566
Size: 767 27 553 1801 1418
Fragments arranged by size:
1801 1418 767 553 27
PfoI T'CCnGG_A
Cuts at: 0 2544 3603 4566
Size: 2544 1059 963
Fragments arranged by size:
2544 1059 963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1142 1174 2006 2038 4566
Size: 1142 32 832 32 2528
Fragments arranged by size:
2528 1142 832 32 32
PshAI GACnn'nnGTC
Cuts at: 0 3848 4566
Size: 3848 718
PsiI TTA'TAA
Cuts at: 0 305 2978 3763 4566
Size: 305 2673 785 803
Fragments arranged by size:
2673 803 785 305
PstI C_TGCA'G
Cuts at: 0 229 2289 4012 4566
Size: 229 2060 1723 554
Fragments arranged by size:
2060 1723 554 229
PvuII CAG'CTG
Cuts at: 0 3049 3970 4566
Size: 3049 921 596
Fragments arranged by size:
3049 921 596
SalI G'TCGA_C
Cuts at: 0 3035 4566
Size: 3035 1531
SbfI CC_TGCA'GG
Cuts at: 0 4012 4566
Size: 4012 554
ScaI AGT'ACT
Cuts at: 0 2076 4566
Size: 2076 2490
SexAI A'CCwGG_T
Cuts at: 0 4269 4566
Size: 4269 297
SfcI C'TryA_G
Cuts at: 0 225 2150 2285 3443 4008 4529 4566
Size: 225 1925 135 1158 565 521 37
Fragments arranged by size:
1925 1158 565 521 225 135 37
SmlI C'TyrA_G
Cuts at: 0 79 242 3695 3830 4217 4389 4566
Size: 79 163 3453 135 387 172 177
Fragments arranged by size:
3453 387 177 172 163 135 79
SspI AAT'ATT
Cuts at: 0 466 948 4566
Size: 466 482 3618
Fragments arranged by size:
3618 482 466
StuI AGG'CCT
Cuts at: 0 1254 4566
Size: 1254 3312
StyI C'CwwG_G
Cuts at: 0 2010 2483 2534 3261 4566
Size: 2010 473 51 727 1305
Fragments arranged by size:
2010 1305 727 473 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3653 3969 4175 4566
Size: 3653 316 206 391
Fragments arranged by size:
3653 391 316 206
TatI w'GTAC_w
Cuts at: 0 337 381 712 774 2074 2895 3800 3910
Size: 337 44 331 62 1300 821 905 110
Cuts at: 3910 4092 4382 4560 4566
Size: 182 290 178 6
Fragments arranged by size:
1300 905 821 337 331 290 182 178
110 62 44 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1893 1925 2890 2922 4013 4045 4247 4279
Size: 1893 32 965 32 1091 32 202 32
Cuts at: 4279 4566
Size: 287
Fragments arranged by size:
1893 1091 965 287 202 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 1029 4566
Size: 1029 3537
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2991 3732 4566
Size: 2991 741 834
Fragments arranged by size:
2991 834 741
XhoI C'TCGA_G
Cuts at: 0 79 4217 4566
Size: 79 4138 349
Fragments arranged by size:
4138 349 79
XmnI GAAnn'nnTTC
Cuts at: 0 113 368 4059 4566
Size: 113 255 3691 507
Fragments arranged by size:
3691 507 255 113
ZraI GAC'GTC
Cuts at: 0 1517 2551 4566
Size: 1517 1034 2015
Fragments arranged by size:
2015 1517 1034
Enzymes that do cut:
AatII AccI AcuI AflIII AlfI AloI AlwNI ApoI
AvaI BanII BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI
BstZ17I BtgZI BtsI CspCI DraI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PciI PflMI PfoI PpiI PshAI PsiI
PstI PvuII SalI SbfI ScaI SexAI SfcI SmlI
SspI StuI StyI TaqII TatI TstI XbaI XcmI
XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BssHII
BssSI BstEII Bsu36I BtgI ClaI DrdI EagI EcoICRI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpuMI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SanDI SapI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I
XmaI